ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI3786 good L KOG2108 Replication, recombination and repair 3'-5' DNA helicase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI3786 1317691 1320882 1064
(1064 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL092w [L] KOG2108 3'-5' DNA helicase 596 e-170
SPAC4H3.05 [L] KOG2108 3'-5' DNA helicase 257 6e-68
YOL095c [L] KOG2108 3'-5' DNA helicase 86 2e-16
>YJL092w [L] KOG2108 3'-5' DNA helicase
Length = 1174
Score = 596 bits (1536), Expect = e-170
Identities = 350/833 (42%), Positives = 497/833 (59%), Gaps = 70/833 (8%)
Query: 10 LSNLNDAQRKAVIYDYTKALQIIAGPGTGKTKVLTARFAYLVLSQKINAEEIIMTTFTRK 69
+S LN QR A ++DYT+ LQ+IAGPGTGKTKVLT+R AYL+L I+ +II+TTFT K
Sbjct: 12 VSQLNTQQRAAALFDYTRGLQVIAGPGTGKTKVLTSRVAYLILHHHIHPRDIIVTTFTNK 71
Query: 70 AAEEMKERLVPVLVSNGISPHGLKIGTFHSICWKLLKEKGFLIGKEKIERATTEK-IKSI 128
AA EMKERL +L G++ L IGTFHSIC K+L G L+ +K R EK I I
Sbjct: 72 AANEMKERLQEMLRGAGVNISELLIGTFHSICLKILYRFGHLVDLQKDWRIIDEKEIDVI 131
Query: 129 LEELIVEMPDQIRDYACTKSYKNEVSLCRLKNN--QWQVHPDMILKRISELKADAITPEK 186
L+++I ++PDQIRDYA S +V+LC N +W +HP +I K+IS+LK++AI PE+
Sbjct: 132 LDDMIEKVPDQIRDYA--SSITRKVNLCMPSKNGDEWTIHPKLIKKQISKLKSNAILPEE 189
Query: 187 YEQQAIFDEALLHFYQQYQAKLSKEHLIDFDDVMLYSFELLSKHRVWKNIKHVLVDEFQD 246
Y + D AL +FYQ YQ++LSK++ +DFDD+++Y+F LL++ RV NIKHVLVDEFQD
Sbjct: 190 YILDSNHDAALGYFYQIYQSELSKKNTLDFDDLLMYTFRLLTRVRVLSNIKHVLVDEFQD 249
Query: 247 TNLLQIHLIFQFARGSHHSCEGVTVVGDPDQGIYGFRSALSHNFQTMIELCPIEYDQIVL 306
TN +Q+ L+F FA+G+HH G+T+VGDPDQ IY FR+AL+HNF M CPIEY I+L
Sbjct: 250 TNGIQLDLMFLFAKGNHHLSRGMTIVGDPDQSIYAFRNALAHNFLEMGRKCPIEYSTIIL 309
Query: 307 KENYRSAQAILDISETVIKQQKSGRDNREPLRAQFVTDNKPVYMSFPSDDTEAYTITKEI 366
ENYRS+Q IL+ SE +I QQ GR NR PLRAQF D PVYM+FP+ EA ++ +E+
Sbjct: 310 VENYRSSQKILNTSEILITQQNKGRQNRAPLRAQFDLDFPPVYMNFPAYFLEAPSLVREL 369
Query: 367 LYLKAIPGLFKYKDFAILVRLKRSLRTFENALIAHKIPYVIQKGYTFWELKECKAIIRYM 426
LYLKA+P LF + DFAILVR +R ++ E+ALI H+IPY I +G++FW+ KE +A++ +
Sbjct: 370 LYLKALPNLFTFNDFAILVRQRRQIKRIESALIEHRIPYKIIRGHSFWDSKETRAMLNLL 429
Query: 427 SMICNDDDWESLKRTINYPNRGVGETTVGKIAAAFEKESTAEKNETAFSFLQKIARDSVK 486
+I + +D ++ ++ YP RG+G T KI A + T + + F L+ I+ +
Sbjct: 430 KLIFSPNDKHAILASLLYPARGLGPATGEKIKNALD---TLATDVSCFQILKDISSKKIM 486
