ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI3791 good L KOG2108 Replication, recombination and repair 3'-5' DNA helicase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI3791 1321231 1324128 966
(966 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL092w [L] KOG2108 3'-5' DNA helicase 497 e-140
SPAC4H3.05 [L] KOG2108 3'-5' DNA helicase 270 8e-72
YOL095c [L] KOG2108 3'-5' DNA helicase 95 6e-19
>YJL092w [L] KOG2108 3'-5' DNA helicase
Length = 1174
Score = 497 bits (1280), Expect = e-140
Identities = 336/963 (34%), Positives = 519/963 (53%), Gaps = 86/963 (8%)
Query: 4 LTNGLNERQRQAVTHREDDVLQVLAGPGTGKTKVLTARFAYLVIEKNIHPLRIIMTTFTR 63
L + LN +QR A LQV+AGPGTGKTKVLT+R AYL++ +IHP II+TTFT
Sbjct: 11 LVSQLNTQQRAAALFDYTRGLQVIAGPGTGKTKVLTSRVAYLILHHHIHPRDIIVTTFTN 70
Query: 64 KAANEIKERLQPILSQAGISSNGLLIGTFHSICARLLRQAGHLIGIPKNWSIAKTEDINT 123
KAANE+KERLQ +L AG++ + LLIGTFHSIC ++L + GHL+ + K+W I ++I+
Sbjct: 71 KAANEMKERLQEMLRGAGVNISELLIGTFHSICLKILYRFGHLVDLQKDWRIIDEKEIDV 130
Query: 124 ILKTLLEDTPVEIIQ-SSTFTTELS---------ENRTNVRFLRTKISDLKAKGVTPQAY 173
IL ++E P +I +S+ T +++ E + + ++ +IS LK+ + P+ Y
Sbjct: 131 ILDDMIEKVPDQIRDYASSITRKVNLCMPSKNGDEWTIHPKLIKKQISKLKSNAILPEEY 190
Query: 174 LKDQSHDSAIQYIYERFQDTLLSEALLDFDDILLYSKILLMQRRIWSFVRHVLVDEYQDT 233
+ D +HD+A+ Y Y+ +Q L + LDFDD+L+Y+ LL + R+ S ++HVLVDE+QDT
Sbjct: 191 ILDSNHDAALGYFYQIYQSELSKKNTLDFDDLLMYTFRLLTRVRVLSNIKHVLVDEFQDT 250
Query: 234 NELQLDLIYLLARGKHDKCSGITVVGDPDQSIYAFRFASSDNFNKLIERCPVQSAQISLI 293
N +QLDL++L A+G H G+T+VGDPDQSIYAFR A + NF ++ +CP++ + I L+
Sbjct: 251 NGIQLDLMFLFAKGNHHLSRGMTIVGDPDQSIYAFRNALAHNFLEMGRKCPIEYSTIILV 310
Query: 294 DNYRSAQTLLDFSEKVIGQQIKNRIKRDPLRAQFNHNVKPFYSYFQRTDTFENSESNSLA 353
+NYRS+Q +L+ SE +I QQ K R R PLRAQF+ + P Y F E+ SL
Sbjct: 311 ENYRSSQKILNTSEILITQQNKGRQNRAPLRAQFDLDFPPVYMNFPAYFL----EAPSLV 366
Query: 354 HEILYLKSLPGLFKFSDVAILLRTRRQVYDIEKALIKNRIPYRIVNAKGVWEKKESGIFL 413
E+LYLK+LP LF F+D AIL+R RRQ+ IE ALI++RIPY+I+ W+ KE+ L
Sbjct: 367 RELLYLKALPNLFTFNDFAILVRQRRQIKRIESALIEHRIPYKIIRGHSFWDSKETRAML 426
Query: 414 DYLRIISQDSCSLAILRCLQFASNGIGSTTIRKIKSAFDKDRCRNS-LEVLKELCESNDL 472
+ L++I + AIL L + + G+G T KIK+A D S ++LK++ +
Sbjct: 427 NLLKLIFSPNDKHAILASLLYPARGLGPATGEKIKNALDTLATDVSCFQILKDISSKKIM 486
Query: 473 NN--QKISAALKRFINLVEKLKLKCEEYDTYDGLKELFAEIYMLGEL-YSVLKISKETIN 529
