ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI2370 good A KOG2098 RNA processing and modification Predicted N6-adenine RNA methylase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI2370 847745 846174 -524
(524 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGL192w [A] KOG2098 Predicted N6-adenine RNA methylase 550 e-156
Hs21361827 [A] KOG2098 Predicted N6-adenine RNA methylase 293 4e-79
HsM9790095 [A] KOG2098 Predicted N6-adenine RNA methylase 290 5e-78
7301087 [A] KOG2098 Predicted N6-adenine RNA methylase 285 9e-77
At4g10760 [A] KOG2098 Predicted N6-adenine RNA methylase 280 5e-75
Hs20536396 [K] KOG2097 Predicted N6-adenine methylase involved i... 102 1e-21
At4g09980 [K] KOG2097 Predicted N6-adenine methylase involved in... 100 5e-21
7297377 [K] KOG2097 Predicted N6-adenine methylase involved in t... 96 1e-19
>YGL192w [A] KOG2098 Predicted N6-adenine RNA methylase
Length = 600
Score = 550 bits (1417), Expect = e-156
Identities = 289/538 (53%), Positives = 359/538 (66%), Gaps = 24/538 (4%)
Query: 3 LNWGLVDFMLDNYASITASPINGSLHDIYVIYSM--FMQNFLWKNNECGET--YYQFCED 58
+N LV F++ NY I +P++G L D+Y +Y + N+ E + QF D
Sbjct: 2 INDKLVHFLIQNYDDILRAPLSGQLKDVYSLYISGGYDDEMQKLRNDKDEVLQFEQFWND 61
Query: 59 LNKISEVCGGCMNFEDDSGGNIFGQKISFVDGDSLKIL-QKVH-YDQAIKLSLSHTGNKA 116
L I + F+ + +KI ++D SLKIL K H + +K S S K
Sbjct: 62 LQDIIFATPQSIQFDQNLLVADRPEKIVYLDVFSLKILYNKFHAFYYTLKSSSSSCEEKV 121
Query: 117 KQIAIDKDSVQVKERKIESEPILLNKLMGILDQSEIVT----PNAKCLRHLNQILSLELG 172
+ ++ K++ LL +L+G+L+ V+ P + L +L +
Sbjct: 122 SSLTTKPEADSEKDQ-------LLGRLLGVLNWDVNVSNQGLPREQLSNRLQNLLREKPS 174
Query: 173 STKLSKEQAKLNTKRSPFIPICSDPKHVSLLSTVINTVNNKIMSLQGEKLKKIEADCPDI 232
S +L+KE+AK T+ +IPICSD H SLLST + VNNKI+SLQ K+ + + P +
Sbjct: 175 SFQLAKERAKYTTEVIEYIPICSDYSHASLLSTSVYIVNNKIVSLQWSKISACQENHPGL 234
Query: 233 IECSNTHIHFIPNLKPQTDLSLGDCSYLDTCHKLSTCRYVHYLQYYPESLXXXXXXXXXX 292
IEC + IHFIPN+KPQTD+SLGDCSYLDTCHKL+TCRY+HYLQY P L
Sbjct: 235 IECIQSKIHFIPNIKPQTDISLGDCSYLDTCHKLNTCRYIHYLQYIPSCLQERADRETAS 294
Query: 293 ------XXXXYSFYTHGECCSSSFKKLLPPQWIKCDVRKFDFDILGKFSAVIADPAWNIH 346
FYT G C + KK LP QWI+CDVRKFDF +LGKFS VIADPAWNIH
Sbjct: 295 ENKRIRSNVSIPFYTLGNCSAHCIKKALPAQWIRCDVRKFDFRVLGKFSVVIADPAWNIH 354
