ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI2540 good S KOG0997 Function unknown Uncharacterized conserved protein Sand
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI2540 900685 899102 -528
(528 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGL124c [S] KOG0997 Uncharacterized conserved protein Sand 234 2e-61
7295663 [S] KOG0997 Uncharacterized conserved protein Sand 129 1e-29
SPAC1D4.03c [S] KOG0997 Uncharacterized conserved protein Sand 105 2e-22
Hs14150159 [S] KOG0997 Uncharacterized conserved protein Sand 104 3e-22
Hs7662354 [S] KOG0997 Uncharacterized conserved protein Sand 85 3e-16
CE27161 [S] KOG0997 Uncharacterized conserved protein Sand 69 2e-11
>YGL124c [S] KOG0997 Uncharacterized conserved protein Sand
Length = 644
Score = 234 bits (598), Expect = 2e-61
Identities = 171/571 (29%), Positives = 268/571 (45%), Gaps = 78/571 (13%)
Query: 24 TNHLSARGGLFYQDIDTGTTASIKAKNDPNLLAATYDHSISADTDLNIDLQSLITSELNS 83
T L A+ L D+ + +A N P Y + ++ D+ L I S S
Sbjct: 86 TKKLQAKNNLLSADLAKSNDDTTRALNCPKKDFGPY---LDSENDIRSRLAESIYSMETS 142
Query: 84 LYPLTLNQE--TSNKSANTTKFI---------DERGGKDKHFFVFTSAGKLVFSQWENEN 132
+ L + SN+ N KF + + DK+FF+FTSAGK ++ +
Sbjct: 143 IRGSELQRRPYVSNEIPNVFKFSKFNSNCKLNESQTLCDKNFFIFTSAGKPIYCMHGKDE 202
Query: 133 HAMGLTGIIHTVMNYFNINDNTAMRQFTMYGKDGIMTRFVFLDKNHIKLMVQCNNNYEST 192
M TG+++TV++YF +N + ++ + R FLDK+ I LM Q ES+
Sbjct: 203 QIMSYTGLVNTVISYFQVNGPSELKTISTLTSG---KRLTFLDKSPILLMAQSERG-ESS 258
Query: 193 AQLQQQLDLVYSYIISSVSQRNLNKLMLKRSNFDLQHYLTDLDQQLLKSLCESLATQPKL 252
+L QLD +YSYI+SS+S+R L +L KR NFDL++YL D + L +C + +
Sbjct: 259 NELLNQLDFLYSYILSSLSERQLLRLFSKRENFDLRNYLESTDFENLDEICSLICNRMFP 318
Query: 253 TWFANSLECLPMSPKKRNAINSILSTTYLDFNISNHNGQILYTLVTSLDMRLISILRPSN 312
NSL+CLP + R + +++ L+ G +LY L+ + +L +LRP
Sbjct: 319 DLLLNSLQCLPFNHSSRLKLQNVV-LQQLEKRQDIPRGTLLYGLIIAPQNKLCCVLRPRG 377
Query: 313 HTLHTMDLQILFEVVRAQLTDLLLDKVLWFPICFQKFNDNGFLYALIKVLPNNT------ 366
HTLHT DL +LF ++ Q +L + LW PICF KFN +GFLY IK LPN+T
Sbjct: 378 HTLHTTDLHLLFCLISHQFQNLDETQELWVPICFPKFNSSGFLYCYIKFLPNDTHSNEKS 437
Query: 367 IMMVISSQKNAFDILNEF-----VRRIEDKMIDD-NTCNNLELQLLDWHKKFPYINHFIY 420
+++IS+QK+AF L F ++ E+K++ NT +L + P ++HFIY
