ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI3126 good R KOG2776 General function prediction only Metallopeptidase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI3126 1100421 1098709 -571
(571 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR101c [R] KOG2776 Metallopeptidase 525 e-149
SPBC23E6.05 [R] KOG2776 Metallopeptidase 102 2e-21
SPAC23H4.09 [R] KOG2776 Metallopeptidase 66 2e-10
Hs5453842 [R] KOG2776 Metallopeptidase 59 2e-08
Hs20553033 [R] KOG2776 Metallopeptidase 59 2e-08
CE21275 [R] KOG2776 Metallopeptidase 54 5e-07
>YDR101c [R] KOG2776 Metallopeptidase
Length = 593
Score = 525 bits (1353), Expect = e-149
Identities = 288/591 (48%), Positives = 385/591 (64%), Gaps = 34/591 (5%)
Query: 1 MALQISKEDTQVLLKDKNVLIEPTIEKYRTAGQIAQTALNFLISLINKMYHEKSME-PLP 59
MAL IS EDTQ+LLKDKN+L E + KYRTAGQIAQTAL ++ SLIN YH K+ + L
Sbjct: 1 MALAISHEDTQILLKDKNILQESVLNKYRTAGQIAQTALKYVTSLINDSYHSKTTQRQLT 60
Query: 60 IHQLCLLTDSFIADCLSTKFNNKVNEKGISMPTCIDIDEINENWCPEVDDIENLSRWNQK 119
+ +LCLLTDSFI L + NKVNE+GI++PT IDID+I+ WCPE+DD +NL WN+
Sbjct: 61 VPELCLLTDSFILTRLEQYYKNKVNERGIAIPTTIDIDQISGGWCPEIDDTQNLLNWNKG 120
Query: 120 SRSTKSIELCNGVTSSLSGFLKVGDVAKITLGCHIDGYTCNVSHTVVIYPTVQDAQSGKL 179
ST SS++G L+ GD+ KITLG HIDGYT VSHT+VIYP D L
Sbjct: 121 KDST--------FASSVTGTLRPGDLVKITLGVHIDGYTSEVSHTMVIYPV--DETKPIL 170
Query: 180 LPEXXXXXXXXXXXXXTHIAVESVTNLLAAANDLTKLPADFNATK--VTGSLIRTVVDTI 237
P HIA+E+V LLA A KLPA T +TG LIRT+VDTI
Sbjct: 171 QPTGPLLGGKADAVAAAHIAMETVVALLACALTPEKLPASLGGTSSGITGQLIRTIVDTI 230
Query: 238 AQSYNCCVVPGSRIRRIKRFLSGQNENIIVERDFKGVIWTESHQEAAMLART--VNETAV 295
A+SYNC VVPGSR+RRI+RFL+GQNE I+ ER++KGV+WTESHQEA +L+ T + T V
Sbjct: 231 ARSYNCGVVPGSRVRRIRRFLAGQNEGIVAEREYKGVVWTESHQEADLLSNTDAKDLTVV 290
Query: 296 DVKRKNPFIXXXXXXXXXXXXXXKGEVYMIDLKLAPLQG-LEPGLITLHTVDQFSGKS-E 353
D + PF GEVY+IDLK+A L+ + GL+TL TVD ++GKS +
Sbjct: 291 DRGQSTPF-TNVSAIPSDDFVVQSGEVYLIDLKMASLEHCTKKGLVTLETVDSYTGKSHK 349
Query: 354 SSDVVARPGAICRDFSKSHVLKLRAARQVLTRLDKQGVYPTKLAHIADSFP-LDVQEPDF 412
+ +++ARPGA RDF+++H+LKL+ +RQ+LT++DKQGVYP KL+H++ +FP + E +
Sbjct: 350 AGELIARPGAYVRDFAQTHILKLKTSRQLLTKIDKQGVYPFKLSHLSSNFPFVHENEEEL 409
Query: 413 ETIKQEIKPFRLGLSEISNNYLTIDRDVTIVKHVPWAKILDLTNPSGVSSYDAMAKNKGL 472
+++K+++K FRLG+SEISNNYL ++ + I + VPW IL TNP+G SYDA +