Query: 347 MNLPYGTCNDNELLQLPFDLLQEEGLLFLWVTGRAIEVGKESLQNWGYEVINEISWIKTN 406
MNLPYGTCND ELL LP LQ+EG++FLWVTGRAIE+GKESL NWGY VINE+SWIKTN
Sbjct: 355 MNLPYGTCNDIELLGLPLHELQDEGIIFLWVTGRAIELGKESLNNWGYNVINEVSWIKTN 414
Query: 407 QLGRTIVTGRTGHWLNHSKEHLLVGVKGNPEWLNRKIDIDLIISATRETSRKPDELYGII 466
QLGRTIVTGRTGHWLNHSKEHLLVG+KGNP+W+N+ ID+DLI+S TRETSRKPDELYGI
Sbjct: 415 QLGRTIVTGRTGHWLNHSKEHLLVGLKGNPKWINKHIDVDLIVSMTRETSRKPDELYGIA 474
Query: 467 ERLVGTHARKLEIFGRDHNIRPGWFTIGNQVTGSSIHEVDVKRKYERFMEKSKNSKTN 524
ERL GTHARKLEIFGRDHN RPGWFTIGNQ+TG+ I+E+DV+RKY+ FM KSK ++
Sbjct: 475 ERLAGTHARKLEIFGRDHNTRPGWFTIGNQLTGNCIYEMDVERKYQEFM-KSKTGTSH 531
>Hs21361827 [A] KOG2098 Predicted N6-adenine RNA methylase
Length = 580
Score = 293 bits (750), Expect = 4e-79
Identities = 146/295 (49%), Positives = 193/295 (64%), Gaps = 11/295 (3%)
Query: 220 EKLKKIEADCPDIIECSNTHIHFIPNLKPQTDLSLGDCSYLDTCHKLSTCRYVHY-LQYY 278
E +K +AD P C H I N TD SLGDCS+L+TC + TC+YVHY +
Sbjct: 283 ECMKASDADRP----CRKLHFRRIIN--KHTDESLGDCSFLNTCFHMDTCKYVHYEIDAC 336
Query: 279 PESLXXXXXXXXXXXXXXYSFYTHGECCSSSFKKLLPPQWIKCDVRKFDFDILGKFSAVI 338
+S + G+ SS +L PPQWI CD+R D ILGKF+ V+
Sbjct: 337 MDSEAPGSKDHTPSQELALTQSVGGD---SSADRLFPPQWICCDIRYLDVSILGKFAVVM 393
Query: 339 ADPAWNIHMNLPYGTCNDNELLQLPFDLLQEEGLLFLWVTGRAIEVGKESLQNWGYEVIN 398
ADP W+IHM LPYGT D+E+ +L +LQ++G LFLWVTGRA+E+G+E L WGYE ++
Sbjct: 394 ADPPWDIHMELPYGTLTDDEMRRLNIPVLQDDGFLFLWVTGRAMELGRECLNLWGYERVD 453
Query: 399 EISWIKTNQLGRTIVTGRTGHWLNHSKEHLLVGVKGNPEWLNRKIDIDLIISATRETSRK 458
EI W+KTNQL R I TGRTGHWLNH KEH LVGVKGNP+ N+ +D D+I++ R TS K
Sbjct: 454 EIIWVKTNQLQRIIRTGRTGHWLNHGKEHCLVGVKGNPQGFNQGLDCDVIVAEVRSTSHK 513
Query: 459 PDELYGIIERLVGTHARKLEIFGRDHNIRPGWFTIGNQVTGSSIHEVDVKRKYER 513
PDE+YG+IERL RK+E+FGR HN++P W T+GNQ+ G + + DV ++++
Sbjct: 514 PDEIYGMIERL-SPGTRKIELFGRPHNVQPNWITLGNQLDGIHLLDPDVVARFKQ 567
>HsM9790095 [A] KOG2098 Predicted N6-adenine RNA methylase
Length = 579
Score = 290 bits (741), Expect = 5e-78
Identities = 145/295 (49%), Positives = 192/295 (64%), Gaps = 11/295 (3%)
Query: 220 EKLKKIEADCPDIIECSNTHIHFIPNLKPQTDLSLGDCSYLDTCHKLSTCRYVHY-LQYY 278
E +K +AD P C H I N TD SLGDCS+L+TC + TC+YVHY +
