ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactVI2503   suspect: LH   L     KOG2031  Replication, recombination and repair                          Tyrosyl-DNA phosphodiesterase

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI2503 886395 884905 -497 
         (497 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YBR223c [L] KOG2031 Tyrosyl-DNA phosphodiesterase                     117   5e-26
SPCP31B10.05 [L] KOG2031 Tyrosyl-DNA phosphodiesterase                 50   6e-06

>YBR223c [L] KOG2031 Tyrosyl-DNA phosphodiesterase
          Length = 544

 Score =  117 bits (292), Expect = 5e-26
 Identities = 123/466 (26%), Positives = 200/466 (42%), Gaps = 52/466 (11%)

Query: 50  FKVVKSVIFDGDS---RQPDMFELNDVLSDPSLNEVWLFSFGFQINLIMSPIKDHVK-VH 105
           FK++KS  ++ +       DM  L D+    +L    LFSF ++++ ++     +V+ + 
Sbjct: 82  FKLMKSDFYEREDFMGEVEDMITLKDIFGTETLKRSILFSFQYELDFLLRQFHQNVENIT 141

Query: 106 IVGQLNTINSEQPISHYANL-----KVHRVDIPSP-WGCHHSKIIFSFHQNGTMRMHVPS 159
           IVGQ  TI   +  +  A L     KV  ++I  P +  HH+K+I +F+ NG  ++ +PS
Sbjct: 142 IVGQKGTIMPIEARAMDATLAVILKKVKLIEITMPPFASHHTKLIINFYDNGECKIFLPS 201

Query: 160 FNLSREEMNLVQQTVWTSPLLYEKSE--TVPKKKSRFEDEXXXXXXXXXXXXXXXGLIAS 217
            N +  E NL QQ  W SPLL    E   VP K+S  E                  +  S
Sbjct: 202 NNFTSMETNLPQQVCWCSPLLKIGKEGLPVPFKRSLIE-----YLNSYHLKDIDELITKS 256

Query: 218 LKRYKWHVLDEQNCQFVYSTP---YNGGLTQLKSCLRASGMKLHGDEEDDDLSFVNLFIQ 274
           ++   +  L E   +FVYSTP    + GL    + L     KL       D +  +   Q
Sbjct: 257 VEEVNFAPLSE--LEFVYSTPSKFQSSGLLSFYNKLE----KLSAGTSASDTA-KHYLCQ 309

Query: 275 VSSMGNPFRKKFD--LLQDVMIPYLYTDWFEKDGYDKKLKSKEYTTPFLA---------- 322
            SS+G    +  D  L   +MIP L+T        D   + K    P  +          
Sbjct: 310 TSSIGTSLSRARDENLWTHLMIP-LFTGIMSPPAKDTAGRKKAEILPTNSLINEYSQRKI 368

Query: 323 HSTLVWPTKTEIKECMTQGLSANWFFYKRSEQTERKVVPCLRKHVPLPTNATQSDKNRHM 382
              +++PT+ E      +  S+ WF ++  ++     +   +  V    +     + R  
Sbjct: 369 KPYIIFPTEQEFVTSPLKWSSSGWFHFQYLQKKSYYEMLRNKFKVFYKQDPAMVTRRRGT 428

Query: 383 VPSHTKYYIQFTDEN--------TLKRPDWILLTSHNLSQAAWGPSPLKKPTNYECGILY 434
            P+H+K+Y+     +          K  +W L TS NLSQ AWG +  +KP NYE G+LY
Sbjct: 429 TPAHSKFYMHCATNSAGPCDASQVFKELEWCLYTSANLSQTAWG-TVSRKPRNYEAGVLY 487

Query: 435 TTTMGRNKVRLTLASAQQPPGRTIGSRVPEDITVLPTVKVVTPYPL 480
            +    N  ++T  +  +      G+  P  + V  T+ V+ PY L
Sbjct: 488 HSRRLANTRKVTCRTFTRDRRGCAGN--PTHVAVPFTLPVI-PYDL 530


>SPCP31B10.05 [L] KOG2031 Tyrosyl-DNA phosphodiesterase
          Length = 536

 Score = 50.4 bits (119), Expect = 6e-06
 Identities = 31/129 (24%), Positives = 62/129 (48%), Gaps = 19/129 (14%)

Query: 70  LNDVLSDPSLNEVWLFSFGFQINLIMSPIKDHV--------------KVHIVGQLNTINS 115
           L  ++  P L + W F+F   +N ++  +   V              K   + +L    +
Sbjct: 43  LKQLIGSPQLKQTWQFNFCVDLNFLLENMHASVFPTVDVRITHGYDSKSDSLARLTAQMN 102

Query: 116 EQPISHYANLKVHRVDIPSPWGCHHSKIIFSFHQNGTMRMHVPSFNLSREEMNLVQQTVW 175
             P+    N+K++ V +P  WG HHSKI+ +F ++ + ++ + + NL   +   + Q ++
Sbjct: 103 HCPV----NVKLYSVYVPM-WGTHHSKIMVNFFKDDSCQIVIHTANLVEPDWIGMSQAIF 157

Query: 176 TSPLLYEKS 184
            +PLLY K+
Sbjct: 158 KTPLLYPKA 166


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.318    0.134    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,260,457
Number of Sequences: 60738
Number of extensions: 1306986
Number of successful extensions: 2644
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2638
Number of HSP's gapped (non-prelim): 3
length of query: 497
length of database: 30,389,216
effective HSP length: 111
effective length of query: 386
effective length of database: 23,647,298
effective search space: 9127857028
effective search space used: 9127857028
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)