ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactVI3780   suspect: LH   P     KOG1418  Inorganic ion transport and metabolism                         Tandem pore domain K+ channel

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI3780 1317212 1315329 -628 
         (628 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YJL093c [P] KOG1418 Tandem pore domain K+ channel                     353   6e-97
CE00160 [P] KOG1418 Tandem pore domain K+ channel                      52   4e-06

>YJL093c [P] KOG1418 Tandem pore domain K+ channel
          Length = 691

 Score =  353 bits (905), Expect = 6e-97
 Identities = 187/484 (38%), Positives = 278/484 (56%), Gaps = 20/484 (4%)

Query: 25  LTESVGFKAERLEILSIDPSSKWFIVWFAVSCYFPVVTACLGPLSNMISVACAVDHWR-- 82
           L +++ F+ ER+ I++ +PSS  F+ WF VSCYFPV+TACLGP++N IS+AC V+ WR  
Sbjct: 49  LKDALRFRDERVSIINAEPSSTLFVFWFVVSCYFPVITACLGPVANTISIACVVEKWRSL 108

Query: 83  ------IDPHTGHQ---------VSDPPGIRVLNIISLVSGVIANFVLLLNFTGKLAYVK 127
                  +P +            V DPPGI  +NIISLV G  +N +L+L+F+ KL Y+K
Sbjct: 109 KNNSVVTNPRSNDTDVLMNQVKTVFDPPGIFAVNIISLVLGFTSNIILMLHFSKKLTYLK 168

Query: 128 AQIICISGWXXXXXXXXXXXXXXXXXYFEDGYQRSIGYWYAVITACLYLLCLILQSIHVS 187
           +Q+I I+GW                      Y ++IG+W+A I++ LYL+C I+ +IH  
Sbjct: 169 SQLINITGWTIAGGMLLVDVIVCSLNDMPSIYSKTIGFWFACISSGLYLVCTIILTIHFI 228

Query: 188 GYYKKKYPATFNLLDNERLLMKFTFVFAIWFLWGAAMFSQLLGQTYGASLYFCVVSVLTI 247
           GY   KYP TFNLL NER +M +T + ++W +WGA MFS LL  TYG +LYFC VS+LT+
Sbjct: 229 GYKLGKYPPTFNLLPNERSIMAYTVLLSLWLIWGAGMFSGLLHITYGNALYFCTVSLLTV 288

Query: 248 GLGDIFPDNTASEVMXXXXXXXXXXXXXXXXXXXXXTMKSSSGPIFFFYSVEIQREKVYQ 307
           GLGDI P +  +++M                      ++ SSGPIFFF+ VE  R K ++
Sbjct: 289 GLGDILPKSVGAKIMVLIFSLSGVVLMGLIVFMTRSIIQKSSGPIFFFHRVEKGRSKSWK 348

Query: 308 KYLANPALEFTDHDAYETIQSIRKRSKMLQRRNSXXXXXXXXXXXXXXGALVLYFSESWT 367
            Y+ + +   ++ +A++ ++ IR+ +   Q   S              GALV  F+E+W+
Sbjct: 349 HYM-DSSKNLSEREAFDLMKCIRQTASRKQHWFSLSVTIAIFMAFWLLGALVFKFAENWS 407

Query: 368 YFEAFYFCLLSVLTIGYGVPSPVSGCGRAFYVIWCLGAVPLMTVLISTVGDWIYEISKSI 427
           YF   YFC L +LTIGYG  +P +G GRAF+VIW LGAVPLM  ++STVGD +++IS S+
Sbjct: 408 YFNCIYFCFLCLLTIGYGDYAPRTGAGRAFFVIWALGAVPLMGAILSTVGDLLFDISTSL 467

Query: 428 DNNILEYFHLGNLYRMAKYCVGKVREQFVVKTSSMATVDQTAESETLPDEQEFENYEQRV 487
           D  I E F+  N  +   +   +    F+V T  +     TA+ +   +    ++ +   
Sbjct: 468 DIKIGESFN--NKVKSIVFNGRQRALSFMVNTGEIFEESDTADGDLEENTTSSQSSQISE 525

Query: 488 ISDN 491
            +DN
Sbjct: 526 FNDN 529


>CE00160 [P] KOG1418 Tandem pore domain K+ channel
          Length = 335

 Score = 51.6 bits (122), Expect = 4e-06
 Identities = 25/75 (33%), Positives = 44/75 (58%)

Query: 363 SESWTYFEAFYFCLLSVLTIGYGVPSPVSGCGRAFYVIWCLGAVPLMTVLISTVGDWIYE 422
           +E+WT+  + +F +  V TIGYG P PV+  GR + +++ L  +PL  V I+ +G ++ E
Sbjct: 25  TETWTFSSSIFFAVTVVTTIGYGNPVPVTNIGRIWCILFSLLGIPLTLVTIADLGKFLSE 84

Query: 423 ISKSIDNNILEYFHL 437
               +  N L+  +L
Sbjct: 85  HLVWLYGNYLKLKYL 99


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.324    0.137    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,718,623
Number of Sequences: 60738
Number of extensions: 1087040
Number of successful extensions: 3462
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3451
Number of HSP's gapped (non-prelim): 5
length of query: 628
length of database: 30,389,216
effective HSP length: 113
effective length of query: 515
effective length of database: 23,525,822
effective search space: 12115798330
effective search space used: 12115798330
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)