Sbjct: 438 ALVLISAQKDAFFSLKSFSDELIIKLEEEKLLKKINTSKGFKLSDI----PAPMVHHFIY 493
Query: 421 KMKRTVQIYTPSQPTN---------------EMLQFYYHLKNLCEDDKGTNLNKSSVAML 465
K K+ VQ P N ++ +Y L D G L++S + +
Sbjct: 494 KSKQNVQYVMPHFEVNSNIALDSSQGLEYELKLKTYYQQLHGTVVRDNGNLLSRSMLNFV 553
Query: 466 KWKNDATPGL----------------------------LSGVYWATEKFELYILLNDINL 497
+W + L + G+ W T FELY++ N+ +
Sbjct: 554 RWSSKDNEDLAMDETQMDFSELDEYIIGNSSFKQESVNMVGMAWVTPTFELYLIGNNGIV 613
Query: 498 SNQSILKSAKLLIQHIREIESYLFISRGVTF 528
+ + KSA+ + ++ ES LFIS G F
Sbjct: 614 DKRVLFKSARKVANWCQKHESRLFISDGAVF 644
>7295663 [S] KOG0997 Uncharacterized conserved protein Sand
Length = 528
Score = 129 bits (323), Expect = 1e-29
Identities = 112/444 (25%), Positives = 210/444 (47%), Gaps = 53/444 (11%)
Query: 109 GKDKHFFVFTSAGKLVFSQWENENHAMGLTGIIHTVMNYFNINDNTAMRQFTMYGKDGIM 168
G+ KH F+ + AGK +FS NE+ L G+I ++++ + + T GI
Sbjct: 114 GQKKHIFILSEAGKPIFSLHGNEDKLATLFGVIQALVSFVQMGQDA----ITSIHAGGI- 168
Query: 169 TRFVFLDKNHIKLMVQCNNNYESTAQLQQQLDLVYSYIISSVSQRNLNKLMLKRSNFDLQ 228
+F F+ ++ + L+ +N S QLQ QL VY+ I+S ++ ++ K+ +R NFDL+
Sbjct: 169 -KFAFMQRSSLILVAASRSNM-SVQQLQLQLGDVYNQILSILTYSHMTKIFERRKNFDLR 226
Query: 229 HYLTDLDQQLLKSLCESLATQPKL-----TWFANSLECLPMSPKKRNAINSILSTTYLDF 283
L+ ++L +L + ++ K+ T+ NS+ P+ R+ I S + +
Sbjct: 227 RLLSG-SERLFYNLLANDSSSAKVSNNIFTFLTNSIRVFPLPTTIRSQITSAIQS----- 280
Query: 284 NISNHNGQILYTLVTSLDMRLISILRPSNHTLHTMDLQILFEVVRAQLTDLLLDKVLWFP 343
N S + L+ + +LI+++R +++H DL+++F +V + ++ W P
Sbjct: 281 NCSKIKNLVFAVLIA--NNKLIALVRMKKYSIHPADLRLIFNLV--ECSESFKSSENWSP 336
Query: 344 ICFQKFNDNGFLYALIKVLPNN--TIMMVISSQKNAFDILNEFVRRIEDKM--------I 393
IC KF+ NG+L+A + L ++ ++++S ++AF L E +I +K+ I
Sbjct: 337 ICLPKFDMNGYLHAHVSYLADDCQACLLLLSVDRDAFFTLAEAKAKITEKLRKSHCLEAI 396
Query: 394 DDNTCNNLELQLLDWHKKFPYINHFIYKMKRTVQIYTPSQPTNEMLQFYYHLKNLCEDDK 453
++ +L P + HF+YK K T Q+ P ML+ Y K+L E ++
Sbjct: 397 NEELQQPFNAKLYQQVVGIPELRHFLYKPKSTAQLLCP------MLRHPY--KSLTELER 448
Query: 454 GTNL---------NKSSVAMLKWKNDATPGLLSGVYWATEKFELYILLNDINLSNQSILK 504
+ N S L ++ +L+ WAT +ELY + + + +++K
Sbjct: 449 LEAIYCDLLHRIHNSSRPLKLIYEMKEREVVLA---WATGTYELYAIFEPV-VDKATVIK 504
Query: 505 SAKLLIQHIREIESYLFISRGVTF 528
LI+ I + FI TF
