ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI3473 good T KOG2397 Signal transduction mechanisms Protein kinase C substrate, 80 KD protein, heavy chain
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI3473 1215760 1217745 662
(662 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR221w [T] KOG2397 Protein kinase C substrate 80 KD protein hea... 312 1e-84
CE15547 [T] KOG2397 Protein kinase C substrate 80 KD protein hea... 67 1e-10
SPCC825.02 [T] KOG2397 Protein kinase C substrate 80 KD protein ... 64 6e-10
7298396 [T] KOG2397 Protein kinase C substrate 80 KD protein hea... 64 6e-10
At5g56360 [T] KOG2397 Protein kinase C substrate 80 KD protein h... 62 4e-09
Hs4506077 [T] KOG2397 Protein kinase C substrate 80 KD protein h... 60 1e-08
At2g42390 [T] KOG2397 Protein kinase C substrate 80 KD protein h... 53 2e-06
>YDR221w [T] KOG2397 Protein kinase C substrate 80 KD protein heavy chain
Length = 702
Score = 312 bits (799), Expect = 1e-84
Identities = 208/686 (30%), Positives = 334/686 (48%), Gaps = 58/686 (8%)
Query: 18 IRGVPPENQSLYSPLPEDPTKWRCLDDTAKVIPYXXXXXXXXXXXXXXXEPGTNASQE-- 75
I GVP E Q LY D TKW CL+ V+ EPG+ A E
Sbjct: 28 IVGVPWEKQHLYDSNEPDLTKWHCLNHEDIVLDISQINDGVCDCPDGSDEPGSAACVEDI 87
Query: 76 -----------RALFYCNNEGFTPRNILNYKINDGVCDCCDCSDEYLR--EPFSRGKSCS 122
FYC+N+GF PR I ++ DG+CDCCDCSDE L E F G +CS
Sbjct: 88 FKSVAEGGGKVNKYFYCDNKGFIPRYIRKSEVADGICDCCDCSDELLSGYELFDAGSNCS 147
Query: 123 ELNDEFQRILNTELENYRAGVTALKKLKSK--TKDNTDDPNSAENVHFQIDGLTTYSVDL 180
+L +EF + + EL +YR G AL++L+ K TK+ + + S ++
Sbjct: 148 QLKNEFDIMASKELSSYREGKEALEELERKYGTKEEAITRGNCLE-----EDKEKASAEI 202
Query: 181 KTALNNVEENLQSLEESYRCKLFEE---SPQLFEYENLNVTFITDSVVSVFSHAQMLSNA 237
K N + EN LE+ R + F + P L+++E LN T + +++ F+ +S
Sbjct: 203 KVLSNRLSENRAKLEQ-LRGEYFNQLSHDPILYQFEQLNSTRLGSDILTSFTMVSRVSKG 261
Query: 238 FNELKFILNNLYETYNRKLNDKVVIKNMKQFAELEMKFKKS-FQPDGRLDGTQRDQINEY 296
+ ++ IL++L E Y LNDKVV N+K+F ++ + +K+ D ++D Q D + Y
Sbjct: 262 YQDIFKILSDLSEAYTPSLNDKVVNDNIKKFRKVRRRAEKAKINADSKIDDEQADNLYLY 321
Query: 297 LNEELPTFLFEGKSKYTPEIIVAKSNFIQRIINVKYKTKDLTLDGIKQFQQIADDIIENH 356
EE+P + +S+ T ++ KSNF+Q ++ K + L+ I++F+ I DDI +N+
Sbjct: 322 FTEEVPQIFLKRESENTLRYVIGKSNFVQALVEGKINYTNDILEYIREFRLIMDDISQNY 381
