ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactVI3505   suspect: LH   S     KOG4473  Function unknown                                               Uncharacterized membrane protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI3505 1228739 1227831 -303 
         (303 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YLR220w [S] KOG4473 Uncharacterized membrane protein                  356   3e-98
At2g01770 [S] KOG4473 Uncharacterized membrane protein                156   4e-38

>YLR220w [S] KOG4473 Uncharacterized membrane protein
          Length = 322

 Score =  356 bits (913), Expect = 3e-98
 Identities = 189/322 (58%), Positives = 232/322 (71%), Gaps = 21/322 (6%)

Query: 1   MSVVAIKNAVSKLVAKNQEVPXXXXXXXXXXX-----XXXXXXXXHRYSASPQD------ 49
           MS+VA+KNAV  L+ K +                              S+S  D      
Sbjct: 1   MSIVALKNAVVTLIQKAKGSGGTSELGGSESTPLLRGSNSNSSRHDNLSSSSSDIIYGRN 60

Query: 50  --GDIESSKHTGGFDDYDNEDDGTS------GLFGKIDPRVMSDMIIGLSDGLTVPFALT 101
              D+E+S  + G D+  N D+G+       G F  +DPRV+SD+IIGLSDGLTVPFALT
Sbjct: 61  SAQDLENSPMSVGKDNR-NGDNGSDNEKANLGFFQSVDPRVISDLIIGLSDGLTVPFALT 119

Query: 102 AGLSSLGDSKLVITGGFAELISGAISMGLGGYLGAKSESDYYKSEVSQEKRKFYSNTQLI 161
           AGLSSLGD+KLVITGGFAELISGAISMGLGGYLGAKSESDYY +EV +EKRKFY N+ LI
Sbjct: 120 AGLSSLGDAKLVITGGFAELISGAISMGLGGYLGAKSESDYYHAEVKKEKRKFYDNSNLI 179

Query: 162 NHEIEDILMEMNPEFSENTVISFVKDMQRDPELMVDFIIKYGRGLDEPDDNRQWISALTI 221
           N EIEDIL+E+NP FS+ T++SF+KD+QR PELMVDFII+YGRGLDEP +NR+ ISA+TI
Sbjct: 180 NREIEDILLEINPNFSDETIVSFIKDLQRTPELMVDFIIRYGRGLDEPAENRELISAVTI 239

Query: 222 GGGYFAGGFVPLIPYFFVETVGTGLLISIALMTFTLFWFGYFKSQVSM-NDCTLQKKLLE 280
           GGGY  GG VPL+PYFFV  VGTGL+ SI +M  TLFWFGY K+++SM +  +  KK+ E
Sbjct: 240 GGGYLLGGLVPLVPYFFVSDVGTGLIYSIIVMVVTLFWFGYVKTKLSMGSGSSTSKKVTE 299

Query: 281 GLQMIAVGGTAAGAAWFFVKVI 302
           G++M+ VGG AAGAAWFFVK++
Sbjct: 300 GVEMVVVGGVAAGAAWFFVKLL 321


>At2g01770 [S] KOG4473 Uncharacterized membrane protein
          Length = 250

 Score =  156 bits (394), Expect = 4e-38
 Identities = 94/253 (37%), Positives = 141/253 (55%), Gaps = 17/253 (6%)

Query: 52  IESSKHTGGFDDYDNEDDGTSGLFGKIDPRVMSDMIIGLSDGLTVPFALTAGLSSLG-DS 110
           IE  K T    D+  E   T+G        ++ D+IIG+SDGLTVPFAL AGLS     S
Sbjct: 13  IEPEKQT--LLDHHTEKHFTAG-------EIVRDIIIGVSDGLTVPFALAAGLSGANASS 63

Query: 111 KLVITGGFAELISGAISMGLGGYLGAKSESDYYKSEVSQEKRKFYSNTQLINHEIEDILM 170
            +V+T G AE+ +GAISMGLGGYL AKSE D+Y  E+ +E+ +  +  +    E+ +IL 
Sbjct: 64  SIVLTAGIAEVAAGAISMGLGGYLAAKSEEDHYAREMKREQEEIVAVPETEAAEVAEILA 123

Query: 171 EMNPEFSENTVISFVKDMQRDPELMVDFIIKYGRGLDEPDDNRQWISALTIGGGYFAGGF 230
           +   E  E + +  V  ++++P+  +DF++++  GL++PD  R   SA TI   Y  GGF
Sbjct: 124 QYGIEPHEYSPV--VNALRKNPQAWLDFMMRFELGLEKPDPKRALQSAFTIAIAYVLGGF 181

Query: 231 VPLIPYFFVETVGTGLLISIALMTFTLFWFGYFKSQVSMNDCTLQKKLLEGLQMIAVGGT 290
           +PL+PY  +      ++ S+ +  F LF FGY K        T  K L    +   +G  
Sbjct: 182 IPLLPYMLIPHAMDAVVASVVITLFALFIFGYAKGHF-----TGSKPLRSAFETAFIGAI 236

Query: 291 AAGAAWFFVKVIE 303
           A+ AA+   KV++
Sbjct: 237 ASAAAFCLAKVVQ 249


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.319    0.139    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,637,570
Number of Sequences: 60738
Number of extensions: 701670
Number of successful extensions: 2011
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2004
Number of HSP's gapped (non-prelim): 2
length of query: 303
length of database: 30,389,216
effective HSP length: 106
effective length of query: 197
effective length of database: 23,950,988
effective search space: 4718344636
effective search space used: 4718344636
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)