ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI2751 good A KOG1975 RNA processing and modification mRNA cap methyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI2751 969804 971081 426
(426 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBR236c [A] KOG1975 mRNA cap methyltransferase 600 e-171
SPCC330.10 [A] KOG1975 mRNA cap methyltransferase 224 2e-58
At3g20650 [A] KOG1975 mRNA cap methyltransferase 191 1e-48
Hs4506567 [A] KOG1975 mRNA cap methyltransferase 160 4e-39
ECU10g0380 [A] KOG1975 mRNA cap methyltransferase 140 5e-33
CE08368 [A] KOG1975 mRNA cap methyltransferase 137 3e-32
7298196 [A] KOG1975 mRNA cap methyltransferase 102 8e-22
>YBR236c [A] KOG1975 mRNA cap methyltransferase
Length = 436
Score = 600 bits (1547), Expect = e-171
Identities = 292/425 (68%), Positives = 338/425 (78%), Gaps = 3/425 (0%)
Query: 5 PEKPVWMSQQQYEEQYGSLLKLKESEKCNHEDKQLALQPKES--SIGYSTQERIAKLSVP 62
PEKP+WMSQ+ Y+ QYGS+ + S + K A P + S+ I V
Sbjct: 5 PEKPIWMSQEDYDRQYGSITGDESSTVSKKDSKVTANAPGDGNGSLPVLQSSSILTSKVS 64
Query: 63 EVQ-DDDKNSKVRNRKHQRFDXXXXXXXXRLQKTIEEQIKHHDIEMTANRVVNVDHVVRQ 121
++ + + K++ R+H+R+D R QK EEQ+K H+IEMTANR +NVD +VR+
Sbjct: 65 DLPIEAESGFKIQKRRHERYDQEERLRKQRAQKLREEQLKRHEIEMTANRSINVDQIVRE 124
Query: 122 HYNERTFLSKKHNRNYSPIIKLRNFNNAIKYILIDKFTRAGDVVLELACGKGGDLRKYGA 181
HYNERT ++ + RN SPIIKLRNFNNAIKY+LIDK+T+ GDVVLEL CGKGGDLRKYGA
Sbjct: 125 HYNERTIIANRAKRNLSPIIKLRNFNNAIKYMLIDKYTKPGDVVLELGCGKGGDLRKYGA 184
Query: 182 AGISQFIGIDISNASITEALKRYHSMKNLEYQVILITGDCFGESLGVAVESFPECRFPCD 241
AGISQFIGIDISNASI EA KRY SM+NL+YQV+LITGDCFGESLGVAVE FP+CRFPCD
Sbjct: 185 AGISQFIGIDISNASIQEAHKRYRSMRNLDYQVVLITGDCFGESLGVAVEPFPDCRFPCD 244
Query: 242 IVSCQFALHYAFETEEKARRMLLNVVKSLKIGGYFFGTIPDSEFIRYKMNKIPESVEKPS 301
IVS QF LHYAFETEEKARR LLNV KSLKIGG+FFGTIPDSEFIRYK+NK P+ VEKPS
Sbjct: 245 IVSTQFCLHYAFETEEKARRALLNVAKSLKIGGHFFGTIPDSEFIRYKLNKFPKEVEKPS 304
Query: 302 WGNSIYKVTFSNNEYQKNGNEFPSPFGQMYTFWLEDAIDNVPEYVIPFESFRSLADEYGM 361
WGNSIYKVTF NN YQKN EF SP+GQMYT+WLEDAIDNVPEYV+PFE+ RSLADEYG+
Sbjct: 305 WGNSIYKVTFENNSYQKNDYEFTSPYGQMYTYWLEDAIDNVPEYVVPFETLRSLADEYGL 364
Query: 362 ELELQKGFNEFFVEEIPNWVNRFSPKMREGLKRSDGRYGVEGVEKEPAAYFYTTFAFRKV 421
EL Q FN+FFV+EIP W+ RFSPKMREGL+RSDGRYGVEG EKE A+YFYT FAFRKV
Sbjct: 365 ELVSQMPFNKFFVQEIPKWIERFSPKMREGLQRSDGRYGVEGDEKEAASYFYTMFAFRKV 424
Query: 422 RDYQE 426
+ Y E
Sbjct: 425 KQYIE 429
>SPCC330.10 [A] KOG1975 mRNA cap methyltransferase
Length = 389
Score = 224 bits (570), Expect = 2e-58
Identities = 141/312 (45%), Positives = 185/312 (59%), Gaps = 19/312 (6%)
