ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI3345 good S KOG2490 Function unknown Predicted membrane protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI3345 1173806 1172235 -524
(524 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YER140w [S] KOG2490 Predicted membrane protein 340 4e-93
SPBC13G1.05 [S] KOG2490 Predicted membrane protein 190 5e-48
CE19819 [S] KOG2490 Predicted membrane protein 174 3e-43
7300336 [S] KOG2490 Predicted membrane protein 160 4e-39
At1g67960 [S] KOG2490 Predicted membrane protein 98 3e-20
>YER140w [S] KOG2490 Predicted membrane protein
Length = 556
Score = 340 bits (871), Expect = 4e-93
Identities = 191/500 (38%), Positives = 305/500 (60%), Gaps = 44/500 (8%)
Query: 64 QSEKSVEYDLEQIANILRVPFQVEQFFVFAILTCLNCFLYYFTIIPLRVF---------- 113
+ E +Y+LEQ+ N++R+P +E+F +FA+LT L+CFLYYFT++P+R+
Sbjct: 62 EEEHEDDYELEQLLNMIRIPMFLEKFMLFALLTSLDCFLYYFTVLPIRLIKGYVKQFKSY 121
Query: 114 --HVLVVKRDGPK-----------RIRQELLTTAMILGPCSILNVLDTSRIYHKIKGQNA 160
H + +R G K R +E +I+ +L+ LDTS++YH+IK Q+
Sbjct: 122 RQHYRLQQRSGHKNKIPFRYRITSREYKERCMIFIIVISSILLSKLDTSKLYHRIKRQST 181
Query: 161 IKLYMIFQVLEMTEKLLSSVGLDLYSIIMLKKSAK----HKTEMVMLYIACCLCLSFHAL 216
+KLYM+F VLEM +K+L+S+G L ++++ +K+++ HK +V + + ++ H
Sbjct: 182 MKLYMLFSVLEMADKMLASLGQSLLTVMLSRKNSERILLHKCLLVSMSLTY---VTIHGY 238
Query: 217 IYIYQILAMNVAVNSYSNSLWTLLLSMQFSELKSAVFKRIDKEGLFQLTMADVVERFQLI 276
+ +YQ +++N+AVNSYSN+L TLLLSMQF+E+KS+V K+ DKEG FQ+T+ADVVERF+L
Sbjct: 239 VLVYQAISLNIAVNSYSNALLTLLLSMQFAEIKSSVLKKFDKEGFFQITIADVVERFKLT 298
Query: 277 VFLTVIAVRNFIVARKSLRDVLPHSWNVHSTQSLIVGIFIGPIVTVIGSELIVDWIKHAY 336
+ L++ +RN SL + + W+ ST S+++ I GP+V+V+GSE++VDW KHAY
Sbjct: 299 LLLSITGLRNLQSWSSSLSNTSINFWSPRSTLSIVINILCGPMVSVVGSEVLVDWAKHAY 358
Query: 337 IIKFNRIRAHIYERYLQILSKDNLSNSIQFQKRLGLPFPPLIVAFLVLVWPAIRQTIKNN 396
I KFNRIR IY+++ I+ KD + + + + RLGLP P +V F+V+V P + ++ + +
Sbjct: 359 ITKFNRIRPQIYDKFYYIIYKDYSTRTHKLEDRLGLPLPAFVVLFIVMVRPTLFKSSEPS 418
Query: 397 IIWSTVLIIF----GFLWXXXXXXXXXXXXXRWTLHIQNK---QA--SLHPDPLYINGHL 447
+ S I+F FL +W+ I+ + QA ++ + Y+ G L
Sbjct: 419 YLPSLFRILFMGASVFLLALLAKFTLDLILIKWSKRIEQRFRDQAFNTVVTEEEYVPGLL 478
Query: 448 SAGRGMMDEDARKMTHS---SQTRTYSPTITPPGSPKLAAFEKKASIPPSLNEQRTKRDL 504
S G G +D R HS + R + +++P K + PPSLN+ R ++D
Sbjct: 479 SGGMGKVDVSTRIALHSDYNKENRIETESVSPMRKRKTTLTAE--CTPPSLNDIRRQKDS 536
Query: 505 KHPKSLESVERYKMVSKRIW 524
K+P+SLE+V RYKMVSKRIW
Sbjct: 537 KNPRSLENVARYKMVSKRIW 556
>SPBC13G1.05 [S] KOG2490 Predicted membrane protein
Length = 649
Score = 190 bits (482), Expect = 5e-48
Identities = 118/368 (32%), Positives = 190/368 (51%), Gaps = 63/368 (17%)
Query: 74 EQIANILRVPFQVEQFFVFAILTCLNCFLYYFTIIPLRV---------------FHVLVV 118
+ + N L P+ +E+ F+F ++ FLY FT+ P+RV F
Sbjct: 150 KHVENFLATPYAIEKTFLFGWFVSVDSFLYIFTLFPIRVLISFFTLSRCIFQGLFSTFFH 209
