ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI3799 suspect: LH L KOG1929 Replication, recombination and repair Nucleotide excision repair factor NEF2, RAD4/CUT5 component
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI3799 1327871 1325865 -669
(669 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL090c [L] KOG1929 Nucleotide excision repair factor NEF2 RAD4/... 360 5e-99
SPAC23C4.18c [L] KOG1929 Nucleotide excision repair factor NEF2 ... 134 6e-31
>YJL090c [L] KOG1929 Nucleotide excision repair factor NEF2 RAD4/CUT5
component
Length = 764
Score = 360 bits (923), Expect = 5e-99
Identities = 255/778 (32%), Positives = 384/778 (48%), Gaps = 130/778 (16%)
Query: 4 KPFQGLVFCPTSLPLSV-SDTISHKVTKLGGTYTKDLTRLVNVLVVGELF-TAKYNFAVK 61
KPFQG+ FCPT++ + + IS K+ KLGG ++KDLTR VNVLVVG T K+ FAVK
Sbjct: 2 KPFQGITFCPTAINNEILAKKISKKIIKLGGIFSKDLTRQVNVLVVGSTTNTNKFKFAVK 61
Query: 62 NRTDIVFVSPGSVDQLYDAWLAGEDLLQLAK--------FDMSQYLKSKYSIGSFEKFNL 113
+R DI+F+ ++D +Y WL+GE++L + ++M + L ++S FN+
Sbjct: 62 HRFDIIFIDIQAIDDIYQLWLSGENILPDSNTATMTGSTYEMLKILYRRFSFKYLHNFNI 121
Query: 114 FIGRIRPNNHYSLESLVQLCKLGKVKSVDTTHFIRNSSSS---------NACISLFITDD 164
FIGRI N S++SLV+ K S + +F+ +SS N IS+F+TD
Sbjct: 122 FIGRITDTNITSIDSLVRSIKKLGCSSYNYQNFVIKDTSSHNDDDDQGQNGQISIFVTDT 181
Query: 165 LSGNRVQAALEENVPVVHPKWIIDCMDRSGTLDFQSFYLLKNCHELEYSDIGSGS----- 219
L G RV AA+E+N+P+VH KWI+DC RS L + +YLL N +L Y IGS S
Sbjct: 182 LLGARVNAAIEQNIPIVHFKWILDCQKRSALLPYDPYYLLPNIKDLPYDSIGSNSCDCWD 241
Query: 220 -----YIRDVPDKKKSVTEPNPMGVQDDSVNPLKIAIDKFKPQANQIWGKLLARDGDASA 274
+ ++ D + S+ S+ ++KFKP+ +IW K ++ +
Sbjct: 242 KINTTFPTNI-DAQSSLQRQQSSSTLTPSLPKTSSLLNKFKPKGEKIWDKAMSLQQHSKT 300
Query: 275 SKST--DIKEATPTHQECLGETKDTPTFLDCHFVLFK-FEKRQVTILSNVIRGNKG---T 328
+ S + QE L + T F +C F++ F +IL+ ++ N G T
Sbjct: 301 NFSVLGQSPLSINNKQEDLSD-NSTLIFKNCAFIIHHIFPGNHRSILTKIVVQNGGKIET 359
Query: 329 SSLFEDTSPSPAQLKDKTIYYICPSGFPA---NLLPRIPNESSWL-TEFFIERCLYYEQL 384
S L + + D + YYI PS N LP I +++ + TEFFIERCLYY++L
Sbjct: 360 SYL--------SGIYDHS-YYIIPSNKALDSFNDLPEIIDDNDGIVTEFFIERCLYYQKL 410
Query: 385 LPP-DFWSKPFYN--DFNIIPPKSVLH-----GPKLSFHITGFHGVELLHINKIIDFLKL 436
L P D WSKPF + +F + +LH P L+ ITGF GVELLH+ K+++ LK
Sbjct: 411 LHPIDLWSKPFLSTIEFQVSSSSKLLHHEFSSSPFLNVTITGFSGVELLHLTKVLNLLKP 470
Query: 437 IGFDHHEKLTKKTDFLLVNISQLTSIPQEHTLRQNKYASMFRT-----------DLDMKI 485
+G ++ E L K TD LL+N++ L SIP+ H L N+++ +F D + K
Sbjct: 471 MGINYVEYLNKSTDILLINLAALPSIPKTHPLWSNEFSDLFTQFCINNNNDDPGDNNRKD 530
Query: 486 SVNQAQMFRNSMKRKIEFVKQAHHIPILTPSFVIELFQRAMKPNKTSMNIYFNDVNWCIS 545
N + + RNSMKRKIE++K+ H IP++TP+F+ +L A N I+ N++ WCI
Sbjct: 531 FQNNS-ILRNSMKRKIEYIKKFHSIPVVTPAFIFKLLSAASGENN---EIFLNNIKWCII 586
Query: 546 CPKGSKSDFALQI---------IPKERGNEDPLLEK------------------------ 572
CP+G K DF +I K+ N DP ++K
Sbjct: 587 CPRGHKDDFKCKIKKPYYTSISSEKKYQNNDPKIDKTILLKRNNSSLSEHSMKDTKNELL 646