Query: 357 DINFQDGAVKSFTDSYMSFMSKYED--SLYAIDFPENFSDTFSELLLFIGKEAPNI---- 410
++NFQD VKS DSY +++ +Y + L E+ + SE+ F+ + AP +
Sbjct: 382 NVNFQDAGVKSAVDSYKNYLGEYGELAELEPAHPSESLLRSLSEVTSFVNENAPKVLPPD 441
Query: 411 AVDSGTE------GSDWGVMRIVQGAFDRMNFFSTSLQSYRSQIKFMXXXXXXXXXXXXX 464
AV+S + G+ + ++ ++N FS+ + +F
Sbjct: 442 AVESEQDTNSDHIGTSGDLRNKLKEILSKLNIFSSRKDLVSLEKRFRSCESQVSLLENEL 501
Query: 465 XXXXXXXXXQKLNDQGGEYSESQLIHKQVGTLLNNLK-----DFCVDSELNSYIYRLCYS 519
+KL D+ +E + + G L L+ +C+D L++Y+Y +C+
Sbjct: 502 KQKMDY---KKLLDE----TEDEGTNSTAGNLTELLELMGSQSYCLDDILDNYVYTICFQ 554
Query: 520 NDKG-GDIVQIENKPDGNKVVIGRYR--GFTIDTNSKYDVYLQSLKYRYPNTE--VGNHL 574
G I Q E+K DG KV+IGR++ GF +D N + Y + LK Y + N
Sbjct: 555 RPMTEGVIYQAEDKVDGKKVLIGRFKTSGFNVDLNM--EKYAEHLKATYDEKSDLISNLA 612
Query: 575 SSDTVEIGKEEXXXXXXXXXXXXXXXEYRGGTKCWDGPLRSATIQMRCAPDFKIESVSEP 634
+ + + EY G +CW+GP RSAT+ +RC+ FKI SV E
Sbjct: 613 AIQDDDGNMQHYVFGNLNELNNGLVLEYENGDQCWNGPRRSATVFVRCSDKFKIRSVHEA 672
Query: 635 TRCSYVFEMTGPLGCDPHFHYERPNW 660
T+C+Y+F++ GPLGC+ F YE P +
Sbjct: 673 TKCNYIFDVVGPLGCNKTFEYEPPKF 698
>CE15547 [T] KOG2397 Protein kinase C substrate 80 KD protein heavy chain
Length = 507
Score = 66.6 bits (161), Expect = 1e-10
Identities = 42/120 (35%), Positives = 55/120 (45%), Gaps = 9/120 (7%)
Query: 18 IRGVPPENQSLYSPLPEDPTKWRCLDDTAKVIPYXXXXXXXXXXXXXXXEPGTNASQERA 77
++GVP +LY P D +RCLD + ++ Y EPGT+A A
Sbjct: 27 VKGVPHSRLNLYQPSTTDT--FRCLDGSQTIL-YSQLNDDYCDCKDGSDEPGTSACGN-A 82
Query: 78 LFYCNNEGFTPRNILNYKINDGVCDCCDCSDEYLREPFSRGKSCSELNDEFQRILNTELE 137
FYC+N G I ++ND +CDCCD SDEY G C + DE R E E
Sbjct: 83 FFYCSNVGHKGNFIPTNRVNDKLCDCCDGSDEY-----DSGVDCPNICDELGRAARIEHE 137
>SPCC825.02 [T] KOG2397 Protein kinase C substrate 80 KD protein heavy chain
Length = 506
Score = 64.3 bits (155), Expect = 6e-10
Identities = 62/267 (23%), Positives = 111/267 (41%), Gaps = 21/267 (7%)
Query: 14 ASESIRGVPPENQSLYSPLPEDPTKWRCLDDTAKVIPYXXXXXXXXXXXXXXXEPGTNAS 73
A+ +RGV + LY P + W+CL K+I + EPGT+A
Sbjct: 23 AANDLRGVASDKSDLYKP--DAKGNWKCLGSD-KLISFNQVNDDYCDCPDGSDEPGTSAC 79
Query: 74 QERALFYCNNEGFTPRNILNYKINDGVCDCCDCSDEYLREPFSRGKSCSELNDEFQRILN 133
F+C N G+ I + +++D VCDCCD SDE L +C++ E+ L
Sbjct: 80 HN-GKFFCKNTGYISSYIPSNRVDDTVCDCCDGSDESL---IKCPNTCAQKAREYLATLE 135
