ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI2509 good E KOG0564 Amino acid transport and metabolism 5,10-methylenetetrahydrofolate reductase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI2509 887288 889099 604
(604 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGL125w [E] KOG0564 510-methylenetetrahydrofolate reductase 763 0.0
SPAC56F8.10 [E] KOG0564 510-methylenetetrahydrofolate reductase 488 e-137
Hs6631098 [E] KOG0564 510-methylenetetrahydrofolate reductase 437 e-122
At2g44160 [E] KOG0564 510-methylenetetrahydrofolate reductase 422 e-118
At3g59970 [E] KOG0564 510-methylenetetrahydrofolate reductase 421 e-117
YPL023c [E] KOG0564 510-methylenetetrahydrofolate reductase 364 e-100
SPAC343.10 [E] KOG0564 510-methylenetetrahydrofolate reductase 342 1e-93
CE02451 [E] KOG0564 510-methylenetetrahydrofolate reductase 286 8e-77
>YGL125w [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 599
Score = 763 bits (1969), Expect = 0.0
Identities = 368/606 (60%), Positives = 460/606 (75%), Gaps = 10/606 (1%)
Query: 1 MRISQKLQRAHEEREDATFSFEFFVPKTSQGVQNLYERMDRMYQTQ-PEFIDITWNAGG- 58
M+I++KL++ + T+SFE+FVPKT+QGVQNLY+RMDRMY+ P+FIDITWNAGG
Sbjct: 1 MKITEKLEQHRQTSGKPTYSFEYFVPKTTQGVQNLYDRMDRMYEASLPQFIDITWNAGGG 60
Query: 59 RLSMLSTDLVATSQGVLGLETCMHLTCTNMPVSQIDDALKKAYDAGCENILALRGDPPIN 118
RLS LSTDLVAT+Q VLGLETCMHLTCTNMP+S IDDAL+ AY +GC+NILALRGDPP +
Sbjct: 61 RLSHLSTDLVATAQSVLGLETCMHLTCTNMPISMIDDALENAYHSGCQNILALRGDPPRD 120
Query: 119 SLGEEEEWKPCENGFRYAKDLVSYIREQYGDHFDIGVAAYPEGHPXX-XXXXXXXXXXXX 177
+ E W P E GF+YAKDL+ YI+ +YGDHF IGVA YPE HP
Sbjct: 121 A----ENWTPVEGGFQYAKDLIKYIKSKYGDHFAIGVAGYPECHPELPNKDVKLDLEYLS 176
Query: 178 XVDCGADFMVTQMFYDVDKFLDWCARVREAXXXXXXXXXXXXXTSYPSFIRRTQWCQINV 237
G DF++TQMFYDVD L+WC++VR A T+Y +F+RR QW QI++
Sbjct: 177 RRSTGGDFIITQMFYDVDNLLNWCSQVRAAGMDVPIIPGIMPITTYAAFLRRIQWGQISI 236
Query: 238 PDNFLELLEPVKDDDQLVRDVGTELMVELCQKLLDSGYVNHLHMYTMNLEKASLMILEKL 297
P +F L+P+KDDD+LVRD+GT L+VE+CQKLLDSGYV+HLH+YTMNLEKA LMILE+L
Sbjct: 237 PQHFSSRLDPIKDDDELVRDIGTNLIVEMCQKLLDSGYVSHLHIYTMNLEKAPLMILERL 296
Query: 298 DLLKRSNVGKDVIEPLEPLPWRKSLNPKRRNELVRPIFWQKRPYSYVARTSLWNEDEFPN 357
++L + PL LPWRKSLNPKR+NE VRPIFW++RPYSYVARTS W DEFPN
Sbjct: 297 NILPTESEFN--AHPLAVLPWRKSLNPKRKNEEVRPIFWKRRPYSYVARTSQWAVDEFPN 354
Query: 358 GRFGDSSSPAYGDLDLCGHSLIRQSVKKSIELWSTPQTLDDMSNLVTSYLEGKLKCLPWS 417
GRFGDSSSPA+GDLDLCG LIRQS K +ELWSTP +++D++ LV +YL G LKCLPWS
Sbjct: 355 GRFGDSSSPAFGDLDLCGSDLIRQSANKCLELWSTPTSINDVAFLVINYLNGNLKCLPWS 414
