ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI3410 suspect: %id D KOG4775 Cell cycle control, cell division, chromosome partitioning Uncharacterized protein SFI1 involved in G(2)-M transition
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI3410 1195799 1192884 -972
(972 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLL003w [D] KOG4775 Uncharacterized protein SFI1 involved in G(2... 281 3e-75
SPBPI059 [D] KOG4775 Uncharacterized protein SFI1 involved in G(... 77 2e-13
SPBC8D2.05c [D] KOG4775 Uncharacterized protein SFI1 involved in... 77 2e-13
>YLL003w [D] KOG4775 Uncharacterized protein SFI1 involved in G(2)-M
transition
Length = 946
Score = 281 bits (720), Expect = 3e-75
Identities = 211/864 (24%), Positives = 399/864 (45%), Gaps = 44/864 (5%)
Query: 108 NIHIPILQRLYNMIQVFLLRHGLTLDFLTIFQRYVNLLGESNVDVLQDKYLLNLQNELSK 167
+I + +++ LY I+ FL+ L+ FL IF+ YVN+L + ++ L+D+Y L++EL
Sbjct: 107 DISVEVIEDLYRQIEAFLVHFKLSRSFLQIFKNYVNILIQEGINPLRDEYFTILEDELKG 166
Query: 168 GYDFSTILQDIITAFLLKPENVIMKLAYFQFKTERLLLKKVFSSWQLKCSLNYNLSELEE 227
+ F++++++I+ FL+ P N + L+ ++ + +++ F+ W+ C LN +
Sbjct: 167 FFTFNSVIEEILEIFLIHPRNKFIALSLAEYTYAKNKIRRHFNHWKTVCELNEEANRFAN 226
Query: 228 IWNKCVKKKFFQRWVQTSDIKCREWVKNGDEVY-NQRLKTGFFDAWLDRIHQIDTKSQIA 286
V++ F W + +K + + E + N L F W+ + +S++A
Sbjct: 227 QAKLRVQEAVFYIWSDKT-LKYSQMANDEAESFRNTWLLFRSFQQWITLTQTLKEQSRLA 285
Query: 287 DNFFLDNTFANIKAKHERLTQCFNRTKNVNQKHLLQKSFKLWKLKMCEGHYNPTTMKRYL 346
D FL+ F I E + +K L+ +F +WKL+ E +Y+ ++R +
Sbjct: 286 DQAFLNKMFRKILKAQEHWKHLETVNTDNIKKIFLRTTFHIWKLRHKEINYHG--LERRI 343
Query: 347 LCKGFTEWKKKYAHEIYLRELATFSERNFHLS-PFLNKWISQYQKKEAYELKLIEKSNIF 405
F K+K + Y + +A R+F L +LNKW + + E L E N F
Sbjct: 344 ----FERIKQKVINYEYNKSIAE-KVRSFSLQRKYLNKWEKKNIENEDKLGALYELENKF 398
Query: 406 IKRRFFEMILLADKLVNVESKVQECNTESLQRYVLY-VWQKRFEERTNSHDYVVQQNTKI 464
IK++FF + + + E+ + ++L R V +W KRFE+ + + V + +
Sbjct: 399 IKQKFFRKLNRSFQHSQQEAIAKSKLNQTLLRCVFEKMWLKRFEDHLHLYSIVSLKEANL 458
Query: 465 VRFYFSQWK------LKSESYATANSHYTSLLKKKFIHSITLKCRAAQFNSSIKEAKYSK 518
V+ F WK LK+ Y+ N L K + S L+ + F K++ +
Sbjct: 459 VKRIFHSWKKLLYIDLKASDYSRTN------LLKSSLRSWKLEVKLKIFEQKCKKSIQAS 512
