ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI3563 good A KOG2071 RNA processing and modification mRNA cleavage and polyadenylation factor I/II complex, subunit Pcf11
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI3563 1246179 1244419 -587
(587 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR228c [A] KOG2071 mRNA cleavage and polyadenylation factor I/I... 456 e-128
SPAC4G9.04c [A] KOG2071 mRNA cleavage and polyadenylation factor... 181 4e-45
Hs7706224 [A] KOG2071 mRNA cleavage and polyadenylation factor I... 89 1e-17
7303127 [A] KOG2071 mRNA cleavage and polyadenylation factor I/I... 74 5e-13
At2g36480 [A] KOG2071 mRNA cleavage and polyadenylation factor I... 70 9e-12
CE02032 [A] KOG2071 mRNA cleavage and polyadenylation factor I/I... 66 1e-10
At1g66500 [A] KOG2071 mRNA cleavage and polyadenylation factor I... 56 1e-07
At5g43620 [A] KOG2071 mRNA cleavage and polyadenylation factor I... 54 9e-07
>YDR228c [A] KOG2071 mRNA cleavage and polyadenylation factor I/II complex
subunit Pcf11
Length = 626
Score = 456 bits (1172), Expect = e-128
Identities = 257/612 (41%), Positives = 354/612 (56%), Gaps = 76/612 (12%)
Query: 10 LEEFKTTLDGLTFNSRPIITTLTQLAEEHMSLAQEFVGLIENRLEKCVPSQKLFTFYVMD 69
+++F + L+ LTFNSRPIITTLT+LAEE++S AQ FV IE+R+EKC+P QKL+ FY +D
Sbjct: 9 VKDFNSILEELTFNSRPIITTLTKLAEENISCAQYFVDAIESRIEKCMPKQKLYAFYALD 68
Query: 70 SICKNAGSPYTIYFSKNLHKLFRKTYLLVDNQTRTKMISMFKTWMVPLPAGDLMFDHSSL 129
SICKN GSPYTIYFS+NL L+++TYLLVDN TRTK+I+MFK W+ P G +F+ S+L
Sbjct: 69 SICKNVGSPYTIYFSRNLFNLYKRTYLLVDNTTRTKLINMFKLWLNPNDTGLPLFEGSAL 128
Query: 130 NSIENFLIKASALHQKSATPPAIQQHTAHNGPAGSNITVKSLLSDIDKLALITQSKLVST 189
IE FLIKASALHQK+ TV LL DIDKL +T +L +
Sbjct: 129 EKIEQFLIKASALHQKNLQAML------------PTPTVPLLLRDIDKLTCLTSERLKNQ 176
Query: 190 PDDAKLQAKVAILKQLRQELQKERLAPEFLSQVHQQLKQIFAQEHXXXXXXXXXXXLPLQ 249
P+D KL+ K+ +L QL+QEL++E+L L QV QL+Q+F+Q+ L Q
Sbjct: 177 PNDEKLKMKLLVLSQLKQELKREKLTLNALKQVQMQLRQVFSQDQQVLQERMRYHELQQQ 236
Query: 250 NNSQFLPPSSQ--------------GSVNNDMSRGSLQANGNN-----------GSMFGG 284
Q Q GS + S ++ GNN S+FG
Sbjct: 237 QQQQQQQQQQQQQQQQQYHETKDMVGSYTQN-SNSAIPLFGNNSDTTNQQNSLSSSLFGN 295
Query: 285 SLQFNAPFLNQLTNSNRLNKLSSLVNMLREKELLHEPGEASITTLAAKLE-------PEF 337
+ ++ LNK+++L L+ + L++ P + SI TL KL
Sbjct: 296 ISGVES--FQEIEKKKSLNKINNLYASLKAEGLIYTPPKESIVTLYKKLNGHSNYSLDSH 353
Query: 338 DSSKTVQLPSYSLLQDILGDLEAQRQTQNLDILNASVLQLSQQFVLNDNHPITEKLVHFL 397