Query: 418 DVPLNHEVDDILKHLIDLNKHHIFTINSQPKVNGKPSTDPVVGWGPTKGYVYQKEYLEFL 477
D+P+N E++ I HLI+LN+H I TINSQP+VNG S D + GWGP GYVYQK+YLEF+
Sbjct: 415 DIPINDEINPIKAHLIELNQHSIITINSQPQVNGIRSNDKIHGWGPKDGYVYQKQYLEFM 474
Query: 478 LPREKLPLFQEICQEHSDHLTYFAIDSQDNLATNHPEDSRANAVTWGVFPGSEILQPTIV 537
LP+ KLP + ++++ LTYFAIDSQ +L +NHP++S++NAVTWG+FPG EILQPTIV
Sbjct: 475 LPKTKLPKLIDTL-KNNEFLTYFAIDSQGDLLSNHPDNSKSNAVTWGIFPGREILQPTIV 533
Query: 538 EKASFLAWKEEFFTILKEWKLTFVEHKATESVNLVNHIIDNYVLINVVDNDFISTNDTIY 597
EK SFLAWKEEF+ IL EWKL ++ S + +ID+Y L+N+VDND+IS +D I+
Sbjct: 534 EKISFLAWKEEFYHILNEWKLNMNKYDKPHSAQFIQSLIDDYCLVNIVDNDYISPDDQIH 593
Query: 598 DLLQQL 603
+L L
Sbjct: 594 SILLSL 599
>SPAC56F8.10 [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 603
Score = 488 bits (1255), Expect = e-137
Identities = 267/604 (44%), Positives = 367/604 (60%), Gaps = 18/604 (2%)
Query: 1 MRISQKLQRAHEEREDATFSFEFFVPKTSQGVQNLYERMDRMYQT-QPEFIDITWNAGGR 59
M+IS KL + +E T+S+EFF PKTS GVQNLY R+DRM +P F+D+TW AGG
Sbjct: 1 MKISDKLLHP-DWKEKVTYSYEFFPPKTSTGVQNLYNRIDRMKTWGRPMFVDVTWGAGGT 59
Query: 60 LSMLSTDLVATSQGVLGLETCMHLTCTNMPVSQIDDALKKAYDAGCENILALRGDPPINS 119
S L+ +V Q ++TCMHLTCTNM ID ALK+A++ GC NILALRGDP
Sbjct: 60 SSELTPGIVNVIQTDFEVDTCMHLTCTNMSTEMIDAALKRAHETGCRNILALRGDPV--- 116
Query: 120 LGEEEEWKPCENGFRYAKDLVSYIREQYGDHFDIGVAAYPEGHPXXXXXXXXXXXXXXXV 179
++ +W E+GFRYA DLV YIR Y D F IGVA YPEG+ V
Sbjct: 117 --KDTDWTEGESGFRYASDLVRYIRTHYNDEFCIGVAGYPEGYSPDDDIDESIKHLKLKV 174
Query: 180 DCGADFMVTQMFYDVDKFLDWCARVREAXXXXXXXXXXXXXTSYPSFIRRTQWCQINVPD 239
D GADF+VTQMFYDVD F+ W +VR A ++ SFIRR +W + +P
Sbjct: 175 DEGADFIVTQMFYDVDNFIAWVDKVRAAGINIPIFPGIMPIQAWDSFIRRAKWSGVKIPQ 234
Query: 240 NFLELLEPVKDDDQLVRDVGTELMVELCQKLLDSGYVNHLHMYTMNLEKASLMILEKLDL 299
+F++ L PVKDDD+ VR+ G EL+VE+C+KL+ SG + LH YTMNLEKA MI+E+L L
Sbjct: 235 HFMDTLVPVKDDDEGVRERGVELIVEMCRKLIASG-ITRLHFYTMNLEKAVKMIIERLGL 293
Query: 300 LKRSNVGKDVIEPLEPLPWRKSLNPKRRNELVRPIFWQKRPYSYVARTSLWNEDEFPNGR 359
L + +++ S +R NE VRPIFW+ R SYV+RT W DE P+GR
Sbjct: 294 LDENLA--PIVDTNNVELTNASSQDRRINEGVRPIFWRTRNESYVSRTDQW--DELPHGR 349
Query: 360 FGDSSSPAYGDLDLCGHSLIRQSVKKSIELWSTPQTLDDMSNLVTSYLEGKLKCLPWSDV 419
+GDS SPA+G+ D + L R S K+ W +P++ ++ +L Y E K+ LPWSD+
Sbjct: 350 WGDSRSPAFGEFDAIRYGL-RMSPKEITTSWGSPKSYSEIGDLFARYCEKKISSLPWSDL 408
Query: 420 PLNHEVDDILKHLIDLNKHHIFTINSQPKVNGKPSTDPVVGWGPTKGYVYQKEYLEFLLP 479
P++ E D I L+ +N++ TINSQP +NG+ S+ PV GWGP GYV+QK Y+EF +