Query: 573 ----------------PAPVDIXXXXXXXXXXXXELPHVP--------NRGNRWGKLLSN 608
+ +D+ +L +P R WG ++S
Sbjct: 647 QKIRETDSGRKKRSVSSSIMDVSSERQMPDTKRIKLESLPKNFVPKQIKRTTSWGTIMSE 706
Query: 609 NVDTENEESSFIQTVGHDSDHLSHTQITYGSASTSTSVQQNLKRRKISTRSQAKDIIS 666
NV TE + ++ +SHTQ+TYGS K + TR+Q K++ S
Sbjct: 707 NVPTEQPTAISNPEEIPRTEEVSHTQVTYGSIQDKKRTASLEKPMRRQTRNQTKELDS 764
>SPAC23C4.18c [L] KOG1929 Nucleotide excision repair factor NEF2 RAD4/CUT5
component
Length = 648
Score = 134 bits (336), Expect = 6e-31
Identities = 133/488 (27%), Positives = 207/488 (42%), Gaps = 78/488 (15%)
Query: 2 SGKPFQGLVFCPTSLPLSVSDTISHKVTKLGGTYTKDLTRLVNVLVVGELFTAKYNFAVK 61
S KP +G V C TS+ L IS K TKLG Y D T+ V L+ G+ T KY FA K
Sbjct: 3 SSKPLKGFVICCTSIDLKQRTEISTKATKLGAAYRSDFTKDVTHLIAGDFDTPKYKFAAK 62
Query: 62 NRTDIVFVSPGSVDQLYDAWLAGEDLLQLAKFDMSQYLKSKYSIGSFEKFNLFIGRIRPN 121
+R DI +S + LY++W+ GEDL L K+ + + K + + I
Sbjct: 63 SRPDIKIMSSEWIPVLYESWVQGEDL-------DDGLLVDKHFLPTLFKCRVCLTNIGQP 115
Query: 122 NHYSLESLVQLCKLGKVKSVDTTHFIRNSSSSNACISLFITDDLSGNRVQAALEENVPVV 181
+E+ V K G D T + + I SG + + AL+ + VV
Sbjct: 116 ERSRIENYV--LKHGGTFCPDLTRDVTH----------LIAGTSSGRKYEYALKWKINVV 163
Query: 182 HPKWIIDCMDRSGTLDFQSFYLLKNCHELEYSDIGSGSYIRDVPD--KKKSVTEPNPMG- 238
+W+ + R+ L+ Q F L ++ IG G+Y+R P+ + KS +E +
Sbjct: 164 CVEWLWQSIQRNAVLEPQYFQL-----DMPAEKIGLGAYVRLDPNTTEAKSYSENQKISK 218
Query: 239 ------------VQDDSVNPLKIAIDKFKPQANQIWGKL---LARDGDASASKSTDIKEA 283
++ + P+ + K K + +W +L S S + +
Sbjct: 219 NKEKSGQSLAALAEEADLEPVIMKRGK-KRDRSILWEELNNGKFEFSSRSEENSVLLDDF 277
Query: 284 TPTHQECLGETK---------DTPTFLDCHFVLFKFEKRQVTILSNVIRGNKGTSSLFED 334
TP + L E + + F + F L++F +V+ L + N G S F
Sbjct: 278 TPETVQPLEENELDTELNIENEAKLFKNLTFYLYEFPNTKVSRLHKCLSDNGGQISEF-- 335
Query: 335 TSPSPAQLKDKTIYYICPSGFPANLLP--RIPNESSWLTEFFIERCLYYEQLLPPD--FW 390
L + + P FP + LP P + W +IERCLYY+++ D
Sbjct: 336 -------LSSTIDFVVIPHYFPVDELPIFSFPTVNEW----WIERCLYYKKIFGIDEHAL 384
Query: 391 SKPFYNDFNIIPPKSVLHGPKLSFHITGFHGVELLHINKIIDFLKLIGFDHHEKLTKKTD 450
+KPF+ P V + LS H+TGF G EL H+ K L ++G HE L +
Sbjct: 385 AKPFFR------PSLVPYFNGLSIHLTGFKGEELSHLKKA---LTILGAVVHEFLGVQRS 435
Query: 451 FLLVNISQ 458
LLVN ++
Sbjct: 436 ILLVNTNE 443
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.136 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 41,611,196
Number of Sequences: 60738
Number of extensions: 1871436
Number of successful extensions: 4240
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 4224
Number of HSP's gapped (non-prelim): 3
length of query: 669
length of database: 30,389,216
effective HSP length: 113
effective length of query: 556
effective length of database: 23,525,822
effective search space: 13080357032
effective search space used: 13080357032
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)