ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI2950 good H KOG2525 Coenzyme transport and metabolism Folylpolyglutamate synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI2950 1041529 1040051 -493
(493 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR241w [H] KOG2525 Folylpolyglutamate synthase 663 0.0
SPBC1709.17 [H] KOG2525 Folylpolyglutamate synthase 367 e-101
HsM4826728 [H] KOG2525 Folylpolyglutamate synthase 310 2e-84
CE29283 [H] KOG2525 Folylpolyglutamate synthase 270 4e-72
CE01923 [H] KOG2525 Folylpolyglutamate synthase 270 4e-72
At3g55630 [H] KOG2525 Folylpolyglutamate synthase 224 3e-58
At5g05980 [H] KOG2525 Folylpolyglutamate synthase 223 4e-58
7292786 [H] KOG2525 Folylpolyglutamate synthase 214 3e-55
At3g10160 [H] KOG2525 Folylpolyglutamate synthase 183 6e-46
7295749_2 [H] KOG2525 Folylpolyglutamate synthase 155 1e-37
YMR113w [H] KOG2525 Folylpolyglutamate synthase 109 1e-23
SPAC227.09 [H] KOG2525 Folylpolyglutamate synthase 108 1e-23
At5g41480 [H] KOG2525 Folylpolyglutamate synthase 106 9e-23
>YOR241w [H] KOG2525 Folylpolyglutamate synthase
Length = 548
Score = 663 bits (1710), Expect = 0.0
Identities = 324/484 (66%), Positives = 390/484 (79%), Gaps = 2/484 (0%)
Query: 7 LKMANRNYQDAVQALNSLQSNYANIMAIRASGDRKNMMNIWEMQEWSRRIGYSVSDFNKL 66
L+M + Y+DAV ALNSLQSNYANIMAIR +GDRKN M + EM EWSRRIGYS SDFNKL
Sbjct: 61 LRMVGKTYRDAVTALNSLQSNYANIMAIRQTGDRKNTMTLLEMHEWSRRIGYSASDFNKL 120
Query: 67 NVIHITGTKGKGSTAAFTQGILNQYKDTLSKVGLYTSPHLRSVRERIRINGEPITEELFS 126
N++HITGTKGKGSTAAFT IL QYK+ L ++GLYTSPHL+SVRERIRINGEPI+EE F+
Sbjct: 121 NIVHITGTKGKGSTAAFTSSILGQYKEQLPRIGLYTSPHLKSVRERIRINGEPISEEKFA 180
Query: 127 KYFFEVWDRLDETSSNEDKFPHMTEGQKPGYFKYLTLLSFHVFMQEGCGTCIYEVGVGGE 186
KYFFEVWDRLD T+S+ DKFPHM G KPGYFK+LTLLSFH F+QE C +C+YEVGVGGE
Sbjct: 181 KYFFEVWDRLDSTTSSLDKFPHMIPGSKPGYFKFLTLLSFHTFIQEDCKSCVYEVGVGGE 240
Query: 187 FDSTNIIEKPTVCGVSALGIDHTFMLGNTIEEIAWNKGGIFKEGAPAFTVKSQPKTGSQV 246
DSTNIIEKP VCGV+ LGIDHTFMLG+TIEEIAWNKGGIFK GAPAFTV+ QP G +
Sbjct: 241 LDSTNIIEKPIVCGVTLLGIDHTFMLGDTIEEIAWNKGGIFKSGAPAFTVEKQPPQGLTI 300
Query: 247 LQERAAERHTTLSEVPIFEHLKTIKLGIAGDFQIKNASLATALAHQHLVSLDILKEPLEL 306
L+ERA ER TTL+EVP F+ L+ +KLGIAG+FQ NASLA LA + L + +IL+E ++
Sbjct: 301 LKERAEERKTTLTEVPPFKQLENVKLGIAGEFQKSNASLAVMLASEILHTSNILEEKIKC 360
Query: 307 ASSAKIPDLFRKGLEQTVWEGRCQIIRSNNITWYIDGAHTKESIEAASGWFRDVTATFNR 366
+S+A IP+ F GL+ T WEGRCQ++ WYIDGAHTK+S+ AAS WFRD+ R
Sbjct: 361 SSNASIPEKFIIGLQNTKWEGRCQVLEKGKNVWYIDGAHTKDSMVAASTWFRDMVRLSKR 420
Query: 367 KKVLLFNQQSRDAKALLGYLYETISPAVEFDAAIFTTNVTWKTGNYSADLVSLNTSKEQV 426
KK+LLFNQQSRDA AL+ LY ++SP + FD IFTTNVTWK+G+YSADLVS+NTS+E V
Sbjct: 421 KKILLFNQQSRDANALVNNLYSSVSPEITFDDVIFTTNVTWKSGSYSADLVSMNTSQEDV 480
Query: 427 DKLEVQQNLQKEWILLNETEHRNVEVTSSIEAALDLIKNM-GEPVDVFVTGSLHLVGGFL 485
+KL+VQ++L K W +++ + VT+SIE A +LI+ + EP D+FVTGSLHLVGG L
Sbjct: 481 EKLKVQESLVKNWNKIDDNRAK-THVTASIEEANELIETLYDEPADIFVTGSLHLVGGLL 539
Query: 486 VVLD 489
VV D
Sbjct: 540 VVFD 543