Sbjct: 282 ECMKASDADRP----CRKLHFRRIIN--KHTDESLGDCSFLNTCFHMDTCKYVHYEIDAC 335
Query: 279 PESLXXXXXXXXXXXXXXYSFYTHGECCSSSFKKLLPPQWIKCDVRKFDFDILGKFSAVI 338
+S + G+ SS +L PPQWI CD+R ILGKF+ V+
Sbjct: 336 MDSEAPGSKDHTPSQELALTQSVGGD---SSADRLFPPQWICCDIRYLVVSILGKFAVVM 392
Query: 339 ADPAWNIHMNLPYGTCNDNELLQLPFDLLQEEGLLFLWVTGRAIEVGKESLQNWGYEVIN 398
ADP W+IHM LPYGT D+E+ +L +LQ++G LFLWVTGRA+E+G+E L WGYE ++
Sbjct: 393 ADPPWDIHMELPYGTLTDDEMRRLNIPVLQDDGFLFLWVTGRAMELGRECLNLWGYERVD 452
Query: 399 EISWIKTNQLGRTIVTGRTGHWLNHSKEHLLVGVKGNPEWLNRKIDIDLIISATRETSRK 458
EI W+KTNQL R I TGRTGHWLNH KEH LVGVKGNP+ N+ +D D+I++ R TS K
Sbjct: 453 EIIWVKTNQLQRIIRTGRTGHWLNHGKEHCLVGVKGNPQGFNQGLDCDVIVAEVRSTSHK 512
Query: 459 PDELYGIIERLVGTHARKLEIFGRDHNIRPGWFTIGNQVTGSSIHEVDVKRKYER 513
PDE+YG+IERL RK+E+FGR HN++P W T+GNQ+ G + + DV ++++
Sbjct: 513 PDEIYGMIERL-SPGTRKIELFGRPHNVQPNWITLGNQLDGIHLLDPDVVARFKQ 566
>7301087 [A] KOG2098 Predicted N6-adenine RNA methylase
Length = 608
Score = 285 bits (730), Expect = 9e-77
Identities = 136/276 (49%), Positives = 188/276 (67%), Gaps = 8/276 (2%)
Query: 238 THIHFIPNLKPQTDLSLGDCSYLDTCHKLSTCRYVHYLQYYPESLXXXXXXXXXXXXXXY 297
T +HF ++ TD SLGDCS+L+TC ++TC+YVHY ++L
Sbjct: 318 TKLHFKKIIQAHTDESLGDCSFLNTCFHMATCKYVHY---EVDTLPHINTNKPTDVKTKL 374
Query: 298 SFYTHGECCSSSFKKLLPPQWIKCDVRKFDFDILGKFSAVIADPAWNIHMNLPYGTCNDN 357
S + + L PPQWI+CD+R D +LGKF+ V+ADP W+IHM LPYGT +D+
Sbjct: 375 SLKRSVDSSCT----LYPPQWIQCDLRFLDMTVLGKFAVVMADPPWDIHMELPYGTMSDD 430
Query: 358 ELLQLPFDLLQEEGLLFLWVTGRAIEVGKESLQNWGYEVINEISWIKTNQLGRTIVTGRT 417
E+ L LQ++GL+FLWVTGRA+E+G++ L+ WGYE ++E+ W+KTNQL R I TGRT
Sbjct: 431 EMRALGVPALQDDGLIFLWVTGRAMELGRDCLKLWGYERVDELIWVKTNQLQRIIRTGRT 490
Query: 418 GHWLNHSKEHLLVGVKGNPEWLNRKIDIDLIISATRETSRKPDELYGIIERLVGTHARKL 477
GHWLNH KEH LVG+KGNP LNR +D D+I++ R TS KPDE+YGIIERL RK+
Sbjct: 491 GHWLNHGKEHCLVGMKGNPTNLNRGLDCDVIVAEVRATSHKPDEIYGIIERL-SPGTRKI 549
Query: 478 EIFGRDHNIRPGWFTIGNQVTGSSIHEVDVKRKYER 513
E+FGR HNI+P W T+GNQ+ G + + ++ ++++
Sbjct: 550 ELFGRPHNIQPNWITLGNQLDGIRLVDPELITQFQK 585
>At4g10760 [A] KOG2098 Predicted N6-adenine RNA methylase
Length = 685
Score = 280 bits (715), Expect = 5e-75
Identities = 139/282 (49%), Positives = 184/282 (64%), Gaps = 17/282 (6%)