Query: 519 ALKLWRKKFELNALAQEF-DSLILKRYYNVVKNKQHSLKDLGNLSARCYDNDISKRHFMV 577
A + WRK+ + ++ E + +Y V K + + + + +++ Y+ + +
Sbjct: 513 AYRTWRKRIQYGKISSEHVKTAFCAKYLGVWKRRMLQMNSMNDEASKFYEEGLVNECLAI 572
Query: 578 WINRYKLLLSSQRKAEVFMKLRFITIIKRSINDTRMKTRLSIELSKGNSKMLVENLWKVW 637
W R + + K I +KR++ L +L+ ++ + + W
Sbjct: 573 WKERLIKTKELEDRYNFLCKTHAILTVKRTLMHIDNVHLLYTKLAPSMDRVKLSKAFLKW 632
Query: 638 KTRLESKRLLNLEMLLDKFEDRRSLQFNQKFLSAWMKRSRFYTVECASIAEQSSDMSSLK 697
+ K L +L +E + + + +AW R FYT EC A + K
Sbjct: 633 RKATRFKVRHKLNDILHVYEKSKERELQSQLFNAWRNRFCFYTEECNIQAISKRNYQLEK 692
Query: 698 NTMLQWTTKYSNIKLMHTEAAEIHDSISVSNAFDSILRKLEALQIQDSRLSMYVEEKQVK 757
+ ++ + I A E+ + + F L+ + + L KQ
Sbjct: 693 MVLKKFRERLLEIVKSEELADEVREEFVLVKTFYIWKTHLDEIFYMSTLLEQSEANKQFI 752
Query: 758 LLLKWTNKWSVKVLKLKRNEESVAMFRVRWNRANLRAIMSLWKEKAFSIVNYSSGNELAS 817
+ K+ WS++ LK+KRN+E+V +FR RW+RA +R ++ LWK + + SS
Sbjct: 753 ITSKFLKMWSLRFLKIKRNDETVEVFRHRWDRATVRGLLLLWKNR-----SDSSPKRRKD 807
Query: 818 YSPQNDELTFQTPMKSPNNAAVTIPGSTRVKRNKMDRIRNRFSRARGAIPSPIKTTNVLD 877
++ +++ +TP++S + A TIPGS R+K+++M+ +++ +SRAR AIPSP+K+++VLD
Sbjct: 808 FNLKHE---LKTPIRSDSQNASTIPGSERIKQHRMEAMKSHYSRARRAIPSPVKSSSVLD 864
Query: 878 STVKARLSDQRNTA--AKDTTGAQATYL-LSVNKRLAGKTRNISFNKIPETSLFGFPSPQ 934
ST K +++ + T T G Y + + K +I F +IP F +
Sbjct: 865 STAKKQINLESTTGLNGSPTRGKPLRYSPRRTTRNMPSKVDHIDFGRIPAVP-FSLSANS 923
Query: 935 PFEGPDPDKLKVDLDFL-EGDSLP 957
P K+ D+D++ E D P
Sbjct: 924 P-------KIDQDMDYIREHDKSP 940
>SPBPI059 [D] KOG4775 Uncharacterized protein SFI1 involved in G(2)-M
transition
Length = 726
Score = 76.6 bits (187), Expect = 2e-13
Identities = 129/621 (20%), Positives = 260/621 (41%), Gaps = 97/621 (15%)
Query: 222 LSELEEIWNKCVKKKFFQRWVQ-TSDIKCREWVKNGDEVYNQRLKTGFFDAW----LDRI 276
L + + ++ + K+F RWVQ +I+ R + + E Y L + W +R+
Sbjct: 77 LRKADAVYRAFIMLKYFPRWVQKLRNIQSR--IYSAGEAYRLILIGQMVNRWRQKTRERL 134
Query: 277 HQIDTKSQIADNFFLDNTFANIKAKHERLTQCFNRTKNVNQKHLLQKSFKLWKLKMCEGH 336
HQ K + N FL ++ K LT +NR ++ L ++ F W M +
Sbjct: 135 HQ---KLRFQKNAFL----LDVLLKWSALTVNWNRK---HEYMLKRRVFTNW---MSKRR 181
Query: 337 YNPTTMKR-------YLLCKGFTEWKKKYAHEIYLRELATFSERNFHLSPFLNKWISQYQ 389
N + +++ +L F WK + ++ AT E+ F N+W + Y