>SPBC1709.17 [H] KOG2525 Folylpolyglutamate synthase
Length = 505
Score = 367 bits (943), Expect = e-101
Identities = 214/503 (42%), Positives = 294/503 (57%), Gaps = 37/503 (7%)
Query: 5 LSLKMANRNYQDAVQALNSLQSNYANIMAIRASGDRKNMMNIWEMQEWSRRIGYSVSDFN 64
+ + + ++ A+ LNSLQSN + +R G N ++ EM+ W R IGY SD N
Sbjct: 18 IGMNPQTKTFEGAINRLNSLQSNAKVLEVLRKRGKIPNDQSMVEMRHWLRCIGYQPSDLN 77
Query: 65 KLNVIHITGTKGKGSTAAFTQGILNQYKDTLSK-----VGLYTSPHLRSVRERIRINGEP 119
+LNVIH+ GTKGKGST AFT IL Q + + + +G+YTSPHLRSV ERI++NG+P
Sbjct: 78 RLNVIHVAGTKGKGSTCAFTSSILQQIQKSGERSIPKCIGMYTSPHLRSVCERIQLNGKP 137
Query: 120 ITEELFSKYFFEVWDRLDETSSNEDKFPHMTEGQKPGYFKYLTLLSFHVFMQEGCGTCIY 179
I++ELF+KYFF+VW+RL+ ++ +KP YF++LTL+++HVF+ E I
Sbjct: 138 ISQELFTKYFFDVWERLENAVGSDS--------EKPMYFRFLTLMAWHVFISENVDAAII 189
Query: 180 EVGVGGEFDSTNIIEKPTVCGVSALGIDHTFMLGNTIEEIAWNKGGIFKEGAPAFTVKSQ 239
EVG+GGE+DSTN+IEKP V++LG+DHT +LGNTI EIAW K GI+KE A A T +
Sbjct: 190 EVGIGGEYDSTNLIEKPYATAVTSLGLDHTSLLGNTIAEIAWQKAGIYKESAIALTCEQA 249
Query: 240 PKTGSQVLQERAAERHTTLSEVPIFEHLKTIKLGIAGDFQIKNASLATALAHQ--HLVSL 297
P+ VL+ RAAER+T+L V L +G++G Q+ N SLA +L +
Sbjct: 250 PE-AMNVLKNRAAERNTSLKVVIPPAELTPDMIGLSGVHQLGNTSLAVSLVQEFYEKAGC 308
Query: 298 DILKEPLELASSAKIPDLFRKGLEQTVWEGRCQIIRSNNITWYIDGAHTKESIEAASGWF 357
++P + GL+ W GRCQI NNI W DGAHTKES+EA W
Sbjct: 309 PFDRDPYQ-------DPAILDGLKYVKWPGRCQIEEINNIKWCFDGAHTKESLEATGLWL 361
Query: 358 RDVTATFN--RKKVLLFNQQSRD--AKALLGYL--YETISPAVEFDAAIFTTNVTWKTGN 411
+ ++LLFNQQSRD L +L E+ + F IF+TNVTWK
Sbjct: 362 ASKKNLYEDADARILLFNQQSRDDPIALLRSFLKGLESSGTGISFTHVIFSTNVTWKDAG 421
Query: 412 YSADLVSLNTSKEQVDKLEVQQNLQKEWILLNETEHRNVEVT--SSIEAALDL---IKNM 466
Y+ +L+S+NT + L VQ++L K W E++ E T +I+ A++ IK
Sbjct: 422 YNPELLSINTITDNKPVLHVQEDLCKWW---KESKGTTSEATVAPTIQEAIETVMSIKQK 478
Query: 467 GEPVDVFVTGSLHLVGGFLVVLD 489
V VTGSLHL GG VVLD
Sbjct: 479 SRNTFVCVTGSLHLTGGVFVVLD 501
>HsM4826728 [H] KOG2525 Folylpolyglutamate synthase
Length = 545
Score = 310 bits (795), Expect = 2e-84
Identities = 214/556 (38%), Positives = 285/556 (50%), Gaps = 98/556 (17%)
Query: 14 YQDAVQALNSLQSNYANIMAI-RASGDRKNMMNIWEMQEWSRRIGYSVSDFNKLNVIHIT 72
YQDAV+ LN+LQ+N + + R GD + + E+ + R G V D ++LN+IH+T
Sbjct: 3 YQDAVRMLNTLQTNAGYLEQVKRQRGDPQTQLEAMEL--YLARSGLQVEDLDRLNIIHVT 60
Query: 73 GTKGKGSTAAFTQGILNQYKDTLSKVGLYTSPHLRSVRERIRINGEPITEELFSKYFFEV 132
GTKGKGST AFT+ IL Y K G ++SPHL VRERIRING+PI+ ELF+KYF+ +
Sbjct: 61 GTKGKGSTCAFTECILRSYG---LKTGFFSSPHLVQVRERIRINGQPISPELFTKYFWRL 117
Query: 133 WDRLDETSSNEDKFPHMTEGQKPGYFKYLTLLSFHVFMQEGCGTCIYEVGVGGEFDSTNI 192
+ RL+ET P YF++LTL++FHVF+QE + EVG+GG +D TNI
Sbjct: 118 YHRLEETKDGSCV-------SMPPYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAYDCTNI 170
Query: 193 IEKPTVCGVSALGIDHTFMLGNTIEEIAWNKGGIFKEGAPAFTVKSQPKTGSQVLQERAA 252
I KP VCGVS+LGIDHT +LG+T+E+IAW KGGIFK+G PAFTV QP+ VL++RA
Sbjct: 171 IRKPVVCGVSSLGIDHTSLLGDTVEKIAWQKGGIFKQGVPAFTV-LQPEGPLAVLRDRAQ 229
Query: 253 ERHTTLSEVPIFEHLK----TIKLGIAGDFQIKNASLATALAHQHLVSLDI--------- 299
+ L P+ E L+ + LG+ G+ Q NA+LA LAH L D
