ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI3984 good U KOG3103 Intracellular trafficking, secretion, and vesicular transport Rab GTPase interacting factor, Golgi membrane protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI3984 1389925 1390725 267
(267 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR172c [U] KOG3103 Rab GTPase interacting factor Golgi membrane... 233 3e-61
SPCC61.04c [U] KOG3103 Rab GTPase interacting factor Golgi membr... 145 6e-35
Hs21314734 [U] KOG3103 Rab GTPase interacting factor Golgi membr... 118 8e-27
7297993 [U] KOG3103 Rab GTPase interacting factor Golgi membrane... 113 3e-25
HsM13540584 [U] KOG3103 Rab GTPase interacting factor Golgi memb... 110 2e-24
CE23698_1 [U] KOG3103 Rab GTPase interacting factor Golgi membra... 106 3e-23
ECU03g0590 [U] KOG3103 Rab GTPase interacting factor Golgi membr... 79 9e-15
At3g52760 [U] KOG3103 Rab GTPase interacting factor Golgi membra... 71 1e-12
At2g36300 [U] KOG3103 Rab GTPase interacting factor Golgi membra... 69 5e-12
Hs14150076 [U] KOG3103 Rab GTPase interacting factor Golgi membr... 50 4e-06
>YGR172c [U] KOG3103 Rab GTPase interacting factor Golgi membrane protein
Length = 248
Score = 233 bits (593), Expect = 3e-61
Identities = 129/256 (50%), Positives = 158/256 (61%), Gaps = 21/256 (8%)
Query: 15 SNTNGFYQPSQQYSFPQGSMAFGNGMQGSGMDFGGSKPSGTPANF--TNEQLTPGLFNAL 72
+N GFYQPS Q++ PQGSM+F N + GS +G N + L G+ +AL
Sbjct: 11 NNGGGFYQPSAQFAVPQGSMSFQNTV--------GSSNTGNDNNLGVAPDPLPVGILHAL 62
Query: 73 STRGYSFEPSLLEELGINFNRIIAKTKFVLVPLSH-KGLSKEIQEDSDLAGPIIFWLMFG 131
ST+GY EP LLEE+GINF+ II KTK VL+P+ G+ +EI DSDLAGP+IF+L+FG
Sbjct: 63 STKGYPHEPPLLEEIGINFDHIITKTKMVLIPIRFGSGVPQEILNDSDLAGPLIFFLLFG 122
Query: 132 ILLLTAGKVHFGYIYGVALFGSLSLHTLLKYMSDGAHQLXXXXXXXXXXXXXXXYLRSAS 191
+ LL AGKVHFGYIYGVALFG++SLH L K MS+ + +AS
Sbjct: 123 LFLLMAGKVHFGYIYGVALFGTISLHNLSKLMSNN----------DTSTQTNLQFFNTAS 172
Query: 192 ILGYAFLPLCVLASLGVFANLNNTFGYIXXXXXXXXXXXXXXXXFTVSLDLHNVRILIAY 251
ILGY FLPLC L+ LG+F LNNT GY+ L L N R+LIAY
Sbjct: 173 ILGYCFLPLCFLSLLGIFHGLNNTTGYVVSVLFVIWSTWTSSGFLNSLLQLQNARLLIAY 232
Query: 252 PLLIFYSVFALMAIFV 267
PLLIFYSVFALM IFV
Sbjct: 233 PLLIFYSVFALMVIFV 248
>SPCC61.04c [U] KOG3103 Rab GTPase interacting factor Golgi membrane protein
