ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI3242 good E KOG2663 Amino acid transport and metabolism Acetolactate synthase, small subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI3242 1139357 1140238 294
(294 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YCL009c [E] KOG2663 Acetolactate synthase small subunit 427 e-120
SPBC14C8.04 [E] KOG2663 Acetolactate synthase small subunit 226 4e-59
At5g16290 [E] KOG2663 Acetolactate synthase small subunit 87 3e-17
At2g31810 [E] KOG2663 Acetolactate synthase small subunit 72 7e-13
>YCL009c [E] KOG2663 Acetolactate synthase small subunit
Length = 309
Score = 427 bits (1099), Expect = e-120
Identities = 215/298 (72%), Positives = 249/298 (83%), Gaps = 8/298 (2%)
Query: 5 RVVPLALRSFARAKSSSTSALAYKQLHRNKARPPLPTIETPSWSTNSAISSILYENPVIS 64
RVV + + R SSSTSALAYKQ+HR+ RPPLPT++TPSW+ NSA+SSI+YE P S
Sbjct: 12 RVVASSCATMVRCSSSSTSALAYKQMHRHATRPPLPTLDTPSWNANSAVSSIIYETPAPS 71
Query: 65 KEPKKQHVLNCLVQNEPGVLSLVSGTLAARGFNIDSLVVCNTEVKDLSRMTIVLQGQDGV 124
++P+KQHVLNCLVQNEPGVLS VSGTLAARGFNIDSLVVCNTEVKDLSRMTIVLQGQDGV
Sbjct: 72 RQPRKQHVLNCLVQNEPGVLSRVSGTLAARGFNIDSLVVCNTEVKDLSRMTIVLQGQDGV 131
Query: 125 IEQARRQIEDLVPVFVVLDYSHSTIIERELLMTRISLLGAEYFEDLIHHHEQDENT---- 180
+EQARRQIEDLVPV+ VLDY++S II+REL+M RISLLG EYFEDL+ HH N
Sbjct: 132 VEQARRQIEDLVPVYAVLDYTNSEIIKRELVMARISLLGTEYFEDLLLHHHTSTNAGAAD 191
Query: 181 --DTVERIRQQQFHPSNLPLSQVLRLKHEHLNDISNLTGNFGGKIVDIAEQSCIVELCAK 238
+ V IR++QFHP+NLP S+VLRLKHEHLNDI+NLT NFGG++VDI+E SCIVEL AK
Sbjct: 192 SQELVAEIREKQFHPANLPASEVLRLKHEHLNDITNLTNNFGGRVVDISETSCIVELSAK 251
Query: 239 PSRISAFLKLVEPFGILEVARSGMMALPRTHLSVGDED--DAQSKMNDIVDISQLPPG 294
P+RISAFLKLVEPFG+LE ARSGMMALPRT L E+ D K+++IVDISQLPPG
Sbjct: 252 PTRISAFLKLVEPFGVLECARSGMMALPRTPLKTSTEEAADEDEKISEIVDISQLPPG 309
>SPBC14C8.04 [E] KOG2663 Acetolactate synthase small subunit
Length = 289
Score = 226 bits (575), Expect = 4e-59
Identities = 126/284 (44%), Positives = 180/284 (63%), Gaps = 10/284 (3%)
Query: 14 FARAKSSS-TSALAYKQLHRNKARPPLPTIETPSWSTNSAISSILYENPVISKEPKKQHV 72
F R KS+S TS + YK LH N P IE P + A+S+I+ P + K+HV
Sbjct: 13 FVRLKSTSATSPITYKALHANSPLPRCRIIEPPRATVPEAVSNIIMSTPFNRVQRPKRHV 72
Query: 73 LNCLVQNEPGVLSLVSGTLAARGFNIDSLVVCNTEVKDLSRMTIVLQGQDGVIEQARRQI 132
NCLVQNEPGVLS +SG LAARGFNIDSLVVC TEV++LSRMTIVL+G D V+EQA+RQI