Sbjct: 409 PISDEADLIRDQLLSMNRNAFLTINSQPALNGEKSSHPVFGWGPPNGYVFQKPYVEFFVH 468
Query: 480 REKLPLFQEICQEHSDHLTYFAIDSQDNLATNHPEDSRANAVTWGVFPGSEILQPTIVEK 539
L +E ++ + ++YF + +L TN + NAVTWGVFP EI+QPTIVE
Sbjct: 469 PSLLNELKETVKK-LNSVSYFVTNKNGDLDTNSQYEI-PNAVTWGVFPNREIIQPTIVES 526
Query: 540 ASFLAWKEEFFTILKEWKLTFVEHKATESVNLVNHIIDNYVLINVVDNDFISTNDTIYDL 599
SFLAWK+E +++ EW + S L+ ++ + L +VDNDF +++D+
Sbjct: 527 TSFLAWKDEAYSLGMEWANAYSPDSI--SRKLLVSMMKEWFLCVIVDNDF-QNGQSLFDV 583
Query: 600 LQQL 603
++
Sbjct: 584 FNKM 587
>Hs6631098 [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 656
Score = 437 bits (1125), Expect = e-122
Identities = 240/603 (39%), Positives = 347/603 (56%), Gaps = 35/603 (5%)
Query: 2 RISQKLQRAHEEREDATFSFEFFVPKTSQGVQNLYERMDRMYQTQPEFIDITWNAGGR-- 59
R+ +K++R E D FS EFF P+T++G NL R DRM P +ID+TW+ G
Sbjct: 44 RLREKMRRRLESG-DKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLYIDVTWHPAGDPG 102
Query: 60 --LSMLSTDLVATSQGVLGLETCMHLTCTNMPVSQIDDALKKAYDAGCENILALRGDPPI 117
S + +T+ GLET +H+TC + +I L KA G +NI+ALRGDP
Sbjct: 103 SDKETSSMMIASTAVNYCGLETILHMTCCRQRLEEITGHLHKAKQLGLKNIMALRGDP-- 160
Query: 118 NSLGEEEEWKPCENGFRYAKDLVSYIREQYGDHFDIGVAAYPEGHPXXXXXXXXXXXXXX 177
+G++ W+ E GF YA DLV +IR ++GD+FDI VA YP+GHP
Sbjct: 161 --IGDQ--WEEEEGGFNYAVDLVKHIRSEFGDYFDICVAGYPKGHPEAGSFEADLKHLKE 216
Query: 178 XVDCGADFMVTQMFYDVDKFLDWCARVREAXXXXXXXXXXXXXTSYPSFIRRTQWCQINV 237
V GADF++TQ+F++ D F + + Y S + + ++ V
Sbjct: 217 KVSAGADFIITQLFFEADTFFRFVKACTDMGITCPIVPGIFPIQGYHSLRQLVKLSKLEV 276
Query: 238 PDNFLELLEPVKDDDQLVRDVGTELMVELCQKLLDSGYVNHLHMYTMNLEKASLMILEKL 297
P +++EP+KD+D +R+ G EL V LCQ+LL SG V LH YT+N E A+ +L++L
Sbjct: 277 PQEIKDVIEPIKDNDAAIRNYGIELAVSLCQELLASGLVPGLHFYTLNREMATTEVLKRL 336
Query: 298 DLLKRSNVGKDVIEPLEPLPWRKSLNPKRRNELVRPIFWQKRPYSYVARTSLWNEDEFPN 357
+ +P PLPW S +PKRR E VRPIFW RP SY+ RT W DEFPN
Sbjct: 337 GMWTE--------DPRRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQEW--DEFPN 386
Query: 358 GRFGDSSSPAYGDL-DLCGHSLIRQSVKKSI-ELWSTPQTLD-DMSNLVTSYLEG----- 409
GR+G+SSSPA+G+L D L +S K+ + ++W T + + + YL G
Sbjct: 387 GRWGNSSSPAFGELKDYYLFYLKSKSPKEELLKMWGEELTSEASVFEVFVLYLSGEPNRN 446
Query: 410 --KLKCLPWSDVPLNHEVDDILKHLIDLNKHHIFTINSQPKVNGKPSTDPVVGWGPTKGY 467
K+ CLPW+D PL E + + L+ +N+ I TINSQP +NGKPS+DP+VGWGP+ GY
Sbjct: 447 GHKVTCLPWNDEPLAAETSLLKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGY 506
Query: 468 VYQKEYLEFLLPREKLPLFQEICQEHSDHLTYFAIDSQDNLATNHPEDSRANAVTWGVFP 527
V+QK YLEF RE ++ +++ + Y ++ + TN PE + NAVTWG+FP
