ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI4076 good K KOG2393 Transcription Transcription initiation factor IIF, large subunit (RAP74)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI4076 1424591 1426648 686
(686 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR186w [K] KOG2393 Transcription initiation factor IIF large su... 565 e-161
SPCC1620.09c [K] KOG2393 Transcription initiation factor IIF lar... 163 9e-40
At4g12610 [K] KOG2393 Transcription initiation factor IIF large ... 64 8e-10
>YGR186w [K] KOG2393 Transcription initiation factor IIF large subunit
(RAP74)
Length = 735
Score = 565 bits (1457), Expect = e-161
Identities = 352/745 (47%), Positives = 433/745 (57%), Gaps = 82/745 (11%)
Query: 3 NATKSNDASPFIKRDQMRREFLRARQR---SNNPKP-----------IVKKEDPEQAAEE 48
N +ASPFIKRD+MRR FLR R SN+ P +VKK+DPE A E
Sbjct: 11 NGGGPTNASPFIKRDRMRRNFLRMRMGQNGSNSSSPGVPNGDNSRGSLVKKDDPEYAEER 70
Query: 49 ARKLLQ----PEALSRRVK-REPGKSGYSEFPLRAIDMRDKENVKTNLVKFQSKKPIDP- 102
+ LLQ +A VK ++ + Y+EFPLRAI D EN++T+L+KFQSKK I+P
Sbjct: 71 EKMLLQIGVEADAGRSNVKVKDEDPNEYNEFPLRAIPKEDLENMRTHLLKFQSKKKINPV 130
Query: 103 EDFHLPIRLHRKDTRNLQFQLTRAEIVQRQKEIAEFKQRQLEEXXXXXXXXXXXXXXXXX 162
DFHLP+RLHRKDTRNLQFQLTRAEIVQRQKEI+E+K++ +E
Sbjct: 131 TDFHLPVRLHRKDTRNLQFQLTRAEIVQRQKEISEYKKKAEQERSTPNSG---------- 180
Query: 163 XKGNSTGGSFSQSNGKPYNSKQGTPVP--------GTLNGPKVGVTXXXXXXXXXXXXXX 214
G + G+ S +N K G+ P T NG +
Sbjct: 181 --GMNKSGTVSLNN----TVKDGSQTPTVDSVTKDNTANGVNSSIPTVTGSSVPPASPTT 234
Query: 215 XXXXELKTGTDSGSPP--NGKEGTPSNT--------VTILEEAGVAEDPTKVGMVKYDGK 264
E G +GS NG +G S VT LE+AG AEDPTKVGMVKYDGK
Sbjct: 235 VSAVE-SNGLSNGSTSAANGLDGNASTANLANGRPLVTKLEDAGPAEDPTKVGMVKYDGK 293
Query: 265 A---EPEIHEEGTIDPMADVAPHGGGRFRRGNSKRKTRQLKRLDEEAKRLRFEEFYPWVM 321
EPE EEGT+DP+ADVAP GGGR +RGN +RKTRQLK LDE AK+LRFEEFYPWVM
Sbjct: 294 EVTNEPEF-EEGTMDPLADVAPDGGGRAKRGNLRRKTRQLKVLDENAKKLRFEEFYPWVM 352
Query: 322 EDFDGFNTWVGSYEAGNSDSYVLLSVEDDGSFTMIPADKVYRFTARNKYNTLTIEEAEKR 381
EDFDG+NTWVGSYEAGNSDSYVLLSVEDDGSFTMIPADKVY+FTARNKY TLTI+EAEKR
Sbjct: 353 EDFDGYNTWVGSYEAGNSDSYVLLSVEDDGSFTMIPADKVYKFTARNKYATLTIDEAEKR 412
Query: 382 MEKNKEGAPRWFMKHLDESSKXXXXXXXXXXXXXAVDGNTRQAEDDEDRGDNSEVXXXXX 441
