ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI3155 good O KOG2903 Posttranslational modification, protein turnover, chaperones Predicted glutathione S-transferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI3155 1110886 1109804 -361
(361 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKR076w [O] KOG2903 Predicted glutathione S-transferase 490 e-138
YMR251w [O] KOG2903 Predicted glutathione S-transferase 414 e-115
SPCC1281.07c [O] KOG2903 Predicted glutathione S-transferase 343 2e-94
YGR154c [O] KOG2903 Predicted glutathione S-transferase 338 6e-93
At5g45020 [O] KOG2903 Predicted glutathione S-transferase 217 2e-56
At5g44990 [O] KOG2903 Predicted glutathione S-transferase 199 5e-51
At4g19880 [O] KOG2903 Predicted glutathione S-transferase 192 5e-49
At5g44000 [O] KOG2903 Predicted glutathione S-transferase 146 5e-35
>YKR076w [O] KOG2903 Predicted glutathione S-transferase
Length = 370
Score = 490 bits (1262), Expect = e-138
Identities = 241/370 (65%), Positives = 296/370 (79%), Gaps = 9/370 (2%)
Query: 1 MSKKFAS-EDGSFKRQVSSFRETISSTHPIYKPEKGRYWLYVSLACPWAHRTLITRVLKG 59
MSK++AS +G+FKRQVSSFRETIS HPIYKP KGRYWLYVSLACPWAHRTLITR LKG
Sbjct: 1 MSKQWASGTNGAFKRQVSSFRETISKQHPIYKPAKGRYWLYVSLACPWAHRTLITRALKG 60
Query: 60 LTSVIGISVVHWHMDEKGWRFLPYNES--NKTDGKESYKPSAG--LKCEKDLCVS-ADVD 114
LTSVIG SVVHWH+DEKGWRFL + + D E + AG ++D S A++
Sbjct: 61 LTSVIGCSVVHWHLDEKGWRFLDMEKQLEDSEDFLEHWHDVAGGIRTAKEDSSKSFAEIK 120
Query: 115 NHSIKYGIDGTIDHNYHFQRISELYFKSEPEYAARFTVPVLWDLKTQTIVNNESSEIIRI 174
N S ++ +D T + +Y ++RIS+LY+KS+P+Y+ARFTVPVLWDL+TQTIVNNESSEIIRI
Sbjct: 121 NDSQRFMVDATNEPHYGYKRISDLYYKSDPQYSARFTVPVLWDLETQTIVNNESSEIIRI 180
Query: 175 FNSGVFNE-VADSHPDIDLVPKELESQIDEINSWVYDSINNGVYKTGFAEKQDIYEKEVL 233
NS F+E V D H DLVP +L++QID+ NSWVYDSINNGVYKTGFAEK ++YE EV
Sbjct: 181 LNSSAFDEFVDDDHKKTDLVPAQLKTQIDDFNSWVYDSINNGVYKTGFAEKAEVYESEVN 240
Query: 234 NVFKHLDKVEGILKKRYETLKSTHSSEE--EIVKKLYLLGDQLTEADLRLYPTIVRFDPV 291
NVF+HLDKVE IL +Y LK+ + E+ +I+ + + +GDQLTEAD+RLY T++RFDPV
Sbjct: 241 NVFEHLDKVEKILSDKYSKLKAKYGEEDRQKILGEFFTVGDQLTEADIRLYTTVIRFDPV 300
Query: 292 YVQHFKTNLNTIRSGYPYIHLWLRNLYWNYEAFQETTNFNHIKWHYTRSHTKINPLSITP 351
YVQHFK N +IR+GYP+IHLW+RNLYWNY+AF+ TT+F+HIK HYTRSHT+INPL ITP
Sbjct: 301 YVQHFKCNFTSIRAGYPFIHLWVRNLYWNYDAFRYTTDFDHIKLHYTRSHTRINPLGITP 360
Query: 352 LGPSPNILPL 361
LGP P+I PL
Sbjct: 361 LGPKPDIRPL 370
>YMR251w [O] KOG2903 Predicted glutathione S-transferase
Length = 366