Length = 227
Score = 145 bits (366), Expect = 6e-35
Identities = 83/206 (40%), Positives = 109/206 (52%), Gaps = 18/206 (8%)
Query: 61 NEQLTPGLFNALSTRGYSFEPSLLEELGINFNRIIAKTKFVLVPLSHKGLSKEIQEDSDL 120
+E L+ G A ST GY EPSLLEEL INF I KT VL P H + I +D+D+
Sbjct: 38 HEPLSQGWLAAFSTSGYPGEPSLLEELEINFGHIKQKTTHVLNPFKH--VDVHIMDDTDM 95
Query: 121 AGPIIFWLMFGILLLTAGKVHFGYIYGVALFGSLSLHTLLKYMSDGAHQLXXXXXXXXXX 180
AGPI+F L+F L G+ HFGYIYG+AL GSLSLH +L+ MS
Sbjct: 96 AGPILFCLLFSTFLSLHGRSHFGYIYGIALLGSLSLHFVLRLMS---------------- 139
Query: 181 XXXXXYLRSASILGYAFLPLCVLASLGVFANLNNTFGYIXXXXXXXXXXXXXXXXFTVSL 240
+ R+ S+LGY+ LPL V+A N GY F L
Sbjct: 140 AKNLFFTRTVSVLGYSLLPLVVIAFFKNIFTFNGIAGYALAALACIWCTYAASAMFVGIL 199
Query: 241 DLHNVRILIAYPLLIFYSVFALMAIF 266
++N+R L+AYP+ +FY VFA++ +F
Sbjct: 200 QVNNMRFLVAYPIALFYGVFAVITVF 225
>Hs21314734 [U] KOG3103 Rab GTPase interacting factor Golgi membrane protein
Length = 257
Score = 118 bits (296), Expect = 8e-27
Identities = 87/271 (32%), Positives = 118/271 (43%), Gaps = 37/271 (13%)
Query: 14 NSNTNGFYQPS------QQYSFPQGSMAFGNGMQGSGMDFGGSKPSGTPANFTNEQ---- 63
N NT+ FYQ S Q S+ G Q +G D+ P +Q
Sbjct: 6 NLNTD-FYQTSYSIDDQSQQSYDYGGSGGPYSKQYAGYDYSQQGRFVPPDMMQPQQPYTG 64
Query: 64 --------LTPGLFNALSTRGYSFEPSLLEELGINFNRIIAKTKFVLVPLSHKGLSKEIQ 115
TP + EP LLEELGINF+ I KT VL PL K I
Sbjct: 65 QIYQPTQAYTPASPQPFYGNNFEDEPPLLEELGINFDHIWQKTLTVLHPL--KVADGSIM 122
Query: 116 EDSDLAGPIIFWLMFGILLLTAGKVHFGYIYGVALFGSLSLHTLLKYMSDGAHQLXXXXX 175
++DLAGP++F L FG LL AGK+ FGY+YG++ G L + LL MS
Sbjct: 123 NETDLAGPMVFCLAFGATLLLAGKIQFGYVYGISAIGCLGMFCLLNLMS----------- 171
Query: 176 XXXXXXXXXXYLRSASILGYAFLPLCVLASLGVFANLNNTFGYIXXXXXXXXXXXXXXXX 235
+ AS+LGY LP+ +L+S V +L G I
Sbjct: 172 -----MTGVSFGCVASVLGYCLLPMILLSSFAVIFSLQGMVGIILTAGIIGWCSFSASKI 226
Query: 236 FTVSLDLHNVRILIAYPLLIFYSVFALMAIF 266
F +L + ++L+AYP + Y VFAL+++F
Sbjct: 227 FISALAMEGQQLLVAYPCALLYGVFALISVF 257
>7297993 [U] KOG3103 Rab GTPase interacting factor Golgi membrane protein
Length = 264
Score = 113 bits (282), Expect = 3e-25
Identities = 65/187 (34%), Positives = 93/187 (48%), Gaps = 17/187 (9%)
Query: 80 EPSLLEELGINFNRIIAKTKFVLVPLSHKGLSKEIQEDSDLAGPIIFWLMFGILLLTAGK 139
EP LLEELGIN N I KT VL PL +G ++I +D+D+AGP++F L G LL +GK