+ LP LL DIL D +A T N+D+LN LQLS+Q +L +N + L+H L
Sbjct: 354 EKQLMKNLPKIPLLNDILSDCKAYFATVNIDVLNNPSLQLSEQTLLQENPIVQNNLIHLL 413
Query: 398 YRAKPNKCSICGKRFGNTLEEKELEIQHLDWHFRVNKKIKGIQS-----------NTTTS 446
YR+KPNKCS+CGKRFGN+ EK L+ +HLDWHFR+N +IKG Q+ NTTT+
Sbjct: 414 YRSKPNKCSVCGKRFGNSESEKLLQNEHLDWHFRINTRIKGSQNTANTGISNSNLNTTTT 473
Query: 447 TKVIQSRNWYLDENQWADFKEEDIIAT-------------DIDTGAA-----EQQPTFAN 488
K IQSRNWYL ++QWA FK+++I +T +ID A E +
Sbjct: 474 RKNIQSRNWYLSDSQWAAFKDDEITSTKHKNDYTDPHANKNIDKSALNIHADENDEGSVD 533
Query: 489 NREDSTDAIYTKLRNKYIVVPDYATDMKFQCPICTESTYGKYDEDLGEWIWSNCVQVGDK 548
N S + ++R KY+VVP+ + DM F+CPIC E+ G YDE+ GEW+W N ++V K
Sbjct: 534 NTLGSDRSNELEIRGKYVVVPETSQDMAFKCPICKETVTGVYDEESGEWVWKNTIEVNGK 593
Query: 549 FFHATCYYEAAK 560
+FH+TCY+E ++
Sbjct: 594 YFHSTCYHETSQ 605
>SPAC4G9.04c [A] KOG2071 mRNA cleavage and polyadenylation factor I/II complex
subunit Pcf11
Length = 638
Score = 181 bits (458), Expect = 4e-45
Identities = 156/615 (25%), Positives = 254/615 (40%), Gaps = 97/615 (15%)
Query: 12 EFKTTLDGLTFNSRPIITTLTQLAEEHMSLAQEFVGLIENRLEKCVPSQKLFTFYVMDSI 71
++ + L+ LTFNS+PII TLT +A+E+ A V IE ++KC P+ KL Y++DSI
Sbjct: 7 DYLSALEDLTFNSKPIIHTLTYIAQENEPYAISIVNAIEKHIQKCPPNCKLPALYLLDSI 66
Query: 72 CKNAGSPYTIYFSKNLHKLFRKTYLLVDNQTRTKMISMFKTW------------------ 113
KN G+PYT +F +L F Y +V+ + R K+ + TW
Sbjct: 67 SKNLGAPYTYFFGLHLFSTFMSAYTVVEPRLRLKLDQLLATWKQRPPNSSSLEPVFSPIV 126
Query: 114 ------------------MVPLPAGDLMFDHS-----SLNSIENFLIKASALHQKSATPP 150
PL A + S ++NS +F AS+ + P
Sbjct: 127 TAKIENALLKYKSTILRHQSPLLANTSISSFSAPIDANINSYSSFSDPASSYKPSLPSVP 186
Query: 151 AIQQHTAHNGPAGSNITVKSLLSDIDKLALITQSKLVSTPDDAKLQAKVAILKQLRQELQ 210
QH + P+ IT+ SLLSD++++ + Q++ + P D + + IL QL+ L
Sbjct: 187 FGFQHISGTSPSPGFITLDSLLSDVNRMIVTEQARFIKNPYDNMAKKRFEILLQLKNVLS 246
Query: 211 KERLAPEFLSQVHQQLKQIF-----AQEHXXXXXXXXXXXLPLQNNSQFLPPSSQGSVNN 265
L + L + QL Q+ + L +++ F+ PS ++
Sbjct: 247 SSALPYDQLLAIKNQLAQLEKPASPSTSSVATSAPSVPSALSSISSTPFMKPSIPSTIPT 306
Query: 266 DMSRGSLQANGNNGSMFGGSLQFNAPFLNQLTNSNRLNKLSSLVNMLREKELLHEPGEAS 325
S S + +++ S++ + S L L +S
Sbjct: 307 IPSAYSASVSSQP--------PLTHSYVHPGPQSHKYSLSSGPPASLYNANALTPEESSS 358
Query: 326 ITTL-----AAKLEPEFDSSKTVQLPSYSLLQDILGDLEAQRQTQNLDILNASVLQLSQQ 380
I +L AA L P K+ Q P S EA T ++D+ +S
Sbjct: 359 IDSLFANLQAAGLVPPSAGGKS-QGPQASC-------TEAVSLTADIDLSKSS------- 403