Score = 414 bits (1064), Expect = e-115
Identities = 203/367 (55%), Positives = 271/367 (73%), Gaps = 9/367 (2%)
Query: 1 MSKKFASED-GSFKRQVSSFRETISSTHPIYKPEKGRYWLYVSLACPWAHRTLITRVLKG 59
MS+K AS + FKRQ S FRE IS+ HPIYKP KGRYWLYV+L CPWA RTLITR LKG
Sbjct: 1 MSEKSASNNKAEFKRQSSPFREIISADHPIYKPAKGRYWLYVALPCPWAQRTLITRALKG 60
Query: 60 LTSVIGISVVHWHMDEKGWRFLPYNESNKTDGKESYKPSAGLKCEKDLCVS---ADVDNH 116
L +IG SV HWH+D+KGWRFL + KT+ + + + G+ +L S A++ N+
Sbjct: 61 LAPIIGCSVAHWHLDDKGWRFLEEGDG-KTNERHWFDIAGGIS-SVNLNTSTPVANIPNN 118
Query: 117 SIKYGIDGTIDHNYHFQRISELYFKSEPEYAARFTVPVLWDLKTQTIVNNESSEIIRIFN 176
+ + +DGT + +Y ++R+S+ YFK++P+Y RFTVPVLWDL+T TIVNNESS+II I N
Sbjct: 119 AHRLLVDGTDEPHYGYKRLSDFYFKTKPDYKGRFTVPVLWDLETCTIVNNESSDIIGIMN 178
Query: 177 SGVFNE-VADSHPDIDLVPKELESQIDEINSWVYDSINNGVYKTGFAEKQDIYEKEVLNV 235
S F+E V + + + LVP+ LE+QI E NSWVYD INNGVYK GFAE ++YE+EV ++
Sbjct: 179 SAAFDEFVGEEYRQVRLVPRSLEAQITEFNSWVYDKINNGVYKAGFAECAEVYEREVTSL 238
Query: 236 FKHLDKVEGILKKRYETLKSTH--SSEEEIVKKLYLLGDQLTEADLRLYPTIVRFDPVYV 293
F++LDK+E +L K+Y L++ + +++++I+ + + +GD LTEAD+RLYPTIVRFD VY
Sbjct: 239 FQYLDKLENLLDKKYTDLEAEYGKNNKDKILDRYFAIGDTLTEADVRLYPTIVRFDVVYH 298
Query: 294 QHFKTNLNTIRSGYPYIHLWLRNLYWNYEAFQETTNFNHIKWHYTRSHTKINPLSITPLG 353
QHFK NL TIR Y IH WL+N+YW +EAFQ TT+F HIK YTRS ++NP+ ITPLG
Sbjct: 299 QHFKCNLATIRDDYSRIHTWLKNIYWRHEAFQRTTDFTHIKLGYTRSQPRVNPIGITPLG 358
Query: 354 PSPNILP 360
P P+I P
Sbjct: 359 PKPDIRP 365
>SPCC1281.07c [O] KOG2903 Predicted glutathione S-transferase
Length = 313
Score = 343 bits (880), Expect = 2e-94
Identities = 182/356 (51%), Positives = 223/356 (62%), Gaps = 56/356 (15%)
Query: 5 FASEDGSFKRQVSSFRETISSTHPIYKPEKGRYWLYVSLACPWAHRTLITRVLKGLTSVI 64
++S+DG F+RQVSSFRE IS H ++PEK RY LYVS ACPWAHRTLI R LKGL +VI
Sbjct: 9 WSSKDGEFRRQVSSFRERISPEHKYFQPEKDRYHLYVSYACPWAHRTLIVRKLKGLENVI 68
Query: 65 GISVVHWHMDEKGWRFLPYNESNKTDGKESYKPSAGLKCEKDLCVSADVDNHSIKYGIDG 124
+ VV W M GW F D +N D
Sbjct: 69 PVHVVGWLMGPNGWNF-------------------------------DKEN-------DS 90
Query: 125 TIDHNYHFQRISELYFKSEPEYAARFTVPVLWDLKTQTIVNNESSEIIRIFNSGVFNEVA 184
T D Y+ + LYF+++P Y RFTVPVLWD K TIVNNES+EIIR+FN FNEV
Sbjct: 91 TGDPLYNSPYLRNLYFRADPNYNMRFTVPVLWDSKYNTIVNNESAEIIRMFNDA-FNEVI 149
Query: 185 DSHPD--IDLVPKELESQIDEINSWVYDSINNGVYKTGFAEKQDIYEKEVLNVFKHLDKV 242
+ +DL P L ++IDE+N + YD++NNGVYKTGFA + YEK V VF+ LD++
Sbjct: 150 EDEEKRVVDLYPSSLRTKIDELNDYFYDTVNNGVYKTGFATTAEAYEKNVRVVFQGLDRL 209
Query: 243 EGILKKRYETLKSTHSSEEEIVKKLYLLGDQLTEADLRLYPTIVRFDPVYVQHFKTNLNT 302
