ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI2963 good O KOG2976 Posttranslational modification, protein turnover, chaperones Protein involved in autophagy and nutrient starvation
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI2963 1046847 1047659 271
(271 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL149w [O] KOG2976 Protein involved in autophagy and nutrient s... 167 1e-41
SPBC4B4.10c [O] KOG2976 Protein involved in autophagy and nutrie... 92 8e-19
At5g17290 [O] KOG2976 Protein involved in autophagy and nutrient... 72 8e-13
Hs4757798 [O] KOG2976 Protein involved in autophagy and nutrient... 60 3e-09
7290808 [O] KOG2976 Protein involved in autophagy and nutrient s... 58 1e-08
>YPL149w [O] KOG2976 Protein involved in autophagy and nutrient starvation
Length = 294
Score = 167 bits (423), Expect = 1e-41
Identities = 98/282 (34%), Positives = 153/282 (53%), Gaps = 13/282 (4%)
Query: 1 MEELRERVWHGSLNVEIMLSDSIVVPNTPLSEKCYHIVVLRESFLALYLPAIVRKLGNNV 60
M ++++ +W+G LNV + + S ++ +P I V RE++L Y+P I K+ + +
Sbjct: 1 MNDIKQLLWNGELNVLVSIDPSFLMKGSPREIAVLRIRVPRETYLVNYMPLIWNKIKSFL 60
Query: 61 IVT-YENPYKQWWFEYDGVPVPWEYPCGVLFDFLCNSSTTSTGKEDDQR---LQMWKLKL 116
+ K +WFE++ P+PW YP GVLFD L S T T ++Q L ++ L
Sbjct: 61 SFDPLTDSEKYFWFEHNKTPIPWNYPVGVLFDCLAGKSATFTTSFENQVKDVLTFLRIHL 120
Query: 117 CHGNKYPPGILPLVDGLRQVKDHWKHQWKQACFILNGSAKRIMSLSIPDFEAFWQSLISR 176
G+ PP I+P+ Q + W HQWKQ CFILNGS+K IMSLS+ + FW S+I+R
Sbjct: 121 VMGDSLPPTIIPIASSKTQAEKFWFHQWKQVCFILNGSSKAIMSLSVNEARKFWGSVITR 180
Query: 177 HQPDYIKVREKLLTPNKTKHIPIRVWTADAS----FLQPSIPANSDTMTLFDVMTS-MDI 231
+ D+I++ K ++ ++ +HIP+ + T+ S QP+I TL D+ +D+
Sbjct: 181 NFQDFIEISNK-ISSSRPRHIPLIIQTSRTSGTFRISQPTISMTGVNPTLKDIEGDILDV 239
Query: 232 KLQENNR---AIIQGIVICSDEDIINLYDLFASIDGFLYVVI 270
K N I QGI I + +LY S DGFLY+ +
Sbjct: 240 KEGINGNDVMVICQGIEIPWHMLLYDLYSKLRSFDGFLYITL 281
>SPBC4B4.10c [O] KOG2976 Protein involved in autophagy and nutrient starvation
Length = 261
Score = 92.0 bits (227), Expect = 8e-19
Identities = 70/271 (25%), Positives = 135/271 (48%), Gaps = 30/271 (11%)
Query: 6 ERVWHGSLNVEIMLSDSIVVPNTPLSEKCYHIVVLRESFLALYLPAIVRKLGNNVIVTYE 65
E +W+G+++V I + + Y V R+S+ A LP + R L ++
Sbjct: 12 ELLWNGTISVRIDYEGNSLA---------YLANVPRQSYFAQILPNVQRLLAPSI----- 57
Query: 66 NPYKQWWFEYDGVPVPWEYPCGVLFDFLCNSSTTSTGKEDDQRLQMWKLKLCHGNKYPPG 125
P + W +Y+GVP+ W +P G+LFD L T + + +W+++L G +P
Sbjct: 58 -PLSECWLDYNGVPLKWHWPVGLLFDLL-----TVFDPDTPRAPVLWRIQLRSG-LFPTT 110
Query: 126 ILPLVDGLRQVKDHWKHQWKQACFILNGSAKRIMSLSIPDFEAFWQSLISRHQPDYIKVR 185
+ ++ + + ++ + K++ ++ NGS+ I++LS + + +W ++++ D+ +
Sbjct: 111 KILQMETMDTFRTYFFNCLKESDYVRNGSSSGIIALSKAETDTYWNAILNHDYYDFRPIA 170
Query: 186 EKLLTPNKTKHIPIRVWT-ADASFLQPSIPANSD-----TMTLFDVMTSMDIKLQENNRA 239
K+L +K+K IP++++ A+A +Q S P S L D+ S D L +
Sbjct: 171 IKILF-SKSKFIPLKIYLGANAPIIQTSAPLGSSLGEFLNKRLPDLFPSCDKFLIV--KP 227
Query: 240 IIQGIVICSDEDIINLYDLFASIDGFLYVVI 270
+I GI I + L F IDGFL++V+
Sbjct: 228 VIHGITIFLQSVLDELNRDFCYIDGFLHIVL 258
>At5g17290 [O] KOG2976 Protein involved in autophagy and nutrient starvation
Length = 337
Score = 72.0 bits (175), Expect = 8e-13
Identities = 52/187 (27%), Positives = 89/187 (46%), Gaps = 18/187 (9%)
Query: 2 EELRERVWHGSLNVEIMLSDSIVVPNTPLSEKCYHIVVLRESFLALYLPAIVRKLGNNVI 61