Sbjct: 507 VFQKAYLEFFTSRETAEALLQVLKKYELRVNYHLVNVKGENITNAPE-LQPNAVTWGIFP 565
Query: 528 GSEILQPTIVEKASFLAWKEEFFTI-LKEWKLTFVEHKATESVNLVNHIIDNYVLINVVD 586
G EI+QPT+V+ SF+ WK+E F + ++ W + E + ++ + +I DNY L+N+VD
Sbjct: 566 GREIIQPTVVDPVSFMFWKDEAFALWIERWGKLYEEESPSRTI--IQYIHDNYFLVNLVD 623
Query: 587 NDF 589
NDF
Sbjct: 624 NDF 626
>At2g44160 [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 606
Score = 422 bits (1085), Expect = e-118
Identities = 248/615 (40%), Positives = 343/615 (55%), Gaps = 43/615 (6%)
Query: 1 MRISQKLQRAHEEREDATFSFEFFVPKTSQGVQNLYERMDRMYQTQPEFIDITWNAGGRL 60
M++ K+Q +E + A FSFEFF PKT GV NL+ERMDRM P F DITW AGG
Sbjct: 1 MKVIDKIQSLADEGKTA-FSFEFFPPKTEDGVDNLFERMDRMVAYGPTFCDITWGAGGST 59
Query: 61 SMLSTDLVATSQGVLGLETCMHLTCTNMPVSQIDDALKKAYDAGCENILALRGDPPINSL 120
+ L+ D+ + Q V+ +E+ MHLTCTNMPV +ID AL+ G +N+LALRGDPP
Sbjct: 60 ADLTLDIASRMQNVVCVESMMHLTCTNMPVEKIDHALETIRSNGIQNVLALRGDPPHG-- 117
Query: 121 GEEEEWKPCENGFRYAKDLVSYIREQYGDHFDIGVAAYP------------EGHP----- 163
++++ E GF A DLV++IR +YGD+F I VA YP E HP
Sbjct: 118 --QDKFVQVEGGFDCALDLVNHIRSKYGDYFGITVAGYPAKDWIFMCFDISEAHPDVIGE 175
Query: 164 ----XXXXXXXXXXXXXXXVDCGADFMVTQMFYDVDKFLDWCARVREAXXXXXXXXXXXX 219
+D GAD +VTQ+FYD D FL + R+
Sbjct: 176 NGLASNEAYQSDLEYLKKKIDAGADLIVTQLFYDTDIFLKFVNDCRQIGISCPIVPGIMP 235
Query: 220 XTSYPSFIRRTQWCQINVPDNFLELLEPVKDDDQLVRDVGTELMVELCQKLLDSGYVNHL 279
+Y F+R T +C+ +P + LEP+KD+++ V+ G L E+C+K+L G V L
Sbjct: 236 INNYRGFLRMTGFCKTKIPVEVMAALEPIKDNEEAVKAYGIHLGTEMCKKMLAHG-VKSL 294
Query: 280 HMYTMNLEKASLMILEKLDLLKRSNVGKDVIEPLEPLPWRKSLNPKRRNELVRPIFWQKR 339
H+YT+N+EK++L IL L ++ S + + LPWR+ N R E VRPIFW R
Sbjct: 295 HLYTLNMEKSALAILMNLGMIDESKISRS-------LPWRRPANVFRTKEDVRPIFWANR 347
Query: 340 PYSYVARTSLWNEDEFPNGRFGDSSSPAYGDLDLCGHSLIRQSVKKSIELWSTP-QTLDD 398
P SY++RT W ++FP GR+GDS S +YG L S R KK + W P ++++D
Sbjct: 348 PKSYISRTKGW--EDFPQGRWGDSRSASYGALSDHQFSRPRARDKKLQQEWVVPLKSVED 405
Query: 399 MSNLVTSYLEGKLKCLPWSDVP-LNHEVDDILKHLIDLNKHHIFTINSQPKVNGKPSTDP 457
+ G LK PWS++ L E I + LI +N TINSQP VN + S P
Sbjct: 406 IQEKFKELCLGNLKSSPWSELDGLQPETRIINEQLIKVNSKGFLTINSQPSVNAERSDSP 465
Query: 458 VVGWGPTKGYVYQKEYLEFLLPREKLPLFQEICQEHSDHLTYFAIDSQDNLATNHPEDSR 517
VGWG GYVYQK YLEF +EKL E C+ +TY A++ + +N + +
Sbjct: 466 TVGWGGPVGYVYQKAYLEFFCSKEKLDAVVEKCKA-LPSITYMAVNKGEQWVSNTAQ-AD 523
Query: 518 ANAVTWGVFPGSEILQPTIVEKASFLAWKEEFF-TILKEWKLTFVEHKATESVNLVNHII 576
NAVTWGVFP EI+QPTIV+ ASF WK+E F T + W + E A S NL+ +
Sbjct: 524 VNAVTWGVFPAKEIIQPTIVDPASFNVWKDEAFETWSRSWANLYPE--ADPSRNLLEEVK 581
