ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI3133 good C KOG0225 Energy production and conversion Pyruvate dehydrogenase E1, alpha subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI3133 1101429 1102664 412
(412 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YER178w [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit 681 0.0
SPAC26F1.03 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit 468 e-132
CE01643 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit 375 e-104
At1g24180 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit 370 e-102
At1g59900 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit 367 e-101
Hs4505685 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit 359 5e-99
Hs4885543 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit 354 1e-97
7290527 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit 354 2e-97
7290525 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit 354 2e-97
ECU09g1040 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit 251 1e-66
At1g01090 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit 224 2e-58
Hs11386135 [C] KOG1182 Branched chain alpha-keto acid dehydrogen... 143 5e-34
7299201 [C] KOG1182 Branched chain alpha-keto acid dehydrogenase... 137 3e-32
At1g21400 [C] KOG1182 Branched chain alpha-keto acid dehydrogena... 136 5e-32
At5g09300 [C] KOG1182 Branched chain alpha-keto acid dehydrogena... 129 8e-30
CE16618 [C] KOG1182 Branched chain alpha-keto acid dehydrogenase... 114 3e-25
At5g34780_1 [C] KOG1182 Branched chain alpha-keto acid dehydroge... 96 1e-19
At5g65750 [G] KOG0450 2-oxoglutarate dehydrogenase E1 subunit 50 6e-06
>YER178w [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit
Length = 443
Score = 681 bits (1757), Expect = 0.0
Identities = 328/387 (84%), Positives = 358/387 (91%), Gaps = 3/387 (0%)
Query: 29 ALSQVADETKPGD---DDLVQIDLPETSFEGYLLDVPELSYQTTKSNLLQMYKDMIIVRR 85
A + D+ P D D VQI+LPE+SFE Y+L+ P+LSY+T+K+ LLQMYKDM+I+RR
Sbjct: 57 ATLKTTDKKAPEDIEGSDTVQIELPESSFESYMLEPPDLSYETSKATLLQMYKDMVIIRR 116
Query: 86 MEMACDALYKAKKIRGFCHSSVGQEAIAVGIENAITKRDTVITSYRCHGFTYMRGAAVQA 145
MEMACDALYKAKKIRGFCH SVGQEAIAVGIENAITK D++ITSYRCHGFT+MRGA+V+A
Sbjct: 117 MEMACDALYKAKKIRGFCHLSVGQEAIAVGIENAITKLDSIITSYRCHGFTFMRGASVKA 176
Query: 146 VLAELMGRRTGVSFGKGGSMHLYAPGFYGGNGIVGAQVPLGAGLAFAHQYKHEDACSFAL 205
VLAELMGRR GVS+GKGGSMHLYAPGFYGGNGIVGAQVPLGAGLAFAHQYK+EDACSF L
Sbjct: 177 VLAELMGRRAGVSYGKGGSMHLYAPGFYGGNGIVGAQVPLGAGLAFAHQYKNEDACSFTL 236
Query: 206 YGDGASNQGQVFESFNMAKLWNLPAVFCCENNKYGMGTAAARSSAMTEYFKRGQYIPGLK 265
YGDGASNQGQVFESFNMAKLWNLP VFCCENNKYGMGTAA+RSSAMTEYFKRGQYIPGLK
Sbjct: 237 YGDGASNQGQVFESFNMAKLWNLPVVFCCENNKYGMGTAASRSSAMTEYFKRGQYIPGLK 296
Query: 266 VNGMDILAVYQASKFAKDWTVSGNGPIVLEYETYRYGGHSMSDPGTTYRTRDEIQHMRSK 325
VNGMDILAVYQASKFAKDW +SG GP+VLEYETYRYGGHSMSDPGTTYRTRDEIQHMRSK
Sbjct: 297 VNGMDILAVYQASKFAKDWCLSGKGPLVLEYETYRYGGHSMSDPGTTYRTRDEIQHMRSK 356
Query: 326 NDPIAGLKMHLLELGIATEDEIKAYDKAARKYVDEQVELADAAPAPEAKMSILFEDVYVP 385
NDPIAGLKMHL++LGIATE E+KAYDK+ARKYVDEQVELADAAP PEAK+SILFEDVYV
Sbjct: 357 NDPIAGLKMHLIDLGIATEAEVKAYDKSARKYVDEQVELADAAPPPEAKLSILFEDVYVK 416
Query: 386 GSETPTLRGRLQEDTWDFAKKSFAFRD 412
G+ETPTLRGR+ EDTWDF K+ FA RD
Sbjct: 417 GTETPTLRGRIPEDTWDFKKQGFASRD 443