Sbjct: 410 QSLKKDLKSFRLGMSEISNNYLCVESPIQIARWVPWDHILKATNPNGNLSYDATSTL--T 467
Query: 473 YPGFELPLPXXXXXXXXXXXXXXXXXTPV--PVVRQCVTVAICDE--------ELLRISG 522
PG ELPLP + PV R+C T+ +CD ELLR++G
Sbjct: 468 LPGHELPLPKLGVSAIKLKSLMNSTKESISLPVARECNTIVLCDSSVSTTDRPELLRLTG 527
Query: 523 NLKS---NWVHSVYSMDTASNIVAGIFKLAQLSEDKRFGLKIRETQPWKMK 570
K+ +W+HS + ++ +IV GIF+LA L++DKRFGL ++ETQP K K
Sbjct: 528 GSKTCQPSWIHSQHELNPQDSIVQGIFQLATLAKDKRFGLLLKETQPMKQK 578
>SPBC23E6.05 [R] KOG2776 Metallopeptidase
Length = 417
Score = 102 bits (253), Expect = 2e-21
Identities = 103/423 (24%), Positives = 169/423 (39%), Gaps = 98/423 (23%)
Query: 27 KYRTAGQIAQTALNFLISLINKMYHEKSMEPLP---IHQLCLLTDSFIADCLSTKFNNKV 83
KYR AG +L++K +H+ + +P ++ D+ + + S+ + ++
Sbjct: 20 KYRDAG-----------ALVSKAFHQVASRCVPGASTREISSYGDNLLHEYKSSIYKSQR 68
Query: 84 NEKGISMPTCIDIDEINENWCPEVDDIENLSRWNQKSRSTKSIELCNGVTSSLSGFLKVG 143
EKGI+ PT I ++ N+ P + + + S L+VG
Sbjct: 69 FEKGIAEPTSICVNNCAYNYAPGPESVI--------------------AGNDNSYHLQVG 108
Query: 144 DVAKITLGCHIDGYTCNVSHTVVIYPTVQDAQSGKLLPEXXXXXXXXXXXXXTHIAVESV 203
DV KI++G H DGYT +SHT+V+ P Q + P H A ++V
Sbjct: 109 DVTKISMGLHFDGYTALISHTIVVTPPPQPGMGPYIGP-------GADAICAAHYASKAV 161
Query: 204 TNLLAAANDLTKLPADFNATKVTGSLIRTVVDTIAQSYNCCVVPGSRIRRIKRFLSGQNE 263
NLLA N + +TGS +R +VD IA + V PGSRIRRI RFL GQ
Sbjct: 162 ANLLATNN---------SDDPITGSRLRKIVDDIASQFRVSVCPGSRIRRISRFLVGQPT 212
Query: 264 NIIVERDFK---GVIWTESHQEAAMLARTVNETAVDVKRKNPFIXXXXXXXXXXXXXXKG 320
+E D V W +E T + +V
Sbjct: 213 IDRLEEDQNTKHAVEWPAPEEETRKADVTNSLDPANV----------LSTELNTWHVMPK 262
Query: 321 EVYMIDLKLAPLQGLEPGLITLHTVDQFSGKSESSDVVARPGAICRDFSKSHVLKLRAAR 380
E ++ID+ ++ S E D+ +P D + S++LKL+A+R
Sbjct: 263 EAWLIDISMSS--------------QPISSLKEHPDL--KPTLYIHDVNVSYMLKLKASR 306
Query: 381 QVLTRLDKQ-GVYPTKLAHIADSFPLDVQEPDFETIKQEIKPFRLGLSEISNNYLTIDRD 439
+L+ + K+ V+P ++ L LGL E+++ ++ +
Sbjct: 307 SLLSEIKKEKSVFPFHFGSLSSERNL------------------LGLRELTDRHILVPMP 348
Query: 440 VTI 442
V I
Sbjct: 349 VLI 351
>SPAC23H4.09 [R] KOG2776 Metallopeptidase
Length = 381
Score = 65.9 bits (159), Expect = 2e-10
Identities = 88/397 (22%), Positives = 148/397 (37%), Gaps = 115/397 (28%)
Query: 6 SKEDTQVLLKDKNVLIEPTIEKYRTAGQIAQTALNFLISLINKMYHEKSMEPLPIHQLCL 65
+KE T D ++ T+ KY+ AG+++Q + ++ L I+ +C+