Sbjct: 505 YVDKLIKWIEKEYDVYFIRNHATF 528
>SPAC1D4.03c [S] KOG0997 Uncharacterized conserved protein Sand
Length = 513
Score = 105 bits (261), Expect = 2e-22
Identities = 101/461 (21%), Positives = 204/461 (43%), Gaps = 48/461 (10%)
Query: 89 LNQETSNKSANTTKFIDE-----------RGGKDKHFFVFTSAGKLVFSQWENENHAMGL 137
LN TS S++ +DE + + + +F+S+GK VFS +++
Sbjct: 80 LNVSTSVSSSSNNTAVDEIIKLLSLISFDLAKQKRTYLIFSSSGKPVFSNIVDDSIEPST 139
Query: 138 TGIIHTVMNYFNINDNTAMRQFTMYGKDGIMTRFVFLDKNHIKLMVQCNNNYESTAQLQQ 197
G + +++ F ++ + F+ + V L KN + L+ + S A L
Sbjct: 140 VGALQAIISSFEVSKEE-LTSFSTFSNV-----IVVLSKNPLYLVGVSPSTTLSAAYLLS 193
Query: 198 QLDLVYSYIISSVSQRNLNKLMLKRSNFDLQHYLTDLDQQLLKSLCESLATQPKLTWFAN 257
+L+L+Y I++ V+ + + + R NFDL+ L ++Q LK LC+ L +L N
Sbjct: 194 ELNLLYCQILTGVTAKAMQLTLNSRPNFDLRR-LIGSNEQFLKELCDQL-NDYELVPTLN 251
Query: 258 SLECLPMSPKKRNAINSILSTTYLDFNISNHNGQILYTLVTSLDMRLISILRPSNHTLHT 317
++ LP+ R+ ++ +L + +L+T + ++ RL+ +++ LH
Sbjct: 252 AISPLPLRSSFRDQLSQLL--------LRETPKSLLFTFI-AIRGRLVCMVKAKKLLLHA 302
Query: 318 MDLQILF-EVVRAQLTDLLLDKVLWFPICFQKFNDNGFLYALIKVLPNNTIMMVISSQKN 376
DL +LF + R Q + ++ W P+CF N + ++Y L +T++++ SS+
Sbjct: 303 NDLYLLFLSLFRTQSFNDSMEH--WVPVCFPTLNPDAYIYIYSYFLCKDTVLIMGSSESG 360
Query: 377 AFDILNEFVRRIEDKMIDDN------TCNNLELQLLDWHKKFPYINHFIYKMKRTVQIYT 430
F + ++ ++ D C ++ + + P I+H+++ K+ Q YT
Sbjct: 361 VFFEMQSVKCKVAQEIQDHGWLKKLIYCEEMD-RTTPRNPGSPCISHYLFYSKKYSQFYT 419
Query: 431 PS---QPTNEMLQFYYHLKNLCEDDKGTNLNKSSVAMLKWKNDATPGLLSGVYWATEKFE 487
P N + Y + D N S+ M ++ L W+T F+
Sbjct: 420 PGYSFSTPNFNTRTLYAIYASLHDQAFHKKNSFSINMTVHES------LLLFTWSTASFD 473
Query: 488 LYILLNDINLSNQSILKSAKLLIQHIREIESYLFISRGVTF 528
+ + N S+Q ++ + +++ IR E+ LFI ++F
Sbjct: 474 FHCIAN-ATTSSQLLIANVNKILRWIRREENRLFIQTNLSF 513
>Hs14150159 [S] KOG0997 Uncharacterized conserved protein Sand
Length = 555
Score = 104 bits (260), Expect = 3e-22
Identities = 94/432 (21%), Positives = 200/432 (45%), Gaps = 47/432 (10%)
Query: 112 KHFFVFTSAGKLVFSQWENENHAMGLTGIIHTVMNYFNINDNTAMRQFTMYGKDGIMTRF 171
KH FV + AGK V+S++ +E G++ ++++ + N A+R G +
Sbjct: 156 KHVFVLSEAGKPVYSRYGSEEALSSTMGVMVALVSFLEADKN-AIRSIHADGY-----KV 209
Query: 172 VFLDKNHIKLMVQCNNNYESTAQLQQQLDLVYSYIISSVSQRNLNKLMLKRSNFDLQHYL 231
