ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI4329 good O KOG2463 Posttranslational modification, protein turnover, chaperones Predicted RNA-binding protein Nob1p involved in 26S proteasome assembly
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI4329 1510381 1508966 -472
(472 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR056c [O] KOG2463 Predicted RNA-binding protein Nob1p involved... 551 e-157
SPAC1486.09 [O] KOG2463 Predicted RNA-binding protein Nob1p invo... 198 2e-50
7291118 [O] KOG2463 Predicted RNA-binding protein Nob1p involved... 116 8e-26
CE27481 [O] KOG2463 Predicted RNA-binding protein Nob1p involved... 104 3e-22
Hs7661532 [O] KOG2463 Predicted RNA-binding protein Nob1p involv... 100 5e-21
At5g41190 [O] KOG2463 Predicted RNA-binding protein Nob1p involv... 62 2e-09
>YOR056c [O] KOG2463 Predicted RNA-binding protein Nob1p involved in 26S
proteasome assembly
Length = 459
Score = 551 bits (1421), Expect = e-157
Identities = 280/472 (59%), Positives = 341/472 (71%), Gaps = 21/472 (4%)
Query: 4 QHPHVKALVLDATPLITQSYTHYQNYAETFYTAPTVLHEIKDAKSRKNLEIWQSLGTLKV 63
Q HV+AL+LDATPLITQSYTHYQNYA++FYT PTV EIKDA++RKNLEIWQSLGTLK+
Sbjct: 5 QTAHVRALILDATPLITQSYTHYQNYAQSFYTTPTVFQEIKDAQARKNLEIWQSLGTLKL 64
Query: 64 KHPSAASIKRVSDFAKLTGDYSVLSANDIHIIALAYELETELNKGDWRLRKRPGEALKHE 123
HPS SI +VS FAKLTGDYSVLSAND+HI+AL YELE +LN GDWRLRK+PG+AL
Sbjct: 65 VHPSENSIAKVSTFAKLTGDYSVLSANDLHILALTYELEIKLNNGDWRLRKKPGDALD-A 123
Query: 124 TKEETRPKETIEQTQDPTAESDTAETPVXXXXXXXXXXXXXXXXXXXXXXXSQETTAEHQ 183
+K + + T+D E D+ P ++ A+ +
Sbjct: 124 SKADVGTDGKQKLTEDNKKEEDSESVP----------------KKKNKRRGGKKQKAKRE 167
Query: 184 PTESQEPANEEQEQPILVDEVSPDVPGEEEV--ETATEAKNDEEEEGFLSDEDGEWITPD 241
E++E N E +E + +E++ + + +D E +D+DG+WITP+
Sbjct: 168 AREAREAENANLELESKAEEHVEEAGSKEQICNDENIKESSDLNEVFEDADDDGDWITPE 227
Query: 242 NLTEAIIKDSGEDTTGGEGV-VSENNTASSLELPQNQVALATGDFAXXXXXXXXXXXXXX 300
NLTEAIIKDSGEDTTG GV SE + +L P+NQVALATGDFA
Sbjct: 228 NLTEAIIKDSGEDTTGSLGVEASEEDRHVALNRPENQVALATGDFAVQNVALQMNLNLMN 287
Query: 301 FMSGLRIKKLRNYMLRCHACFQLLPMPKDGKAKHFCPSCGGSGTVLRCAVSVDSVTGKVT 360
FMSGL+IK++RNYMLRCHACF++ P+PKDGK KHFC SCGG GT+LRCAVSVDS TG VT
Sbjct: 288 FMSGLKIKRIRNYMLRCHACFKIFPLPKDGKPKHFCASCGGQGTLLRCAVSVDSRTGNVT 347
Query: 361 PHLKANFQWNNRGNRYSLPSPLSKNAQKRFGNKGFVHSKHSQDVELLREDQKEYEKAVKQ 420
PHLK+NFQWNNRGNRYS+ SPLSKN+QKR+G KG VHSK ++V +LREDQKEYEK +KQ
Sbjct: 348 PHLKSNFQWNNRGNRYSVASPLSKNSQKRYGKKGHVHSKPQENV-ILREDQKEYEKVIKQ 406
Query: 421 EEWTRRHNEKILNNWIGGGSAENYISPFAIDGLKHHNVRVGRGRFANSTRKR 472