Sbjct: 95 EPPLLEELGINPNHIFQKTLAVLNPL--RGTDQQILQDTDMAGPLVFCLTLGGFLLLSGK 152
Query: 140 VHFGYIYGVALFGSLSLHTLLKYMSDGAHQLXXXXXXXXXXXXXXXYLRSASILGYAFLP 199
V F YIYG+ + G + + LL M + AS+LGY LP
Sbjct: 153 VTFSYIYGIGVMGCIFFYCLLSLM---------------VSRSQVTFGAVASVLGYCLLP 197
Query: 200 LCVLASLGVFANLNNTFGYIXXXXXXXXXXXXXXXXFTVSLDLHNVRILIAYPLLIFYSV 259
+ VL+ + + + T G I F + + + ++LIAYP + Y
Sbjct: 198 MVVLSGINILITIQGTLGLIVSGISIFWCAISASKLFATAFSMDHQQLLIAYPCAVLYGG 257
Query: 260 FALMAIF 266
FAL+ I+
Sbjct: 258 FALITIY 264
>HsM13540584 [U] KOG3103 Rab GTPase interacting factor Golgi membrane protein
Length = 257
Score = 110 bits (276), Expect = 2e-24
Identities = 85/271 (31%), Positives = 115/271 (42%), Gaps = 37/271 (13%)
Query: 14 NSNTNGFYQPS------QQYSFPQGSMAFGNGMQGSGMDFGGSKPSGTPANFTNEQ---- 63
N NT+ FYQ S Q S+ G Q +G D+ P +Q
Sbjct: 6 NLNTD-FYQTSYSIDDQSQQSYDYGGSGGPYSKQYAGYDYSQQGRFVPPDMMQPQQPYTG 64
Query: 64 --------LTPGLFNALSTRGYSFEPSLLEELGINFNRIIAKTKFVLVPLSHKGLSKEIQ 115
TP + EP LLEELGINF+ I KT VL PL K I
Sbjct: 65 QIYQPTQAYTPASPQPFYGNNFEDEPPLLEELGINFDHIWQKTLTVLHPL--KVADGSIM 122
Query: 116 EDSDLAGPIIFWLMFGILLLTAGKVHFGYIYGVALFGSLSLHTLLKYMSDGAHQLXXXXX 175
++DLAGP++F L LL AGK+ FGY+YG + G L + LL MS
Sbjct: 123 NETDLAGPMVFCLDLAATLLLAGKIQFGYVYGSSAIGCLGMFCLLNLMS----------- 171
Query: 176 XXXXXXXXXXYLRSASILGYAFLPLCVLASLGVFANLNNTFGYIXXXXXXXXXXXXXXXX 235
+ AS+LGY LP+ +L+S V +L G I
Sbjct: 172 -----MTGVSFGCVASVLGYCLLPMYLLSSFAVIFSLQGMVGIILTAGIIGWCSFSASKI 226
Query: 236 FTVSLDLHNVRILIAYPLLIFYSVFALMAIF 266
F +L + ++L+AYP + Y VFAL+++F
Sbjct: 227 FISALAMEGQQLLVAYPCALLYGVFALISVF 257
>CE23698_1 [U] KOG3103 Rab GTPase interacting factor Golgi membrane protein
Length = 244
Score = 106 bits (265), Expect = 3e-23
Identities = 77/247 (31%), Positives = 110/247 (44%), Gaps = 35/247 (14%)
Query: 3 YHQPSIPSIDANSNTNGFYQPSQQYSFPQGSMAFGNGMQGSGMDFGGSKPSGTPANFTNE 62
Y QP P A N +YQ +QQY PQ + +GG P N T
Sbjct: 33 YSQPQQPPQQAG---NDYYQQNQQYQQPQTNY------------YGGQM--FMPNNGTGA 75
Query: 63 QLTPGLFNALSTRGYSFEPSLLEELGINFNRIIAKTKFVLVPLSHKGLSKEIQEDSDLAG 122
G+ A Y EP LLEELGINF+ I KT VL P + E+ D DLAG
Sbjct: 76 IPNAGV--AADGEDYENEPPLLEELGINFSHIKEKTIAVLNPTG--SATVEVIADQDLAG 131
Query: 123 PIIFWLMFGILLLTAGKVHFGYIYGVALFGSLSLHTLLKYMSDGAHQLXXXXXXXXXXXX 182
P++F L+FG LL GK+ FG+IYGV G + ++ L+ M+ +