Query: 577 DNYVLINVVDNDFIS 591
++Y L+++V+ND+I+
Sbjct: 582 NSYYLVSLVENDYIN 596
>At3g59970 [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 592
Score = 421 bits (1082), Expect = e-117
Identities = 238/603 (39%), Positives = 339/603 (55%), Gaps = 31/603 (5%)
Query: 1 MRISQKLQRAHEEREDATFSFEFFVPKTSQGVQNLYERMDRMYQTQPEFIDITWNAGGRL 60
M++ K++ E+ + A FSFEFF PKT GV+NL+ERMDR+ P F DITW AGG
Sbjct: 1 MKVVDKIKSVTEQGQTA-FSFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGST 59
Query: 61 SMLSTDLVATSQGVLGLETCMHLTCTNMPVSQIDDALKKAYDAGCENILALRGDPPINSL 120
+ L+ ++ + Q V+ +ET MHLTCTNMP+ +ID AL+ G +N+LALRGDPP
Sbjct: 60 ADLTLEIASRMQNVICVETMMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHG-- 117
Query: 121 GEEEEWKPCENGFRYAKDLVSYIREQYGDHFDIGVAAYPEGHP---------XXXXXXXX 171
++++ E GF A DLV++IR +YGD+F I VA YPE HP
Sbjct: 118 --QDKFVQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSD 175
Query: 172 XXXXXXXVDCGADFMVTQMFYDVDKFLDWCARVREAXXXXXXXXXXXXXTSYPSFIRRTQ 231
VD GAD +VTQ+FYD D FL + R+ ++Y F+R
Sbjct: 176 LAYLKKKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAG 235
Query: 232 WCQINVPDNFLELLEPVKDDDQLVRDVGTELMVELCQKLLDSGYVNHLHMYTMNLEKASL 291
+C+ +P LEP+KD+D+ V+ G E+C+K+L G + LH+YT+N++K+++
Sbjct: 236 FCKTKIPAELTAALEPIKDNDEAVKAYGIHFATEMCKKILAHG-ITSLHLYTLNVDKSAI 294
Query: 292 MILEKLDLLKRSNVGKDVIEPLEPLPWRKSLNPKRRNELVRPIFWQKRPYSYVARTSLWN 351
IL L L+ S + + LPWR+ N R E VRPIFW RP SY++RT WN
Sbjct: 295 GILMNLGLIDESKISRS-------LPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWN 347
Query: 352 EDEFPNGRFGDSSSPAYGDLDLCGHSLIRQSVKKSIELWSTP-QTLDDMSNLVTSYLEGK 410
+FP+GR+GDS S AY L + + KK + W P ++++D+ G
Sbjct: 348 --DFPHGRWGDSHSAAYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQEKFKELCIGN 405
Query: 411 LKCLPWSDVP-LNHEVDDILKHLIDLNKHHIFTINSQPKVNGKPSTDPVVGWGPTKGYVY 469
LK PWS++ L E I + L +N + TINSQP VN S P +GWG GYVY
Sbjct: 406 LKSSPWSELDGLQPETKIINEQLGKINSNGFLTINSQPSVNAAKSDSPAIGWGGPGGYVY 465
Query: 470 QKEYLEFLLPREKLPLFQEICQEHSDHLTYFAIDSQDNLATNHPEDSRANAVTWGVFPGS 529
QK YLEF ++KL E + +TY A++ +N +N E S NAVTWGVFP
Sbjct: 466 QKAYLEFFCSKDKLDTLVEKSKAFPS-ITYMAVNKSENWVSNTGE-SDVNAVTWGVFPAK 523
Query: 530 EILQPTIVEKASFLAWKEEFFTI-LKEWKLTFVEHKATESVNLVNHIIDNYVLINVVDND 588
E++QPTIV+ ASF WK+E F I + W + E S L+ + ++Y L+++VDN+
Sbjct: 524 EVIQPTIVDPASFKVWKDEAFEIWSRSWANLYPED--DPSRKLLEEVKNSYYLVSLVDNN 581
Query: 589 FIS 591
+I+
Sbjct: 582 YIN 584
>YPL023c [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 657