M+K PRW MKHLD AV +QA D++DR DNSEV
Sbjct: 413 MDKKSGEVPRWLMKHLDNIGTTTTRYDRTRRKLKAV--ADQQAMDEDDRDDNSEVELDYD 470
Query: 442 XXXXXXXXXXXXXGNEEENKESEQRIKKEMLQANALGLRDDD--GEESDDELFGEKKIDE 499
GNE+ENKESEQRIKKEMLQANA+GLRD++ E +DELFGEKKIDE
Sbjct: 471 EEFADDEEAPIIDGNEQENKESEQRIKKEMLQANAMGLRDEEAPSENEEDELFGEKKIDE 530
Query: 500 EGEKVKKALLKTDLGALYESDAEE-NPYLSHXXXXXXXXXX-XXXXXXXXXXXXXRRSSP 557
+GE++KKAL KT+L ALY SD E NPYLS +RSSP
Sbjct: 531 DGERIKKALQKTELAALYSSDENEINPYLSESDIENKENESPVKKEEDSDTLSKSKRSSP 590
Query: 558 KKKASDTPSG----PPKLVVKSINQNIVVLTGEKSVLSNFPAGAWNPNLKKR-------- 605
KK+ + P L VKSI +++L G+K +L +FP G WNP K
Sbjct: 591 KKQQKKATNAHVHKEPTLRVKSIKNCVIILKGDKKILKSFPEGEWNPQTTKAVDSSNNAS 650
Query: 606 ---PAESQPQESMDNKRAKSDHSADDLLTEDDIINAIEGKQLTLKQLIRQLKEKVGRHPD 662
P+ + +E +++ A+ + + +TE DII AI ++ +K+ + ++ K +
Sbjct: 651 NTVPSPIKQEEGLNSTVAEREETPAPTITEKDIIEAIGDGKVNIKEFGKFIRRKY-PGAE 709
Query: 663 NKERMKVFVKKLV-KLNGKFLELSK 686
NK+ M VKKL K+ +EL K
Sbjct: 710 NKKLMFAIVKKLCRKVGNDHMELKK 734
>SPCC1620.09c [K] KOG2393 Transcription initiation factor IIF large subunit
(RAP74)
Length = 490
Score = 163 bits (412), Expect = 9e-40
Identities = 96/253 (37%), Positives = 140/253 (54%), Gaps = 10/253 (3%)
Query: 278 MADVAPHGGGRFRRGNS-KRKTRQLKRLDEEAKRLRFEEFYPWVMEDFDGFNTWVGSYEA 336
+A +AP+GG + + N+ KRKTRQ+ ++D +A+RL+ EE PW+MEDF+G NTWV + E
Sbjct: 109 IASIAPYGGAQNMKQNAFKRKTRQVVKVDPQARRLQEEELSPWIMEDFEGKNTWVSTMEG 168
Query: 337 GNSDSYVLLSVEDDGSFTMIPADKVYRFTARNKYNTLTIEEAEKRMEKNKEGAPRWFMKH 396
G S +YVL ++G F +IP D+ YRF RN + TL+I+EAE +M K K PRWFMK
Sbjct: 169 GQSSAYVLFMFSENG-FKVIPTDRFYRFNQRNNFQTLSIDEAEAKMNK-KTPIPRWFMKK 226
Query: 397 LDESSKXXXXXXXXXXXXXAVDGNTRQAEDDEDRGDNSEVXXXXXXXXXXXXXXXXXXGN 456
E + V N R G + E+ GN
Sbjct: 227 ESEENLAEAGSASPMYKLKTVP-NARPVTGPRASGSDDEL--DYDEEFADDEEAPIMEGN 283
Query: 457 EEENKESEQRIKKEMLQANALGLRDDDGEESDDELFGEKKIDEEGEKVKKALLKTDLGAL 516
EE+NK+ + +IKKEML AN G D D + ++ ++++ EG+K+++ L +
Sbjct: 284 EEDNKKLKDKIKKEMLTANLFGEADQDVDLEEE---NDRQMSREGKKLQRYLKLLEKNLA 340
Query: 517 YESDAE-ENPYLS 528
YESD E E+PY S
Sbjct: 341 YESDEEDEDPYAS 353
>At4g12610 [K] KOG2393 Transcription initiation factor IIF large subunit
(RAP74)
Length = 649