E +LK+ K +LLGD LTE D+RLY TIVRFDPVYVQHFK N+ T
Sbjct: 210 EQVLKES---------------KGPFLLGDHLTETDVRLYTTIVRFDPVYVQHFKCNIGT 254
Query: 303 IRSGYPYIHLWLRNLYWNYEAFQETTNFNHIKWHYTRSHTKINPLSITPLGPSPNI 358
IR YP+I+ WL+ LYW + AF ETT+F HIK HYT+SHT+INPL ITPLGP PN+
Sbjct: 255 IRHNYPHINQWLKRLYWKHPAFHETTDFKHIKCHYTQSHTQINPLGITPLGPIPNV 310
>YGR154c [O] KOG2903 Predicted glutathione S-transferase
Length = 356
Score = 338 bits (868), Expect = 6e-93
Identities = 173/350 (49%), Positives = 234/350 (66%), Gaps = 9/350 (2%)
Query: 18 SFRETISSTHPIYKPEKGRYWLYVSLACPWAHRTLITRVLKGLTSVIGISVVHWHMDEKG 77
S++ TIS TH ++KPEKGRY++Y +L CP+ HR ++ R LK L V+G+ + HW +D KG
Sbjct: 4 SYKGTISKTHSVFKPEKGRYYIYGALGCPFTHRAILARSLKKLEPVLGLVLSHWQLDSKG 63
Query: 78 WRFLPYNESNKTDGKESYKPSAGLKCEK--DLCVSADVDNHSIKYGIDGTIDHNYHFQRI 135
RFLP + + + + G+ K + DV+N S + +DG D + R+
Sbjct: 64 ARFLPAPHRPEKYKERFFTATGGIASAKLDESEELGDVNNDSARLFVDGAFDPVENISRL 123
Query: 136 SELYFKSEPEY-AARFTVPVLWDLKTQTIVNNESSEIIRIFNSGVFNEVADSHPD--IDL 192
SELY+ ++P+Y +FTVPVLWD KT+ IVNNES +IIRI NSGVF+E S IDL
Sbjct: 124 SELYYLNDPKYPGTKFTVPVLWDSKTRKIVNNESGDIIRILNSGVFDEFIQSEETNVIDL 183
Query: 193 VPKELESQIDEINSWVYDSINNGVYKTGFAEKQDIYEKEVLNVFKHLDKVEGILKKRYET 252
VP +L +ID+ WV+ IN GVYK G AE IYE EV +F++L K+E +LK+ Y+
Sbjct: 184 VPHDLIDEIDKNIKWVHPKINLGVYKVGLAENGKIYETEVKTLFENLQKMECVLKENYKR 243
Query: 253 LKSTHS-SEEEIVKKLYLLGDQLTEADLRLYPTIVRFDPVYVQHFKTNLNTIRSGYPYIH 311
L+ S ++++I+ K ++LG +LTEAD+RLYP+I+RFD VYVQHFK NL TIR G+PY+H
Sbjct: 244 LEEQFSGNKQKILAKYFVLGQRLTEADIRLYPSIIRFDVVYVQHFKCNLKTIRDGFPYLH 303
Query: 312 LWLRNLYWNYEAFQETTNFNHIKWHYTR---SHTKINPLSITPLGPSPNI 358
LWL NLYWNY F+ TT+FNHIK Y R S KIN I PLGP P+I
Sbjct: 304 LWLINLYWNYAEFRFTTDFNHIKLFYIRMEVSRNKINQFGIVPLGPKPDI 353
>At5g45020 [O] KOG2903 Predicted glutathione S-transferase
Length = 325
Score = 217 bits (552), Expect = 2e-56
Identities = 135/357 (37%), Positives = 182/357 (50%), Gaps = 63/357 (17%)
Query: 7 SEDGSFKRQVSSFRETISSTHPIYKP-EKGRYWLYVSLACPWAHRTLITRVLKGLTSVIG 65
SE G+F R S+FR +S P E GRY LY+S ACPWA R L +KGL I
Sbjct: 10 SESGAFVRTASTFRNFVSQDPDSQFPAESGRYHLYISYACPWACRCLSYLKIKGLDEAIT 69
Query: 66 ISVVH--WHM-----DEKGWRFLPYNESNKTDGKESYKPSAGLKCEKDLCVSADVDNHSI 118
S VH W D +GW F P +++ + Y A
Sbjct: 70 FSSVHAIWGRTKETDDHRGWVF-PDSDTELPGAEPDYLNGA------------------- 109
Query: 119 KYGIDGTIDHNYHFQRISELYFKSEPEYAARFTVPVLWDLKTQTIVNNESSEIIRIFNSG 178
+ + ELY + P Y ++TVPVLWD K +T+VNNESSEIIR+FN+