Score = 364 bits (935), Expect = e-100
Identities = 237/664 (35%), Positives = 336/664 (49%), Gaps = 86/664 (12%)
Query: 11 HEEREDATFSFEFFVPKTSQGVQNLYERMDRMYQTQPEFIDITWNAGGRLSMLSTDLVAT 70
+ R S EFF PKT G +NL ERM RM P FI +TW AGG + + L +
Sbjct: 7 YHARASPFISLEFFPPKTELGTRNLMERMHRMTALDPLFITVTWGAGGTTAEKTLTLASL 66
Query: 71 SQGVLGLETCMHLTCTNMPVSQIDDALKKAYDAGCENILALRGDPPINSLGEEEEW---K 127
+Q L + CMHLTCTN + IDDAL + Y+AG NILALRGDPPI E+W +
Sbjct: 67 AQQTLNIPVCMHLTCTNTEKAIIDDALDRCYNAGIRNILALRGDPPIG-----EDWLDSQ 121
Query: 128 PCENGFRYAKDLVSYIREQYGDHFDIGVAAYPEGHPXXXXX------XXXXXXXXXXVDC 181
E+ F+YA DLV YI++ YGD F +GVAAYPEGH V+
Sbjct: 122 SNESPFKYAVDLVRYIKQSYGDKFCVGVAAYPEGHCEGEAEGHEQDPLKDLVYLKEKVEA 181
Query: 182 GADFMVTQMFYDVDKFLDWCARVREAXXXXX-XXXXXXXXTSYPSFIRRTQWCQINVPDN 240
GADF++TQ+FYDV+KFL + RE SY F R + ++P
Sbjct: 182 GADFVITQLFYDVEKFLTFEMLFRERISQDLPLFPGLMPINSYLLFHRAAKLSHASIPPA 241
Query: 241 FLELLEP-VKDDDQLVRDVGTELMVELCQKLLD--SGYVNHLHMYTMNLEKASLMILEKL 297
L P ++ DD V+ +G ++++EL Q++ SG + H YT+NLEKA I+ +
Sbjct: 242 ILSRFPPEIQSDDNAVKSIGVDILIELIQEIYQRTSGRIKGFHFYTLNLEKAIAQIVSQS 301
Query: 298 DLLKR------SNVGKD----VIEPLEPLPWRKS--------------LNPKRRNE---- 329
+L G+D I +E +P + N KRR
Sbjct: 302 PVLSHIVNESSEEEGEDETSGEIGSIENVPIEDADGDIVLDDSNEETVANRKRRRHSSLD 361
Query: 330 -----LVRPIFWQK---------------------RPYSYVARTSLWNEDEFPNGRFGDS 363
R I +K + + + R + W DEFPNGRFGDS
Sbjct: 362 SAKLIFNRAIVTEKGLRYNNENGSMPSKKALISISKGHGTLGRDATW--DEFPNGRFGDS 419
Query: 364 SSPAYGDLDLCGHSLIRQSVKKSIELWSTPQTLDDMSNLVTSYLEGKLKCLPWSDVPLNH 423
SPAYG++D G S I+ S K++ELW P+T+ D+ ++ YLEG +PWSD+ L+
Sbjct: 420 RSPAYGEIDGYGPS-IKVSKSKALELWGIPKTIGDLKDIFIKYLEGSTDAIPWSDLGLSA 478
Query: 424 EVDDILKHLIDLNKHHIFTINSQPKVNGKPSTDPVVGWGPTKGYVYQKEYLEFLLPREKL 483
E I + LI LN T+ SQP N S+D + GWGP KG +YQK ++E + R++
Sbjct: 479 ETALIQEELIQLNYRGYLTLASQPATNATLSSDKIFGWGPAKGRLYQKAFVEMFIHRQQ- 537
Query: 484 PLFQEICQEHSDH-----LTYFAIDSQDNLATNHPEDSRANAVTWGVFPGSEILQPTIVE 538
++ + DH +Y+A DS + TN S ++ VTWGVFP S + Q TI+E
Sbjct: 538 --WETTLKPKLDHYGRRKFSYYAGDSSGSFETNLDPHS-SSVVTWGVFPNSPVKQTTIIE 594
Query: 539 KASFLAWKEEFFTILKEWKLTFVEHKATESVNLVNHIIDNYVLINVVDNDFISTNDTIYD 598
+ SF AW++E F+I EW F + T + L+ + +Y L+++V +DF T++
Sbjct: 595 EESFKAWRDEAFSIWSEWAKLFPRN--TPANILLRLVHKDYCLVSIVHHDFKETDELWEM 652
Query: 599 LLQQ 602
LL Q
Sbjct: 653 LLDQ 656
>SPAC343.10 [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 641
Score = 342 bits (876), Expect = 1e-93
Identities = 218/630 (34%), Positives = 335/630 (52%), Gaps = 64/630 (10%)