Sbjct: 230 QISCPLYLCPMLEALEEGGPPLTLGLEGEHQRSNAALALQLAHCWLQRQDRHGAGEPKAS 289
Query: 300 ---LKEPLELASSAKIPDLFRKGLEQTVWEGRCQIIRSNNITWYIDGAHTKESIEAASGW 356
L L LA + R GL T W GR Q++R +TWY+DGAHT S +A W
Sbjct: 290 RPGLLWQLPLAPVFQPTSHMRLGLRNTEWPGRTQVLRRGPLTWYLDGAHTASSAQACVRW 349
Query: 357 FRDVTATFNRK------KVLLFNQQ-SRDAKALLGYLYETISPAVEFDAAIFTTNVTWKT 409
FR R +VLLFN RD ALL L +FD A+F N+T +
Sbjct: 350 FRQALQGRERPSGGPEVRVLLFNATGDRDPAALLKLL-----QPCQFDYAVFCPNLTEVS 404
Query: 410 GNYSADLVSLNTSKEQV-----------DKLEVQQNLQKEW-------------ILLNET 445
+AD + + +QV + L+ +Q W +L
Sbjct: 405 STGNADQQNFTVTLDQVLLRCLEHQQHWNHLDEEQASPDLWSAPSPEPGGSASLLLAPHP 464
Query: 446 EH---RNVEVTSSIEAALDLIKNMGEP-----------------------------VDVF 473
H + V S I AL I +P + V
Sbjct: 465 PHTCSASSLVFSCISHALQWISQGRDPIFQPPSPPKGLLTHPVAHSGASILREAAAIHVL 524
Query: 474 VTGSLHLVGGFLVVLD 489
VTGSLHLVGG L +L+
Sbjct: 525 VTGSLHLVGGVLKLLE 540
>CE29283 [H] KOG2525 Folylpolyglutamate synthase
Length = 521
Score = 270 bits (690), Expect = 4e-72
Identities = 179/500 (35%), Positives = 267/500 (52%), Gaps = 36/500 (7%)
Query: 2 RLPLSLKMANRNYQDAVQALNSLQSNYANIMAIRASGDRKNMMNIWEMQEWSRRIGYSVS 61
+L +S + A Y+++V+ LN LQSN A I +R + +N+ + +++ + S
Sbjct: 35 QLRMSSEKAVPCYEESVRLLNGLQSNAATIKKLRVQRENLQAINLPQCRKYLESLNISAE 94
Query: 62 DFNKLNVIHITGTKGKGSTAAFTQGILNQYKDTLSKVGLYTSPHLRSVRERIRINGEPIT 121
D N LN+IH++GTKGKGS AF + IL + G Y+SPHL VRERI+++G+P++
Sbjct: 95 DLNALNIIHVSGTKGKGSACAFVESILRSQG---LRTGFYSSPHLVHVRERIQVDGQPVS 151
Query: 122 EELFSKYFFEVWDRLDETSSNEDKFPHMTEGQKPGYFKYLTLLSFHVFMQEGCGTCIYEV 181
E++F++ FF V+D + S+ P YFK+LTLL+F +F++ I EV
Sbjct: 152 EQMFAEEFFHVYDIIKREHSDN----------MPAYFKFLTLLAFRIFVKLNVQVMILEV 201
Query: 182 GVGGEFDSTNIIEKPTVCGVSALGIDHTFMLGNTIEEIAWNKGGIFKEGAPAFTVKSQPK 241
G+GGE+D TN++EKP VCGV+ L DH +LGN + EIAW+K GIFKE PAF + +
Sbjct: 202 GIGGEYDCTNVVEKPKVCGVTTLDYDHMSILGNKLSEIAWHKAGIFKESVPAFYSPTTTE 261
Query: 242 TGSQVLQERAAERHTTLSEVP---IFEHLKTIKLGIAGDFQIKNASLATAL--AHQHLVS 296
+VL RA +H L + P ++ + I GI G Q N S+A L A
Sbjct: 262 -AEEVLIARAISKHVPLFQTPPVSAYQFARDISPGIRGAHQFSNVSMALQLVRAWAEKCG 320
Query: 297 LDILKEPLELASSA-KIPDLFRKGLEQTVWEGRCQIIRSN-NITWYIDGAHTKESIEAAS 354
+ PL +S +P +E W GR QI+ ++ N+T+ +DGAHT +S+EA S
Sbjct: 321 FPLPGVPLSTDTSGFNVPLWMCDAIESCRWPGRSQIVSTDRNVTYLLDGAHTPKSMEACS 380
Query: 355 GWFRDVTATF---NRKKVLLFN-QQSRDAKALLGYLYET-ISPAVEFDAAIFTTNVTWKT 409
W + N KK+LLF R L+ YL IS V + ++
Sbjct: 381 EWAAEEIVNLKKENVKKILLFQCTADRCPSTLIKYLKPLGISQIVSCPTQLHSSI----- 435
Query: 410 GNYSADLVSLNTSK-EQVDKLEVQQNLQKEWILLNET-EHRNVEVTSSIEAALDLIKNM- 466
+ SAD +LN S+ EQ +K KE + E+ ++V I +A I++
Sbjct: 436 -DKSADSANLNASRDEQAEKANQCVQAWKESLDQPESVTEDQMKVFDCISSAYKFIESQA 494
Query: 467 -GEPVDVFVTGSLHLVGGFL 485
+ + V VTGSLHLVGG L
Sbjct: 495 ASQEILVLVTGSLHLVGGVL 514
>CE01923 [H] KOG2525 Folylpolyglutamate synthase
Length = 510
Score = 270 bits (690), Expect = 4e-72
Identities = 179/500 (35%), Positives = 267/500 (52%), Gaps = 36/500 (7%)
Query: 2 RLPLSLKMANRNYQDAVQALNSLQSNYANIMAIRASGDRKNMMNIWEMQEWSRRIGYSVS 61