Query: 134 TELENYRAGVTALKKLKSKTKDNTDDPNSAENVHFQIDGLTTYSVDLKTALNNVEENLQS 193
+ G+ ++ ++ TD+ V + ++ V + + E ++
Sbjct: 136 EHNRLVKNGLKIREQWALESAKKTDE------VKARYKEISDSLVAVSAEKTQLSEKVEK 189
Query: 194 LEESYRCKLFEESPQLFEYENLNVTFIT--DSVVSVFSHAQMLSNAFNELKFILNNLYET 251
++ S L E+ ++++L V ++ D + +L+N +EL LYET
Sbjct: 190 MKRS--TDLGAEAVLPLDFQDLRVALLSLVDERNEMQERLDILTNLLDELTL----LYET 243
Query: 252 YNRKLNDKVVIKNMKQFAELEMKFKKS 278
K I + + E E++ K S
Sbjct: 244 DKFDETMKEAILSFEDLKEQEIRRKVS 270
Score = 52.0 bits (123), Expect = 3e-06
Identities = 20/51 (39%), Positives = 29/51 (56%)
Query: 601 EYRGGTKCWDGPLRSATIQMRCAPDFKIESVSEPTRCSYVFEMTGPLGCDP 651
+Y G CW+GP RSA + + C + +I SV E +C Y+ +M P C P
Sbjct: 424 KYENGQSCWNGPHRSAIVTVECGVENEIVSVLEAQKCEYLIKMKSPAACSP 474
>7298396 [T] KOG2397 Protein kinase C substrate 80 KD protein heavy chain
Length = 548
Score = 64.3 bits (155), Expect = 6e-10
Identities = 39/105 (37%), Positives = 51/105 (48%), Gaps = 5/105 (4%)
Query: 20 GVPPENQSLYSPLPEDPTKWRCLDDTAKVIPYXXXXXXXXXXXXXXXEPGTNASQERALF 79
GVP SLY P + W CLD + + IP+ EPGT A + F
Sbjct: 34 GVPLAKASLYQPRAGE-NSWTCLDGS-RTIPFSHINDDYCDCADGSDEPGTAACPQ-GQF 90
Query: 80 YCNNEGFTPRNILNYKINDGVCDCCDCSDEYLREPFSRGKSCSEL 124
+C N+G P NI + ++ DG+CDCCD SDE E +C EL
Sbjct: 91 HCVNKGHQPVNIPSSQVQDGICDCCDGSDE--SETVGCPNTCLEL 133
Score = 60.5 bits (145), Expect = 8e-09
Identities = 26/50 (52%), Positives = 31/50 (62%)
Query: 601 EYRGGTKCWDGPLRSATIQMRCAPDFKIESVSEPTRCSYVFEMTGPLGCD 650
+Y G CW+GP RSA I + CA + KI +VSEP RC Y FE P CD
Sbjct: 484 KYTNGAACWNGPNRSAIINISCALEPKITAVSEPNRCEYYFEFETPAACD 533
>At5g56360 [T] KOG2397 Protein kinase C substrate 80 KD protein heavy chain
Length = 647
Score = 61.6 bits (148), Expect = 4e-09
Identities = 51/195 (26%), Positives = 80/195 (40%), Gaps = 13/195 (6%)
Query: 20 GVPPENQSLYSPLPEDPTKWRCLDDTAKVIPYXXXXXXXXXXXXXXXEPGTNASQERALF 79
G+ P+++ Y E K D +K EPGT+A F
Sbjct: 39 GISPQDEKYYKSSSEIKCK-----DGSKKFTKAQLNDDFCDCSDGTDEPGTSACPT-GKF 92
Query: 80 YCNNEGFTPRNILNYKINDGVCDCCDCSDEYLREPFSRGKSCSELNDEFQRILNTELENY 139
YC N G +P + + ++NDG+CDCCD SDEY S +C E + L ++E Y
Sbjct: 93 YCRNAGHSPVILFSSRVNDGICDCCDGSDEY-DGHVSCQNTCWEAGKAARENLKKKIETY 151
Query: 140 RAGVT----ALKKLKSKTKDNTDDPNSAENVHFQIDGLTTYSVDLKTALNNVE--ENLQS 193
G+ +++ K + + + ++ + GL D K + VE E LQ