Query: 16 DATFSFEFFVPKTSQGVQNLYERMDRMYQTQ-PEFIDITWNAGGRLSMLSTDLVATSQGV 74
+A +SFE+F PKT+ G++NL R+ RM + P F +TW G + +S L +
Sbjct: 14 NAFYSFEYFPPKTTVGLENLSSRITRMSRNMMPLFSSVTWGTAGSTAEVSIYLAKMLEQH 73
Query: 75 LGLETCMHLTCTNMPVSQIDDALKKAYDAGCENILALRGDPPINSLGEEEEWKPCENGFR 134
+ C+HLTCTN+ + ID AL+ A + G NILALRGDPP+NS + W+ + F
Sbjct: 74 HKIPACLHLTCTNVDKAIIDKALETAKEYGIRNILALRGDPPLNS----DHWEGKVSEFE 129
Query: 135 YAKDLVSYIREQYGDHFDIGVAAYPEGHPXXXXXXXXXX------XXXXXVDCGADFMVT 188
+A DLV YIRE+YGD+F IGVAAYPEGH V+ GADF++T
Sbjct: 130 HAVDLVRYIREKYGDYFCIGVAAYPEGHVDSNVPELSKDPLRDIPFLIEKVEAGADFIIT 189
Query: 189 QMFYDVDKFLDWCARVR----EAXXXXXXXXXXXXXTSYPSFIRRTQWCQINVPDNFLEL 244
Q+FY+ + F+ + VR A SY S R T+ C +VP + ++
Sbjct: 190 QIFYEPEAFIKFENFVRNHSSNALRNIPIIPAIMPIQSYGSLKRMTRLCGCSVPSSLMQR 249
Query: 245 LEPVKDDDQLVRDVGTELMVELCQKLLDS--GYVNHLHMYTMNLEKASLMIL-------- 294
L K DD+ ++++G E +V++ +K++D+ G V+ H T+NLE++ +IL
Sbjct: 250 LNAAKPDDEAIKNIGVEHIVDMIKKIMDNVQGRVHGFHFCTLNLERSVALILKNSGLLTK 309
Query: 295 ---------EKLDLLKRSNVGKDVIEPLE-----PLPWRKSLNPKRRNELV--------- 331
E LL+ + + EP E +P ++ + K N V
Sbjct: 310 RWKQVESEMEDEKLLRTTRKRLSLDEPAELHNQVVVPSQQPVADKSSNLFVTSKQSSVSG 369
Query: 332 -RPIFWQKRPYS------YVARTSLWNEDEFPNGRFGDSSSPAYGDLDLCGHSLIRQSVK 384
+ ++ P+S + R + W D+F NGRFGD SPAYG++D G +L
Sbjct: 370 HKDNLTEEAPFSVSEGSGVLGRQANW--DDFTNGRFGDPRSPAYGEIDGYGPTL-HFPPS 426
Query: 385 KSIELWSTPQTLDDMSNLVTSYLEGKLKCLPWSDVPLNHEVDDILKHLIDLNKHHIFTIN 444
++++LW P D+++L ++ + +PW D P+ E I K+L+ LN + +T+
Sbjct: 427 EALKLWGYPVDESDITSLFQKHIMSDISAIPWIDEPVEVETKTIAKYLLKLNGNSWWTVG 486
Query: 445 SQPKVNGKPSTDPVVGWGPTKGYVYQKEYLEFLLPREKLPLFQEICQEHSD-HLTYFAID 503
SQP VNG PS DPV GWGP G V+QK ++E + + L F I + H + +TY+A +
Sbjct: 487 SQPAVNGAPSADPVFGWGPKGGRVFQKAFVECFVNGKDLKDF--ITKWHDNPQVTYYAGN 544
Query: 504 SQDNLATNHPEDSRANAVTWGVFPGSEILQPTIVEKASFLAWKEEFFTILKEWKLTFVEH 563
++ TN P+D A+AVTWGV+PG EI+Q TI+ + SF AW E F + EW +
Sbjct: 545 NKSEFLTNAPKDG-ASAVTWGVYPGREIIQSTIIAEVSFKAWLSESFQVWGEWANLY--S 601
Query: 564 KATESVNLVNHIIDNYVLINVVDNDFISTN 593
K T S L+ + I++ L+ V+ +DF+ N
Sbjct: 602 KNTPSRKLLENCINDRWLVTVIHHDFMDKN 631
>CE02451 [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 615
Score = 286 bits (731), Expect = 8e-77
Identities = 186/618 (30%), Positives = 302/618 (48%), Gaps = 85/618 (13%)
Query: 3 ISQKLQRAHEEREDATFSFEFFVPKTSQGVQNLYERMDRMYQTQPEFIDITWNAGGRLSM 62
+ ++++R +E++ FS EFF P+ GV N ER++R+ + F+D+TW+ G +