Query: 487 INISNSSMIGIKDYVNIIEQVRKFFSEIYSDAGKSTIADAFEKFYVLTGLRGQ--YKPEK 544
++I I D++++IE + ++D F+K Y L+GL+ + YK K
Sbjct: 487 LDIPTKGRSVIADFISMIENCQLLLQSTLLGG----LSDLFDKLYELSGLKYEYLYKDGK 542
Query: 545 KSGSIASDDQSTEKEAH----RHLNVLNVKNYFSTF-------------KIPLGDGDGEP 587
K +DQ + E + RH N+ +KNYF I + EP
Sbjct: 543 K-----KNDQLEKSEPNLLNARHKNIELLKNYFLALLSKSESSDKEKNEAIKAATDEAEP 597
Query: 588 ---RIVDLIGFCHEFVSFTDLYT-----FNEDNDNDVKDDHETKDAVVLSTIHASKGLEW 639
+++ + F + L++ +++ D K E V +STIH +KGLEW
Sbjct: 598 IENKVITPKEYLRNFFNSLSLHSDAAEEEESESNKDAKIKREKNGFVTISTIHGAKGLEW 657
Query: 640 PVVFVPQCVEGLLPSYYK-DSSDMPAPDEDSDENMAVGDAYNEPTD--SQDDNRDGXXXX 696
PVVF+P C EG++P + D D DE+ D+ + DA + T S +D+ D
Sbjct: 658 PVVFIPGCEEGIIPCVFNDDKKDESEEDEEEDQENSKKDASPKKTRVLSVEDSID----- 712
Query: 697 XXXXXXXXXXFSEERRMFFVALTRAKYLLY----ITTVEKEATSFKSRFLKNEVIELCDD 752
EERRMFFVA TRAKYLLY +T + + SRFL ++I+ D
Sbjct: 713 ------------EERRMFFVAQTRAKYLLYLSNTVTVEDVDRPRIASRFLTTDLIKAMSD 760
Query: 753 TLKCFESIEHLFKLYYTLKVQIPNQD--RISYDQLIKDYYNYGFNSRQLLFWK 803
+ K FES + KLY L + P +D S DQL KDY + N R+ + W+
Sbjct: 761 SQKLFESTNSIKKLYRILNKKPPAEDDKLFSLDQLRKDYNQFIENRRERMIWQ 813
>SPAC4H3.05 [L] KOG2108 3'-5' DNA helicase
Length = 887
Score = 257 bits (657), Expect = 6e-68
Identities = 223/741 (30%), Positives = 358/741 (48%), Gaps = 108/741 (14%)
Query: 9 YLSNLNDAQRKAVIYDYTKALQIIAGPGTGKTKVLTARFAYLVLSQKINAEEIIMTTFTR 68
YL LN+ QR +V + K QI+AGPG+GKT+VLTAR AYL+ I AE++I+ TFT
Sbjct: 7 YLKFLNEEQRISVQSPH-KYTQILAGPGSGKTRVLTARVAYLLQKNHIAAEDLIIATFTN 65
Query: 69 KAAEEMKERLVPVLVSNGISPHGLKIGTFHSICWKLLKEKG--------FLIGKEKIERA 120
KAA E+K R+ +L ++ S L GTFHSI +K L + G +LI +A
Sbjct: 66 KAANEIKLRIEAILGNSEASK--LISGTFHSIAYKYLVKYGKHIGLSSNWLIADRNDTQA 123
Query: 121 TTEKIKSILEELIVEMPDQIRDYACTKSYKNEVS-LCRLKNNQWQVHPDMILKRISELKA 179
+++ L++ + IR T +N ++ + +LK+N V P M ++S +
Sbjct: 124 IMKRLLDSLKKAKNPIASGIRGQELTP--QNALNRITKLKSNGLLVKPGM--DQLSLING 179
Query: 180 DAITPEKYEQQAIFDEALLHFYQQYQAKLSKEHLIDFDDVMLYSFELLSKH-RVWKNIKH 238
P++ + + Y+ YQ L K +L DFDD++L LL K +NIKH
Sbjct: 180 LEEPPKELQ-----SHQSVELYRLYQTSLWKNNLADFDDLLLNFILLLQKQPDCVRNIKH 234
Query: 239 VLVDEFQDTNLLQIHLIFQFARGSHHSCEGVTVVGDPDQGIYGFRSALSHNFQTMIELCP 298
+L+DEFQDT+ +Q L+ A + +T+VGDPDQ IYGFRSA N M E