Query: 114 NVDHVVRQHYNERTFLSKKHNRNYSPIIKLRNFNNAIKYILIDKFT-RAGD---VVLELA 169
N VVR HYN R L K R +SPII+L+ FNN IK +LI KF A D +VL++
Sbjct: 89 NAQDVVRSHYNARPDLGYK-KRQFSPIIQLKRFNNWIKSVLIQKFAPHASDYPILVLDMG 147
Query: 170 CGKGGDLRKYGAAGISQFIGIDISNASITEALKRYHSMKNLEYQVILITGDCFGESLGVA 229
CGKGGDL K+ AGI +IGIDI+ S+ +A KRY M + + + GDCF S+
Sbjct: 148 CGKGGDLIKWDKAGIDGYIGIDIAEVSVNQAKKRYREM-HASFDALFYAGDCFSSSIN-- 204
Query: 230 VESFPECRFPCDIVSCQFALHYAFETEEKARRMLLNVVKSLKIGGYFFGTIPDSEFIRYK 289
E P + D+VS QF +HYAFE+EEK R +L NV K L GG GTIP+S+ I K
Sbjct: 205 -ELLPPDQRKFDVVSLQFCMHYAFESEEKVRVLLGNVSKCLPRGGVMIGTIPNSDVI-VK 262
Query: 290 MNKIPESVEKPSWGNSIYKVTFSNNEYQKNGNEFPSPFGQMYTFWLEDAIDNVPEYVIPF 349
K+ + EK WGN IYKV F + + F P+G Y F+LEDA+ +VPEYV+PF
Sbjct: 263 HIKMLKPGEK-EWGNDIYKVRFPESPPRS----FRPPYGIQYYFYLEDAVTDVPEYVVPF 317
Query: 350 ESFRSLADEYGMELELQKGFNEFFVEEIPNWVNRFSPKM-REGLKRSDGRYGVEGVEKEP 408
E+FR++A+ Y +EL K F + EE + + P M R + ++G G+ G EKE
Sbjct: 318 EAFRAVAEGYNLELIWVKPFLDILNEEKNS--ETYGPLMDRMKVVDNEGHRGIGGQEKE- 374
Query: 409 AAYFYTTFAFRK 420
AA FY FAF K
Sbjct: 375 AAGFYLAFAFEK 386
>At3g20650 [A] KOG1975 mRNA cap methyltransferase
Length = 362
Score = 191 bits (486), Expect = 1e-48
Identities = 126/318 (39%), Positives = 168/318 (52%), Gaps = 22/318 (6%)
Query: 114 NVDHVVRQHYNERTFLSKKHNRNYSPIIKLRNFNNAIKYILIDKFTRAGDVVLELACGKG 173
N V HY+ RT + + R SPII L+ NN IK +LI + R D VL+LACGKG
Sbjct: 38 NFARKVADHYSRRTNQTLEE-REASPIIHLKKLNNWIKSVLIQLYARPDDAVLDLACGKG 96
Query: 174 GDLRKYGAAGISQFIGIDISNASITEALKRYHS-------MKNLEYQVILITGDCFGESL 226
GDL K+ A I ++GIDI+ SI + RY+ K + L+ GDCF
Sbjct: 97 GDLIKWDKARIGYYVGIDIAEGSIEDCRTRYNGDADHHQRRKKFSFPSRLLCGDCF---- 152
Query: 227 GVAVESFPECRFPCDIVSCQFALHYAFETEEKARRMLLNVVKSLKIGGYFFGTIPDSEFI 286
V ++ E P DI SCQFA+HY++ TE +ARR L NV L+ GG F GT+PD+ I
Sbjct: 153 EVELDKILEEDAPFDICSCQFAMHYSWTTEARARRALANVSALLRPGGVFIGTMPDANVI 212
Query: 287 RYKMNKIPESVEKPSWGNSIYKVTFSNNEYQKNGNEFPSPFGQMYTFWLEDAIDNVPEYV 346
K+ + E GNS+Y + F EY + + SPFG Y F LEDA+D PE++
Sbjct: 213 IKKLRE----AEGLEIGNSVYWIRF-GEEYSQKKFKSSSPFGIEYVFHLEDAVD-CPEWI 266
Query: 347 IPFESFRSLADEYGMELELQKGFNEFFVEEIPNWVNRFSPKMREGLKRSDGR--YGVEGV 404
+PF F+SLA+EY +EL K +EF E + F MR DG
Sbjct: 267 VPFNVFKSLAEEYDLELVFVKNSHEFVHEYMKK--PEFVELMRRLGALGDGSNDQSTLSA 324
Query: 405 EKEPAAYFYTTFAFRKVR 422
++ AAY Y +F RK R
Sbjct: 325 DEWEAAYLYLSFVLRKGR 342
>Hs4506567 [A] KOG1975 mRNA cap methyltransferase
Length = 476
Score = 160 bits (404), Expect = 4e-39
Identities = 126/373 (33%), Positives = 184/373 (48%), Gaps = 39/373 (10%)