+ K + + FI+++E +L + GL +LF ++Y L L Y L + N
Sbjct: 487 LDIPTKGRSVIADFISMIENCQLLLQS-TLLGGLSDLFDKLYELSGLKYEYLYKDGKKKN 545
Query: 530 EQIKDRSLKEFLQTEHQNMFKVYELF------TEYELDQNDEHIMIQENAADPNDLALIK 583
+Q+ ++S L H+N+ + F +E + +E I + A+P + +I
Sbjct: 546 DQL-EKSEPNLLNARHKNIELLKNYFLALLSKSESSDKEKNEAIKAATDEAEPIENKVIT 604
Query: 584 ENFNSNDLNFFEVITFFTKSISLYS--SEEENTENSLD-KLANHLQERVTVSTIHAAKGL 640
E + F S+SL+S +EEE +E++ D K+ VT+STIH AKGL
Sbjct: 605 PK---------EYLRNFFNSLSLHSDAAEEEESESNKDAKIKREKNGFVTISTIHGAKGL 655
Query: 641 EWPVVFIPSINEGLIP--FTEQSARQRLEDLAELRRDGEACNDLNNCSMGSGEFDNIKEE 698
EWPVVFIP EG+IP F + + ED E + +N K++
Sbjct: 656 EWPVVFIPGCEEGIIPCVFNDDKKDESEEDEEE-------------------DQENSKKD 696
Query: 699 LRGKESDLIDDLETTLNEERRIFFVALTRAKDLLYLSSS-----------GKKSIFLKNC 747
K++ ++ +E +++EERR+FFVA TRAK LLYLS++ + +
Sbjct: 697 ASPKKTRVL-SVEDSIDEERRMFFVAQTRAKYLLYLSNTVTVEDVDRPRIASRFLTTDLI 755
Query: 748 EQLYSNNVLFNNPQSILRFYRCMNRTLEETSSK-FSLKSLCQDYQKSKNCKTSTFIWNGT 806
+ + + LF + SI + YR +N+ K FSL L +DY + + IW G
Sbjct: 756 KAMSDSQKLFESTNSIKKLYRILNKKPPAEDDKLFSLDQLRKDYNQFIENRRERMIWQGI 815
Query: 807 NVQTPALIDFNNNTAPSSFSTGTNSFKPASSFLNASFSPGK--------NSYNSGTANVT 858
+ I + N S S T++ S P K + N A +
Sbjct: 816 QMNDVYGIQLSRNKLLGSVSDFTSAADQLRLETQNSIFPQKKLIEKSRPSKINGNYAPKS 875
Query: 859 KVLTTSSKECPK------PAGGKICEKKRRITLDMLCKKPVKNSQSMQSKKIRLESSSAQ 912
+V + + P+ P K+ + T ++ ++ +S +R E S
Sbjct: 876 RVKSPEKRYAPETTSFHSPTKKKVYAPQYVSTTNVPSRQEFHSSTGKNIPFLRREDRSIT 935
Query: 913 DLS 915
D+S
Sbjct: 936 DIS 938
>SPAC4H3.05 [L] KOG2108 3'-5' DNA helicase
Length = 887
Score = 270 bits (690), Expect = 8e-72
Identities = 228/688 (33%), Positives = 350/688 (50%), Gaps = 93/688 (13%)
Query: 8 LNERQRQAVTHREDDVLQVLAGPGTGKTKVLTARFAYLVIEKNIHPLRIIMTTFTRKAAN 67
LNE QR +V Q+LAGPG+GKT+VLTAR AYL+ + +I +I+ TFT KAAN
Sbjct: 11 LNEEQRISV-QSPHKYTQILAGPGSGKTRVLTARVAYLLQKNHIAAEDLIIATFTNKAAN 69
Query: 68 EIKERLQPILSQAGISSNGLLIGTFHSICARLLRQAGHLIGIPKNWSIAKTEDINTILKT 127
EIK R++ IL + S L+ GTFHSI + L + G IG+ NW IA D I+K
Sbjct: 70 EIKLRIEAILGNSEASK--LISGTFHSIAYKYLVKYGKHIGLSSNWLIADRNDTQAIMKR 127
Query: 128 LLEDTPV--EIIQSSTFTTELSENRTNVRFLRTKISDLKAKGVTPQAYLKD--------- 176
LL+ I S EL+ R + K + L K Q L +
Sbjct: 128 LLDSLKKAKNPIASGIRGQELTPQNALNRITKLKSNGLLVKPGMDQLSLINGLEEPPKEL 187
