ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactVI3397   suspect: LH   S     KOG4534  Function unknown                                               Uncharacterized conserved protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI3397 1191512 1190223 -430 
         (430 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YLL002w [S] KOG4534 Uncharacterized conserved protein                 324   1e-88
SPBC342.06c [S] KOG4534 Uncharacterized conserved protein              67   5e-11

>YLL002w [S] KOG4534 Uncharacterized conserved protein
          Length = 436

 Score =  324 bits (831), Expect = 1e-88
 Identities = 181/431 (41%), Positives = 261/431 (59%), Gaps = 17/431 (3%)

Query: 1   MSLECYIKDALPFGHEFEIINLQSIPRETSPITFPKREAEDVNGKTVKTIKTQHFVALCF 60
           MSL  ++   LP   +FE ++LQSIP ET  +  P ++ + V      TIKTQHF +L  
Sbjct: 1   MSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRV---PKSTIKTQHFFSLFH 57

Query: 61  EEKVVFALEIVVYFSLNSANNGPVERLLFVSKADTNGYCDLRLDIGQITKSIIAYILAIS 120
           + KV F+LE+ VY +L   +    ERL+FVSKADTNGYC+ R+ +  ITK I+ +IL+I 
Sbjct: 58  QGKVFFSLEVYVYVTL--WDEADAERLIFVSKADTNGYCNTRVSVRDITKIILEFILSID 115

Query: 121 PGHYLRAVKPLIR--KALSGAKIIKRSTSVQRALEILSNRPLDSKG-ITSRVKIP--DQD 175
           P +YL+ VKP IR  K +S  ++I  +++  R L IL+ R   S   +   ++ P   QD
Sbjct: 116 PNYYLQKVKPAIRSYKKIS-PELISAASTPARTLRILARRLKQSGSTVLKEIESPRFQQD 174

Query: 176 LYVSFPVPTEIVTKVSLFTRAEPQYLFSNSSKNPRKHVLSGDKLLKWWLKIVDQIVVEDF 235
           LY+SF  P EI+TK+ LFTR   QYLF +SSKN +KH+L+G++L+KWW  I+D++++E F
Sbjct: 175 LYLSFTCPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEELMKWWGFILDRLLIECF 234

Query: 236 DNKTKATLQIPGEEKRVISNYLRNTAYQNWTVGDIFSESPQDIALYRIPLFPDDPKGRFL 295
            N T+A L+IPGE+   + +YLR   Y  W VGDIF+     +A+Y IPLFPDDPK RF+
Sbjct: 235 QNDTQAKLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENSLAVYNIPLFPDDPKARFI 294

Query: 296 DHLISDGRISKVNVLTFWTELQARQEFRLGSTVSVIGVSGKYIGRCN-EPERTDIIIPTS 354
             L  + R+ KV++ +FW ELQ RQEF+L  T SV+G+SG  +   +  P   D+I+P S
Sbjct: 295 HQLAEEDRLLKVSLSSFWIELQERQEFKLSVTSSVMGISGYSLATPSLFPSSADVIVPKS 354

Query: 355 KKNFKNLKNYIXXXXXXXXXXXXXXXMNIRDIFDMHYSTKMIKVIG-NHHKIATTKRPEI 413
           +K F+ +K YI                NIRD   +  +T +  + G   H+      P  
Sbjct: 355 RKQFRAIKKYITGEEYDTEEGAIEAFTNIRDFLLLRMATNLQSLTGKREHRERNQPVPAS 414

Query: 414 NTINVINTLSV 424
           N    INTL++
Sbjct: 415 N----INTLAI 421


>SPBC342.06c [S] KOG4534 Uncharacterized conserved protein
          Length = 369

 Score = 67.0 bits (162), Expect = 5e-11
 Identities = 50/153 (32%), Positives = 75/153 (48%), Gaps = 24/153 (15%)

Query: 190 VSLFTRAEPQYLFSNSSKNPRKHVLSGDKLLKWWLKIVDQIVVEDFDNKT---------- 239
           ++LF  A+ QYLF  S  N +KHVL+   LL+WW+  ++++     D++           
Sbjct: 114 LTLFAIAQGQYLFPESVDNGQKHVLNDSGLLRWWVNCLEKLRKYYTDSEAPNDSEKQKNS 173

Query: 240 ----KATLQIPGEEKRVISNYLRNTAYQNWTVGDIFSESPQDIALYRIPLFPDDPKGRFL 295
               KA L +PG E   I +YL N   ++W   +  +      A+  +P FPDDPK R+L
Sbjct: 174 TLLPKAYLFVPGLEN--IRSYLPN---RHWIESNAITTGK---AVEELPRFPDDPKCRYL 225

Query: 296 DHLISDGRISKVNVLTFWTELQARQEFRLGSTV 328
             L  +   S ++V  FW  L  RQE   G  V
Sbjct: 226 CELQDEK--SDMSVEEFWDTLTYRQECSSGKLV 256


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.320    0.137    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,246,433
Number of Sequences: 60738
Number of extensions: 991834
Number of successful extensions: 2173
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2166
Number of HSP's gapped (non-prelim): 3
length of query: 430
length of database: 30,389,216
effective HSP length: 109
effective length of query: 321
effective length of database: 23,768,774
effective search space: 7629776454
effective search space used: 7629776454
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)