VF+ ++ + L+ +S +L Q+L +Y I+S ++ L+ + ++ N+DL+ L
Sbjct: 210 VFVRRSPLVLVAVARTR-QSAQELAQELLYIYYQILSLLTGAQLSHIFQQKQNYDLRRLL 268
Query: 232 TDLDQQLLKSLCESLATQPKLTWFANSLECLPMSPKKRNAINSILSTTYLDFNISNHNGQ 291
+ +++ +L + +A P ++ + CLP++ R+ +++ L
Sbjct: 269 SG-SERITDNLLQLMARDP--SFLMGAARCLPLAAAVRDTVSASLQQA--------RARS 317
Query: 292 ILYTLVTSLDMRLISILRPSNHTLHTMDLQILFEVVRAQLTDLLLDKVLWFPICFQKFND 351
++++++ + + +L++++R + LH +DL +LF ++ + + + W P+C KFN
Sbjct: 318 LVFSILLARN-QLVALVRRKDQFLHPIDLHLLFNLISSSSS--FREGEAWTPVCLPKFNA 374
Query: 352 NGFLYALIKVLPNNT--IMMVISSQKNAFDILNEFVRRIEDKMIDDNTCNNLELQLLDWH 409
GF +A I L +T ++++S+ + F +++ RR ++++ L L +
Sbjct: 375 AGFFHAHISYLEPDTDLCLLLVSTDREDFFAVSDCRRRFQERLRKRGAHLALREALRTPY 434
Query: 410 KK-----FPYINHFIYKMKRTVQI--------YTPSQPTNEMLQFYYHLKNLCEDDKGTN 456
P + HF+YK K + YT + +L Y +L +
Sbjct: 435 YSVAQVGIPDLRHFLYKSKSSGLFTSPEIEAPYTSEEEQERLLGLYQYLHSRAH------ 488
Query: 457 LNKSSVAMLKWKNDATPGLLSGVYWATEKFELYILLNDINLSNQSILKSAKLLIQHIREI 516
N S + LL+ W T FELY+ + + + S + + L++ IR+
Sbjct: 489 -NASRPLKTIYYTGPNENLLA---WVTGAFELYMCYSPLG-TKASAVSAIHKLMRWIRKE 543
Query: 517 ESYLFISRGVTF 528
E LFI +T+
Sbjct: 544 EDRLFILTPLTY 555
>Hs7662354 [S] KOG0997 Uncharacterized conserved protein Sand
Length = 547
Score = 84.7 bits (208), Expect = 3e-16
Identities = 105/467 (22%), Positives = 204/467 (43%), Gaps = 63/467 (13%)
Query: 88 TLNQETSNKSANTTKFIDERGGKDKHFFVFTSAGKLVFSQWENENHAMGLTGIIHTVMNY 147
T + E+S+ +E + KH FV + AGK ++S++ + G++ ++++
Sbjct: 86 TCSPESSSGGQGGDPSDEEWRSQRKHVFVLSEAGKPIYSRYGSVEALSATMGVMTALVSF 145
Query: 148 FNINDNTAMRQFTMYGKDGIMTRFVFLDKNHIKLMVQCNNNYESTAQLQQQLDLVYSYII 207
+ A+R +Y +D + VFL + + L+V + +S AQL+ +L V++ I+
Sbjct: 146 VQ-SAGDAIR--AIYAEDH---KLVFLQQGPL-LLVAMSRTSQSAAQLRGELLAVHAQIV 198
Query: 208 SSVSQRNLNKLMLKRSNFDLQHYLTDLDQQLLKSLCESLATQPKLTWFANSLECLPMSPK 267
S++++ ++ ++ + N+DL+ L ++ L L +S+ P ++ C+P++
Sbjct: 199 STLTRASVARIFAHKQNYDLRRLLAG-SERTLDRLLDSMEQDPG-ALLLGAVRCVPLARP 256
Query: 268 KRNAINSILSTTYLDFNISNHNGQILYTLVTSLDMRLISILRPSN----HTLHTMDLQIL 323
R+A+ ++L L V ++ RLI+ + N L DLQ+L
Sbjct: 257 LRDALGALLRRC---------TAPGLALSVLAVGGRLITAAQERNVLAECRLDPADLQLL 307