Sbjct: 110 --------------KSVRELYEIASPNYEGKYTVPVLWDKKLKTVVNNESSEIIRMFNTE 155
Query: 179 VFNEVADSHPDIDLVPKELESQIDEINSWVYDSINNGVYKTGFAEKQDIYEKEVLNVFKH 238
FN +A + P +DL P L I+E N WV++ INNGVYK GFA KQ+ Y + V +++
Sbjct: 156 -FNGIAKT-PSLDLYPSHLRDVINETNGWVFNGINNGVYKCGFARKQEPYNEAVNQLYEA 213
Query: 239 LDKVEGILKKRYETLKSTHSSEEEIVKKLYLLGDQLTEADLRLYPTIVRFDPVYVQHFKT 298
+D+ E +L K+ Y+ G+ TEAD+RL+ T++RFD VY HFK
Sbjct: 214 VDRCEEVLG-----------------KQRYICGNTFTEADIRLFVTLIRFDEVYAVHFKC 256
Query: 299 NLNTIRSGYPYIHLWLRNLYWNYEAFQETTNFNHIKWHYTRSHTKINPLSITPLGPS 355
N +R YP I +++++Y T N HIK HY SH INP I P GP+
Sbjct: 257 NKRLLRE-YPNIFNYIKDIY-QIHGMSSTVNMEHIKQHYYGSHPTINPFGIIPHGPN 311
>At5g44990 [O] KOG2903 Predicted glutathione S-transferase
Length = 350
Score = 199 bits (506), Expect = 5e-51
Identities = 129/356 (36%), Positives = 181/356 (50%), Gaps = 49/356 (13%)
Query: 1 MSKKFASEDGSFKRQVSSFRETISSTHPIYKP-EKGRYWLYVSLACPWAHRTLITRVLKG 59
M+ +E+ +F R +SFR +S P E GRY LY+S ACPWA R L LKG
Sbjct: 1 MATPMENENPNFARTATSFRNFVSKDPDSQFPAESGRYHLYISYACPWASRCLAILKLKG 60
Query: 60 LTSVIGISVVHWHMDEKGWRFLPYNESNKTDGKESYKPSAGLKCEKDLCVSADVDNHSIK 119
L I S V + WR N+ + V D D +
Sbjct: 61 LDKAISFSSV-----QPLWRNTKENDEHMG------------------WVFPDSDTEVL- 96
Query: 120 YGIDGTIDHNYHFQRISELYFKSEPEYAARFTVPVLWDLKTQTIVNNESSEIIRIFNSGV 179
DH + + ELY + Y ++TVPVLWD K +TIVNNESSEI+R+FN+
Sbjct: 97 ---GAERDHINGAKSVRELYDIASSNYTGKYTVPVLWDKKLKTIVNNESSEILRMFNTE- 152
Query: 180 FNEVADSHPDIDLVPKELESQIDEINSWVYDSINNGVYKTGFAEKQDIYEKEVLNVFKHL 239
FN VA++ P +DL P L + IDE N W++D INNGVYK GFA Q+ Y+ EV +++ L
Sbjct: 153 FNHVAEN-PSLDLYPPNLRAIIDETNEWIHDGINNGVYKCGFATNQETYDVEVKRLYEAL 211
Query: 240 DKVEGILKKRYETLKSTHSSEEEIVKKLYLLGDQLTEADLRLYPTIVRFDPVYVQHFKTN 299
D+ E IL+ K+ +L G+ LTE+D+RL+ T++RFD Y FK +
Sbjct: 212 DRCEDILR-----------------KQRFLCGNTLTESDIRLFVTVIRFDEAYAVIFKCD 254
Query: 300 LNTIRSGYPYIHLWLRNLYWNYEAFQETTNFNHIKWHYTRSHTKINPLSITPLGPS 355
+R Y ++ + +++Y T +HIK +Y S INPL I GP+
Sbjct: 255 KRLVRE-YYHLFNYTKDIY-QIAGMSSTVKMDHIKQNYYGSFPSINPLEIIAHGPN 308
>At4g19880 [O] KOG2903 Predicted glutathione S-transferase
Length = 239
Score = 192 bits (489), Expect = 5e-49
Identities = 102/229 (44%), Positives = 139/229 (60%), Gaps = 21/229 (9%)
Query: 127 DHNYHFQRISELYFKSEPEYAARFTVPVLWDLKTQTIVNNESSEIIRIFNSGVFNEVADS 186
DH + + ELY + P Y ++TVPVLWD K +T+VNNES+EIIR+FN+ FN +A
Sbjct: 18 DHLNGAKSVRELYEIASPNYTGKYTVPVLWDKKLKTVVNNESAEIIRMFNTE-FNHIA-G 75