EEWTRRHNEKILNNWIGGGSA+NYISPFAI GLK HNVR+G+GR+ NS+++R
Sbjct: 407 EEWTRRHNEKILNNWIGGGSADNYISPFAITGLKQHNVRIGKGRYVNSSKRR 458
>SPAC1486.09 [O] KOG2463 Predicted RNA-binding protein Nob1p involved in 26S
proteasome assembly
Length = 388
Score = 198 bits (503), Expect = 2e-50
Identities = 147/411 (35%), Positives = 203/411 (48%), Gaps = 77/411 (18%)
Query: 11 LVLDATPLITQSYTHYQNYAETFYTAPTVLHEIKDAKSRKNLEIWQSLGTLKVKHPSAAS 70
LVLD +I S T +N AE+FYT P V+ EI+D SRKN E+W +V P
Sbjct: 8 LVLDTGGIICSS-TLLRNSAESFYTIPRVIAEIRDETSRKNFELWGDQVIQRVPKPEF-- 64
Query: 71 IKRVSDFAKLTGDYSVLSANDIHIIALAYELETELNKGDWRLRKRPGEALKHETKEETRP 130
IK+VS+FAK TGDYS LS DI I+AL YELE E N GDWRLRK PG+ KH +
Sbjct: 65 IKKVSEFAKQTGDYSSLSVTDIQILALTYELEVEFNGGDWRLRKYPGQ--KHINGKPPSN 122
Query: 131 KETIEQTQDPTAESDTAETPVXXXXXXXXXXXXXXXXXXXXXXXSQETTAEHQPTESQEP 190
+ E PT+ SDTA + TE+ +P
Sbjct: 123 SNSTEDASKPTS-SDTASV---------------------------------KETENSDP 148
Query: 191 ANEEQEQPILVDEVSPDVPGEEEVETATEAKNDEEEEGFLSDEDGEWITPDNLTEAIIKD 250
+ E E +++ + +E TE +N E+E+ DED WITP N I K
Sbjct: 149 KSAENE---VLEGETTQHSNNKEAHPNTE-ENKEQEDNEEDDEDDGWITPSN----IRKK 200
Query: 251 SGEDTTGGEGVVSENNTASSLELPQN-QVALATGDFAXXXXXXXXXXXXXXFMSGLRIKK 309
ED G SL P++ +VA AT DF+ G +I+
Sbjct: 201 KAEDGVG-----------ESLVQPKHLKVACATTDFSMQNVLLQIGLNLVS-SDGFKIQN 248
Query: 310 LRNYMLRCHACFQLLPMPKDGKAKHFCPSCGGSGTVLRCAVSVDSVTGKVTPHLKANFQW 369
++ ++LRCH C+ ++ KD K FCPSCGG+ T+++ S++S G+ HLK NF+W
Sbjct: 249 VKRFVLRCHGCYTVV---KD-MEKKFCPSCGGN-TLIKTTCSINS-KGEFQVHLKKNFEW 302
Query: 370 NNRGNRYSLPSPLSKNAQKRFGNKGFVHSKHSQDVELLREDQKEYEKAVKQ 420
RG +YSLP P+ + + G K +LREDQ EY++AV++
Sbjct: 303 KTRGTKYSLPKPVHGTSNGK-GKKN----------PVLREDQPEYQRAVRR 342
>7291118 [O] KOG2463 Predicted RNA-binding protein Nob1p involved in 26S
proteasome assembly
Length = 473
Score = 116 bits (290), Expect = 8e-26
Identities = 124/447 (27%), Positives = 180/447 (39%), Gaps = 80/447 (17%)
Query: 1 MAEQHPHVKALVLDATPLITQSYTHYQNYAETFYTAPTVLHEIKDAKSRKNLEIWQSLGT 60
MAE +K LV D T I + Q YAE T P V+ E+++ + + L +
Sbjct: 1 MAETSEKIKFLVADTTAFINAVPLN-QEYAEQVLTVPDVVAEVRNKRQIRRLCVLPF--D 57
Query: 61 LKVKHPSAASIKRVSDFAKLTGDYSVLSANDIHIIALAYELETELNKGDWRLRKRPGEAL 120
L+V+ P SIK +FAK TGDY+ LS D+ +I+L YELE + N G LR+ P A
Sbjct: 58 LQVREPRPESIKHCVEFAKKTGDYASLSGIDLKVISLTYELEAD-NVGTDHLRQEPVMAQ 116
Query: 121 KHETKE---------------------ETRPKETIEQTQDPTAESDTAETPVXXXXXXXX 159
+K+ E +E E+ D + E D AE
Sbjct: 117 TIASKDKPEEMQDANNKRLVGWYMPEGEEEEEEDSEEEDDYSQEEDDAEGDEQEANESPD 176
