ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI4318 good S KOG4020 Function unknown Protein DRE2, required for cell viability
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI4318 1505652 1504624 -343
(343 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKR071c [S] KOG4020 Protein DRE2 required for cell viability 304 1e-82
SPBC337.10c [S] KOG4020 Protein DRE2 required for cell viability 111 2e-24
CE01413 [S] KOG4020 Protein DRE2 required for cell viability 85 1e-16
7298199 [S] KOG4020 Protein DRE2 required for cell viability 84 4e-16
Hs10092673 [S] KOG4020 Protein DRE2 required for cell viability 83 5e-16
At5g18400 [S] KOG4020 Protein DRE2 required for cell viability 77 5e-14
Hs22054773 [S] KOG4020 Protein DRE2 required for cell viability 72 2e-12
ECU02g0490 [S] KOG4020 Protein DRE2 required for cell viability 71 3e-12
>YKR071c [S] KOG4020 Protein DRE2 required for cell viability
Length = 348
Score = 304 bits (778), Expect = 1e-82
Identities = 170/354 (48%), Positives = 225/354 (63%), Gaps = 17/354 (4%)
Query: 1 MTVSRDSLVLIHPAVTQQPELLETIEKGSILSETTIVAQYLINKLNDGTISPDDEKYDVI 60
M+ + L+LIHPAVT PEL+E + + + V Q+LINKLNDG+I+ ++ KY+ +
Sbjct: 1 MSQYKTGLLLIHPAVTTTPELVENTKAQAASKKVKFVDQFLINKLNDGSITLENAKYETV 60
Query: 61 YYVTPEKPEAIQFPPKLIPVLHKTLKPSGRLYGLSDTLKVDALINGFEIVSNNGSEYYWV 120
+Y+TPE I+FP KLI VL +LKP+G L GLSD KVDALINGFEI+ N +Y W+
Sbjct: 61 HYLTPEAQTDIKFPKKLISVLADSLKPNGSLIGLSDIYKVDALINGFEII--NEPDYCWI 118
Query: 121 K-ESREKKAPVSISLKGNMKNGASAPVA--LPSFKKLNKTQPIGSSPSAGLAVSXXXXXX 177
K +S + VSI LK N LP+FKK + + + PS A
Sbjct: 119 KMDSSKLNQTVSIPLKKKKTNNTKLQSGSKLPTFKKASSST--SNLPSFKKADHSRQPIV 176
Query: 178 XXXXXXXVSAIKLTXX------XXXXXXXXXXXXXXANNSKTKFFDDFDDPETGDSIDED 231
+ K+T +++SK ++FD D + DSI+E+
Sbjct: 177 KETDSFKPPSFKMTTEPKVYRVVDDLIEDSDDDDFSSDSSKAQYFDQVDTSD--DSIEEE 234
Query: 232 DLIAE--TEEDTITMIQCGKSKQRRRKACKDCSCGLKEMEEQEIESRRAKQQQVIKFSEE 289
+LI E + + ITMI CGKSK +++KACKDC+CG+KE EE EI R++Q +V+KF+E+
Sbjct: 235 ELIDEDGSGKSMITMITCGKSKTKKKKACKDCTCGMKEQEENEINDIRSQQDKVVKFTED 294
Query: 290 ELTEIDFTIEGKKVGGCGSCALGDAFRCSGCPYLGLPAFKPGQSINLNSISDDL 343
ELTEIDFTI+GKKVGGCGSC+LGDAFRCSGCPYLGLPAFKPGQ INL+SISDDL
Sbjct: 295 ELTEIDFTIDGKKVGGCGSCSLGDAFRCSGCPYLGLPAFKPGQPINLDSISDDL 348
>SPBC337.10c [S] KOG4020 Protein DRE2 required for cell viability
Length = 288
Score = 111 bits (277), Expect = 2e-24
Identities = 60/145 (41%), Positives = 90/145 (61%), Gaps = 15/145 (10%)