Query: 236 SNTHI-----HFIPNLKPQTDLSLGDCSYLDTCHKLSTCRYVHYLQYYPESLXXXXXXXX 290
S +HI HF + TD+SLGDCS+LDTC + TC+YVHY +++
Sbjct: 383 SGSHIACNKRHFRRLIASHTDVSLGDCSFLDTCRHMKTCKYVHYELDMADAMMAGPDKAL 442
Query: 291 XXXXXXYSFYTHGECCSSSFKKLLPPQWIKCDVRKFDFDILGKFSAVIADPAWNIHMNLP 350
Y CS + +L QWI CD+R F DILG F V+ADP W+IHM LP
Sbjct: 443 KPLRADY--------CSEA--ELGEAQWINCDIRSFRMDILGTFGVVMADPPWDIHMELP 492
Query: 351 YGTCNDNELLQLPFDLLQEEGLLFLWVTGRAIEVGKESLQNWGYEVINEISWIKTNQLGR 410
YGT D+E+ L LQ +GL+FLWVTGRA+E+G+E L+ WGY+ + EI W+KTNQL R
Sbjct: 493 YGTMADDEMRTLNVPSLQTDGLIFLWVTGRAMELGRECLELWGYKRVEEIIWVKTNQLQR 552
Query: 411 TIVTGRTGHWLNHSKEHLLVGVKGNPEWLNRKIDIDLIISATRETSRKPDELYGIIERLV 470
I TGRTGHWLNHSKEH LVG+KGNPE +NR ID D+I++ RETSRKPDE+Y ++ER++
Sbjct: 553 IIRTGRTGHWLNHSKEHCLVGIKGNPE-VNRNIDTDVIVAEVRETSRKPDEMYAMLERIM 611
Query: 471 GTHARKLEIFGRDHNIRPGWFTIGNQVTGSSIHEVDVKRKYE 512
ARKLE+F R HN GW ++GNQ+ G + ++ +++
Sbjct: 612 -PRARKLELFARMHNAHAGWLSLGNQLNGVRLINEGLRARFK 652
>Hs20536396 [K] KOG2097 Predicted N6-adenine methylase involved in
transcription regulation
Length = 456
Score = 102 bits (254), Expect = 1e-21
Identities = 65/209 (31%), Positives = 110/209 (52%), Gaps = 20/209 (9%)
Query: 311 KKLLPPQWIKCDVRKFDF-DILGKFSAVIADPAWNIHMNLPYGTCND-----NELLQLPF 364
K PP +++ D+ FD ++ KF ++ +P + T N+ +++++L
Sbjct: 162 KSNTPPMYLQADIEAFDIRELTPKFDVILLEPPLEEYYRETGITANEKCWTWDDIMKLEI 221
Query: 365 D-LLQEEGLLFLWV-TGRAIEVGKESLQNWGYEVINEISWIKTNQL--GRTIVTGRTGHW 420
D + +FLW +G +++G+ L+ WGY +I WIKTN+ G+T +
Sbjct: 222 DEIAAPRSFIFLWCGSGEGLDLGRVCLRKWGYRRCEDICWIKTNKNNPGKTKTLDPKAVF 281
Query: 421 LNHSKEHLLVGVKG------NPEWLNRKIDIDLIISATRETSR--KPDELYGIIERLVGT 472
+KEH L+G+KG + ++++ +DIDLII+ E KP E++ IIE
Sbjct: 282 -QRTKEHCLMGIKGTVKRSTDGDFIHANVDIDLIITEEPEIGNIEKPVEIFHIIEHFC-L 339
Query: 473 HARKLEIFGRDHNIRPGWFTIGNQVTGSS 501
R+L +FGRD IRPGW T+G +T S+
Sbjct: 340 GRRRLHLFGRDSTIRPGWLTVGPTLTNSN 368
>At4g09980 [K] KOG2097 Predicted N6-adenine methylase involved in
transcription regulation
Length = 963
Score = 100 bits (249), Expect = 5e-21
Identities = 62/206 (30%), Positives = 108/206 (52%), Gaps = 21/206 (10%)
Query: 316 PQWIKCDVRKFDF--DILG-KFSAVIADPAWNIHMNLPYGTCNDNE------LLQLPFDL 366