Sbjct: 58 LHERIERLIDEKQQF-FSLEFFPPRFVNGVPNFLERVERLSEGGSVFVDMTWHMGSDPAN 116
Query: 63 L-----STDLVATSQGVLGLETCMHLTCTNMPVSQIDDALKKAYDAGCENILALRGD-PP 116
+ S+ + A+ G++T +H+TC + L++A G +ILALRGD PP
Sbjct: 117 VDKVTSSSSIAASMLDYCGVDTMLHMTCVQYNKADTLKHLEQAKAMGLRSILALRGDLPP 176
Query: 117 INSLGEEEEWKPCENGFRYAKDLVSYIREQYGDHFDIGVAAYPEGHPXXXXXXXXXXXXX 176
L + +++ A D++ +IRE+YG++F IG A YP GHP
Sbjct: 177 GTELEDTHQFR--------ALDMIRWIREEYGNYFSIGCAGYPLGHPQAPSYKADLMYLK 228
Query: 177 XXVDCGADFMVTQMFYDVDKFLDWCARVREAXXXXXXXXXXXXXTSYPSFIRRTQWCQIN 236
D GA+F++TQ+F++ + F + RE Y S R + Q+
Sbjct: 229 AKCDAGANFVITQLFFEAETFEKFVRDCREIGITQPIIPGIMPIMGYESIKRIAKLSQLE 288
Query: 237 VPDNFLELLEPVKDDDQLVRDVGTELMVELCQKLLDSGYVNHLHMYTMNLEKASLMILEK 296
+P + L+ LEP+K DD V+ GTE +E+C++LLD+G +H+YTMN E + IL+
Sbjct: 289 IPQHILDDLEPIKHDDDAVQKYGTERCIEMCRRLLDNGTAPSIHLYTMNREGSIREILKS 348
Query: 297 LDLLKRSNVGKDVIEPLEPLPWR-KSLNPKRRNELVRPIFWQKRPYSYVARTSLWNEDEF 355
L L K +E PW+ +S +P R
Sbjct: 349 LGLWK--------LEGDRVFPWKNRSQHPIR----------------------------- 371
Query: 356 PNGRFGDSSSPAYGDLDLCGHSLIRQSVKKSIELWSTPQTLDDMSNLVTSYLEG------ 409
C S +R + + + ++ +D+ + +Y+
Sbjct: 372 ------------------CLESTVRNADDRLAMFGANIESFEDVKRVFINYITQAPNADG 413
Query: 410 -KLKCLPWSDVP--LNHEVDDILKHLIDLNKHHIFTINSQPKVNGKPSTDPVVGWGPTKG 466
K+ LPW++ + E I + L+ N++ I T+NSQP VNG PSTDP+VGWG G
Sbjct: 414 VKVTVLPWTEAETGVQPETSLISEQLVWCNENGILTVNSQPSVNGAPSTDPLVGWGKPGG 473
Query: 467 YVYQKEYLE-FLLPREKLPLFQEICQEHSDHLTYFAIDSQDNLATNHPEDSRANAVTWGV 525
Y YQK YLE F+ L Q I +E + Y AI+ + E++ AVTWGV
Sbjct: 474 YCYQKAYLECFMTAELSDKLIQIIEREFPVRVNYHAINKDSTFDKTNSEETTPIAVTWGV 533
Query: 526 FPGSEILQPTIVEKASFLAWKEEFFTI-LKEWKLTFVEHKATESVNLVNHIIDNYVLINV 584
FPGSEI QPT+V+ SF AW++E + + + +W + K ++S ++ + D + L+ +
Sbjct: 534 FPGSEIAQPTVVDPLSFRAWRDEAYQMWMAQWGDFY--PKESKSYGVIKAVHDEFRLVTL 591
Query: 585 VDNDFISTNDTIYDLLQQ 602
VDNDF ++D+LQ+
Sbjct: 592 VDNDF-QKPSVLFDVLQK 608
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.137 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,006,363
Number of Sequences: 60738
Number of extensions: 1705253
Number of successful extensions: 3930
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3858
Number of HSP's gapped (non-prelim): 11
length of query: 604
length of database: 30,389,216
effective HSP length: 112
effective length of query: 492
effective length of database: 23,586,560
effective search space: 11604587520
effective search space used: 11604587520
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)