Sbjct: 132 PLVFCLLFGAALLLHGKLSFGFIYGVGGLGCVGIYALMNLMATDEKNI------------ 179
Query: 183 XXXYLRSASILGYAFLPLCVLASLGVFANLNNTFGYIXXXXXXXXXXXXXXXXFTVSLDL 242
+ +AS+LGY LP+ +L+ + + GYI F ++L +
Sbjct: 180 --SFTCTASVLGYCLLPMALLSIVTAVLSFKGIIGYIISSLAVLWCSSASSKLFVIALSM 237
Query: 243 HNVRILI 249
+ R+L+
Sbjct: 238 DHQRLLM 244
>ECU03g0590 [U] KOG3103 Rab GTPase interacting factor Golgi membrane protein
Length = 198
Score = 78.6 bits (192), Expect = 9e-15
Identities = 49/185 (26%), Positives = 85/185 (45%), Gaps = 17/185 (9%)
Query: 83 LLEELGINFNRIIAKTKFVLVPLSHKGLSKEIQEDSDLAGPIIFWLMFGILLLTAGKVHF 142
LLEELGI+F I ++ K + +++DL+GPI+F ++ + L+ K+HF
Sbjct: 30 LLEELGIDFGTIKKESHLAFGVFQRKAVDFSFVKNADLSGPIVFVGLYTLGLILNYKIHF 89
Query: 143 GYIYGVALFGSLSLHTLLKYMSDGAHQLXXXXXXXXXXXXXXXYLRSASILGYAFLPLCV 202
GYIY ++L +LS++ LL + +L S+LGY+FLP+
Sbjct: 90 GYIYFISLLTTLSIYFLLNVLG----------------TKHIGFLECCSVLGYSFLPVVF 133
Query: 203 LASLGV-FANLNNTFGYIXXXXXXXXXXXXXXXXFTVSLDLHNVRILIAYPLLIFYSVFA 261
+ L + ++ F L L N +++ YPLL+ Y+ F
Sbjct: 134 FSFLNILMGRISVELRVFYGLAFACWSSYAASVVFCRYLSLSNKHLVVGYPLLLGYTGFV 193
Query: 262 LMAIF 266
++ +F
Sbjct: 194 MVILF 198
>At3g52760 [U] KOG3103 Rab GTPase interacting factor Golgi membrane protein
Length = 257
Score = 71.2 bits (173), Expect = 1e-12
Identities = 63/263 (23%), Positives = 110/263 (40%), Gaps = 20/263 (7%)
Query: 6 PSIPSIDANSNTNGFYQPSQQYSFPQGSMAFGNGMQGSGMDFGGSKPSGTPANFTNEQLT 65
P+ S A +N P+ Y P+ S A + + + G + S F +
Sbjct: 13 PTSGSSPATANVQQRRVPTAPYQPPRQSSA-SSSIPFIPFEIGSAAVSMPAGPFDGTIAS 71
Query: 66 PGLFNALSTRGYSFEPSLLEELGINFNRIIAKTKFVLVPLSHKGLSKEIQEDSDLAGPII 125
F + + E LL+ELGI+ ++I KT+ +L P +++ + +DSDL+GPI
Sbjct: 72 SASFGRGVSASFEDEEPLLDELGIHPDQIWKKTRSILNPFR---INQTVHKDSDLSGPIF 128
Query: 126 FWLMFGILLLTAGKVHFGYIYGVALFGSLSLHTLLKYMSDGAHQLXXXXXXXXXXXXXXX 185
+L + L AGK+ FG I G + S+ L+ + ++ L
Sbjct: 129 LYLALCLFQLLAGKIQFGVILGWVVVSSIFLYIVFNMLAGRNGNLNLHT----------- 177
Query: 186 YLRSASILGYAFLPLCVLASLGVFANLNNTFGYIXXXXXXXXXXXXXXXXFTVSL--DLH 243
S++GY+ LP+ +L+++ +F VSL
Sbjct: 178 ---CTSLVGYSLLPVVILSAVSLFVPQGAGPVRFVLGAAFVLWATRACSNLVVSLADGGE 234
Query: 244 NVRILIAYPLLIFYSVFALMAIF 266
R LI+Y + Y++F+L+ IF
Sbjct: 235 EHRGLISYACFLIYTLFSLLVIF 257
>At2g36300 [U] KOG3103 Rab GTPase interacting factor Golgi membrane protein