Query: 119 KRDGPKRI-----RQELLTTAMILGPCSILNVLDTSRIYHKIKGQNAIKLYMIFQVLEMT 173
+ P R + +LL +I ++ +D SR+YH I+ Q +I+ Y+++ VLE+
Sbjct: 210 RNSSPNRSLPRSRKIDLLKLLLIFSTSILIRKIDVSRLYHIIRAQASIRFYVLYNVLEIA 269
Query: 174 EKLLSSVGLD----LYSIIMLKKSAKHKTEMVMLYIACCLCLSF---HALIYIYQILAMN 226
++L ++G D L+S +L + + + + + L++ H L+ +YQI+ +N
Sbjct: 270 DRLCCALGQDVLDCLFSNHILSFNFWNPAGWMTFFYYFAISLAYMVLHTLVLLYQIITLN 329
Query: 227 VAVNSYSNSLWTLLLSMQFSELKSAVFKRIDKEGLFQLTMADVVERFQLIVFLTVIAVRN 286
V VNSYSN++ LL+S Q E+K AVFK+ +KE LFQLT +DVVERFQ+ + + +I +RN
Sbjct: 330 VTVNSYSNAVLALLMSNQLVEIKGAVFKKFEKENLFQLTCSDVVERFQITIMVIIIFLRN 389
Query: 287 FIVARKSLRDVLPHSWNVHSTQSL--------IVGIFIGPIVTVIGSELIVDWIKHAYII 338
+++T SL + + P VIGSEL VDW+KHA+II
Sbjct: 390 LA--------------ELYTTSSLDQPLLTFKRLKTLLAPFFWVIGSELFVDWLKHAFII 435
Query: 339 KFNRIRAHIYERYLQILSKDNLSN-----------SIQFQKRLGLPFPPLIVAFL---VL 384
KFN I+ IY R+ +L D +++ S Q +R+GLP PL+ F+ +
Sbjct: 436 KFNYIKPSIYSRFTDVLCHDYVASGAQLTQTVTGCSQQVARRMGLPVLPLVCVFIRTSMQ 495
Query: 385 VWPAIRQT 392
W R T
Sbjct: 496 TWSMFRST 503
>CE19819 [S] KOG2490 Predicted membrane protein
Length = 712
Score = 174 bits (441), Expect = 3e-43
Identities = 105/351 (29%), Positives = 185/351 (51%), Gaps = 42/351 (11%)
Query: 80 LRVPFQVEQFFVFAILTCLNCFLYYFTIIPLR----VFHVLVVKRDGPKRIRQELLTTAM 135
LR+P ++EQF + +L C++ F Y FT +PLR +F L+ + + L +
Sbjct: 175 LRIPLELEQFLTYGLLQCIDAFFYLFTFLPLRFLMSIFGALLRIKRWTSAETCDFLKVVI 234
Query: 136 ILGPCSILNVLDTSRIYHKIKGQNAIKLYMIFQVLEMTEKLLSSVGLDLYSIIMLKKSAK 195
I+ ++ +D+S +YH+++ Q IKLY+ + +LE+ ++L SS+G D++ ++ +++
Sbjct: 235 IVAASMLIREIDSSFLYHQVRSQGVIKLYIFYNMLEVADRLFSSLGQDIFDALLWTANSE 294
Query: 196 HKTEMVMLYIACC-------LCLSFHALIYIYQILAMNVAVNSYSNSLWTLLLSMQFSEL 248
K V +I C L + H+ + I Q +NVA NS++ ++ +++S F EL
Sbjct: 295 -KRFSVGYFIRTCGHLIVAILYATLHSFLVILQATTLNVAFNSHNQTVLAIMMSNNFVEL 353
Query: 249 KSAVFKRIDKEGLFQLTMADVVERFQLIVFLTVIAVRNFIVARKSLRDVLPHSWNVHSTQ 308
K +VFK+ K LFQ+ +DV ERF + L V+ +RN +WN+ S
Sbjct: 354 KGSVFKKFAKANLFQMACSDVRERFHIFALLFVVMIRNMTAV----------NWNIDSFT 403
Query: 309 SLIVGIFIGPIVTVIGSELIVDWIKHAYIIKFNRIRAHIYERYLQILSKDNLSN------ 362
+ I I+ V+G E VDW+KHA+I KFN I A +Y+ + ++ D + +
Sbjct: 404 EM-----IPDIIMVVGCEYFVDWLKHAFITKFNEINAEVYKDFTITIAFDVIRSRDQSAF 458
Query: 363 ---SIQFQKRLGLPFPPLIVAFLVLVWPAIRQTIKNNIIWSTVLIIFGFLW 410
S Q +R+G F P+ ++ +++ + T+ N W + IIFG W
Sbjct: 459 SDYSDQVSRRMG--FIPIPLSIMIIRVLSQTFTLDN---WGS-CIIFGIGW 503
>7300336 [S] KOG2490 Predicted membrane protein
Length = 593
Score = 160 bits (405), Expect = 4e-39
Identities = 116/369 (31%), Positives = 196/369 (52%), Gaps = 55/369 (14%)
Query: 74 EQIANILRVPFQVEQFFVFAILTCLNCFLYYFTIIPLRVFHVL--VVKRDGPKRIR---- 127
++I + +R+P +E+F V+ I+ C + FLY T +P+R + +V R + R
Sbjct: 19 QKIYSFMRIPRDLERFMVYGIMQCADSFLYIHTFLPVRFVMAVWALVSRTVARIFRLRSS 78
Query: 128 -QELLTTAMILGPCSILN------------VLDTSRIYHKIKGQNAIKLYMIFQVLEMTE 174