Query: 324 FEVVRAQLTDLLLDKVLWFPICFQKFNDNGFLYALIKVLPNNTI-MMVISSQKNAFDILN 382
+ V A W P+C +FN +GF YA + L + ++++ +Q+ AF +
Sbjct: 308 LDWVGA---PAFAAGEAWAPVCLPRFNPDGFFYAYVARLDAMPVCLLLLGTQREAFHAMA 364
Query: 383 EFVRRIEDKMI---------DDNTCNNLELQLLDWHK----KFPYINHFIYK-------M 422
R +ED M + + +N + P + HF+YK
Sbjct: 365 ACRRLVEDGMHALGAMRALGEAASFSNASSASAPAYSVQAVGAPGLRHFLYKPLDIPDHH 424
Query: 423 KRTVQIYTPS-------QPTNEMLQFYYHLKNLCEDDKGTNLNKSSVAMLKWKNDATPGL 475
++ Q +P + + L YH + L+ +S + + A
Sbjct: 425 RQLPQFTSPELEAPYSREEERQRLSDLYHRLH-------ARLHSTSRPLRLIYHVAEKET 477
Query: 476 LSGVYWATEKFELYILLNDINLSNQSILKSAKLLIQHIREIESYLFI 522
L + W T KFELY L+ + +IL KLL + +++ E LFI
Sbjct: 478 L--LAWVTSKFELYTCLSPLVTKAGAILVVTKLL-RWVKKEEDRLFI 521
>CE27161 [S] KOG0997 Uncharacterized conserved protein Sand
Length = 479
Score = 68.9 bits (167), Expect = 2e-11
Identities = 53/206 (25%), Positives = 101/206 (48%), Gaps = 16/206 (7%)
Query: 194 QLQQQLDLVYSYIISSVSQRNLNKLMLKRS-NFDLQHYLTDLDQQLLKSLCESLATQPKL 252
QL QQL++++ I S +S+ L + K+ N+DL+ L D+ L+ S S P +
Sbjct: 142 QLDQQLEVLFEQICSILSKSQLENVYKKKGDNYDLRKLLRGTDR-LIDSSISSWRASP-I 199
Query: 253 TWFANSLECLPMSPKKRNAINSILSTTYLDFNISNHNGQILYTLVTSLDMRLISILRPSN 312
+S+ +PM+P R +++ +++ + +G + ++ ++ +++R
Sbjct: 200 NLVDSSISAIPMNPSDREFLSTTMASC---LGAAKLDGALFGIMIAR--RQIAAMVRFKK 254
Query: 313 HTLHTMDLQILFEVVRAQLTDLLLDKVLWFPICFQKFNDNGFLYALI------KVLPNNT 366
+ +H DL I+ +V L D W PIC +FND GF YA I K
Sbjct: 255 YMIHPRDLNIVINLVSDNT--LQTDSQNWVPICLPRFNDTGFFYAYISYPWCNKEQDIPV 312
Query: 367 IMMVISSQKNAFDILNEFVRRIEDKM 392
++++S +++ FD L E ++I K+
Sbjct: 313 CIVLLSVKRDHFDGLKEVRQQIVTKL 338
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.134 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,241,225
Number of Sequences: 60738
Number of extensions: 1267399
Number of successful extensions: 3327
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 3306
Number of HSP's gapped (non-prelim): 9
length of query: 528
length of database: 30,389,216
effective HSP length: 111
effective length of query: 417
effective length of database: 23,647,298
effective search space: 9860923266
effective search space used: 9860923266
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)