Query: 211 SKTKFFD----DFDDPETGDS----IDEDDLIAETEEDTITMI-QCGKSKQRRRKACKDC 261
SK K D D ++ +GD IDED+L+ E+ D + + +C ++++ACK+C
Sbjct: 140 SKNKILDFLKSDKENLISGDDDQELIDEDELLDESAHDNVLKVPECKPEPGKKKRACKNC 199
Query: 262 SCGLKEMEEQEIESRRAKQQQVIKFSEEELTEIDFTIEGKK---VGGCGSCALGDAFRCS 318
+CGL+EMEE E S+ + Q + +K ++ TE+DFT + K V CG+C LGDAFRCS
Sbjct: 200 TCGLREMEEHE-SSKTSAQLEAVKLTDT--TEVDFTEKLKSKNAVSSCGNCYLGDAFRCS 256
Query: 319 GCPYLGLPAFKPGQSINLNSISDDL 343
GCPY+G+PAF PG ++ L D +
Sbjct: 257 GCPYIGMPAFNPGDTVILAENRDKM 281
>CE01413 [S] KOG4020 Protein DRE2 required for cell viability
Length = 238
Score = 85.1 bits (209), Expect = 1e-16
Identities = 47/122 (38%), Positives = 65/122 (52%), Gaps = 27/122 (22%)
Query: 226 DSIDEDDLIAETEEDTITMIQ-----CGKSKQRRRK-ACKDCSCGLKEMEEQEIESRRAK 279
D IDED L+ E + + T Q CG ++K ACK+CSCGL E EE E + A
Sbjct: 137 DLIDEDGLLQEEDFEKPTGDQLKAGGCGPDDPNKKKRACKNCSCGLAEQEELEKMGQIAA 196
Query: 280 QQQVIKFSEEELTEIDFTIEGKKVGGCGSCALGDAFRCSGCPYLGLPAFKPGQSINLNSI 339
+ + CG+C+LGDAFRCS CPYLG P FKPG+++ ++++
Sbjct: 197 EPK---------------------SSCGNCSLGDAFRCSTCPYLGQPPFKPGETVKISTV 235
Query: 340 SD 341
D
Sbjct: 236 DD 237
>7298199 [S] KOG4020 Protein DRE2 required for cell viability
Length = 248
Score = 83.6 bits (205), Expect = 4e-16
Identities = 51/129 (39%), Positives = 69/129 (52%), Gaps = 30/129 (23%)
Query: 220 DDPETGDSIDEDDLIAETEE---DTITMIQCGKSKQRRRKACKDCSCGLKEMEEQEIESR 276
DD E IDE++L+ E ++ D + C S +RKACK+CSCGL E E E +S+
Sbjct: 145 DDEEL---IDEEELLDEEDKQKPDPAGLRVC--STTGKRKACKNCSCGLAEELETEKQSQ 199
Query: 277 RAKQQQVIKFSEEELTEIDFTIEGKKVGGCGSCALGDAFRCSGCPYLGLPAFKPGQSINL 336
+A + CG+C LGDAFRCS CPYLG+PAFKPG+ + L
Sbjct: 200 KATENAK--------------------SSCGNCYLGDAFRCSTCPYLGMPAFKPGEKVQL 239
Query: 337 --NSISDDL 343
N + D+
Sbjct: 240 ADNLLKSDI 248
>Hs10092673 [S] KOG4020 Protein DRE2 required for cell viability
Length = 367
Score = 83.2 bits (204), Expect = 5e-16
Identities = 43/117 (36%), Positives = 66/117 (55%), Gaps = 27/117 (23%)
Query: 226 DSIDEDDLI-----AETEEDTITMIQCGKSKQRRRKACKDCSCGLKEMEEQEIESRRAKQ 280
D ID D+L+ + + ++ CG+ K +RKACK+C+CGL E E+E ++++
Sbjct: 266 DLIDSDELLDPEDLKKPDPASLRAASCGEGK--KRKACKNCTCGLAE----ELEKEKSRE 319
Query: 281 QQVIKFSEEELTEIDFTIEGKKVGGCGSCALGDAFRCSGCPYLGLPAFKPGQSINLN 337
Q + + CG+C LGDAFRC+ CPYLG+PAFKPG+ + L+
Sbjct: 320 Q----------------MSSQPKSACGNCYLGDAFRCASCPYLGMPAFKPGEKVLLS 360