Q LL+ A I C +L ++DT+R+YH IK Q+ IKLY+ + +LE+ +
Sbjct: 79 GQRLLSPAEI---CDLLKGVIWMTVTLIMLLVDTNRVYHIIKSQSIIKLYIFYNMLEVGD 135
Query: 175 KLLSSVGLD----LYSIIMLKKSAKHKTEMVMLYIACCLCLSF-HALIYIYQILAMNVAV 229
+LLS+ G D L+ K++K + V+ ++ L F H+ + ++Q +NVAV
Sbjct: 136 RLLSAFGQDTIDALFWTATEPKNSKREHFGVLTHVLFTLIYVFLHSGLIMFQATCLNVAV 195
Query: 230 NSYSNSLWTLLLSMQFSELKSAVFKRIDKEGLFQLTMADVVERFQLIVFLTVIAVRNFIV 289
NS + L T+++S F ELK +VFK+ DK LFQLT +DV ERF L V L FIV
Sbjct: 196 NSNNKGLLTIMISNNFVELKGSVFKKFDKNNLFQLTCSDVRERFHLSVLL-------FIV 248
Query: 290 ARKSLRDVLPHSWNVHSTQSLIVGIFIGPIVTVIGSELIVDWIKHAYIIKFNRIRAHIYE 349
+++++ W++ TQ ++ + V+ +E+++DW+KHA+I +FN + IY
Sbjct: 249 VIQTMKE---FDWSI--TQFCVM---LPDCFAVLFTEILIDWVKHAFITRFNELPESIYR 300
Query: 350 RYLQILSKDNL---------SNSIQFQKRLG-LPFPPLIVAFLVLVWPAIRQTIKNNIIW 399
Y L+ D +S +R+G +PF PL V + ++ A+ + +N W
Sbjct: 301 EYTTSLAYDMTQTRQKHAFSDHSDLVARRMGFIPF-PLAVVLIKAIYTAV--SFENLAAW 357
Query: 400 STVLIIFGF 408
L+ + F
Sbjct: 358 LLFLLAYLF 366
>At1g67960 [S] KOG2490 Predicted membrane protein
Length = 587
Score = 98.2 bits (243), Expect = 3e-20
Identities = 83/323 (25%), Positives = 137/323 (41%), Gaps = 82/323 (25%)
Query: 71 YDLEQIANILRVPFQVEQ--FFVFAILTCLNCFLYYFTIIPLRVFHVLVVKRDGPKRI-- 126
+D I + R+ Q+ FFV C+N FL T++P+RV + + K +
Sbjct: 271 FDRMNICCLARILMQLIDTGFFV-----CVNSFLSLLTVMPIRVLLIFMDAFKNRKYLNN 325
Query: 127 ---RQELLTTAMILGP--CSILNVLDTSRIYHKIKGQNAIKLYMIFQVLEMTEKLLSSVG 181
RQ +A L C ++ D S IYH I+GQ+ IKLY+++
Sbjct: 326 ICGRQFRRPSASELSDLACFLVLATDISLIYHMIRGQSTIKLYVVYN------------- 372
Query: 182 LDLYSIIMLKKSAKHKTEMVMLYIACCLCLSFHALIYIYQILAMNVAVNSYSNSLWTLLL 241
I + + ++ + +++N+L LL+
Sbjct: 373 -------------------------------------ILEAITLSTCIVAHNNALLALLV 395
Query: 242 SMQFSELKSAVFKRIDKEGLFQLTMADVVERFQLIVFLTVIAVRNFIVARKSLRDVLPHS 301
S F+E+KS+VFKR K+ + L AD +ERF + FL + +N +
Sbjct: 396 SNNFAEIKSSVFKRFSKDNIHGLVYADSIERFHISAFLVSVLAQNIL------------- 442
Query: 302 WNVHSTQSLIVGIFIGPIVTVIGSELIVDWIKHAYIIKFNRIRAHIYERYLQILSKDNLS 361
++ G FI TV E+++D IKH+++ KFN I+ Y +LQ L + L
Sbjct: 443 ----ESEGAWFGNFIYNATTVFFCEMMIDIIKHSFLAKFNDIKPIAYSEFLQALCEQTL- 497
Query: 362 NSIQFQKRLGLPFPPLIVAFLVL 384
N ++ L F PL A +V+
Sbjct: 498 NIRPEDRKTNLTFVPLAPACVVI 520
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.325 0.138 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,258,091
Number of Sequences: 60738
Number of extensions: 1073945
Number of successful extensions: 3822
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3798
Number of HSP's gapped (non-prelim): 8
length of query: 524
length of database: 30,389,216
effective HSP length: 111
effective length of query: 413
effective length of database: 23,647,298
effective search space: 9766334074
effective search space used: 9766334074
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)