P ++K D+ + + ++ G KF ++ DP W +++ G + E ++ L +
Sbjct: 617 PMYLKGDLHEVELSPELFGTKFDVILVDPPWEEYVHRAPGVSDSMEYWTFEDIINLKIEA 676
Query: 367 LQEE-GLLFLWV-TGRAIEVGKESLQNWGYEVINEISWIKTNQLGRTIVTGRTGHWL-NH 423
+ + LFLWV G +E G++ L+ WG+ +I W+KTN+ +
Sbjct: 677 IADTPSFLFLWVGDGVGLEQGRQCLKKWGFRRCEDICWVKTNKSNAAPTLRHDSRTVFQR 736
Query: 424 SKEHLLVGVKG------NPEWLNRKIDIDLIISATRE--TSRKPDELYGIIERLVGTHAR 475
SKEH L+G+KG + ++ ID D+II+ +++KP+++Y IIE R
Sbjct: 737 SKEHCLMGIKGTVRRSTDGHIIHANIDTDVIIAEEPPYGSTQKPEDMYRIIEHFA-LGRR 795
Query: 476 KLEIFGRDHNIRPGWFTIGNQVTGSS 501
+LE+FG DHNIR GW T+G ++ S+
Sbjct: 796 RLELFGEDHNIRAGWLTVGKGLSSSN 821
>7297377 [K] KOG2097 Predicted N6-adenine methylase involved in
transcription regulation
Length = 397
Score = 95.9 bits (237), Expect = 1e-19
Identities = 66/217 (30%), Positives = 108/217 (49%), Gaps = 37/217 (17%)
Query: 316 PQWIKCDVRKFDFDILG-KFSAVIADPAWNIHMNL----------PYGTCNDNELLQLPF 364
P ++K D++ D LG KF ++ +P + P N +++L L
Sbjct: 139 PMYLKADLKSLDVKTLGAKFDVILIEPPLEEYARAAPSVATVGGAPRVFWNWDDILNLDV 198
Query: 365 -DLLQEEGLLFLWV-TGRAIEVGKESLQNWGYEVINEISWIKTN--------QLGRTIVT 414
++ +FLW + +++G+ L+ WG+ +I WI+TN QL V
Sbjct: 199 GEIAAHRSFVFLWCGSSEGLDMGRNCLKKWGFRRCEDICWIRTNINKPGHSKQLEPKAVF 258
Query: 415 GRTGHWLNHSKEHLLVGVKG------NPEWLNRKIDIDLIISATRE--TSRKPDELYGII 466
RT KEH L+G+KG + ++++ +DIDLIIS E + KP E++ II
Sbjct: 259 QRT-------KEHCLMGIKGTVRRSTDGDFIHANVDIDLIISEEEEFGSFEKPIEIFHII 311
Query: 467 ERLVGTHARKLEIFGRDHNIRPGWFTIGNQVTGSSIH 503
E R+L +FGRD +IRPGW T+G ++T S+ +
Sbjct: 312 EHFC-LGRRRLHLFGRDSSIRPGWLTVGPELTNSNFN 347
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.137 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,570,630
Number of Sequences: 60738
Number of extensions: 1413594
Number of successful extensions: 3221
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 3196
Number of HSP's gapped (non-prelim): 8
length of query: 524
length of database: 30,389,216
effective HSP length: 111
effective length of query: 413
effective length of database: 23,647,298
effective search space: 9766334074
effective search space used: 9766334074
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)