Query: 41 LQPKESSIGYSTQERIAKLSVPEVQDDDK-------NSKVRNRKHQRFDXXXXXXXXRLQ 93
L + SS G T + KL PE+ ++K NSK R R+ + Q
Sbjct: 66 LVKESSSCGKDTPSKKRKLD-PEIVPEEKDCGDAEGNSKKRKRETEDVPKDKSSTGDGTQ 124
Query: 94 KTIEEQIKHHDI-EMTANRVVNVDHVVRQHYNERTFLSKKHNRNYSPIIKLRNFNNAIKY 152
+ +I D+ E N V HYNE + + R+ S I LRNFNN +K
Sbjct: 125 N--KRKIALEDVPEKQKNLEEGHSSTVAAHYNELQEVGLE-KRSQSRIFYLRNFNNWMKS 181
Query: 153 ILIDKFT-------RAGDVVLELACGKGGDLRKYGAAGISQFIGIDISNASITEALKRYH 205
+LI +F + VL+L CGKGGDL K+ I++ + DI++ S+ + +RY
Sbjct: 182 VLIGEFLEKVRQKKKRDITVLDLGCGKGGDLLKWKKGRINKLVCTDIADVSVKQCQQRYE 241
Query: 206 SMKNLE-----YQVILITGDCFGESLGVAVESFPECRFPCDIVSCQFALHYAFETEEKAR 260
MKN + IT D E L ++ F + + DI SCQF HY+FE+ E+A
Sbjct: 242 DMKNRRDSEYIFSAEFITADSSKELL---IDKFRDPQMCFDICSCQFVCHYSFESYEQAD 298
Query: 261 RMLLNVVKSLKIGGYFFGTIPDSEFIRYKMNKIPESVEKPSWGNSIYKVTFSNNEYQKNG 320
ML N + L GGYF GT P+S +++ + E+ E S+GN IY V F QK G
Sbjct: 299 MMLRNACERLSPGGYFIGTTPNS----FELIRRLEASETESFGNEIYTVKF-----QKKG 349
Query: 321 NEFPSPFGQMYTFWLEDAIDNVPEYVIPFESFRSLADEYGMELELQKGFNEFFVEEIPNW 380
++P FG Y F LE +D VPE+++ F +A +Y M+L +K F EF+ E+I N
Sbjct: 350 -DYPL-FGCKYDFNLEGVVD-VPEFLVYFPLLNEMAKKYNMKLVYKKTFLEFYEEKIKNN 406
Query: 381 VNRFSPKMREGLK 393
N+ K + L+
Sbjct: 407 ENKMLLKRMQALE 419
>ECU10g0380 [A] KOG1975 mRNA cap methyltransferase
Length = 298
Score = 140 bits (352), Expect = 5e-33
Identities = 88/260 (33%), Positives = 130/260 (49%), Gaps = 27/260 (10%)
Query: 119 VRQHYNERTFLSKKHNRNYSPIIKLRNFNNAIKYILIDKFTRAGDVVLELACGKGGDLRK 178
+R+HYN ++ +R S I +RN NN IK LI +T+ GD VL+L CGKGGDL K
Sbjct: 23 IREHYNSIRERGRE-SRQRSKTINIRNANNFIKACLIRLYTKRGDSVLDLGCGKGGDLLK 81
Query: 179 YGAAGISQFIGIDISNASITEALKRYHSMKNLEYQVILITGDCFGESLGVAVESFPECRF 238
Y AGI ++ G+DI+ SI +A R +MK ++V D +G + + E
Sbjct: 82 YERAGIGEYYGVDIAEVSINDARVRARNMKR-RFKVFFRAQDSYGRHMDLGKE------- 133
Query: 239 PCDIVSCQFALHYAFETEEKARRMLLNVVKSLKIGGYFFGTIPDSEFI--RYKMNKIPES 296
D++S QF+ HYAF T E N+ + L+ GGYF T+P + I RYK ++
Sbjct: 134 -FDVISSQFSFHYAFSTSESLDIAQRNIARHLRPGGYFIMTVPSRDVILERYKQGRM--- 189
Query: 297 VEKPSWGNSIYKVTFSNNEYQKNGNEFPSPFGQMYTFWLEDAIDNVPEYVIPFESFRSLA 356
N YK+ E + P + Y F L D+++N EY + F
Sbjct: 190 ------SNDFYKIELEKME------DVPMESVREYRFTLLDSVNNCIEYFVDFTRMVDGF 237
Query: 357 DEYGMELELQKGFNEFFVEE 376
G+ L +KGF +F+ +E
Sbjct: 238 KRLGLSLVERKGFIDFYEDE 257
>CE08368 [A] KOG1975 mRNA cap methyltransferase
Length = 402
Score = 137 bits (345), Expect = 3e-32
Identities = 101/274 (36%), Positives = 140/274 (50%), Gaps = 27/274 (9%)
Query: 119 VRQHYNERTFLSKKHNRNYSPIIKLRNFNNAIKYILI-DKFTRAGDV------VLELACG 171