+L +S + A Y+++V+ LN LQSN A I +R + +N+ + +++ + S
Sbjct: 24 QLRMSSEKAVPCYEESVRLLNGLQSNAATIKKLRVQRENLQAINLPQCRKYLESLNISAE 83
Query: 62 DFNKLNVIHITGTKGKGSTAAFTQGILNQYKDTLSKVGLYTSPHLRSVRERIRINGEPIT 121
D N LN+IH++GTKGKGS AF + IL + G Y+SPHL VRERI+++G+P++
Sbjct: 84 DLNALNIIHVSGTKGKGSACAFVESILRSQG---LRTGFYSSPHLVHVRERIQVDGQPVS 140
Query: 122 EELFSKYFFEVWDRLDETSSNEDKFPHMTEGQKPGYFKYLTLLSFHVFMQEGCGTCIYEV 181
E++F++ FF V+D + S+ P YFK+LTLL+F +F++ I EV
Sbjct: 141 EQMFAEEFFHVYDIIKREHSDN----------MPAYFKFLTLLAFRIFVKLNVQVMILEV 190
Query: 182 GVGGEFDSTNIIEKPTVCGVSALGIDHTFMLGNTIEEIAWNKGGIFKEGAPAFTVKSQPK 241
G+GGE+D TN++EKP VCGV+ L DH +LGN + EIAW+K GIFKE PAF + +
Sbjct: 191 GIGGEYDCTNVVEKPKVCGVTTLDYDHMSILGNKLSEIAWHKAGIFKESVPAFYSPTTTE 250
Query: 242 TGSQVLQERAAERHTTLSEVP---IFEHLKTIKLGIAGDFQIKNASLATAL--AHQHLVS 296
+VL RA +H L + P ++ + I GI G Q N S+A L A
Sbjct: 251 -AEEVLIARAISKHVPLFQTPPVSAYQFARDISPGIRGAHQFSNVSMALQLVRAWAEKCG 309
Query: 297 LDILKEPLELASSA-KIPDLFRKGLEQTVWEGRCQIIRSN-NITWYIDGAHTKESIEAAS 354
+ PL +S +P +E W GR QI+ ++ N+T+ +DGAHT +S+EA S
Sbjct: 310 FPLPGVPLSTDTSGFNVPLWMCDAIESCRWPGRSQIVSTDRNVTYLLDGAHTPKSMEACS 369
Query: 355 GWFRDVTATF---NRKKVLLFN-QQSRDAKALLGYLYET-ISPAVEFDAAIFTTNVTWKT 409
W + N KK+LLF R L+ YL IS V + ++
Sbjct: 370 EWAAEEIVNLKKENVKKILLFQCTADRCPSTLIKYLKPLGISQIVSCPTQLHSSI----- 424
Query: 410 GNYSADLVSLNTSK-EQVDKLEVQQNLQKEWILLNET-EHRNVEVTSSIEAALDLIKNM- 466
+ SAD +LN S+ EQ +K KE + E+ ++V I +A I++
Sbjct: 425 -DKSADSANLNASRDEQAEKANQCVQAWKESLDQPESVTEDQMKVFDCISSAYKFIESQA 483
Query: 467 -GEPVDVFVTGSLHLVGGFL 485
+ + V VTGSLHLVGG L
Sbjct: 484 ASQEILVLVTGSLHLVGGVL 503
>At3g55630 [H] KOG2525 Folylpolyglutamate synthase
Length = 498
Score = 224 bits (570), Expect = 3e-58
Identities = 159/448 (35%), Positives = 236/448 (52%), Gaps = 41/448 (9%)
Query: 14 YQDAVQALNSLQSNYANIMAIRASGDRKNMMNIWEMQEWSRRIGYSVSDFNKLNVIHITG 73
YQ+ + AL+SL + + + S + + + ++ E + +++ +IH+ G
Sbjct: 13 YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAV-------SQMKIIHVAG 65
Query: 74 TKGKGSTAAFTQGILNQYKDTLSKVGLYTSPHLRSVRERIRINGEPITEELFSKYFFEVW 133
TKGKGST F + IL Y + GL+TSPHL VRER R+NG I++E F YF+ +
Sbjct: 66 TKGKGSTCTFAESILRCYG---LRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCF 122
Query: 134 DRLDETSSNEDKFPHMTEGQKPGYFKYLTLLSFHVFMQEGCGTCIYEVGVGGEFDSTNII 193
+L E +SNE P YF +L LL+F +F E I EVG+GG FD+TN+
Sbjct: 123 HKLKEKTSNEVPMPT--------YFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNV- 173
Query: 194 EKPTVCGVSALGIDHTFMLGNTIEEIAWNKGGIFKEGAPAFTVKSQPKTGSQVLQERAAE 253
KP VCG+S+LG DH +LG T+ EIA K GIFK G PAFTV +QP +VL E+A++
Sbjct: 174 -KPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVPAFTV-AQPDEAMRVLNEKASK 231
Query: 254 RHTTLSEVPIFEHLKTIKLGIAGDFQIKNASLATALAHQHLVSLDILKEPLELASSAKIP 313
L V + + +LG+ G+ Q NA LA AL L + I ++ L + +P
Sbjct: 232 LEVNLQVVEPLD--SSQRLGLQGEHQYLNAGLAVALCSTFLKEIGI-EDKNGLDQTNGLP 288
Query: 314 DLFRKGLEQTVWEGRCQIIRSN----NITWYIDGAHTKESIEAASGWF-RDVTATFNR-- 366
+ F GL GR I+ + I +Y+DGAH+ ES+EA + WF + + R