>SPAC26F1.03 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit
Length = 409
Score = 468 bits (1205), Expect = e-132
Identities = 216/349 (61%), Positives = 274/349 (77%)
Query: 47 IDLPETSFEGYLLDVPELSYQTTKSNLLQMYKDMIIVRRMEMACDALYKAKKIRGFCHSS 106
+ L ++ FEGY +DVP + TK LL +Y+ M+ +RR+E+ACDALYKAKKIRGFCH S
Sbjct: 52 VKLDDSVFEGYKIDVPSTEIEVTKGELLGLYEKMVTIRRLELACDALYKAKKIRGFCHLS 111
Query: 107 VGQEAIAVGIENAITKRDTVITSYRCHGFTYMRGAAVQAVLAELMGRRTGVSFGKGGSMH 166
+GQEA+A GIE AIT D++ITSYRCHGF Y RG ++++++ ELMGR+ G S GKGGSMH
Sbjct: 112 IGQEAVAAGIEGAITLDDSIITSYRCHGFAYTRGLSIRSIIGELMGRQCGASKGKGGSMH 171
Query: 167 LYAPGFYGGNGIVGAQVPLGAGLAFAHQYKHEDACSFALYGDGASNQGQVFESFNMAKLW 226
++A FYGGNGIVGAQ+PLGAG+ FA +Y + +FALYGDGASNQGQ FE+FNMAKLW
Sbjct: 172 IFAKNFYGGNGIVGAQIPLGAGIGFAQKYLEKPTTTFALYGDGASNQGQAFEAFNMAKLW 231
Query: 227 NLPAVFCCENNKYGMGTAAARSSAMTEYFKRGQYIPGLKVNGMDILAVYQASKFAKDWTV 286
LP +F CENNKYGMGT+A RSSAMTE++KRGQYIPGL VNGMD+LAV QASKFAK +TV
Sbjct: 232 GLPVIFACENNKYGMGTSAERSSAMTEFYKRGQYIPGLLVNGMDVLAVLQASKFAKKYTV 291
Query: 287 SGNGPIVLEYETYRYGGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDE 346
+ P+++E+ TYRYGGHSMSDPGTTYR+R+E+Q +R+ DPI GLK H++E G+A +E
Sbjct: 292 ENSQPLLMEFVTYRYGGHSMSDPGTTYRSREEVQKVRAARDPIEGLKKHIMEWGVANANE 351
Query: 347 IKAYDKAARKYVDEQVELADAAPAPEAKMSILFEDVYVPGSETPTLRGR 395
+K +K R VDE+V +A+ +P P+ LF DVYV G+E RGR
Sbjct: 352 LKNIEKRIRGMVDEEVRIAEESPFPDPIEESLFSDVYVAGTEPAYARGR 400
>CE01643 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit
Length = 397
Score = 375 bits (963), Expect = e-104
Identities = 190/343 (55%), Positives = 243/343 (70%), Gaps = 11/343 (3%)
Query: 62 PELSYQTTKSNLLQMYKDMIIVRRMEMACDALYKAKKIRGFCHSSVGQEAIAVGIENAIT 121
P S + + L+ Y+DM ++RRME A LYK KKIRGFCH GQEA AVG++ A+T
Sbjct: 44 PNTSVTLNREDALKYYRDMQVIRRMESAAGNLYKEKKIRGFCHLYSGQEACAVGMKAAMT 103
Query: 122 KRDTVITSYRCHGFTYMRGAAVQAVLAELMGRRTGVSFGKGGSMHLYAPGFYGGNGIVGA 181
+ D VIT+YRCHG+T++ GA V VLAEL GR G GKGGSMH+Y FYGGNGIVGA
Sbjct: 104 EGDAVITAYRCHGWTWLLGATVTEVLAELTGRVAGNVHGKGGSMHMYTKNFYGGNGIVGA 163
Query: 182 QVPLGAGLAFAHQYKHEDACSFALYGDGASNQGQVFESFNMAKLWNLPAVFCCENNKYGM 241
Q PLGAG+A A +Y+ + LYGDGA+NQGQ+FE+ NMAKLW+LP +F CENN +GM
Sbjct: 164 QQPLGAGVALAMKYREQKNVCVTLYGDGAANQGQLFEATNMAKLWDLPVLFVCENNGFGM 223
Query: 242 GTAAARSSAMTEYFKRGQYIPGLKVNGMDILAVYQASKFAKDWTVSGNGPIVLEYETYRY 301
GT A RSSA TEY+ RG Y+PG+ V+GMDILAV +A+K+AK++ SG GP+++E TYRY
Sbjct: 224 GTTAERSSASTEYYTRGDYVPGIWVDGMDILAVREATKWAKEYCDSGKGPLMMEMATYRY 283
Query: 302 GGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDEIKAYDKAARKYVDEQ 361
GHSMSDPGT+YRTR+EIQ +R DPI G K ++ +ATE+E+KA DK RK VDE
Sbjct: 284 HGHSMSDPGTSYRTREEIQEVRKTRDPITGFKDRIITSSLATEEELKAIDKEVRKEVDEA 343
Query: 362 VELA--DAAPAPEAKMSILFEDVY--VPGSETPTLRGRLQEDT 400
+++A D PEA L+ D+Y P E +RG ++T
Sbjct: 344 LKIATSDGVLPPEA----LYADIYHNTPAQE---IRGATIDET 379
>At1g24180 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit
Length = 393
Score = 370 bits (949), Expect = e-102
Identities = 190/333 (57%), Positives = 238/333 (71%), Gaps = 5/333 (1%)
Query: 54 FEGYLLDVPELSYQTTKSNLLQMYKDMIIVRRMEMACDALYKAKKIRGFCHSSVGQEAIA 113
F +L + P S +T+ +L ++DM +RRME+A D+LYKAK IRGFCH GQEA+A
Sbjct: 44 FTSHLCESPSRSVETSSEEILAFFRDMARMRRMEIAADSLYKAKLIRGFCHLYDGQEALA 103