+E + VW G++ ++I L S V + ++ R +L L +P +++ + +
Sbjct: 3 KEAVKYVWEGAIPLQIHLHKSDVASHPAPPPAL--VLAPRIGYLPLLIP-LIKPYFKDSL 59
Query: 62 VTYENPYKQWWFEYDGVPVPWEYPCGVLFDFLCNSSTTSTGKEDDQRLQMWKLKLCHGNK 121
E+ WF+Y G P+ W P GVLFD LC + W L + H
Sbjct: 60 PPGEDSI---WFDYKGFPLKWYIPTGVLFDLLCAEPE-----------RPWNLTI-HFRG 104
Query: 122 YPPGILPLVDGLRQVKDHWKHQWKQACFILNGSAKRIMSLSIPDFEAFWQSLISRHQPDY 181
YP IL +G VK ++ + K+A +I+NG+ K +M++S D E W S+++ Y
Sbjct: 105 YPCNILIPCEGEDSVKWNFVNSLKEAQYIINGNCKNVMNMSQSDQEDLWTSVMNGDLDAY 164
Query: 182 IKVREKL 188
++ KL
Sbjct: 165 TRLSPKL 171
>Hs4757798 [O] KOG2976 Protein involved in autophagy and nutrient starvation
Length = 275
Score = 60.1 bits (144), Expect = 3e-09
Identities = 61/278 (21%), Positives = 113/278 (39%), Gaps = 34/278 (12%)
Query: 8 VWHGSLNVEIMLSDSIVVPNTPLSEKCYHIVVLRESFLALYLPAIVRKLGNNVIVTYENP 67
VW G + L + T + Y++++ R S+L L + + V +
Sbjct: 11 VWFGRIPTCFTLYQDEI---TEREAEPYYLLLPRVSYLTLVTDKVKKHFQK---VMRQED 64
Query: 68 YKQWWFEYDGVPVPWEYPCGVLFDFLCNSSTTSTGKEDDQRLQMWKLKLCHGNKYPPGIL 127
+ WFEY+G P+ W YP G+LFD L +SS W + + H +P L
Sbjct: 65 ISEIWFEYEGTPLKWHYPIGLLFDLLASSSALP-----------WNITV-HFKSFPEKDL 112
Query: 128 PLVDGLRQVKDHWKHQWKQACFILNGSAKRIMSLSIPDFEAFWQSLISRHQPDYIKVREK 187
++ H+ K+A L ++ I + D + W L + + + K
Sbjct: 113 LHCPSKDAIEAHFMSCMKEA-DALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRK 171
Query: 188 LL----TPNKTKHIPIRVW--TADASFLQP---SIPANSDTMTLFDVMTSMDIKL----- 233
L+ N ++IP R++ T + F+Q + A+ TL D++ +
Sbjct: 172 LMEYPAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPED 231
Query: 234 -QENNRAIIQGIVICSDEDIINLYDLFASIDGFLYVVI 270
++ N+ +I GI + + L + + D FL++ I
Sbjct: 232 GEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISI 269
>7290808 [O] KOG2976 Protein involved in autophagy and nutrient starvation
Length = 306
Score = 58.2 bits (139), Expect = 1e-08
Identities = 48/207 (23%), Positives = 92/207 (44%), Gaps = 21/207 (10%)
Query: 3 ELRERVWHGSLNVEIMLSDSIVVPNTPLSEKCYHIVVLRESFLALYLPAIVRKLGNNVIV 62
E+ +W G + + +V P + +++++ R S+L L VRK + I
Sbjct: 6 EVLRMIWEGQIGICFQADRDEIVGIKP---EPFYLMISRLSYLPLVTDK-VRKYFSRYI- 60
Query: 63 TYENPYKQWWFEYDGVPVPWEYPCGVLFDFLCNSSTTSTGKEDDQRLQMWKLKLCHGNKY 122
+ E+ WF+++G P+ YP GVL+D L ++ W L + H +K+
Sbjct: 61 SAEHQDGAVWFDFNGTPLRLHYPIGVLYDLL----------HPEEDSTPWCLTI-HFSKF 109
Query: 123 PPGILPLVDGLRQVKDHWKHQWKQACFILNGSAKRIMSLSIPDFEAFWQSLISRHQPDYI 182
P +L ++ ++ H+ K+A +L I ++ D W L++ +
Sbjct: 110 PEDMLVKLNSKELLESHYMSCLKEA-DVLKHRGLVISAMQKKDHNQLWLGLVNEKFDQFW 168
Query: 183 KVREKLLTP----NKTKHIPIRVWTAD 205
V +L+ P K+IP+R++T D
Sbjct: 169 AVNRRLMEPYGDLESFKNIPLRIYTDD 195
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.323 0.139 0.444
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,880,701
Number of Sequences: 60738
Number of extensions: 787452
Number of successful extensions: 1565
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1558
Number of HSP's gapped (non-prelim): 5
length of query: 271
length of database: 30,389,216
effective HSP length: 105
effective length of query: 166
effective length of database: 24,011,726
effective search space: 3985946516
effective search space used: 3985946516
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)