Sbjct: 289 EKFISGLSNAYLMGRAMIVPDSELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQERNQ 348
Query: 367 ---KKVLLFNQQS-RDAKALLGYL-YETISPAVEFDAAIFTTNVTWKTGNYSADLVSLNT 421
+++LLFN S RD LL L + I V+F A+F NV+ N ++ T
Sbjct: 349 KRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVS--VYNQVGSSTNVGT 406
Query: 422 SKEQVDKLEVQQNLQKEWILLNETEHRN 449
+V+ + Q LQ+ W L E ++
Sbjct: 407 ---RVESMSWQFGLQRIWESLARGEAKS 431
>At5g05980 [H] KOG2525 Folylpolyglutamate synthase
Length = 566
Score = 223 bits (569), Expect = 4e-58
Identities = 177/488 (36%), Positives = 257/488 (52%), Gaps = 66/488 (13%)
Query: 13 NYQDAVQALNSLQSNYANIMAIRASGDRKNMMNIWEMQEWSRRIGYSVSDFNKLNVIHIT 72
+Y++A+ AL+SL + R+ D+ N + +E+ ++ D K+NVIH+
Sbjct: 40 SYEEALAALSSLITK-------RSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVA 92
Query: 73 GTKGKGSTAAFTQGILNQYKDTLSKVGLYTSPHLRSVRERIRINGEPITEELFSKYFFEV 132
GTKGKGST FT+ I+ Y + GL+TSPHL VRER R++G I+EE F YF+
Sbjct: 93 GTKGKGSTCTFTESIIRNYG---FRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWC 149
Query: 133 WDRLDETSSNEDKFPHMTEGQKPGYFKYL-------TLLSFHVFMQEGCGTCIYEVGVGG 185
++RL KF ++ +K Y+ Y L + VF + I EVG+GG
Sbjct: 150 YNRL--------KFWYL---EKLYYWNYFHRGGGDAVELMYSVFPVDAA---ILEVGLGG 195
Query: 186 EFDSTNIIEKPTVCGVSALGIDHTFMLGNTIEEIAWNKGGIFKEGAPAFTVKSQPKTGSQ 245
+FD+TN ++KP VCG+S+LG DH +LG+T+ +IA K GIFK G PAFTV QP +
Sbjct: 196 KFDATNAVQKPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVPAFTV-PQPDEAMR 254
Query: 246 VLQERAAERHTTLSEV-PIFEHLKT-IKLGIAGDFQIKNASLATALAHQHLVSLDILKEP 303
VL+E+A+E L V P+ L + KLG+ G+ Q NA LA +LA L + L+ P
Sbjct: 255 VLEEKASETEVNLEVVQPLTARLLSGQKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVP 314
Query: 304 LELASSAKIPDLFRKGLEQTVWEGRCQII--------RSNNITWYIDGAHTKESIEAASG 355
S +P+ F KGL +GR Q++ S ++ +Y+DGAH+ ES+EA +
Sbjct: 315 SRTQMSI-LPEKFIKGLATASLQGRAQVVPDQYTESRTSGDLVFYLDGAHSPESMEACAK 373
Query: 356 WFRDVTATFNRK-----KVLLFNQQS-RDAKALLGYLYETISP-AVEFDAAIFTTN--VT 406
WF N+ +LLFN S RD LL +L + V F A+F N V
Sbjct: 374 WFSVAVKGDNQSGSSGHLILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVY 433
Query: 407 WKTGNYSADLVSLNTSKEQVDKLEVQQNLQKEWILLNETEH---------RNVEVTSSIE 457
K G +ADL + QVD L Q LQK W L ++E N EV +S+
Sbjct: 434 HKVGT-AADLPE---NDPQVD-LSWQFTLQKVWESLVQSERDGEKDGESDGNSEVFTSLP 488
Query: 458 AALDLIKN 465
A+ +++
Sbjct: 489 MAIKCLRD 496
>7292786 [H] KOG2525 Folylpolyglutamate synthase
Length = 522
Score = 214 bits (544), Expect = 3e-55
Identities = 154/498 (30%), Positives = 243/498 (47%), Gaps = 62/498 (12%)
Query: 8 KMANRNYQDAVQALNSLQSNYANIMAIRASGDRKNMMNIWEMQEWSRRIGYSVSDFNKLN 67
K N ++ A++ LNSLQSN A AIR S + + ++ R G + +L+
Sbjct: 60 KDTNAAFELAIKQLNSLQSNDA---AIRNSMSNSRVDTKADTIKYLERSGLPLETVEQLS 116
Query: 68 VIHITGTKGKGSTAAFTQGILNQYKDTLSKVGLYTSPHLRSVRERIRINGEPITEELFSK 127
IH+ GTKGKGST A T+ +L + G ++SPH+ ERIRI+G+ ++++ F++
Sbjct: 117 FIHVAGTKGKGSTCALTESLLRHQG---FRTGFFSSPHILFTNERIRIDGQLLSKDKFTE 173
Query: 128 YFFEVWDRLDETSSNEDKFPHMTEGQKPGYFKYLTLLSFHVFMQEGCGTCIYEVGVGGEF 187