Query: 177 QSHDSAIQYIYERFQDTLLSEALLDFDDILLYSKILLMQRR--IWSFVRHVLVDEYQDTN 234
QSH S +Y +Q +L L DFDD+LL + ILL+Q++ ++H+L+DE+QDT+
Sbjct: 188 QSHQSV--ELYRLYQTSLWKNNLADFDDLLL-NFILLLQKQPDCVRNIKHILIDEFQDTS 244
Query: 235 ELQLDLIYLLARGKHDKCSGITVVGDPDQSIYAFRFASSDNFNKLIERCPVQSAQISLID 294
++Q L+ LLA D IT+VGDPDQSIY FR A N N++ E + Q+ ++
Sbjct: 245 KIQYFLVKLLALQNSD----ITIVGDPDQSIYGFRSAEIRNLNQMSE--DFEGTQVLHLE 298
Query: 295 -NYRSAQTLLDFSEKVIGQQIKNRIKRDPLRAQFNHNVKPFYSYFQRTDTFENSESNSLA 353
NYRSA+ +L+ + +I Q K+R K+ L++ ++KP Y F+ N ES +A
Sbjct: 299 RNYRSAKPILELALSIISQD-KSRPKKG-LKSNHISSLKPHYRLFETN----NKESYWIA 352
Query: 354 HEILYLK-SLPGLFKFSDVAILLRTRRQVYDIEKALIKNRIPYRIVNAKGVWEKKESGIF 412
EI + S P L ++D+AIL+R+ +E AL + +PYR+V ++++E
Sbjct: 353 REIKRIVGSCPELIFYNDIAILVRSSSLTRSLEHALSELGVPYRMVGVNKFFDREEIRDL 412
Query: 413 LDYLRIISQDSCSLAILRCLQFASNGIGSTTIRKIKSAFDKDRCRNSL-EVLKELCESND 471
+ YLR+++ S +++R + IG T I +I F+ +R + + L E+ N
Sbjct: 413 IAYLRVLANKD-STSLIRVINVPPRNIGKTKIDRI--IFESERRGLTFWQTLNEVKNENI 469
Query: 472 L----NNQKISAALKRFINLVEKLKLKCEEYDTYDGLKELFAEIYMLGELYSVL-KISKE 526
L N++ +LK F+ + KL+ + L +L I + Y L + +KE
Sbjct: 470 LLSQRNDKSFLKSLKSFLCSISKLENRYLSNGHSATLSDLLLGILSEIKYYEYLVRKNKE 529
Query: 527 TINEQIKDRSLKEFLQTEHQNMFKVYELFTEYELDQNDEHIMIQENAADPNDLALIKENF 586
T+ E K ++ E +Q + N+ ++ YELD I++ +N
Sbjct: 530 TVEE--KWENVMELVQ-QSDNISCIF-----YELDYKISTIVLLQN-------------- 567
Query: 587 NSNDLNFFEVITFFTKSISLYSSEEENTENSLDKLANHLQERVTVSTIHAAKGLEWPVVF 646
F I+L + E++ E+ ++VT+ST+HAAKGLEWPVVF
Sbjct: 568 -------------FLTQIALVNEEQKEGES----------QKVTISTLHAAKGLEWPVVF 604
Query: 647 IPSINEGLIPFTEQSARQRLEDLAELRR 674
+P + E +IP R +DL E RR
Sbjct: 605 LPCLCENIIP------HSRSDDLDEERR 626
>YOL095c [L] KOG2108 3'-5' DNA helicase
Length = 706
Score = 94.7 bits (234), Expect = 6e-19
Identities = 143/650 (22%), Positives = 275/650 (42%), Gaps = 96/650 (14%)
Query: 24 LQVLAGPGTGKTKVLTARFAYLVIEKNIHPLRIIMTTFTRKAANEIKERLQPILSQAGIS 83
++V+AGPG+GKT L + +L+ +NI P I++ + T KA + I E L I + +
Sbjct: 22 IKVIAGPGSGKTLTLLYKVLHLITVENIKPEEILIFSLTNKAVDSIIENLLSIFENSHTN 81
Query: 84 SNGL-LIG--TFHSICARLLRQAGHLIGIPKNWSIAKTEDINTILKTLLEDTPVEIIQSS 140
+ IG T H + R+ + + E + I++ + ++++ S
Sbjct: 82 KEIVHQIGCYTVHGLANRI---------------VVENEGMINIIEEIGWRGLMKLLPPS 126