Query: 114 VGIENAITKRDTVITSYRCHGFTYMRGAAVQAVLAELMGRRTGVSFGKGGSMHLYAP--G 171
VG+E AITK+D +ITSYR H RG + +ELMGR+TG S GKGGSMH Y
Sbjct: 104 VGMEAAITKKDAIITSYRDHCTFIGRGGKLVDAFSELMGRKTGCSHGKGGSMHFYKKDAS 163
Query: 172 FYGGNGIVGAQVPLGAGLAFAHQYKHEDACSFALYGDGASNQGQVFESFNMAKLWNLPAV 231
FYGG+GIVGAQ+PLG GLAFA +Y ++A +FALYGDGA+NQGQ+FE+ N++ LW+LPA+
Sbjct: 164 FYGGHGIVGAQIPLGCGLAFAQKYNKDEAVTFALYGDGAANQGQLFEALNISALWDLPAI 223
Query: 232 FCCENNKYGMGTAAARSSAMTEYFKRGQYIPGLKVNGMDILAVYQASKFAKDWTVSGNGP 291
CENN YGMGTA RS+ YFKRG Y+PGLKV+GMD LAV QA KFAK+ + NGP
Sbjct: 224 LVCENNHYGMGTATWRSAKSPAYFKRGDYVPGLKVDGMDALAVKQACKFAKEHALK-NGP 282
Query: 292 IVLEYETYRYGGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDEIKAYD 351
I+LE +TYRY GHSMSDPG+TYRTRDEI +R DPI ++ LL IATE E+K +
Sbjct: 283 IILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQVRDPIERVRKLLLTHDIATEKELKDME 342
Query: 352 KAARKYVDEQVELADAAPAPEAKMSILFEDVYV 384
K RK VD+ V A +P P+A S LF ++YV
Sbjct: 343 KEIRKEVDDAVAQAKESPIPDA--SELFTNMYV 373
>At1g59900 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit
Length = 389
Score = 367 bits (942), Expect = e-101
Identities = 189/343 (55%), Positives = 240/343 (69%), Gaps = 7/343 (2%)
Query: 51 ETS--FEGYLLDVPELSYQTTKSNLLQMYKDMIIVRRMEMACDALYKAKKIRGFCHSSVG 108
ETS F +L D P S +++ LL ++ M ++RRME+A D+LYKAK IRGFCH G
Sbjct: 35 ETSLPFTAHLCDPPSRSVESSSQELLDFFRTMALMRRMEIAADSLYKAKLIRGFCHLYDG 94
Query: 109 QEAIAVGIENAITKRDTVITSYRCHGFTYMRGAAVQAVLAELMGRRTGVSFGKGGSMHLY 168
QEA+A+G+E AITK+D +IT+YR H RG ++ V +ELMGR+ G S GKGGSMH Y
Sbjct: 95 QEAVAIGMEAAITKKDAIITAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFY 154
Query: 169 AP--GFYGGNGIVGAQVPLGAGLAFAHQYKHEDACSFALYGDGASNQGQVFESFNMAKLW 226
FYGG+GIVGAQVPLG G+AFA +Y E+A +FALYGDGA+NQGQ+FE+ N++ LW
Sbjct: 155 KKESSFYGGHGIVGAQVPLGCGIAFAQKYNKEEAVTFALYGDGAANQGQLFEALNISALW 214
Query: 227 NLPAVFCCENNKYGMGTAAARSSAMTEYFKRGQYIPGLKVNGMDILAVYQASKFAKDWTV 286
+LPA+ CENN YGMGTA R++ Y+KRG Y+PGLKV+GMD AV QA KFAK +
Sbjct: 215 DLPAILVCENNHYGMGTAEWRAAKSPSYYKRGDYVPGLKVDGMDAFAVKQACKFAKQHAL 274
Query: 287 SGNGPIVLEYETYRYGGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDE 346
GPI+LE +TYRY GHSMSDPG+TYRTRDEI +R + DPI +K +L +ATE E
Sbjct: 275 E-KGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLATEKE 333
Query: 347 IKAYDKAARKYVDEQVELADAAPAPEAKMSILFEDVYVPGSET 389
+K +K RK VD+ + A P PE S LF +VYV G T
Sbjct: 334 LKDMEKEIRKEVDDAIAKAKDCPMPEP--SELFTNVYVKGFGT 374
>Hs4505685 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit
Length = 390
Score = 359 bits (921), Expect = 5e-99
Identities = 171/310 (55%), Positives = 226/310 (72%)
Query: 62 PELSYQTTKSNLLQMYKDMIIVRRMEMACDALYKAKKIRGFCHSSVGQEAIAVGIENAIT 121
P ++ T+ + L+ Y+ M VRRME+ D LYK K IRGFCH GQEA VG+E I
Sbjct: 50 PPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGIN 109
Query: 122 KRDTVITSYRCHGFTYMRGAAVQAVLAELMGRRTGVSFGKGGSMHLYAPGFYGGNGIVGA 181
D +IT+YR HGFT+ RG +V+ +LAEL GR+ G + GKGGSMH+YA FYGGNGIVGA
Sbjct: 110 PTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGA 169
Query: 182 QVPLGAGLAFAHQYKHEDACSFALYGDGASNQGQVFESFNMAKLWNLPAVFCCENNKYGM 241
QVPLGAG+A A +Y +D LYGDGA+NQGQ+FE++NMA LW LP +F CENN+YGM
Sbjct: 170 QVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGM 229
Query: 242 GTAAARSSAMTEYFKRGQYIPGLKVNGMDILAVYQASKFAKDWTVSGNGPIVLEYETYRY 301