F+ K+LT+L FHVF+ E + EVG+GGE
Sbjct: 174 QFW----------------------------KFLTILGFHVFVAENVDVVVLEVGIGGEH 205
Query: 188 DSTNIIEKPTVCGVSALGIDHTFMLGNTIEEIAWNKGGIFKEGAPAFTVKSQPKTGSQVL 247
D TNI+ G+++LG++HT +LG T+ EIAW K GI K G+ FT +QP+ +V+
Sbjct: 206 DCTNIVRNVRTVGITSLGLEHTELLGRTLPEIAWQKAGIIKTGSHVFTHVTQPEC-LEVI 264
Query: 248 QERAAERHTTLSEVPIFEHLKTIKLGIAGDFQI------KNASLATALAHQHLVSLDILK 301
++R E TL EVP E K A +Q N SLA LA L
Sbjct: 265 RQRTKEHSATLYEVPPTEDYFRSK-AYAPIWQTFSNLIRLNGSLAIQLAQDWLSQSG--- 320
Query: 302 EPLELASSAKIPDLFRKGLEQTVWEGRCQIIRSNNITWYIDGAHTKESIEAASGWFRDVT 361
+ + K+ GL T W GRCQ+I + + ++DGAHT ES+E + WF
Sbjct: 321 KQQHTPNEVKMDPQLLDGLISTHWPGRCQLIEWHGMRLHLDGAHTLESMEVCTDWFEKNV 380
Query: 362 ATFNRKKVLLFNQQSRDAKALLGYLYETISPAVEFDAAIFTTNVTWKTGNYSADLVSLNT 421
K+L+FN R ++ L + ++ +FD F N+ T N + ++ +
Sbjct: 381 RDSVNPKILIFN---RTGESGFAPLLKLLNRTCDFDMVCFVPNLATSTPNAPSQVMVRFS 437
Query: 422 SKEQVDKLEVQQNLQKEWILLNETEHRN--VEVTSSIEAALDLIK--------NMGEPVD 471
+ Q+++ + + W L TE + +V +++ A I+ N G+ ++
Sbjct: 438 PEMQLNRARI---IASAWSDLCATEQKKDVGQVYNTLTDAFTAIRQRFPQATDNEGQ-LE 493
Query: 472 VFVTGSLHLVGGFLVVLD 489
V VTGS+HL+G + LD
Sbjct: 494 VLVTGSIHLLGAAISALD 511
>At3g10160 [H] KOG2525 Folylpolyglutamate synthase
Length = 442
Score = 183 bits (464), Expect = 6e-46
Identities = 128/362 (35%), Positives = 187/362 (51%), Gaps = 34/362 (9%)
Query: 100 LYTSPHLRSVRERIRINGEPITEELFSKYFFEVWDRLDETSSNEDKFPHMTEGQKPGYFK 159
++TSPHL VRER RI+G I+EE F +YF+E W L E + + P + F+
Sbjct: 1 MFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKEKAVDGLTMPPL--------FQ 52
Query: 160 YLTLLSFHVFMQEGCGTCIYEVGVGGEFDSTNIIEKPTVCGVSALGIDHTFMLGNTIEEI 219
+LT+L+F +F+ E + EVG+GG+ DSTN+I+KP VCG+++LG+DH +LGNT+ +I
Sbjct: 53 FLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGMDHMDILGNTLADI 112
Query: 220 AWNKGGIFKEGAPAFTVKSQPKTGSQVLQERAAERHTTLSEVPIFEHLKTIKLGIAGDFQ 279
A++K GIFK PAFTV Q VLQ+ A T+ + L + LG++GD Q
Sbjct: 113 AFHKAGIFKPQIPAFTV-PQLSEAMDVLQKTANNLEVTIVAPLEPKKLDGVTLGLSGDHQ 171
Query: 280 IKNASLATALAHQHLVSLDILKE--PLELASSAKIPDLFRKGLEQTVWEGRCQIIRS--- 334
+ NA LA +L+ L K+ P E + +IP F +GL GR Q++
Sbjct: 172 LVNAGLAVSLSRCWLQRTGNWKKIFPNE-SKETEIPVAFCRGLATARLHGRAQVVHDVVS 230
Query: 335 -------------NNITWYIDGAHTKESIEAASGWFRDVTATFN--RKKVLLFN-QQSRD 378
++ +Y+DGAH+ ES+EA WF +LLFN + RD
Sbjct: 231 DPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAVRGDKSLSTAILLFNCMEVRD 290
Query: 379 AKALLGYLYET-ISPAVEFDAAIFTTNVTWKTGNYSADLVSLNTSKEQVDKLEVQQNLQK 437
+ LL L T S F A+F +++ T N S S + L Q LQ+
Sbjct: 291 PQVLLPKLVTTCASSGTHFSRALFVPSMS--TYNKVISGASAIPSDTRRKDLTWQFRLQR 348
Query: 438 EW 439
W
Sbjct: 349 LW 350
>7295749_2 [H] KOG2525 Folylpolyglutamate synthase
Length = 598
Score = 155 bits (392), Expect = 1e-37
Identities = 144/496 (29%), Positives = 214/496 (43%), Gaps = 64/496 (12%)
Query: 17 AVQALNSLQSNYANIMAIRASGDRKNMMNIWEMQ-EWSRRIGYSVSDFNKLNVIHITGTK 75
A++ LNS Q RK + E E + G + D + VI + G+K
Sbjct: 1 AIKQLNSYQVLEPTSGRATTKSSRKEQDPVIEQTLECLEKSGLTKKDLKAIPVIQVAGSK 60