Sbjct: 73 FNCLVQNEPGVLSRLSGILAARGFNIDSLVVCATEVENLSRMTIVLRGADEVVEQAKRQI 132
Query: 133 EDLVPVFVVLDYSHSTIIERELLMTRISLLGAEYFEDLIHHHEQDENTDTVERIRQQQFH 192
ED+V V+ VLDY+ ++++ERELL+ ++SLLG ++F++ H E+ E + +
Sbjct: 133 EDIVSVWAVLDYTGTSMVERELLLAKVSLLGPDHFQE---HFERSEKVAESTNAKAKSDG 189
Query: 193 PSNLPLSQVLRLKHEHLNDISNLTGNFGGKIVDIAEQSCIVELCAKPSRISAFLKLVEPF 252
+ + L+L+ L I+ LT F G++ DI+ ++ I+EL A P R+ FL L+ P+
Sbjct: 190 EGVMNANAALQLRASQLAAINQLTTLFHGRVADISTETIILELTATPDRVDNFLSLLRPY 249
Query: 253 GILEVARSGMMALPRTHLSVGDEDDAQSKMNDIVDISQ--LPPG 294
G+LE R+G A+ R S ++ + D V++ + LPPG
Sbjct: 250 GVLEACRTGTSAMTRAPHS----NEVTEEAEDDVEVEEVFLPPG 289
>At5g16290 [E] KOG2663 Acetolactate synthase small subunit
Length = 477
Score = 87.0 bits (214), Expect = 3e-17
Identities = 56/199 (28%), Positives = 97/199 (48%), Gaps = 43/199 (21%)
Query: 69 KQHVLNCLVQNEPGVLSLVSGTLAARGFNIDSLVVCNTEVKDLSRMTIVLQGQDGVIEQA 128
+ H L+ LV N PGVL+L++G ++ RG+NI SL V E + LSR+T V+ G D I++
Sbjct: 306 RSHTLSLLVANVPGVLNLITGAISRRGYNIQSLAVGPAEKEGLSRITTVIPGTDENIDKL 365
Query: 129 RRQIEDLVPVFVVLDYSHSTIIERELLMTRISLLGAEYFEDLIHHHEQDENTDTVERIRQ 188
RQ++ L+ + + + +H EREL++ +++ DT R
Sbjct: 366 VRQLQKLIDLQEIQNITHMPFAERELMLIKVA-------------------ADTSAR--- 403
Query: 189 QQFHPSNLPLSQVLRLKHEHLNDISNLTGNFGGKIVDIAEQSCIVELCAKPSRISAFLKL 248
D+ ++ F K +D+++ + +E+ ++SA
Sbjct: 404 ---------------------RDVLDIAQVFRAKAIDVSDHTITLEVTGDLRKMSALQTQ 442
Query: 249 VEPFGILEVARSGMMALPR 267
+E +GI EVAR+G +AL R
Sbjct: 443 LEAYGICEVARTGRVALVR 461
Score = 83.6 bits (205), Expect = 3e-16
Identities = 66/220 (30%), Positives = 101/220 (45%), Gaps = 46/220 (20%)
Query: 48 STNSAISSILYENPVISKEPKKQHVLNCLVQNEPGVLSLVSGTLAARGFNIDSLVVCNTE 107
ST+ AI++ P ++ H ++ V +E G+++ ++G A RG+NI+SL V E
Sbjct: 55 STDKAITTAQSVAPTACDRVRR-HTISVFVGDESGIINRIAGVFARRGYNIESLAVGLNE 113
Query: 108 VKDLSRMTIVLQGQDGVIEQARRQIEDLVPVFVVLDYSHSTIIERELLMTRISLLGAEYF 167
K L TIV+ G D V++Q Q+ LV V V D S +EREL++ ++
Sbjct: 114 DKAL--FTIVVLGTDKVLQQVVEQLNKLVNVIKVEDLSKEPHVERELMLIKL-------- 163
Query: 168 EDLIHHHEQDENTDTVERIRQQQFHPSNLPLSQVLRLKHEHLNDISNLTGNFGGKIVDIA 227
N D R ++I L F KIVD +
Sbjct: 164 -----------NADPSTR------------------------SEIMWLVDIFRAKIVDTS 188
Query: 228 EQSCIVELCAKPSRISAFLKLVEPFGILEVARSGMMALPR 267
EQS +E+ P ++ A +E FGI E+AR+G +AL R