GT+ R++A T+Y+KRG +IPGL+V+GMDIL V +A++FA + SG GPI++E +TYRY
Sbjct: 230 GTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRY 289
Query: 302 GGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDEIKAYDKAARKYVDEQ 361
GHSMSDPG +YRTR+EIQ +RSK+DPI LK ++ +A+ +E+K D RK +++
Sbjct: 290 HGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDA 349
Query: 362 VELADAAPAP 371
+ A A P P
Sbjct: 350 AQFATADPEP 359
>Hs4885543 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit
Length = 388
Score = 354 bits (909), Expect = 1e-97
Identities = 175/316 (55%), Positives = 223/316 (70%), Gaps = 1/316 (0%)
Query: 57 YLLDV-PELSYQTTKSNLLQMYKDMIIVRRMEMACDALYKAKKIRGFCHSSVGQEAIAVG 115
YLL+ P ++ T++ L+ Y+ M+ VRRME+ D LYK K IRGFCH GQEA VG
Sbjct: 42 YLLEEGPPVTTVLTRAEGLKYYRMMLTVRRMELKADQLYKQKFIRGFCHLCDGQEACCVG 101
Query: 116 IENAITKRDTVITSYRCHGFTYMRGAAVQAVLAELMGRRTGVSFGKGGSMHLYAPGFYGG 175
+E I D VITSYR HG Y RG +V+++LAEL GRR G + GKGGSMH+Y FYGG
Sbjct: 102 LEAGINPSDHVITSYRAHGVCYTRGLSVRSILAELTGRRGGCAKGKGGSMHMYTKNFYGG 161
Query: 176 NGIVGAQVPLGAGLAFAHQYKHEDACSFALYGDGASNQGQVFESFNMAKLWNLPAVFCCE 235
NGIVGAQ PLGAG+A A +YK D LYGDGA+NQGQ+ E+FNMA LW LP VF CE
Sbjct: 162 NGIVGAQGPLGAGIALACKYKGNDEICLTLYGDGAANQGQIAEAFNMAALWKLPCVFICE 221
Query: 236 NNKYGMGTAAARSSAMTEYFKRGQYIPGLKVNGMDILAVYQASKFAKDWTVSGNGPIVLE 295
NN YGMGT+ R++A +Y+KRG +IPGLKV+GMD+L V +A+KFA ++ SG GPI++E
Sbjct: 222 NNLYGMGTSTERAAASPDYYKRGNFIPGLKVDGMDVLCVREATKFAANYCRSGKGPILME 281
Query: 296 YETYRYGGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDEIKAYDKAAR 355
+TYRY GHSMSDPG +YRTR+EIQ +RSK DPI L+ ++ +AT +E+K R
Sbjct: 282 LQTYRYHGHSMSDPGVSYRTREEIQEVRSKRDPIIILQDRMVNSKLATVEELKEIGAEVR 341
Query: 356 KYVDEQVELADAAPAP 371
K +D+ + A P P
Sbjct: 342 KEIDDAAQFATTDPEP 357
>7290527 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit
Length = 479
Score = 354 bits (908), Expect = 2e-97
Identities = 176/334 (52%), Positives = 231/334 (68%), Gaps = 4/334 (1%)
Query: 51 ETSFEGYLLDV-PELSYQTTKSNLLQMYKDMIIVRRMEMACDALYKAKKIRGFCHSSVGQ 109
E +F+ Y L+ P + + ++ + L MY M+ +RR E YK +KIRGFCH GQ
Sbjct: 40 ENTFKCYDLENGPTMDVELSREDALTMYTQMLELRRFETVAGNYYKERKIRGFCHLYNGQ 99
Query: 110 EAIAVGIENAITKRDTVITSYRCHGFTYMRGAAVQAVLAELMGRRTGVSFGKGGSMHLYA 169
EA+AVG++ + D+VIT+YRCH +TY+ G ++ ++AEL G RTG S GKGGSMH+Y+
Sbjct: 100 EAVAVGMKQRLRSCDSVITAYRCHAWTYLMGVSLYEIMAELFGVRTGCSRGKGGSMHMYS 159
Query: 170 PGFYGGNGIVGAQVPLGAGLAFAHQYKHEDACSFALYGDGASNQGQVFESFNMAKLWNLP 229
FYGGNGIVGAQVPLGAG+ AH Y+ ++ S LYGDGA+NQGQ+FESFNMAKLW LP
Sbjct: 160 DKFYGGNGIVGAQVPLGAGIGLAHSYRKDNGVSVVLYGDGAANQGQIFESFNMAKLWCLP 219
Query: 230 AVFCCENNKYGMGTAAARSSAMTEYFKRGQYIPGLKVNGMDILAVYQASKFAKDWTVSGN 289
+F CENN YGMGT R+SAMTE++ RGQYIPGL V+G +LAV A++FA D + +
Sbjct: 220 CIFVCENNHYGMGTHVKRASAMTEFYMRGQYIPGLWVDGNQVLAVRSATQFAVDHALK-H 278
Query: 290 GPIVLEYETYRYGGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDEIKA 349
GPIVLE TYRY GHSMSDPGT+YR+R+E+Q R K DPI + ++ L +A E+E+KA
Sbjct: 279 GPIVLEMSTYRYVGHSMSDPGTSYRSREEVQSTREKRDPITSFRSQIIALCLADEEELKA 338
Query: 350 YDKAARKYVDEQVELADAAPAPEAKMSILFEDVY 383
D RK VD + A E ++ L D+Y
Sbjct: 339 LDDKTRKQVDSICK--KATTDREVELDELHTDIY 370
>7290525 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit
Length = 443
Score = 354 bits (908), Expect = 2e-97