Query: 76 GKGSTAAFTQGILNQYKDTLSKVGLYTSPHLRSVRERIRINGEPITEELFSKYFFEVWDR 135
G+GST A + IL + K G+ +SPHL ERIRI+GEP+++ F++ F+++
Sbjct: 61 GRGSTCAIVESILRCHG---VKTGVLSSPHLFLTSERIRIDGEPLSDVQFTELFWKINTD 117
Query: 136 LDETSSNEDKFPHMTEGQKPGYFKYLTLLSFHVFMQEGCGTCIYEVGVGGEFDSTNIIEK 195
L P Y K +T+++FH F Q G I EVG G D+TNI
Sbjct: 118 LANMQPT------------PSYNKIMTVMAFHAFHQAGVEVAILEVGNAGASDATNIASH 165
Query: 196 PTVCGVSALGIDHTFMLGNTIEEIAWNKGGIFKEGAPAFTVKSQPKTGSQVLQERAAERH 255
G++ LG + + LGN++ +IAW K I K A +T +Q + +VL ++A +
Sbjct: 166 AQTIGITTLGWEQSSNLGNSLRDIAWAKASIMKPEANIYTNVTQTEC-CEVLAQKAKQIG 224
Query: 256 TTLSEVPIFEHLKTIKLGIAGDFQIK------------NASLATALAHQHLVSLDILKE- 302
L VP F + GD K N SLA LA+ D LK
Sbjct: 225 AQLRRVPTFNDY------VEGDMNNKLLMNKANYSMRLNGSLAVQLAY------DFLKRH 272
Query: 303 ------PLELASSAKIPDLFRKGLEQTVWEGRCQIIRSNNITWYIDGAHTKESIEAASGW 356
LE S+ P R G+E G +R + Y+D A T ES+ A W
Sbjct: 273 KPEYVVGLEHNSTLLTPGASR-GIEIFEQPGHFDFMRHDMFNVYLDSADTFESMMACRDW 331
Query: 357 FRDVTATFNRKKVLLFNQQSR-DAKALLGYLYETISPAVEFDAAIFTTNVTWKTGNYSAD 415
F T + K+LLFN+ + +AK LL I + F+ A F N + G A+
Sbjct: 332 FYTRTRANRQPKILLFNKVNEFNAKDLL----TIIRSNLRFEEACFVPNPNYFEGEILAE 387
Query: 416 LVSLNTSKEQVDKLEVQQNLQKEWILLNETEHR--NVEVTSSIEAALDLIKN-------- 465
+++L+ + W L E + N +++ SI A + + N
Sbjct: 388 EDGKAMVWHGMEELQRAKRNAGNWRALCEENGKRDNSQLSISINAFFEYLTNKYGKQKYG 447
Query: 466 MGEPVDVFVTGSLHLV 481
M +DV VTGS LV
Sbjct: 448 MKNELDVLVTGSRQLV 463
>YMR113w [H] KOG2525 Folylpolyglutamate synthase
Length = 427
Score = 109 bits (272), Expect = 1e-23
Identities = 78/241 (32%), Positives = 112/241 (46%), Gaps = 31/241 (12%)
Query: 64 NKLNVIHITGTKGKGSTAAFTQGILNQYKDTLSKVGLYTSPHLRSVRERIRINGEPITEE 123
N L V+HI GT GKGS + +L Q ++G +T+PHL V + I IN +PI E
Sbjct: 22 NSLRVLHIAGTNGKGSVCTYLSSVLQQKS---YQIGKFTTPHLVHVTDSITINNKPIPLE 78
Query: 124 LFSKYFFEVWDRLDETSSNEDKFPHMTEGQKPGYFKYLTLLSFHVFMQEGCGTCIYEVGV 183
+ RL + N+ TE F+ LT +F F C C+ EVG+
Sbjct: 79 RYQNI------RLQLEALNKSHSLKCTE------FELLTCTAFKYFYDVQCQWCVIEVGL 126
Query: 184 GGEFDSTNIIE--KPTVCGVSALGIDHTFMLGNTIEEIAWNKGGIFKEGAPAFTVKSQPK 241
GG D+TN+I CG++ + +DH LGNT+ EI+ K GI EG P FTV
Sbjct: 127 GGRLDATNVIPGANKACCGITKISLDHESFLGNTLSEISKEKAGIITEGVP-FTVIDGTN 185
Query: 242 TGS--QVLQERAAERHTTLS-----------EVPIFEHLKTIKLGIAGDFQIKNASLATA 288
S V++ER + LS + + KL + G++QI N +A
Sbjct: 186 EASVINVVKERCKALGSELSVTDSQLNGNMIDTNSWGCFDLAKLPLNGEYQIFNLRVAMG 245
Query: 289 L 289
+
Sbjct: 246 M 246
>SPAC227.09 [H] KOG2525 Folylpolyglutamate synthase
Length = 417
Score = 108 bits (271), Expect = 1e-23
Identities = 90/301 (29%), Positives = 136/301 (44%), Gaps = 51/301 (16%)
Query: 69 IHITGTKGKGSTAAFTQGILNQYKDTLSKVGLYTSPHLRSVRERIRINGEPITEELFS-- 126
+ I GT GKGS ++ L Q K G YTSPH R+ I ING+ +EE+F+
Sbjct: 27 VQIAGTNGKGSICSYIYTSLLQ---AAIKTGRYTSPHFLEPRDTISINGQIASEEIFNTC 83
Query: 127 -KYFFEVWDRLDETSSNEDKFPHMTEGQKPGYFKYLTLLSFHVFMQEGCGTCIYEVGVGG 185
K EV DR T + E F+ LT +F F G + E G+GG
Sbjct: 84 WKQVIEV-DRRFRTKATE--------------FELLTATAFQCFHHSGVRVAVIETGMGG 128