Sbjct: 3 TKEATSETAVDYSLSNPETVNKYKIAGEVSQNVIKKVVELCQP--------GAKIYDICV 54
Query: 66 LTDSFIADCLSTKFNNKVNEKGISMPTCIDIDEINENWCPEVDDIE-NLSRWNQKSRSTK 124
D + + + + K KGI+ PT + +++ + P D E NL+
Sbjct: 55 RGDELLNEAIKKVYRTKDAYKGIAFPTAVSPNDMAAHLSPLKSDPEANLA---------- 104
Query: 125 SIELCNGVTSSLSGFLKVGDVAKITLGCHIDGYTCNVSHTVVIYPTVQDAQSGKLLPEXX 184
LK GDV KI LG HIDG+ V+ T V+ ++ +G
Sbjct: 105 ---------------LKSGDVVKILLGAHIDGFASLVATTTVV---SEEPVTG------- 139
Query: 185 XXXXXXXXXXXTHIAVESVTNLLAAANDLTKLPADFNATKVTGSLIRTVVDTIAQSYNCC 244
+++AAA+ K T + +VD IA SY C
Sbjct: 140 -----------------PAADVIAAASAALKAAQRTIKPGNTNWQVTDIVDKIATSYGCK 182
Query: 245 VVPGSRIRRIKRFLSGQNENIIVERDFKGVIWTESHQEAAMLARTVNETAVDVKRKNPFI 304
V G LS Q E +++ + ++ Q + M T E
Sbjct: 183 PVAG--------MLSHQQEREVIDGKKQVILNPSDSQRSEMDTFTFEE------------ 222
Query: 305 XXXXXXXXXXXXXXKGEVYMIDLKLAPLQGLEPGLITLHTVDQFSGKSESSDVVARPGAI 364
GEVY +D+ L P SGK + SD+ R I
Sbjct: 223 ---------------GEVYGVDI----LVSTSP-----------SGKVKRSDIATR---I 249
Query: 365 CRDFSKSHVLKLRAARQVLTRLD-KQGVYPTKLAHIA 400
+ +++LKL+A+R+V + + K G +P +I+
Sbjct: 250 YKKTDTTYMLKLQASRKVYSEIQTKFGPFPFSTRNIS 286
>Hs5453842 [R] KOG2776 Metallopeptidase
Length = 394
Score = 58.9 bits (141), Expect = 2e-08
Identities = 59/263 (22%), Positives = 99/263 (37%), Gaps = 62/263 (23%)
Query: 5 ISKEDTQVLLKDKNVLIEPTIEKYRTAGQIAQTALNFLISLINKMYHEKSMEPLPIHQLC 64
+S ED Q +++ + + + KY+ G IA L L+ E S + + LC
Sbjct: 1 MSGEDEQ---QEQTIAEDLVVTKYKMGGDIANRVLRSLV--------EASSSGVSVLSLC 49
Query: 65 LLTDSFIADCLSTKFNN-KVNEKGISMPTCIDIDEINENWCPEVDDIENLSRWNQKSRST 123
D+ I + F K +KGI+ PT I ++ ++ P D + +
Sbjct: 50 EKGDAMIMEETGKIFKKEKEMKKGIAFPTSISVNNCVCHFSPLKSDQDYI---------- 99
Query: 124 KSIELCNGVTSSLSGFLKVGDVAKITLGCHIDGYTCNVSHTVVIYPTVQDAQSGKLLPEX 183
LK GD+ KI LG H+DG+ NV+HT V+ D G
Sbjct: 100 ----------------LKEGDLVKIDLGVHVDGFIANVAHTFVV-----DVAQG-----T 133
Query: 184 XXXXXXXXXXXXTHIAVESVTNLLAAANDLTKLPADFNATKVTGSLIRTVVDTIAQSYNC 243
H+ E+ L+ N T++ +N +A S+NC
Sbjct: 134 QVTGRKADVIKAAHLCAEAALRLVKPGNQNTQVTEAWN--------------KVAHSFNC 179
Query: 244 CVVPGSRIRRIKRFLSGQNENII 266
+ G ++K+ + + II
Sbjct: 180 TPIEGMLSHQLKQHVIDGEKTII 202
>Hs20553033 [R] KOG2776 Metallopeptidase
Length = 210
Score = 58.9 bits (141), Expect = 2e-08
Identities = 59/263 (22%), Positives = 99/263 (37%), Gaps = 62/263 (23%)