Sbjct: 235 ILIDEFQDTSKIQYFLVKLLALQN----SDITIVGDPDQSIYGFRSAEIRNLNQMSE--D 288
Query: 299 IEYDQIV-LKENYRSAQAILDISETVIKQQKSGRDNREPLRAQFVTDNKPVYMSFPSDDT 357
E Q++ L+ NYRSA+ IL+++ ++I Q KS ++ L++ ++ KP Y F +++
Sbjct: 289 FEGTQVLHLERNYRSAKPILELALSIISQDKS--RPKKGLKSNHISSLKPHYRLFETNNK 346
Query: 358 EAYTITKEI-LYLKAIPGLFKYKDFAILVRLKRSLRTFENALIAHKIPYVIQKGYTFWEL 416
E+Y I +EI + + P L Y D AILVR R+ E+AL +PY + F++
Sbjct: 347 ESYWIAREIKRIVGSCPELIFYNDIAILVRSSSLTRSLEHALSELGVPYRMVGVNKFFDR 406
Query: 417 KECKAIIRYMSMICNDDDWESLKRTINYPNRGVGETTVGKIAAAFEKESTAEKNETAFSF 476
+E + +I Y+ ++ N D SL R IN P R +G+T + +I FE E + T +
Sbjct: 407 EEIRDLIAYLRVLANKDS-TSLIRVINVPPRNIGKTKIDRI--IFESE---RRGLTFWQT 460
Query: 477 LQKIARDSVKINISNSSMIGIKDYVNIIEQVRKFFSEIYSDAGKSTIADAFEKFYVLTGL 536
L ++ +++ ++ N +K + + + K + S+ +T++D +L+ +
Sbjct: 461 LNEVKNENILLSQRNDKSF-LKSLKSFLCSISKLENRYLSNGHSATLSDLL--LGILSEI 517
Query: 537 RGQYKPEKKSGSIASDDQSTEKEAHRHLNVLNVKNYFSTFKIPLGDGDGEPRIVDLIGFC 596
+ +K+ + E + + ++ Y +KI IV L F
Sbjct: 518 KYYEYLVRKNKETVEEKWENVMELVQQSDNISCIFYELDYKI--------STIVLLQNF- 568
Query: 597 HEFVSFTDLYTFNEDNDNDVKDDHETKDAVVLSTIHASKGLEWPVVFVPQCVEGLLPSYY 656
T + NE+ K+ K V +ST+HA+KGLEWPVVF+P E ++P
Sbjct: 569 -----LTQIALVNEEQ----KEGESQK--VTISTLHAAKGLEWPVVFLPCLCENIIPHSR 617
Query: 657 KDSSDMPAPDEDSDENMAVGDAYNEPTDSQDDNRDGXXXXXXXXXXXXXXFSEERRMFFV 716
D D EERR+ +V
Sbjct: 618 SDDLD-----------------------------------------------EERRLLYV 630
Query: 717 ALTRAKYLLYITTVEKEATSF 737
TRA+ LLY+++ + F
Sbjct: 631 GATRAQALLYLSSFKSVTGMF 651
>YOL095c [L] KOG2108 3'-5' DNA helicase
Length = 706
Score = 86.3 bits (212), Expect = 2e-16
Identities = 143/668 (21%), Positives = 265/668 (39%), Gaps = 100/668 (14%)
Query: 10 LSNLNDAQRKAVIYDYTKA--LQIIAGPGTGKTKVLTARFAYLVLSQKINAEEIIMTTFT 67
+ L +Q K + Y A +++IAGPG+GKT L + +L+ + I EEI++ + T
Sbjct: 1 MDKLTPSQWKVINKSYEPASTIKVIAGPGSGKTLTLLYKVLHLITVENIKPEEILIFSLT 60
Query: 68 RKAAEEMKERLVPVLVSNGISP---HGLKIGTFHSICWKLLKEKGFLIGKEKIERATTEK 124
KA + + E L+ + ++ + H + T H + +++ E +I IE
Sbjct: 61 NKAVDSIIENLLSIFENSHTNKEIVHQIGCYTVHGLANRIVVENEGMI--NIIEEIGWRG 118
Query: 125 IKSILEELIVEMPDQIRDYACTKSYKNEVSLCRLKNNQWQVHPDMILKRISELKADAITP 184
+ +L P R Y + + L KNN + +++++ EL +