Query: 141 TFTTELSENRTNVRFLRTKISDLKAKGVTPQAYLKDQSHDSAIQYIYERFQDTLLSEALL 200
T + + + L + D K ++++ I+ + E + ++
Sbjct: 127 KRTPH---HFRSYKELEKVVKDYKLNNA--------KNNNPVIEKLVELMDNC----KVM 171
Query: 201 DFDDILLYSKILL-----------MQRRIWSFVRHVLVDEYQDTNELQLDLIYLLARGKH 249
DD+++ +K L + + + + VL+DE+QD LI ++ +GK
Sbjct: 172 TNDDLIIRAKKYLELDSSDSDASSFTQDLRNKYKVVLIDEFQDLYPSLAPLITMICKGKQ 231
Query: 250 DKCSGITVVGDPDQSIYAFRFASSDNFNKLIERCPVQSAQI-SLIDNYRSAQTLLDFSEK 308
+ + GD +QSIY F ++++ ++L P S + L DN+RS ++ + K
Sbjct: 232 -----LIMFGDTNQSIYGFLGSNNEIMSQLDNLHPKNSTTVLKLFDNFRSTPEIISLASK 286
Query: 309 VIGQQIKNRIKRDPLRAQFNHNVKPFYSYFQ-RTDTFEN--SESNSLAHEILYLKSLPGL 365
+I + + + D + V+ S + TF++ +ES + +I L +
Sbjct: 287 IINRPLAEKQIIDDTDETPSELVRKLPSGVSPQIMTFDDLAAESEFIIDKITQL--ICSS 344
Query: 366 FKFSDVAILLRTRRQVYDIEKALIKNRIPYRIVNAKGVW-EKKESGIFLDYLRIIS---Q 421
KFSD+AIL RT + I L K IPY+ + ++ W + LD L++ S
Sbjct: 345 AKFSDIAILSRTNSHLTAIASILKKYGIPYQKLKSQPDWMDDLRIQFLLDILKVCSLASD 404
Query: 422 DSCSLAILRCLQFASNGIGSTTIRKIKSAFDKDRCRNSLEVLKELCESNDLNNQKISAAL 481
+ + ++ SN T+ +K D S++ L + C +L+ K +
Sbjct: 405 EKHNREFNTGDKWQSNFSILVTMSALKGIGDA-----SIQALYKACSLKNLSIWKYLTMV 459
Query: 482 KRFI-NLVEKLKLKCEEYDTYDGLKELFA--EIYMLGELYSVL-KISKETINEQIKDRSL 537
F L +K K E Y + L E+ +++ L + +L K++ T N +
Sbjct: 460 PNFEWPLGLSIKKKMENYTS--NLYEMIENDQVHQLDDPMELLEKVASITNNLNLNPTYF 517
Query: 538 KEFLQTEHQNMFKVYELFTEYELDQNDEHIMIQENAADPNDLALIKENFNSNDLNFFEVI 597
+ + FK + Q +M + P ++ +K F E
Sbjct: 518 QSLSDAQSSLEFKTHL--------QEMAQVMKVSKSNKPPGISFVKW--------FLE-- 559
Query: 598 TFFTKSISLYSSEEENTENSLDKLANHLQERVTVSTIHAAKGLEWPVVFI 647
T+F +++ + S++ L V +STIH+AKGLE+P+VF+
Sbjct: 560 TYFDQTMVFHQSQQA--------LQTTGPGTVKLSTIHSAKGLEFPIVFL 601
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.132 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,259,291
Number of Sequences: 60738
Number of extensions: 2404236
Number of successful extensions: 8386
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 8366
Number of HSP's gapped (non-prelim): 7
length of query: 966
length of database: 30,389,216
effective HSP length: 116
effective length of query: 850
effective length of database: 23,343,608
effective search space: 19842066800
effective search space used: 19842066800
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)