ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactVI4166   suspect: LH   U     KOG4697  Intracellular trafficking, secretion, and vesicular transport  Integral membrane protein involved in transport between the late Golgi and endosome

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI4166 1454175 1453585 -197 
         (197 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YJL004c [U] KOG4697 Integral membrane protein involved in transp...   263   9e-71
7299060 [U] KOG4697 Integral membrane protein involved in transp...    59   3e-09

>YJL004c [U] KOG4697 Integral membrane protein involved in transport between
           the late Golgi and endosome
          Length = 203

 Score =  263 bits (673), Expect = 9e-71
 Identities = 123/202 (60%), Positives = 156/202 (76%), Gaps = 6/202 (2%)

Query: 1   MASLRRYMRVPRELLPTEIFKQESQSPGKIIVQIILLQMFYYLTASTLFYCWAKICGYEI 60
           M S+RRY+RVP EL P++IFKQ+S SP KI +QI+LLQ+FYY TA  LFYCWAK+ GY++
Sbjct: 1   MVSIRRYLRVPNELKPSQIFKQDSLSPSKIGLQIVLLQIFYYTTAIVLFYCWAKLAGYDL 60

Query: 61  KLKQWLFTWQDIDFSNSFGISLSALWLIDSLICVFFLTIIVGRSKLAWDFAITIHAINLV 120
            +K+WLF+W++IDF+N++G+S+S LWL+DSLICVFFLT+IVGRSKLAWDFAITIHAIN +
Sbjct: 61  NIKEWLFSWENIDFTNAYGLSISLLWLLDSLICVFFLTVIVGRSKLAWDFAITIHAINFI 120

Query: 121 VVWTHTGLFPSLAWFILQFLSTLILIFLGTWITRWRELKDTFFEGMXXXXXXXXXXXXXX 180
           VV+ +T  FPS +WF LQ LS+LILIFLGTW TRWREL+DTFFEG+              
Sbjct: 121 VVFLYTRKFPSFSWFFLQILSSLILIFLGTWTTRWRELRDTFFEGLVDPNEGEVGLVTPS 180

Query: 181 XXXXGKS------IELRDLEAQ 196
                 S      I+L+DLE+Q
Sbjct: 181 QQHSNHSELEQSPIQLKDLESQ 202


>7299060 [U] KOG4697 Integral membrane protein involved in transport between
           the late Golgi and endosome
          Length = 160

 Score = 59.3 bits (142), Expect = 3e-09
 Identities = 36/142 (25%), Positives = 69/142 (48%), Gaps = 2/142 (1%)

Query: 20  FKQESQSPGKIIVQIILLQMFYYLTASTLFYCWAKICGYEIKLKQWLFTWQDIDFSNSFG 79
           F+     P  +  QI+ +Q   Y T   L +   K+ G    L   LF + +I   +  G
Sbjct: 6   FRNTQWDPTLLSSQIVSMQFCVYFTLGLLVFVANKLSGDNYSLDH-LFEYHEIHIYDMGG 64

Query: 80  ISLSALWLIDSLICVFFLTIIVGRSKLAWDFAITIHAINLVVVWTHTGLFPSLA-WFILQ 138
             +   +++++ +    L  IV R+KL  DF+ T H ++L++ W +   FP+ A W++L 
Sbjct: 65  RLVICAFVLNAFLASLALWCIVRRAKLCLDFSCTFHVLHLLICWWYNRSFPANASWWLLN 124

Query: 139 FLSTLILIFLGTWITRWRELKD 160
            ++  I+   G ++    E+K+
Sbjct: 125 VITGTIMCIGGEFLCLQTEMKE 146


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.331    0.143    0.469 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,132,004
Number of Sequences: 60738
Number of extensions: 365370
Number of successful extensions: 1038
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1035
Number of HSP's gapped (non-prelim): 2
length of query: 197
length of database: 30,389,216
effective HSP length: 101
effective length of query: 96
effective length of database: 24,254,678
effective search space: 2328449088
effective search space used: 2328449088
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)