Sbjct: 182 RNESLIQKANVFFQHSVLAHSFKTWKA----NLSIKNAATLYEKKVVFMSF-NRWHANY- 235
Query: 390 KKEAYELKLIEKSNIFIKRRFFEMI-----LLADKLVNVESKVQECNTESLQRYVLYVWQ 444
E++ L L +K+ +F ++ ++ + L ++L ++ +K + +L + +L +W+
Sbjct: 236 --ESHVLDL-QKAELFSQKAYYSIALHKWKLRIEELSDLMNKADDLYEVNLLQRMLVLWR 292
Query: 445 KRFEERTNSHDYVVQQNTKIVRFYFSQWKLK----SESYATANSHYTSLLKKKFIHSITL 500
++ ++ + IV+ F +WK K ++ A++ + K+ ++ +
Sbjct: 293 RKATTYEKIDFWMDGHDVDIVKSAFFKWKNKFIKVDRNFELADTILQNTFLTKWRLAVRV 352
Query: 501 K---------------------CRAAQFNSSIKEAKYSKALKLWRKKFELN-ALAQEFDS 538
K +A F + A + W+ ++ L +E
Sbjct: 353 KVVQEKRNKDRLIVTFRFWYYLMKAKSFQHKRDHRLLTIAWEAWKDNYQRRIKLQKEAQE 412
Query: 539 LILKRYYNVVKNK----QHSLKDLGNLSARCYDNDISKRHFMV---WINRYKLLLSSQRK 591
L L R + K + SL D+ LS R + V W+ Y + Q+
Sbjct: 413 LQLTRIQKIAKQYLIKWRISLLDIKELSIRAEKLHTTNEIIGVWNQWVVSYNRKVGMQQN 472
Query: 592 AEVFMKLRFITII---KRSINDTRMKTRLS---IELSKGNSKMLVENLWKVWKTRLESKR 645
A++F K + + ++ R + +T M RL ++ + S+ LV W W R
Sbjct: 473 ADLFFKRKTLRVVFEHWRYLRETNMLLRLENKVVDFREVQSEKLVLRCWNAWVA-----R 527
Query: 646 LLNLEMLLDKFEDRRSLQFNQKFLSAWMKRSRFYTVECASIAEQSSDMSSLKNTMLQWTT 705
LL++ +LD E + + + AW R + E S A+Q + +++ M+ W
Sbjct: 528 LLSVSQMLDTAE-LSTQKVVVRAYEAWSFRYN-HQKEIMSQADQFYNERLMRSAMVFWRY 585
Query: 706 KYSNIKLMHTEA---AEIHDSISVSNAFDS---ILRKLEALQIQDSRLSMYVEEKQVKLL 759
+ IK + + ++ NAFD+ ++ ++ +L +Q R + E+K+ KLL
Sbjct: 586 QLRAIKFYFNISDWKRREFNRQTLRNAFDAWHLLMFRVTSLVMQADR---FHEQKKNKLL 642
Query: 760 LKWTNKWSVKVLKLKRNEESV 780
K+ KW ++ ++L + E S+
Sbjct: 643 GKYFRKW-LQRMQLNQEESSL 662
>SPBC8D2.05c [D] KOG4775 Uncharacterized protein SFI1 involved in G(2)-M
transition
Length = 840
Score = 76.6 bits (187), Expect = 2e-13
Identities = 129/621 (20%), Positives = 260/621 (41%), Gaps = 97/621 (15%)
Query: 222 LSELEEIWNKCVKKKFFQRWVQ-TSDIKCREWVKNGDEVYNQRLKTGFFDAW----LDRI 276
L + + ++ + K+F RWVQ +I+ R + + E Y L + W +R+
Sbjct: 191 LRKADAVYRAFIMLKYFPRWVQKLRNIQSR--IYSAGEAYRLILIGQMVNRWRQKTRERL 248
Query: 277 HQIDTKSQIADNFFLDNTFANIKAKHERLTQCFNRTKNVNQKHLLQKSFKLWKLKMCEGH 336
HQ K + N FL ++ K LT +NR ++ L ++ F W M +
Sbjct: 249 HQ---KLRFQKNAFL----LDVLLKWSALTVNWNRK---HEYMLKRRVFTNW---MSKRR 295
Query: 337 YNPTTMKR-------YLLCKGFTEWKKKYAHEIYLRELATFSERNFHLSPFLNKWISQYQ 389