V HYN+ + + R S I +RN NN +K LI D R D VL+LACG
Sbjct: 28 VASHYNKVLQVGIE-GRKESRIFFMRNMNNWVKSQLINDAKQRVNDNGVNNPRVLDLACG 86
Query: 172 KGGDLRKYGAAGISQFIGIDISNASITEALKRYHSM----KNLEYQVILITGDCFGESLG 227
KGGDL+K+ AG + D+++ SI +A +RY M KN + V I DC E+L
Sbjct: 87 KGGDLKKWDIAGAKDVVMADVADVSIQQAEERYKQMFGYKKNNIFTVQFIVADCTKENLE 146
Query: 228 VAVESFPECRFPCDIVSCQFALHYAFETEEKARRMLLNVVKSLKIGGYFFGTIPDSEFIR 287
+E+ + P D+VSCQFALHY+F E AR L N V LK GG F GT+PD++ I
Sbjct: 147 DRIEN----KDPFDLVSCQFALHYSFVDEASARIFLKNAVGMLKPGGVFIGTLPDADRIV 202
Query: 288 YKMNKIPESVEKPSWGNSIYKVTFSNNEYQKNGNEFPSPFGQMYTFWLEDAIDNVPEYVI 347
+ M + E + N + K+T+ N E G FG + F L++ + N PE++
Sbjct: 203 WSM----RNGENGQFANEVCKITYENVEELAEGK--VPLFGAKFHFSLDEQV-NCPEFLA 255
Query: 348 PFESFRSLADEYGMELELQKGFNEFFVEEIPNWV 381
F + L +E MEL F F E I W+
Sbjct: 256 YFPLVKHLLEELDMELL----FVHNFAEAINKWL 285
>7298196 [A] KOG1975 mRNA cap methyltransferase
Length = 410
Score = 102 bits (255), Expect = 8e-22
Identities = 83/268 (30%), Positives = 129/268 (47%), Gaps = 27/268 (10%)
Query: 117 HVVRQHYNERTFLSKKHNRNYSPIIKLRNFNNAIKYILIDKFTRAGDVVLELACGKGGDL 176
HVV HYNE +K +R S I +RNFNN IK LI+++ ++ G L
Sbjct: 83 HVVAHHYNELKEAGRK-DRQKSKIFFMRNFNNWIKSQLINEYMSQ----IKQNKRMGDAL 137
Query: 177 RKYGAAGISQFIGIDISNASITEALKRYHSMKNLEYQVIL---ITGDCFG--ESLGVAVE 231
R A IS I DI+ S+ + +RY + + T + F +L E
Sbjct: 138 R----AAISHLICTDIAEVSVEQCQRRYQDILQRSEKSKFANKFTAEFFACDSTLVRLRE 193
Query: 232 SFPECRFPCDIVSCQFALHYAFETEEKARRMLLNVVKSLKIGGYFFGTIPDS-EFIRYKM 290
+ + ++VSCQFA HY FE+ +A M+ N + LK GG+F T+PD+ E IR
Sbjct: 194 RYKDPSLQLNLVSCQFAFHYCFESMAQADCMMRNAAECLKPGGFFIATMPDAYEIIRRLR 253
Query: 291 NKIPESVEKPSWGNSIYKVTFSNNEYQKNGNEFPSP-FGQMYTFWLEDAIDNVPEYVIPF 349
P++ +GN +Y + F + P P FG Y F LE +D PE+++ F
Sbjct: 254 AAGPDA---RRFGNDVYSIEF-------DCETDPLPLFGAKYQFHLEGVVD-CPEFLVHF 302
Query: 350 ESFRSLADEYGMELELQKGFNEFFVEEI 377
+ L +YG++L + F +++ E +
Sbjct: 303 PTLVKLGRKYGLQLLKRSTFADYYKENL 330
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.137 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,165,246
Number of Sequences: 60738
Number of extensions: 1151522
Number of successful extensions: 3072
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3043
Number of HSP's gapped (non-prelim): 7
length of query: 426
length of database: 30,389,216
effective HSP length: 109
effective length of query: 317
effective length of database: 23,768,774
effective search space: 7534701358
effective search space used: 7534701358
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)