Length = 283
Score = 69.3 bits (168), Expect = 5e-12
Identities = 56/218 (25%), Positives = 96/218 (43%), Gaps = 23/218 (10%)
Query: 53 SGTPANFTNEQLTPGLFNALSTRGYSFEPSLLEELGINFNRIIAKTKFVLVPLSHKGLSK 112
S TPA + + + E LL+ELGI+ ++I KT+ +L P +++
Sbjct: 85 SATPAGPFGGTIASSSSFGGGSASFEDEEPLLDELGIHPDQIWKKTRSILNPFR---INQ 141
Query: 113 EIQEDSDLAGPIIFWLMFGILLLTAGKVHFGYIYGVALFGSLSLHTLLKYMSDGAHQLXX 172
+ +DSDL+GPI +L + L AGK+ FG I G + S+ L+ + ++ L
Sbjct: 142 AVHKDSDLSGPIFLYLALCLFQLLAGKIQFGVILGWIVVSSIFLYVVFNMLAGRNGNLNL 201
Query: 173 XXXXXXXXXXXXXYLRSASILGYAFLPLCVLASLGVFANLNNTFGYIXXXXXXXXXXXXX 232
S++GY LP+ VL+++ +F + G +
Sbjct: 202 HT--------------CTSLVGYCLLPVVVLSAVSLF--VPQGAGPVRFVLAALFVLWST 245
Query: 233 XXXFTVSLDL----HNVRILIAYPLLIFYSVFALMAIF 266
T+ + L R LIAY + Y++F+L+ IF
Sbjct: 246 RACSTLVVSLADGGEEHRGLIAYACFLIYTLFSLLVIF 283
>Hs14150076 [U] KOG3103 Rab GTPase interacting factor Golgi membrane protein
Length = 244
Score = 49.7 bits (117), Expect = 4e-06
Identities = 44/186 (23%), Positives = 74/186 (39%), Gaps = 33/186 (17%)
Query: 83 LLEELGINFNRIIAKTKFVLVPLSHKGLSKEIQEDS-DLAGPIIFWLMFGILLLTAGKVH 141
LLEEL I+ I K + VL+P+ G ++++ D+ D GP+ L F ++ L
Sbjct: 77 LLEELDIDLKDIYYKIRCVLMPMPSLGFNRQVVRDNPDFWGPLAVVLFFSMISLYGQFRV 136
Query: 142 FGYIYGVALFGSLSLHTLLKYMSDGAHQLXXXXXXXXXXXXXXXYLRSASILGYAFLPLC 201
+I + +FGSL++ L + + Y + ++GY+ LPL
Sbjct: 137 VSWIITIWIFGSLTIFLLARVLG-----------------GEVAYGQVLGVIGYSLLPLI 179
Query: 202 VLA-------SLGVFANLNNTFGYIXXXXXXXXXXXXXXXXFTVSLDLHNVRILIAYPLL 254
V+A S V + L FG V + + L+ YP+
Sbjct: 180 VIAPVLLVVGSFEVVSTLIKLFGVF--------WAAYSAASLLVGEEFKTKKPLLIYPIF 231
Query: 255 IFYSVF 260
+ Y F
Sbjct: 232 LLYIYF 237
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.324 0.141 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,572,955
Number of Sequences: 60738
Number of extensions: 611210
Number of successful extensions: 1455
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1421
Number of HSP's gapped (non-prelim): 10
length of query: 267
length of database: 30,389,216
effective HSP length: 104
effective length of query: 163
effective length of database: 24,072,464
effective search space: 3923811632
effective search space used: 3923811632
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)