Sbjct: 119 LMKLLPP-SKRTPHHFRSYKELEKVVKDYKLNNAKNN------NPVIEKLVELMDNC--- 168
Query: 185 EKYEQQAIFDEALLHFYQQYQAKLSKEHLIDFDDVMLYSFELLSKHRVWKNIKHVLVDEF 244
+ + ++ L+ ++Y S + D ++ +L +K++V VL+DEF
Sbjct: 169 -----KVMTNDDLIIRAKKYLELDSSD-----SDASSFTQDLRNKYKV------VLIDEF 212
Query: 245 QDTNLLQIHLIFQFARGSHHSCEGVTVVGDPDQGIYGFRSALSHNFQTMIELCPIEYDQI 304
QD LI +G + + GD +Q IYGF + + + L P +
Sbjct: 213 QDLYPSLAPLITMICKGKQ-----LIMFGDTNQSIYGFLGSNNEIMSQLDNLHPKNSTTV 267
Query: 305 V-LKENYRSAQAILDISETVIKQ---QKSGRDNREPLRAQFV----TDNKPVYMSFPSDD 356
+ L +N+RS I+ ++ +I + +K D+ + ++ V + P M+F
Sbjct: 268 LKLFDNFRSTPEIISLASKIINRPLAEKQIIDDTDETPSELVRKLPSGVSPQIMTFDDLA 327
Query: 357 TEAYTITKEILYLKAIPGLFKYKDFAILVRLKRSLRTFENALIAHKIPYVIQKGYTFW-- 414
E+ I +I L I K+ D AIL R L + L + IPY K W
Sbjct: 328 AESEFIIDKITQL--ICSSAKFSDIAILSRTNSHLTAIASILKKYGIPYQKLKSQPDWMD 385
Query: 415 --ELKECKAIIRYMSMICND---------DDWESLKRTINYPN--RGVGETTVGKIAAAF 461
++ I++ S+ ++ D W+S + + +G+G+ ++ + A
Sbjct: 386 DLRIQFLLDILKVCSLASDEKHNREFNTGDKWQSNFSILVTMSALKGIGDASIQALYKA- 444
Query: 462 EKESTAEKNETAFSFLQKIARDSVKINISNSSMIGIKDYVNIIEQVRKFFSEIYSDAGKS 521
+ KN + + +L + + +S I +++ + S +Y
Sbjct: 445 ----CSLKNLSIWKYLTMVPNFEWPLGLS------------IKKKMENYTSNLYEMIEND 488
Query: 522 TI---ADAFEKFYVLTGLRGQYKPEKKSGSIASDDQSTEKEAHRHLNVLNVKNYFSTFKI 578
+ D E + + SD QS+ E HL + S
Sbjct: 489 QVHQLDDPMELLEKVASITNNLNLNPTYFQSLSDAQSS-LEFKTHLQEMAQVMKVSKSNK 547
Query: 579 PLGDGDGEPRIVDLIGFCHEFVS--FTDLYTFNEDNDNDVKDDHETKDAVVLSTIHASKG 636
P G I F F+ F F++ T V LSTIH++KG
Sbjct: 548 PPG-----------ISFVKWFLETYFDQTMVFHQSQQALQTTGPGT---VKLSTIHSAKG 593
Query: 637 LEWPVVFV 644
LE+P+VF+
Sbjct: 594 LEFPIVFL 601
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.133 0.379
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,427,207
Number of Sequences: 60738
Number of extensions: 2859344
Number of successful extensions: 9755
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 9729
Number of HSP's gapped (non-prelim): 11
length of query: 1064
length of database: 30,389,216
effective HSP length: 117
effective length of query: 947
effective length of database: 23,282,870
effective search space: 22048877890
effective search space used: 22048877890
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)