Query: 5 ISKEDTQVLLKDKNVLIEPTIEKYRTAGQIAQTALNFLISLINKMYHEKSMEPLPIHQLC 64
+S ED Q +++ + + + KY+ G IA L L+ E S + + LC
Sbjct: 1 MSGEDEQ---QEQTIAEDLVVTKYKMGGDIANRVLRSLV--------EASSSGVSVLSLC 49
Query: 65 LLTDSFIADCLSTKFNN-KVNEKGISMPTCIDIDEINENWCPEVDDIENLSRWNQKSRST 123
D+ I + F K +KGI+ PT I ++ ++ P D + +
Sbjct: 50 EKGDAMIMEETGKIFKKEKEMKKGIAFPTSISVNNCVCHFSPLKSDQDYI---------- 99
Query: 124 KSIELCNGVTSSLSGFLKVGDVAKITLGCHIDGYTCNVSHTVVIYPTVQDAQSGKLLPEX 183
LK GD+ KI LG H+DG+ NV+HT V+ D G
Sbjct: 100 ----------------LKEGDLVKIDLGVHVDGFIANVAHTFVV-----DVAQG-----T 133
Query: 184 XXXXXXXXXXXXTHIAVESVTNLLAAANDLTKLPADFNATKVTGSLIRTVVDTIAQSYNC 243
H+ E+ L+ N T++ +N +A S+NC
Sbjct: 134 QVTGRKADVIKAAHLCAEAALRLVKPGNQNTQVTEAWN--------------KVAHSFNC 179
Query: 244 CVVPGSRIRRIKRFLSGQNENII 266
+ G ++K+ + + II
Sbjct: 180 TPIEGMLSHQLKQHVIDGEKTII 202
>CE21275 [R] KOG2776 Metallopeptidase
Length = 391
Score = 54.3 bits (129), Expect = 5e-07
Identities = 53/219 (24%), Positives = 81/219 (36%), Gaps = 44/219 (20%)
Query: 15 KDKNVLIEPTIEKYRTAGQIAQTALNFLISLINKMYHEKSMEPLPIHQLCLLTDSFIADC 74
+D + + + KY+ A +I L +++ I E LC L D I +
Sbjct: 19 EDYTLANDAVVTKYQVAAEITNAVLKEVLANIK--------EGAIAGDLCDLGDKLILEK 70
Query: 75 LSTKFNNKVN-EKGISMPTCIDIDEINENWCPEVDDIENLSRWNQKSRSTKSIELCNGVT 133
+ + N KGI+MPTCI ID ++ P +
Sbjct: 71 TGKLYKKEKNFTKGIAMPTCISIDNCICHYTP--------------------------LK 104
Query: 134 SSLSGFLKVGDVAKITLGCHIDGYTCNVSHTVVIYPTVQDAQSGKLLPEXXXXXXXXXXX 193
S LK G V K+ LG HIDG +HTVV+ + + +GKL
Sbjct: 105 SEAPVVLKNGQVVKVDLGTHIDGLIATAAHTVVVGASKDNKVTGKL------ADLLRGTH 158
Query: 194 XXTHIAVESV---TNLLAAANDLTKLPADFNATKVTGSL 229
IA+ S+ T ++ K A+F T + L
Sbjct: 159 DALEIAIRSLRPDTENTTITKNIDKTAAEFGLTPIENML 197
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,353,602
Number of Sequences: 60738
Number of extensions: 1183318
Number of successful extensions: 2753
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 2735
Number of HSP's gapped (non-prelim): 10
length of query: 571
length of database: 30,389,216
effective HSP length: 112
effective length of query: 459
effective length of database: 23,586,560
effective search space: 10826231040
effective search space used: 10826231040
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)