Query: 381 FVLNDNHPITEKLVHFLYRAKPNKCSICGKRFGNTLEEKELEIQHLDWHFRVNKKIKGIQ 440
L P KL LY N+C+ CG+R+GN E ++ +H DWHFR+NK+I+
Sbjct: 404 --LATPRP---KLSSLLYENYSNQCANCGRRYGNDPESRKELDKHSDWHFRINKRIR--- 455
Query: 441 SNTTTSTKVIQSRNWYLDENQWADFKEEDIIATDIDTGAAEQQPTFANNREDSTDAIYTK 500
S+ +R W++ E +W + KEE+ + T+ EQ R+ +++
Sbjct: 456 ----ESSLHGINRCWFVMEEEWVNSKEEEDLITETAQEIEEQ-------RQKQMESV--- 501
Query: 501 LRNKYIVVPDYATDMKFQCPICTESTYGKYDEDLGEWIWSNCVQVGDKFFHATCYYEAAK 560
R++Y++ P CPIC E + E+ W++ N V+ + FHATC E
Sbjct: 502 -RSQYVLTPLDPIAASEPCPICQEKFQSVWHEEAEVWVFMNAVEEEGRIFHATCLQEVRP 560
Query: 561 XXXXXXXXXXXXXNL 575
NL
Sbjct: 561 SENKHSNTNTSTQNL 575
>Hs7706224 [A] KOG2071 mRNA cleavage and polyadenylation factor I/II complex
subunit Pcf11
Length = 725
Score = 89.4 bits (220), Expect = 1e-17
Identities = 68/216 (31%), Positives = 107/216 (49%), Gaps = 13/216 (6%)
Query: 8 NALEEFKTTLDGLTFNSRPIITTLTQLAEEHMSLAQEFVGLIENRLEKCVPSQKLFTFYV 67
+A +++++L+ LTFNS+P I LT LAEE++ A+E V LIE + K S+KL Y+
Sbjct: 17 DACRDYQSSLEDLTFNSKPHINMLTILAEENLPFAKEIVSLIEAQTAKAPSSEKLPVMYL 76
Query: 68 MDSICKNAGSPYTIYFSKNLHKLFRKTYLLVDNQTRTKMISMFKTWMVPLPAGDLMFDHS 127
MDSI KN G Y F+KNL F + VD TR + + TW P L
Sbjct: 77 MDSIVKNVGREYLTAFTKNLVATFICVFEKVDENTRKSLFKLRSTWDEIFPLKKLYALDV 136
Query: 128 SLNSIENFL--------IKASALH--QKSATPPAIQQHTAHNGPAGSNITVKSLLSDIDK 177
+NS++ + S++H K + T + +I+ ++ DI K
Sbjct: 137 RVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEEPSTPGTVVSSPSISTPPIVPDIQK 196
Query: 178 LALITQSKLVSTPDDAKLQAKVAILKQLRQELQKER 213
+TQ +L+ AK Q ++ L+Q + EL+ E+
Sbjct: 197 --NLTQEQLIRQQLLAK-QKQLLELQQKKLELELEQ 229
>7303127 [A] KOG2071 mRNA cleavage and polyadenylation factor I/II complex
subunit Pcf11
Length = 1945
Score = 74.3 bits (181), Expect = 5e-13
Identities = 50/156 (32%), Positives = 75/156 (48%), Gaps = 18/156 (11%)
Query: 404 KCSICGKRFGNTLEEKELEIQHLDWHFRVNKKIKGIQSNTTTSTKVIQSRNWYLDENQWA 463
+CS CG RF E+ QHLDWHFR N++ + ST+ SR WY D N W
Sbjct: 1359 QCSSCGVRFPP--EQTIKYSQHLDWHFRQNRRERD-------STRKATSRKWYYDLNDWR 1409
Query: 464 DFKE-EDIIATDIDTGAAEQQPTFANNREDSTDAIYTKLRNKYIVVPDYAT---DMKFQC 519
++E ED+ + + A+ QP ++ ++ R+ VP A D+ C
Sbjct: 1410 QYEEIEDVEEREKNFLEAQGQPGGVEALDE-----LSQQRSLDSPVPTCAAGRDDVDHCC 1464
Query: 520 PICTESTYGKYDEDLGEWIWSNCVQVGDKFFHATCY 555
+C E Y+E+L EW + ++V DK +H CY
Sbjct: 1465 DMCHEKFEQFYNEELEEWHLRSAIRVEDKIYHPLCY 1500
Score = 62.4 bits (150), Expect = 2e-09