Score = 63.9 bits (154), Expect = 8e-10
Identities = 94/458 (20%), Positives = 164/458 (35%), Gaps = 81/458 (17%)
Query: 289 FRRGN----SKRKTRQLKRLDEEAKRLRFEEFYPWVMEDFDGFNTWVGSYEAGNSDSYVL 344
F++G+ S RK R +A+R + + PW++ED G + G E S +Y L
Sbjct: 83 FKKGSENKWSLRKDIPQGRQFTDAQREKLKN-KPWILEDETGQFQYQGHLEGSQSATYYL 141
Query: 345 LSVEDDGSFTMIPADKVYRFTARNKYNTLTIEEAEKRME---KNKEGAPRWFMK------ 395
L +++ F IPA Y F +Y LT+EEAE++M+ K +G RW MK
Sbjct: 142 LVMQNK-EFVAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRRKTADGYQRWMMKAANNGP 200
Query: 396 ----HLDESSKXXXXXXXXXXXXXAVDGNTRQAEDDEDRGDNSEVXXXXXXXXXXXXXXX 451
+D + G + + DRGD E
Sbjct: 201 ALFGEVDNEKESGGTSGGGGRGRKKSSGGDEEEGNVSDRGDEDEEEEASRKSRLGLNRKS 260
Query: 452 XXXGNEE----------------------------------ENKESEQRIKKEMLQANAL 477
+EE + E + + + A
Sbjct: 261 NDDDDEEGPRGGDLDMDDDDIEKGDDWEHEEIFTDDDEAVGNDPEEREDLLAPEIPAPPE 320
Query: 478 GLRDDDGEESDDELFGEKKIDEEGEKVKKALLKTDLGALYESDAEENPYLSHXXXXXXXX 537
+D+D EE+++E E + + G+++KK L K + L ESD +++
Sbjct: 321 IKQDEDDEENEEE---EGGLSKSGKELKKLLGKAN--GLDESDEDDDDDSDDEEETNYGT 375
Query: 538 XXXXXXXXXXXXXXXRRSSPKKKASDTPSGPP----------------KLVVKSINQNIV 581
+ K S P G P K S+ + +
Sbjct: 376 VTNSKQKEAAKEEPVDNAPAKPAPSGPPRGTPPAKPSKGKRKLNDGDSKKPSSSVQKKVK 435
Query: 582 VLTGEKSVLSNFPAGAWN-PNLKKRPAESQPQESMDNKRAKSDHSADDLLTEDDIINAI- 639
KS L A + N + +++P + A S +A +TED+I +
Sbjct: 436 TENDPKSSLKEERANTVSKSNTPTKAVKAEPASA----PASSSSAATGPVTEDEIRAVLM 491
Query: 640 EGKQLTLKQLIRQLKEKVGRHPDNKERMKVFVKKLVKL 677
E KQ+T + L+ + K ++ + ++K ++K+ K+
Sbjct: 492 EKKQVTTQDLVSRFKARL-KTKEDKNAFANILRKISKI 528
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.309 0.130 0.360
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,782,750
Number of Sequences: 60738
Number of extensions: 1713108
Number of successful extensions: 6063
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 6038
Number of HSP's gapped (non-prelim): 8
length of query: 686
length of database: 30,389,216
effective HSP length: 113
effective length of query: 573
effective length of database: 23,525,822
effective search space: 13480296006
effective search space used: 13480296006
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)