Identities = 196/395 (49%), Positives = 256/395 (64%), Gaps = 16/395 (4%)
Query: 14 SSSLRLVRN--FSKNVRALSQ------VADETKPGDDDLVQIDLPETSFEGYLLDV-PEL 64
+SS+ ++RN +S + L Q V+ + VQ++ P F+ + LD P
Sbjct: 44 NSSIDIIRNGRYSSSFFGLFQNAAQAGVSKTNNYATEATVQVNRP---FKLHRLDEGPAT 100
Query: 65 SYQTTKSNLLQMYKDMIIVRRMEMACDALYKAKKIRGFCHSSVGQEAIAVGIENAITKRD 124
+ TK L+ Y M +RR+E A LYK K IRGFCH GQEA AVG++ A+ D
Sbjct: 101 EVKLTKDQALKYYTQMQTIRRLETAAGNLYKEKIIRGFCHLYSGQEACAVGMKAAMRDVD 160
Query: 125 TVITSYRCHGFTYMRGAAVQAVLAELMGRRTGVSFGKGGSMHLYAPGFYGGNGIVGAQVP 184
+I++YR HG+TY+ G + VLAEL G + G + GKGGSMH+YAP FYGGNGIVGAQVP
Sbjct: 161 NIISAYRVHGWTYLMGVSPSGVLAELTGVQGGCARGKGGSMHMYAPNFYGGNGIVGAQVP 220
Query: 185 LGAGLAFAHQYKHEDACSFALYGDGASNQGQVFESFNMAKLWNLPAVFCCENNKYGMGTA 244
LGAG+ A +YK ALYGDGA+NQGQVFE++NMA LW LP +F CENN YGMGT+
Sbjct: 221 LGAGVGLACKYKGNGGMCLALYGDGAANQGQVFEAYNMAYLWKLPVIFVCENNNYGMGTS 280
Query: 245 AARSSAMTEYFKRGQYIPGLKVNGMDILAVYQASKFAKDWTVSGNGPIVLEYETYRYGGH 304
+ R+S T+Y+ RG +PG+ V+GMD+LAV A++FA ++ V+ +GP+V+E TYRY GH
Sbjct: 281 SERASCNTDYYTRGDALPGIWVDGMDVLAVRSATEFAINY-VNTHGPLVMETNTYRYSGH 339
Query: 305 SMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDEIKAYDKAARKYVDEQVEL 364
SMSDPGT+YRTR+EIQ +R K DPI K +ELG+ T DE+KA D RK VDE
Sbjct: 340 SMSDPGTSYRTREEIQEVRQKRDPITSFKELCIELGLITTDEVKAIDLKVRKEVDEAT-- 397
Query: 365 ADAAPAPEAKMSILFEDVYVPGSETPTLRGRLQED 399
A A E +S L+ DVY E P LRG + D
Sbjct: 398 AFAKSDAELGVSHLWTDVYSNNLE-PKLRGTIAYD 431
>ECU09g1040 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit
Length = 349
Score = 251 bits (641), Expect = 1e-66
Identities = 130/296 (43%), Positives = 188/296 (62%), Gaps = 11/296 (3%)
Query: 76 MYKDMIIVRRMEMACDALYKAKKIRGFCHSSVGQEAIAVGIENAITKRDTVITSYRCHGF 135
+YK M+ +R M+ A D YK K IRGFCH S+GQE I +E A+ D ++SYRCHG
Sbjct: 42 IYKQMMRMRCMDEAMDREYKRKNIRGFCHLSIGQEGIYAALEYAMDG-DVAVSSYRCHGI 100
Query: 136 TYMRGAAVQAVLAELMGRRTGVSFGKGGSMHLYAPGFYGGNGIVGAQVPLGAGLAFAHQY 195
Y+ G ++ ++ E++GR+ GV GKGGSMHLY F+GG+GIVGAQ+PLG G+A+A +Y
Sbjct: 101 AYVTGCSILEIMGEVLGRQAGVCKGKGGSMHLYNKSFFGGHGIVGAQIPLGLGMAYALEY 160
Query: 196 KHEDACS------FALYGDGASNQGQVFESFNMAKLWNLPAVFCCENNKYGMGTAAARSS 249
S +A YGDGA+NQGQV+ESFNMA +W LP VF CENN YGM T A+ S
Sbjct: 161 NRRMGWSQGGKVCYAFYGDGAANQGQVWESFNMAMVWRLPIVFVCENNGYGMWTPASSVS 220
Query: 250 AMTEYFKRGQYIPGLKVNGMDILAVYQASKFAKDWTVSGNGPIVLEYETYRYGGHSMSDP 309
A T+++ RG IPG+++ +I + K+A+ ++V NGPI+++ +TYR+ HS +D
Sbjct: 221 ADTDFYLRGGAIPGIRIGHGNIFGLMSVLKYARKYSVE-NGPIIVQIDTYRFCTHSAADE 279
Query: 310 GTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDEIKAYDKAARKYVDEQVELA 365
+YR+R+E+ K D + + L L +E+E+ A + V+ V+ A
Sbjct: 280 RESYRSREEVD-AEKKRDCMEDVGRRL--LAFYSEEELDALRSSILAEVERDVDAA 332
>At1g01090 [C] KOG0225 Pyruvate dehydrogenase E1 alpha subunit
Length = 428
Score = 224 bits (570), Expect = 2e-58
Identities = 124/325 (38%), Positives = 192/325 (58%), Gaps = 14/325 (4%)
Query: 69 TKSNLLQMYKDMIIVRRMEMACDALYKAKKIRGFCHSSVGQEAIAVGIENAITKRDTVIT 128
TK L++Y+DMI+ R E C +Y K+ GF H GQEA++ G +TK D+V++
Sbjct: 80 TKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKSDSVVS 139
Query: 129 SYRCHGFTYMRGAAVQAVLAELMGRRTGVSFGKGGSMHLYAP--GFYGGNGIVGAQVPLG 186
+YR H +G + +AV++EL G+ TG G+GGSMH+++ GG +G +P+