Identities = 77/324 (23%), Positives = 136/324 (41%), Gaps = 50/324 (15%)
Query: 11 EEFKTTLDGLTFNSRPIITTLTQLAEEHMSLAQEFVGLIENRLEKCVPSQKLFTFYVMDS 70
EE+ ++L L NS+P+I LT LAEE+++ A V ++E + + P KL Y++DS
Sbjct: 17 EEYLSSLQDLNCNSKPLINMLTMLAEENINYAHIIVKVVEYYISQVAPEFKLPILYLIDS 76
Query: 71 ICKNAGSPYTIYFSKNLHKLFRKTYLLVDN-------QTRTKMISMFKTWMVPLPAGDLM 123
I KN S Y F + + +F + V + + R +M ++ +TW P +
Sbjct: 77 IVKNVKSSYVQLFGQCIVNIFLHAFESVQHSQSQVLEKVRERMYALRQTWNEVFPPSKMY 136
Query: 124 FDHSSLNSIE-NFLIKASALHQKSATPPAIQQHTAHNGPA-----GSNITVKSLLSDIDK 177
+ ++ N+ I A K PAI + P N T+ S + +I
Sbjct: 137 ALDVKVKRLDNNWPITAKQPTNKIHVNPAIHVNPDFLKPGLVPGMPGNPTITSDMEEI-- 194
Query: 178 LALITQSKLVSTPDDAKLQAKVAILKQLRQ---ELQKERLAPEFLSQVHQQLKQIFAQEH 234
LQAK L +L++ EL+ E+ + L + +QL Q
Sbjct: 195 -----------------LQAKTRELLELKKRKLELELEQ-TKKHLEEQERQLTQ------ 230
Query: 235 XXXXXXXXXXXLPLQNNSQFLPPSSQGSVNNDMSRGSLQANGNNGSMFGGSLQFNAPFLN 294
+P + PP + + R A G G++ G + N P
Sbjct: 231 TTDAMVGAPIIMPAPTPAAICPPITDPGIAVHNQR----APGVMGNV--GPVMHNMPVAP 284
Query: 295 QL-TNSNRLNKLS-SLVNMLREKE 316
QL N +++ ++ +L+N +R+++
Sbjct: 285 QLFANKPKVHPVNPALLNSVRQRD 308
>At2g36480 [A] KOG2071 mRNA cleavage and polyadenylation factor I/II complex
subunit Pcf11
Length = 984
Score = 70.1 bits (170), Expect = 9e-12
Identities = 44/119 (36%), Positives = 60/119 (49%), Gaps = 15/119 (12%)
Query: 10 LEEFKTTLDGLTFNSRPIITTLTQLAEEHMSLAQEFVGLIENRLEKCVPSQKLFTFYVMD 69
+ ++K+ L LTFNS+PIIT LT +A E++ A+ V I N + + QKL T Y++D
Sbjct: 90 VNQYKSALAELTFNSKPIITNLTIIAGENVHAAKAVVTAICNNILEVPSDQKLPTLYLLD 149
Query: 70 SICKNAGSPYTIYFSKNLH---------------KLFRKTYLLVDNQTRTKMISMFKTW 113
SI KN G Y YF L K+F K Y VD + M +F TW
Sbjct: 150 SIVKNIGRDYIKYFGARLPEVCKFISDHPLFTSLKVFVKAYRQVDPPMHSNMRHLFGTW 208
>CE02032 [A] KOG2071 mRNA cleavage and polyadenylation factor I/II complex
subunit Pcf11
Length = 823
Score = 66.2 bits (160), Expect = 1e-10
Identities = 38/112 (33%), Positives = 56/112 (49%), Gaps = 2/112 (1%)
Query: 4 EDGSNALEEFKTTLDGLTFNSRPIITTLTQLAEEHMSLAQEFVGLIENRLEKCVPSQKLF 63
E +A +++ TL L N++ I LT LA++ A + V +IE L C PSQKL
Sbjct: 2 ESVESAARDYRETLAELRNNNKTQINLLTILADDFKKAAPQIVEVIERHLTTCSPSQKLL 61
Query: 64 TFYVMDSICKNAGSP--YTIYFSKNLHKLFRKTYLLVDNQTRTKMISMFKTW 113
YV DSI KN P Y F++ + +F + D + RT + + TW
Sbjct: 62 VMYVCDSILKNVKKPNDYDALFARKIVSMFEHAFRQGDERIRTSLYRIRVTW 113
>At1g66500 [A] KOG2071 mRNA cleavage and polyadenylation factor I/II complex