Sbjct: 189 EQSLTIEVTGDPGKMVALTTNLEKFGIKEIARTGKIALRR 228
>At2g31810 [E] KOG2663 Acetolactate synthase small subunit
Length = 484
Score = 72.4 bits (176), Expect = 7e-13
Identities = 53/199 (26%), Positives = 90/199 (44%), Gaps = 53/199 (26%)
Query: 69 KQHVLNCLVQNEPGVLSLVSGTLAARGFNIDSLVVCNTEVKDLSRMTIVLQGQDGVIEQA 128
+ H L+ LV + PGVL++V+G+LA V + E K +SR+T V+ D + +
Sbjct: 321 RSHTLSLLVNDIPGVLNIVTGSLA----------VGHAETKGISRITTVIPATDESVSKL 370
Query: 129 RRQIEDLVPVFVVLDYSHSTIIERELLMTRISLLGAEYFEDLIHHHEQDENTDTVERIRQ 188
+Q+ LV V V D +H EREL++ +I++ A
Sbjct: 371 VQQLYKLVDVHEVHDLTHLPFSERELMLIKIAVNAAA----------------------- 407
Query: 189 QQFHPSNLPLSQVLRLKHEHLNDISNLTGNFGGKIVDIAEQSCIVELCAKPSRISAFLKL 248
D+ ++ F K VD+++ + ++L ++ A +L
Sbjct: 408 --------------------RRDVLDIASIFRAKAVDVSDHTITLQLTGDLDKMVALQRL 447
Query: 249 VEPFGILEVARSGMMALPR 267
+EP+GI EVAR+G +AL R
Sbjct: 448 LEPYGICEVARTGRVALAR 466
Score = 70.5 bits (171), Expect = 3e-12
Identities = 51/197 (25%), Positives = 90/197 (44%), Gaps = 45/197 (22%)
Query: 65 KEPKKQHVLNCLVQNEPGVLSLVSGTLAARGFNIDSLVVCNTEVKDLSRMTIVLQGQDGV 124
K ++H ++ V +E G+++ ++G A RG+NI+SL V +D + TIV+ G + V
Sbjct: 81 KSKVRKHTISVFVGDESGMINRIAGVFARRGYNIESLAVGLN--RDKALFTIVVCGTERV 138
Query: 125 IEQARRQIEDLVPVFVVLDYSHSTIIERELLMTRISLLGAEYFEDLIHHHEQDENTDTVE 184
++Q Q++ LV V V D S +EREL++ +++
Sbjct: 139 LQQVIEQLQKLVNVLKVEDISSEPQVERELMLVKVNA----------------------- 175
Query: 185 RIRQQQFHPSNLPLSQVLRLKHEHLNDISNLTGNFGGKIVDIAEQSCIVELCAKPSRISA 244
HP E +I L F ++VDIAE + +E+ P ++ A
Sbjct: 176 -------HP-------------ESRAEIMWLVDTFRARVVDIAEHALTIEVTGDPGKMIA 215
Query: 245 FLKLVEPFGILEVARSG 261
+ ++ F I E+ R+G
Sbjct: 216 VERNLKKFQIREIVRTG 232
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.134 0.376
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,144,234
Number of Sequences: 60738
Number of extensions: 700674
Number of successful extensions: 2204
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2191
Number of HSP's gapped (non-prelim): 10
length of query: 294
length of database: 30,389,216
effective HSP length: 106
effective length of query: 188
effective length of database: 23,950,988
effective search space: 4502785744
effective search space used: 4502785744
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)