Sbjct: 140 TYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEHNMLGGFAFIGEGIPVA 199
Query: 187 AGLAFAHQYKHE------DACSFALYGDGASNQGQVFESFNMAKLWNLPAVFCCENNKYG 240
G AF+ +Y+ E D + A +GDG N GQ FE NMA L+ LP +F ENN +
Sbjct: 200 TGAAFSSKYRREVLKQDCDDVTVAFFGDGTCNNGQFFECLNMAALYKLPIIFVVENNLWA 259
Query: 241 MGTAAARSSAMTEYFKRGQY--IPGLKVNGMDILAVYQASKFAKDWTVSGNGPIVLEYET 298
+G + R+++ E +K+G +PG+ V+GMD+L V + +K A G GP ++E ET
Sbjct: 260 IGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAVTRARRGEGPTLVECET 319
Query: 299 YRYGGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDEIKAYDKAARKYV 358
YR+ GHS++DP ++ ++ + DPIA LK +L+E +A E E+K+ +K + V
Sbjct: 320 YRFRGHSLADPDELRDAAEKAKY--AARDPIAALKKYLIENKLAKEAELKSIEKKIDELV 377
Query: 359 DEQVELADAAPAPEAKMSILFEDVY 383
+E VE ADA+P P S L E+V+
Sbjct: 378 EEAVEFADASPQP--GRSQLLENVF 400
>Hs11386135 [C] KOG1182 Branched chain alpha-keto acid dehydrogenase complex
alpha subunit
Length = 445
Score = 143 bits (360), Expect = 5e-34
Identities = 97/333 (29%), Positives = 159/333 (47%), Gaps = 10/333 (3%)
Query: 70 KSNLLQMYKDMIIVRRMEMACDALYKAKKIRGFCHSSVGQEAIAVGIENAITKRDTVITS 129
K +L++YK M ++ M+ + +I F ++ G+E VG A+ D V
Sbjct: 99 KEKVLKLYKSMTLLNTMDRILYESQRQGRI-SFYMTNYGEEGTHVGSAAALDNTDLVFGQ 157
Query: 130 YRCHGFTYMRGAAVQAVLAELMGRRTGVSFGKGGSMHLYAPG--FYGGNGIVGAQVPLGA 187
YR G R ++ +A+ G + + G+ +H F + + Q+P
Sbjct: 158 YREAGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLATQIPQAV 217
Query: 188 GLAFAHQYKHEDACSFALYGDGASNQGQVFESFNMAKLWNLPAVFCCENNKYGMGTAAAR 247
G A+A + + + +G+GA+++G FN A P +F C NN Y + T +
Sbjct: 218 GAAYAAKRANANRVVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSE 277
Query: 248 SSAMTEYFKRGQY--IPGLKVNGMDILAVYQASKFAKDWTVSGNGPIVLEYETYRYGGHS 305
RG I ++V+G D+ AVY A+K A+ V+ N P ++E TYR G HS
Sbjct: 278 QYRGDGIAARGPGYGIMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHS 337
Query: 306 MSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDEIKAYDKAARKYVDEQVELA 365
SD + YR+ DE+ + ++ PI+ L+ +LL G E++ KA+ K +R+ V E E A
Sbjct: 338 TSDDSSAYRSVDEVNYWDKQDHPISRLRHYLLSQGWWDEEQEKAWRKQSRRKVMEAFEQA 397
Query: 366 DAAPAPEAKMSILFEDVYVPGSETPTLRGRLQE 398
+ P P ++LF DVY E P + QE
Sbjct: 398 ERKPKPNP--NLLFSDVY---QEMPAQLRKQQE 425
>7299201 [C] KOG1182 Branched chain alpha-keto acid dehydrogenase complex
alpha subunit
Length = 439
Score = 137 bits (345), Expect = 3e-32
Identities = 93/335 (27%), Positives = 160/335 (47%), Gaps = 10/335 (2%)
Query: 55 EGYLLDVPELSYQTTKSNLLQMYKDMIIVRRMEMACDALYKAKKIRGFCHSSVGQEAIAV 114
+GY+ D + Q + + +M++DM+++ M+ + +I F ++ G+EA +
Sbjct: 77 DGYIADETQ-DPQLGREVVEKMFRDMVLLNTMDKILYESQRQGRI-SFYMTNFGEEASHI 134
Query: 115 GIENAITKRDTVITSYRCHGFTYMRGAAVQAVLAELMGRRTGVSFGKGGSMHLYAP--GF 172
G A+ RD + YR G RG + + + G + GK +H + F
Sbjct: 135 GSAAALEMRDLIYGQYREAGVLVWRGFRIDQFIDQCYGNTDDLGRGKQMPVHYGSRELNF 194
Query: 173 YGGNGIVGAQVPLGAGLAFAHQYK-HEDACSFALYGDGASNQGQVFESFNMAKLWNLPAV 231
+ + Q+P G A+A + + + DAC +G+GA+++G +FN A PA+
Sbjct: 195 VTISSPLSTQMPQAVGAAYAMKLRPNNDACVVCYFGEGAASEGDAHAAFNFAATLGCPAI 254
Query: 232 FCCENNKYGMGTAAA---RSSAMTEYFKRGQYIPGLKVNGMDILAVYQASKFAKDWTVSG 288
C NN + + T + + + G I ++V+G D+ AVY A K A+++ +
Sbjct: 255 LFCRNNGFAISTPSHEQYKGDGIAGRGPMGYGITTIRVDGTDVFAVYNAMKAAREYVLKE 314
Query: 289 NGPIVLEYETYRYGGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDEIK 348