subunit Pcf11
Length = 416
Score = 56.2 bits (134), Expect = 1e-07
Identities = 51/215 (23%), Positives = 90/215 (41%), Gaps = 34/215 (15%)
Query: 351 LQDILGDLEAQRQTQNLDILNASVLQLSQQF----VLNDNHPITEKLVHFLYRAKPNKCS 406
L D+L L +++ + L+ N+ L + F LN H E ++ LY P +CS
Sbjct: 201 LTDLLSLLNNEKEKKTLEASNSDSLPVGLSFDNPSSLNVRH---ESVIKSLYSDMPRQCS 257
Query: 407 ICGKRFGNTLEEKELEIQHLDWHFRVNKKIKGIQSNTTTSTKVIQSRNWYLDENQW--AD 464
CG RF + +E +H+DWH R N+ +K + T + +SR W + W A
Sbjct: 258 SCGLRF----KCQEEHSKHMDWHVRKNRSVK---TTTRLGQQPKKSRGWLASASLWLCAA 310
Query: 465 FKEEDIIATDIDTGAAEQQPTFANNREDSTDAIYTKLRNKYIVVPDYATDMKFQCPICTE 524
E + ++ + E Q + E K ++VP A + + C +C E
Sbjct: 311 TGGETV---EVASFGGEMQKKKGKDEEP-----------KQLMVP--ADEDQKNCALCVE 354
Query: 525 STYGKYDEDLGEWIWSNCVQV--GDKFFHATCYYE 557
+ + +W++ + V + + H C E
Sbjct: 355 PFEEFFSHEDDDWMYKDAVYLTKNGRIVHVKCMPE 389
>At5g43620 [A] KOG2071 mRNA cleavage and polyadenylation factor I/II complex
subunit Pcf11
Length = 410
Score = 53.5 bits (127), Expect = 9e-07
Identities = 49/213 (23%), Positives = 83/213 (38%), Gaps = 29/213 (13%)
Query: 351 LQDILGDLEAQRQTQNLDILNASVLQLSQQF----VLNDNHPITEKLVHFLYRAKPNKCS 406
L D+L L +++ + + N L + F LN H E ++ LY P +C+
Sbjct: 194 LTDLLSLLNNEKEKKTSEASNNDSLPVGLSFDNPSSLNVRH---ESVIKSLYSDMPRQCT 250
Query: 407 ICGKRFGNTLEEKELEIQHLDWHFRVNKKIKGIQSNTTTSTKVIQSRNWYLDENQWADFK 466
CG RF + +E +H+DWH R N+ +K + T + +SR W + W
Sbjct: 251 SCGVRF----KCQEEHSKHMDWHVRKNRSVK---TTTRLGQQPKKSRGWLASASLWLCAP 303
Query: 467 EEDIIATDIDTGAAEQQPTFANNREDSTDAIYTKLRNKYIVVPDYATDMKFQCPICTEST 526
G E Q N +D K +VP A + + C +C E
Sbjct: 304 TGGGTVEVASFGGGEMQ---KKNEKDQV--------QKQHMVP--ADEDQKNCALCVEPF 350
Query: 527 YGKYDEDLGEWIWSNCVQV--GDKFFHATCYYE 557
+ + +W++ + V + + H C E
Sbjct: 351 EEFFSHEADDWMYKDAVYLTKNGRIVHVKCMPE 383
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.131 0.376
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,251,775
Number of Sequences: 60738
Number of extensions: 1396404
Number of successful extensions: 7931
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 7908
Number of HSP's gapped (non-prelim): 14
length of query: 587
length of database: 30,389,216
effective HSP length: 112
effective length of query: 475
effective length of database: 23,586,560
effective search space: 11203616000
effective search space used: 11203616000
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)