Query: 187 HPDIDLVPKELESQIDEINSWVYDSINNGVYKTGFAEKQDIYEKEVLNVFKHLDKVEGIL 246
+PD+DL P L+++IDE N W+Y+ INNGVY+ GFA+KQ YE+ V V++ LD+ E IL
Sbjct: 76 NPDLDLYPSHLQAKIDETNEWIYNGINNGVYRCGFAKKQGPYEEAVEQVYEALDRCEEIL 135
Query: 247 KKRYETLKSTHSSEEEIVKKLYLLGDQLTEADLRLYPTIVRFDPVYVQHFKTNLNTIRSG 306
K Y+ G+ LTE D+RL+ T++RFD VY HFK N IR
Sbjct: 136 GKH-----------------RYICGNTLTETDIRLFVTLIRFDEVYAVHFKCNKKLIRE- 177
Query: 307 YPYIHLWLRNLYWNYEAFQETTNFNHIKWHYTRSHTKINPLSITPLGPS 355
YP + + ++++ T N NHIK HY SH INP I P GP+
Sbjct: 178 YPNLFNYTKDIF-QIPGMSSTVNMNHIKQHYYGSHPSINPFGIIPHGPN 225
>At5g44000 [O] KOG2903 Predicted glutathione S-transferase
Length = 377
Score = 146 bits (368), Expect = 5e-35
Identities = 108/346 (31%), Positives = 158/346 (45%), Gaps = 54/346 (15%)
Query: 6 ASEDGSFKRQVSSFRETISSTHPIYKPEKGRYWLYVSLACPWAHRTLITRVLKGLTSVIG 65
+ GS+ R S FR + T LYV L CPWAHRTLI R LKGL +
Sbjct: 56 SDSSGSYTRPTSKFR--LDPTQ-FTSAASSELHLYVGLPCPWAHRTLIVRALKGLNDAVP 112
Query: 66 ISVVHWHMDEKGWRFLPYNESNKTDGKESYKPSAGLKCEKDLCVSADVDNHSIKYGIDGT 125
+S+ D W F N + K+ PS
Sbjct: 113 VSIASPGQDGS-WEFK--NNNIPIKDKDKLIPS--------------------------- 142
Query: 126 IDHNYHFQRISELYFKSEPEYAARFTVPVLWDLKTQTIVNNESSEIIRIFNSGVFNEVAD 185
+D + + E+Y Y R TVP+LWDL+ + +V NES +II FNSG+ N++A
Sbjct: 143 LDKANRCRNLKEVYKSRSGGYDGRCTVPMLWDLRKKDVVCNESYDIIEFFNSGL-NKLA- 200
Query: 186 SHPDIDLVPKELESQIDEINSWVYDSINNGVYKTGFAEKQDIYEKEVLNVFKHLDKVEGI 245
+ ++DL P EL+ I N VY +NNGVY+ GFA+ Q+ Y+ V +F LD++
Sbjct: 201 RNDNLDLSPPELKEMIQGWNQIVYPKVNNGVYRCGFAQSQEAYDGAVNELFSTLDEI--- 257
Query: 246 LKKRYETLKSTHSSEEEIVKKLYLLGDQLTEADLRLYPTIVRFDPVYVQHFKTNLNTIRS 305
E+ + YL G++LT AD+ L+ T++RFD VY FK +
Sbjct: 258 --------------EDHLGSNRYLCGERLTLADVCLFTTLIRFDSVYNILFKCTKKKLVE 303
Query: 306 GYPYIHLWLRNLYWNYEAFQETTNFNHIKWHYTRSHTKINPLSITP 351
YP ++ +LR +Y T + + I Y ++ +N I P
Sbjct: 304 -YPNLYGYLREIY-QIPGVAATCDISAIMDGYYKTLFPLNASGIQP 347
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.136 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,251,683
Number of Sequences: 60738
Number of extensions: 1036359
Number of successful extensions: 3152
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3115
Number of HSP's gapped (non-prelim): 12
length of query: 361
length of database: 30,389,216
effective HSP length: 108
effective length of query: 253
effective length of database: 23,829,512
effective search space: 6028866536
effective search space used: 6028866536
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)