Query: 186 EFDSTNIIEKPTVCGVSALGIDHTFMLGNTIEEIAWNKGGIFKEGAPA-------FTVKS 238
D+TN+ E+P + +S + +DH LGNT+E IA K GIFK+ P V +
Sbjct: 129 RLDATNVFEEPVLSIISRICLDHQAFLGNTLEAIAKEKAGIFKKNVPCVVDGLNEVNVLN 188
Query: 239 QPKTGSQVLQ---------ERAAERHTTLSEVPIFEHLKTIKLGIAGDFQIKNASLATAL 289
Q K ++ + + ++ + P + T + GD+Q +N + A
Sbjct: 189 QLKLSAEETRAHPFYLAKGKSGENKNEWIINTPNW-GTNTFSTPLKGDYQGQNLACA--- 244
Query: 290 AHQHLVSLDILKEPLELASSAKIPDLFRKGLEQTVWEGRCQIIRSNNI-TWYIDGAHTKE 348
+ +LDIL + S +P + + G++ T W GR I ++ DGAH KE
Sbjct: 245 ----VTALDILSS----SFSIMLPHV-QNGVKNTSWPGRLDIRSVPSLGDILFDGAHNKE 295
Query: 349 S 349
+
Sbjct: 296 A 296
>At5g41480 [H] KOG2525 Folylpolyglutamate synthase
Length = 480
Score = 106 bits (264), Expect = 9e-23
Identities = 123/452 (27%), Positives = 192/452 (42%), Gaps = 75/452 (16%)
Query: 65 KLNVIHITGTKGKGSTAAFTQGILNQYKDTLSKVGLYTSPHLRSVRERIRINGEPITEEL 124
K V+H+ GTKGKGST+AF IL + VG Y+SPH+ S++ERI NGEP++
Sbjct: 74 KYKVVHVAGTKGKGSTSAFLSNILRAGGYS---VGCYSSPHILSIKERISCNGEPVSAST 130
Query: 125 FSKYFFEVWDRLDETSSNEDKFPHMTEGQKPGYFKYLTLLSFHVFMQEGCGTCIYEVGVG 184
+ F+ V L+++ E+ +F+ LT ++F +F +E + E G+G
Sbjct: 131 LNDLFYSVKPILEQSIQEENG--------SLSHFEILTGIAFSLFEKENVDIAVIEAGLG 182
Query: 185 GEFDSTNIIEKPTVCGVSALGIDHTFMLGNTIEEIAWNKGGIFKE--------GAPAFTV 236
G D+TN+IE + I F+ IE I +K G+ +
Sbjct: 183 GARDATNVIESSNLAASVITTIGGPFL--PHIEGILRSKAASVSSSVILASNIGSSSSIK 240
Query: 237 KSQPKTGSQVLQERAAERHTTLSEVPIFEHLKTIKLGIAGDFQIKNASLATALAHQHLVS 296
K G + Q + PI E L + L + G Q++NA AT VS
Sbjct: 241 GIINKNGIGLCQSCDIVIQNEKDDQPIVE-LSDVNLRMLGHHQLQNAVTATC------VS 293
Query: 297 LDILKEPLELASSAKIPDLFRKGLEQTVWEGRCQIIRSNNI--------TWYIDGAHTKE 348
L + + + I R GLE T GR Q + T +DGAHTKE
Sbjct: 294 LCLRDQGCGRVTDEAI----RIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDGAHTKE 349
Query: 349 SIEAASGWFRDVTATFNRKKVLLFNQQSRDAKALLGYLYETISPAVEFDAAIFTTNVTWK 408
S A + F K+++ + D K + + E +S ++ +A I T
Sbjct: 350 SARALKEMIKK---DFPEKRLVFVVAMASD-KDHVSFAKELLS-GLKPEAVILT------ 398
Query: 409 TGNYSADLVS--LNTSKEQVDKLEVQQNLQKEWI-LLNETEHRNVE------VTSSIEAA 459
AD+ + +++ V L++ WI +E R++E V S++ A
Sbjct: 399 ----EADIGGGKIRSTESSV--------LKESWIKAADELGSRSMEASENKTVLGSLKLA 446
Query: 460 LDLIKNMGEPVD---VFVTGSLHLVGGFLVVL 488
++ + D V VTGSLH+V L L
Sbjct: 447 YKILSDDTTSSDSGMVIVTGSLHIVSSVLASL 478
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.134 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,633,485
Number of Sequences: 60738
Number of extensions: 1208072
Number of successful extensions: 3182
Number of sequences better than 1.0e-05: 13
Number of HSP's better than 0.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 3116
Number of HSP's gapped (non-prelim): 16
length of query: 493
length of database: 30,389,216
effective HSP length: 110
effective length of query: 383
effective length of database: 23,708,036
effective search space: 9080177788
effective search space used: 9080177788
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)