N P+V E YR G HS SD T YR +EI+ S PI+ LK +++ G E
Sbjct: 315 NKPVVFEALAYRVGHHSTSDDSTAYRPAEEIEIWNSVEHPISKLKRYMVHKGWFDETVEN 374
Query: 349 AYDKAARKYVDEQVELADAAPAPEAKMSILFEDVY 383
Y K RK V +Q+ +++ P + +FE VY
Sbjct: 375 EYVKDIRKKVLKQIAVSEKKLKPNWRE--MFEGVY 407
>At1g21400 [C] KOG1182 Branched chain alpha-keto acid dehydrogenase complex
alpha subunit
Length = 472
Score = 136 bits (343), Expect = 5e-32
Identities = 91/334 (27%), Positives = 161/334 (47%), Gaps = 20/334 (5%)
Query: 61 VPELSYQTTKSNL-LQMYKDMIIVRRMEMACDALYKAKKIRGFCHSSVGQEAIAVGIENA 119
+P+ + L ++MY+ M ++ M+ + +I F +SVG+EAI + A
Sbjct: 117 IPDSDFIPVSEKLAVRMYEQMATLQVMDHIFYEAQRQGRI-SFYLTSVGEEAINIASAAA 175
Query: 120 ITKRDTVITSYRCHGFTYMRGAAVQAVLAELMGRRTGVSFGKGGSMHLYAPGFYGGNGI- 178
++ D V+ YR G RG ++ + G + +GKG M ++ YG N +
Sbjct: 176 LSPDDVVLPQYREPGVLLWRGFTLEEFANQCFGNKA--DYGKGRQMPIH----YGSNRLN 229
Query: 179 -------VGAQVPLGAGLAFAHQYKHEDACSFALYGDGASNQGQVFESFNMAKLWNLPAV 231
+ Q+P AG+ ++ + ++AC+ GDG +++G N A + P V
Sbjct: 230 YFTISSPIATQLPQAAGVGYSLKMDKKNACTVTFIGDGGTSEGDFHAGLNFAAVMEAPVV 289
Query: 232 FCCENNKYGMGTAAARSSAMTEYFKRGQY--IPGLKVNGMDILAVYQASKFAKDWTVSGN 289
F C NN + + T + +GQ I ++V+G D LAVY A + A++ V+
Sbjct: 290 FICRNNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVTEQ 349
Query: 290 GPIVLEYETYRYGGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDEIKA 349
P+++E TYR G HS SD T YR DEIQ+ + +P+ + + + G +E++
Sbjct: 350 RPVLIEMMTYRVGHHSTSDDSTKYRAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDESK 409
Query: 350 YDKAARKYVDEQVELADAAPAPEAKMSILFEDVY 383
ARK + + ++ A+ + ++ LF DVY
Sbjct: 410 LRSNARKQLLQAIQAAE--KWEKQPLTELFNDVY 441
>At5g09300 [C] KOG1182 Branched chain alpha-keto acid dehydrogenase complex
alpha subunit
Length = 414
Score = 129 bits (324), Expect = 8e-30
Identities = 88/334 (26%), Positives = 160/334 (47%), Gaps = 24/334 (7%)
Query: 67 QTTKSNLLQMYKDMIIVRRMEMACDALYKAKKIR--GFCHSSVGQEAIAVGIENAITKRD 124
Q ++ +++Y DM+ ++ M+ + Y+A++ F +++G+EAI + A+T +D
Sbjct: 66 QVSEEVAVKIYSDMVTLQIMD---NIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQD 122
Query: 125 TVITSYRCHGFTYMRGAAVQAVLAELMGRRTGVSFGKGGSMHLYAPGFYGGNGI------ 178
+ YR G RG +Q + G ++ +GKG M P YG N +
Sbjct: 123 VIFPQYREPGVLLWRGFTLQEFANQCFGNKS--DYGKGRQM----PVHYGSNKLNYFTVS 176
Query: 179 --VGAQVPLGAGLAFAHQYKHEDACSFALYGDGASNQGQVFESFNMAKLWNLPAVFCCEN 236
+ Q+P G A++ + +DAC+ +GDG +++G + N+A + P +F C N
Sbjct: 177 ATIATQLPNAVGAAYSLKMDKKDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRN 236
Query: 237 NKYGMGTAAARSSAMTEYFKRGQY--IPGLKVNGMDILAVYQASKFAKDWTVSGNGPIVL 294
N + + T + +G+ I ++V+G D LA+Y A A++ + PI++
Sbjct: 237 NGWAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILI 296
Query: 295 EYETYRYGGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDEIKAYDKAA 354
E TYR G HS SD T YR+ EI+ +P++ + + G ++
Sbjct: 297 EALTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRI 356
Query: 355 RKYVDEQVELADAAPAPEAKMSILFEDVY-VPGS 387
+K + E + +A+ P + +F DVY VP S
Sbjct: 357 KKEMLEALRVAEKTEKP--NLQNMFSDVYDVPPS 388
>CE16618 [C] KOG1182 Branched chain alpha-keto acid dehydrogenase complex
alpha subunit
Length = 478
Score = 114 bits (285), Expect = 3e-25
Identities = 78/224 (34%), Positives = 113/224 (49%), Gaps = 10/224 (4%)