Sbjct: 152 NQGLVIRRQEIEQAKVGLEKDAAELKKLKSEQKILKGLVDQLKDRKEQIEKVEEKERLQK 211
Query: 194 LEESYRCKLFEESPQ 208
+E K E + Q
Sbjct: 212 EKEEKEKKEAELAAQ 226
>Hs4506077 [T] KOG2397 Protein kinase C substrate 80 KD protein heavy chain
Length = 527
Score = 59.7 bits (143), Expect = 1e-08
Identities = 50/186 (26%), Positives = 79/186 (41%), Gaps = 15/186 (8%)
Query: 19 RGVPPENQSLYSPLPEDPTKWRCLDDTAKVIPYXXXXXXXXXXXXXXXEPGTNASQERAL 78
RGV N Y ++ + CLD +A IP+ EPGT A +
Sbjct: 21 RGVSLTNHHFY----DESKPFTCLDGSA-TIPFDQVNDDYCDCKDGSDEPGTAACPNGS- 74
Query: 79 FYCNNEGFTPRNILNYKINDGVCDCCDCSDEY----LREPFSRGKSCSELNDEFQRILNT 134
F+C N G+ P I + ++NDGVCDCCD +DEY + E + K E + Q++
Sbjct: 75 FHCTNTGYKPLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKE-RESLQQMAEV 133
Query: 135 ELENYRAGVTAL----KKLKSKTKDNTDDPNSAENVHFQIDGLTTYSVDLKTALNNVEEN 190
E +R + K + K K + +++ Q++ L T + + +E
Sbjct: 134 TREGFRLKKILIEDWKKAREEKQKKLIELQAGKKSLEDQVEMLRTVKEEAEKPEREAKEQ 193
Query: 191 LQSLEE 196
Q L E
Sbjct: 194 HQKLWE 199
Score = 55.1 bits (131), Expect = 3e-07
Identities = 20/49 (40%), Positives = 30/49 (60%)
Query: 601 EYRGGTKCWDGPLRSATIQMRCAPDFKIESVSEPTRCSYVFEMTGPLGC 649
+Y GT CW GP RS T+++ C + + S +EP+RC Y+ E+ P C
Sbjct: 463 KYEQGTGCWQGPNRSTTVRLLCGKETMVTSTTEPSRCEYLMELMTPAAC 511
>At2g42390 [T] KOG2397 Protein kinase C substrate 80 KD protein heavy chain
Length = 163
Score = 52.8 bits (125), Expect = 2e-06
Identities = 22/44 (50%), Positives = 30/44 (68%), Gaps = 1/44 (2%)
Query: 67 EPGTNASQERALFYCNNEGFTPRNILNYKINDGVCDCCDCSDEY 110
EPGT+A FYC N G +P+ + + ++ND +CDCCD SDEY
Sbjct: 52 EPGTSACPN-GKFYCRNIGSSPKFVYSSRVNDRICDCCDGSDEY 94
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.135 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,884,012
Number of Sequences: 60738
Number of extensions: 1598642
Number of successful extensions: 4561
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 4536
Number of HSP's gapped (non-prelim): 15
length of query: 662
length of database: 30,389,216
effective HSP length: 113
effective length of query: 549
effective length of database: 23,525,822
effective search space: 12915676278
effective search space used: 12915676278
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)