>At5g18400 [S] KOG4020 Protein DRE2 required for cell viability
Length = 374
Score = 76.6 bits (187), Expect = 5e-14
Identities = 47/121 (38%), Positives = 62/121 (50%), Gaps = 22/121 (18%)
Query: 218 DFDDPETGDSIDEDDLIAETEEDTIT-MIQCGKSKQRRRKACKDCSCGLKEMEEQEIESR 276
D DD D IDED L+ TEED + + + +KACK+C CG E+EE+ ++
Sbjct: 267 DLDDDV--DLIDEDSLL--TEEDLMKPQLPVASGCETTKKACKNCVCGRAEIEEKAVKLG 322
Query: 277 RAKQQQVIKFSEEELTEIDFTIEGKKVGGCGSCALGDAFRCSGCPYLGLPAFKPGQSINL 336
+ Q IE + CGSC LGDAFRC CPY GLP FK G+ + L
Sbjct: 323 LTEDQ----------------IENPQ-SSCGSCGLGDAFRCGTCPYKGLPPFKLGEKVTL 365
Query: 337 N 337
+
Sbjct: 366 S 366
>Hs22054773 [S] KOG4020 Protein DRE2 required for cell viability
Length = 248
Score = 71.6 bits (174), Expect = 2e-12
Identities = 38/124 (30%), Positives = 67/124 (53%), Gaps = 23/124 (18%)
Query: 217 DDFDDPETGDSIDEDDLIAETEEDTITMIQCGKSKQRRRKACKDCSCGLKEMEEQEIESR 276
D D ++ + +D +DL + + ++ CG+ K +RK C+ C+ GL E E+E
Sbjct: 144 DSMDLIDSEELLDPEDL-KKPDPTSLQAAPCGEGK--KRKTCQSCTRGLAE----ELEKE 196
Query: 277 RAKQQQVIKFSEEELTEIDFTIEGKKVGGCGSCALGDAFRCSGCPYLGLPAFKPGQSINL 336
++++Q + + CG+C LG++F C+ CPYLG+PAFKPG+ + L
Sbjct: 197 KSREQ----------------MSSQPKSACGNCYLGNSFHCASCPYLGIPAFKPGEKVLL 240
Query: 337 NSIS 340
++ S
Sbjct: 241 SNSS 244
>ECU02g0490 [S] KOG4020 Protein DRE2 required for cell viability
Length = 112
Score = 70.9 bits (172), Expect = 3e-12
Identities = 39/101 (38%), Positives = 53/101 (51%), Gaps = 31/101 (30%)
Query: 237 TEEDTITMIQCGKSKQRRRKACKDCSCGLKEMEEQEIESRRAKQQQVIKFSEEELTEIDF 296
T+ED IQ + +++ACKDC+CGLKE EQE+ +R A
Sbjct: 31 TDEDKA--IQRSERPPAKKRACKDCTCGLKE--EQEVRTRSA------------------ 68
Query: 297 TIEGKKVGGCGSCALGDAFRCSGCPYLGLPAFKPGQSINLN 337
CG+C GDAFRCSGCP LGLP ++PG ++ +
Sbjct: 69 ---------CGNCYKGDAFRCSGCPSLGLPPYEPGDVVSFS 100
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.312 0.133 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,091,271
Number of Sequences: 60738
Number of extensions: 864464
Number of successful extensions: 3045
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3027
Number of HSP's gapped (non-prelim): 9
length of query: 343
length of database: 30,389,216
effective HSP length: 107
effective length of query: 236
effective length of database: 23,890,250
effective search space: 5638099000
effective search space used: 5638099000
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)