N + +++ +L F WK + ++ AT E+ F N+W + Y
Sbjct: 296 RNESLIQKANVFFQHSVLAHSFKTWKA----NLSIKNAATLYEKKVVFMSF-NRWHANY- 349
Query: 390 KKEAYELKLIEKSNIFIKRRFFEMI-----LLADKLVNVESKVQECNTESLQRYVLYVWQ 444
E++ L L +K+ +F ++ ++ + L ++L ++ +K + +L + +L +W+
Sbjct: 350 --ESHVLDL-QKAELFSQKAYYSIALHKWKLRIEELSDLMNKADDLYEVNLLQRMLVLWR 406
Query: 445 KRFEERTNSHDYVVQQNTKIVRFYFSQWKLK----SESYATANSHYTSLLKKKFIHSITL 500
++ ++ + IV+ F +WK K ++ A++ + K+ ++ +
Sbjct: 407 RKATTYEKIDFWMDGHDVDIVKSAFFKWKNKFIKVDRNFELADTILQNTFLTKWRLAVRV 466
Query: 501 K---------------------CRAAQFNSSIKEAKYSKALKLWRKKFELN-ALAQEFDS 538
K +A F + A + W+ ++ L +E
Sbjct: 467 KVVQEKRNKDRLIVTFRFWYYLMKAKSFQHKRDHRLLTIAWEAWKDNYQRRIKLQKEAQE 526
Query: 539 LILKRYYNVVKNK----QHSLKDLGNLSARCYDNDISKRHFMV---WINRYKLLLSSQRK 591
L L R + K + SL D+ LS R + V W+ Y + Q+
Sbjct: 527 LQLTRIQKIAKQYLIKWRISLLDIKELSIRAEKLHTTNEIIGVWNQWVVSYNRKVGMQQN 586
Query: 592 AEVFMKLRFITII---KRSINDTRMKTRLS---IELSKGNSKMLVENLWKVWKTRLESKR 645
A++F K + + ++ R + +T M RL ++ + S+ LV W W R
Sbjct: 587 ADLFFKRKTLRVVFEHWRYLRETNMLLRLENKVVDFREVQSEKLVLRCWNAWVA-----R 641
Query: 646 LLNLEMLLDKFEDRRSLQFNQKFLSAWMKRSRFYTVECASIAEQSSDMSSLKNTMLQWTT 705
LL++ +LD E + + + AW R + E S A+Q + +++ M+ W
Sbjct: 642 LLSVSQMLDTAE-LSTQKVVVRAYEAWSFRYN-HQKEIMSQADQFYNERLMRSAMVFWRY 699
Query: 706 KYSNIKLMHTEA---AEIHDSISVSNAFDS---ILRKLEALQIQDSRLSMYVEEKQVKLL 759
+ IK + + ++ NAFD+ ++ ++ +L +Q R + E+K+ KLL
Sbjct: 700 QLRAIKFYFNISDWKRREFNRQTLRNAFDAWHLLMFRVTSLVMQADR---FHEQKKNKLL 756
Query: 760 LKWTNKWSVKVLKLKRNEESV 780
K+ KW ++ ++L + E S+
Sbjct: 757 GKYFRKW-LQRMQLNQEESSL 776
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.133 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,190,041
Number of Sequences: 60738
Number of extensions: 2469631
Number of successful extensions: 7090
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 7056
Number of HSP's gapped (non-prelim): 10
length of query: 972
length of database: 30,389,216
effective HSP length: 116
effective length of query: 856
effective length of database: 23,343,608
effective search space: 19982128448
effective search space used: 19982128448
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)