Query: 182 QVPLGAGLAFAH-QYKHEDACSFALYGDGASNQGQVFESFNMAKLWNLPAVFCCENNKYG 240
Q+P G A+A Q K + + +GDGA+++G +FN A P +F C NN Y
Sbjct: 242 QLPQAVGSAYAFKQQKDNNRIAVVYFGDGAASEGDAHAAFNFAATLKCPIIFFCRNNGYA 301
Query: 241 MGTAAARSSAMTEYFKRGQY--IPGLKVNGMDILAVYQASKFAKDWTVSGNGPIVLEYET 298
+ T + +G + ++V+G D+LAVY A+K A+ ++ N P+++E T
Sbjct: 302 ISTPTSEQYGGDGIAGKGPAYGLHTIRVDGNDLLAVYNATKEARRVALT-NRPVLIEAMT 360
Query: 299 YRYGGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDEIKAYDKAARKYV 358
YR G HS SD T YR+ DE+Q K+ PI K ++ E G E++ + K +K V
Sbjct: 361 YRLGHHSTSDDSTAYRSSDEVQTWGDKDHPITRFKKYITERGWWNEEKEMEWQKEVKKRV 420
Query: 359 DEQVELADAAPAPEAKMSILFEDVYVPGSETPTLRGRLQEDTWD 402
E A A +A LFEDVY E P LR R Q D D
Sbjct: 421 --LTEFAAAEKRKKAHYHDLFEDVY---DELP-LRLRRQRDELD 458
>At5g34780_1 [C] KOG1182 Branched chain alpha-keto acid dehydrogenase complex
alpha subunit
Length = 288
Score = 95.9 bits (237), Expect = 1e-19
Identities = 58/198 (29%), Positives = 97/198 (48%), Gaps = 4/198 (2%)
Query: 188 GLAFAHQYKHEDACSFALYGDGASNQGQVFESFNMAKLWNLPAVFCCENNKYGMGTAAAR 247
GL+ A ++AC+ GDG +++G N A + P VF C NN + + T +
Sbjct: 16 GLSKAKDCWEKNACAVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICRNNGWAISTHISE 75
Query: 248 SSAMTEYFKRGQY--IPGLKVNGMDILAVYQASKFAKDWTVSGNGPIVLEYETYRYGGHS 305
+GQ I ++V+G D LAVY A A++ V+ P+++E YR G HS
Sbjct: 76 QFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVCSAREMAVTEQRPVLIEMMIYRVGHHS 135
Query: 306 MSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDEIKAYDKAARKYVDEQVELA 365
SD T YR DEIQ+ + + + + + + G +E++ ARK + + ++ A
Sbjct: 136 TSDDSTKYRAADEIQYWKMSRNSVNRFRKSVEDNGWWSEEDESKLRSNARKQLLQAIQAA 195
Query: 366 DAAPAPEAKMSILFEDVY 383
+ + ++ LF DVY
Sbjct: 196 E--KWEKQPLTELFNDVY 211
>At5g65750 [G] KOG0450 2-oxoglutarate dehydrogenase E1 subunit
Length = 1025
Score = 50.1 bits (118), Expect = 6e-06
Identities = 49/192 (25%), Positives = 84/192 (43%), Gaps = 14/192 (7%)
Query: 184 PLGAGLAFAHQYKHEDA-----CSFALYGDGA-SNQGQVFESFNMAKLWNL---PAVFCC 234
P+ G A QY +D ++GDG+ + QG V+E+ +++ L N V
Sbjct: 374 PVVIGKTRAKQYYTKDENRTKNMGILIHGDGSFAGQGVVYETLHLSALPNYCTGGTVHIV 433
Query: 235 ENNKYGMGT--AAARSSAMTEYFKRGQYIPGLKVNGMDILAVYQASKFAKDWTVSGNGPI 292
NN+ T RSS + P VN DI AV A + A +W + + +
Sbjct: 434 VNNQVAFTTDPREGRSSQYCTDVAKALSAPIFHVNADDIEAVVHACELAAEWRQTFHSDV 493
Query: 293 VLEYETYRYGGHS-MSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLLELGIATEDEIKAYD 351
V++ YR GH+ + +P T ++ +RS + + LL+ G T+++I
Sbjct: 494 VVDLVCYRRFGHNEIDEPSFTQPKMYKV--IRSHPSSLQIYQEKLLQSGQVTQEDIDKIQ 551
Query: 352 KAARKYVDEQVE 363
K ++E+ E
Sbjct: 552 KKVSSILNEEYE 563
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.134 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,467,865
Number of Sequences: 60738
Number of extensions: 1080217
Number of successful extensions: 2432
Number of sequences better than 1.0e-05: 18
Number of HSP's better than 0.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2389
Number of HSP's gapped (non-prelim): 20
length of query: 412
length of database: 30,389,216
effective HSP length: 109
effective length of query: 303
effective length of database: 23,768,774
effective search space: 7201938522
effective search space used: 7201938522
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)