Query: 160 XXXXXXXXXXXXXXXSQETTAEHQPTESQEPANEEQEQ--------PILVDE-------V 204
T E + + QEP+ EE ++ P +E V
Sbjct: 177 TVKAAIEAQLAGKEPFASETIEKEEDQDQEPSQEELDKLFEQLKCAPSAEEEKELSELLV 236
Query: 205 SPDVPGEEEVETATEAKNDEEEEGFLSDEDGEWITPDNLTEAIIKDSGEDTTGGEGVVSE 264
S +EEV EAK + + + D+ WIT N+ +A G+ T
Sbjct: 237 SQPAEVDEEVSEPQEAKQSQNLDEDVGDDG--WITHSNIKKAKKALEGKVETD------- 287
Query: 265 NNTASSLELPQNQVALATGDFAXXXXXXXXXXXXXXFMSGLRIKKLRNYMLRCHACFQLL 324
E+P VA T D+A ++G IK+LR Y+LRC+AC++
Sbjct: 288 -------EVP--PVACMTTDYALQNVLKQLNLHLAA-LNGRIIKQLRTYILRCYACYKTT 337
Query: 325 PMPKDGKAKHFCPSCGGSGTVLRCAVSVDSVTGKVTPHLKANFQWNNRGNRYSLPSPLSK 384
+ K FCP+CG T+ R AVS+D G+ H+ N+ SLP
Sbjct: 338 SI----MTKVFCPNCGNK-TLKRVAVSLDE-NGRQVIHINTRRPLTNKYKNQSLPR---- 387
Query: 385 NAQKRFGNKGFVHSKHSQDVELLREDQ 411
F KHS++ +L EDQ
Sbjct: 388 ----------FQGGKHSRN-PILFEDQ 403
>CE27481 [O] KOG2463 Predicted RNA-binding protein Nob1p involved in 26S
proteasome assembly
Length = 364
Score = 104 bits (259), Expect = 3e-22
Identities = 99/383 (25%), Positives = 151/383 (38%), Gaps = 93/383 (24%)
Query: 1 MAEQHPHVKALVLDATPLITQSYTHYQNYAETFYTAPTVLHEIKDAKSRKNLEIWQSLG- 59
M + +K LV+D +I H A+ +Y P +L E++ + +RK +W +L
Sbjct: 1 MTNKEKPIKHLVIDTCAIIANRNLHA--LADHYYAPPQILDELRSSAARK---LWNTLPF 55
Query: 60 TLKVKHPSAASIKRVSDFAKLTGDYSVLSANDIHIIALAYELETELNKGDWRLRKRPGEA 119
+ ++ P+ +++ V D +K TGD+ LS DI +IAL Y+L +
Sbjct: 56 EVTLREPTQTALRAVIDASKTTGDFQSLSMVDIKMIALTYDLHRQY-------------- 101
Query: 120 LKHETKEETRPKETIEQTQDPTAESDTAETPVXXXXXXXXXXXXXXXXXXXXXXXSQETT 179
AE+D AET Q T
Sbjct: 102 ---------------------VAENDVAET---------TEDDEKKNIEDLSAKVEQVTV 131
Query: 180 AEHQPTESQEPANEEQEQPILVDEVSPDVPGEEEVETATEAKNDEEEEGFLSDEDGEWIT 239
E+ +E E +E E+ + +++ E E F DEDG WIT
Sbjct: 132 GENTEDTKEEEIEENEES--------------EDSAVSEGSESSESSESF--DEDG-WIT 174
Query: 240 PDNLTEAIIKDSGEDTTGGEGVVSENNTASSLELPQNQ-VALATGDFAXXXXXXXXXXXX 298
DN+ E + K + E+ +N V T DFA
Sbjct: 175 QDNIEETLKK------------------LGAFEIEENMLVGCLTTDFALQNVLLAMNLSL 216
Query: 299 XXFMSGLRIKKLRNYMLRCHACFQLLPMPKDGKAKHFCPSCGGSGTVLRCAVSVDSVTGK 358
+SG RI+KL++++LRC CF + K FCP+CG T+ +CAVSVD G
Sbjct: 217 VS-LSGYRIRKLKSFVLRCRTCFSTTSV----MTKEFCPACGHK-TLHKCAVSVDE-DGN 269
Query: 359 VTPHLKANFQWNNRGNRYSLPSP 381
H+ N N RG Y+L +P
Sbjct: 270 QQLHINWNRMANRRGLVYTLANP 292
>Hs7661532 [O] KOG2463 Predicted RNA-binding protein Nob1p involved in 26S
proteasome assembly
Length = 412
Score = 100 bits (249), Expect = 5e-21
Identities = 103/389 (26%), Positives = 160/389 (40%), Gaps = 78/389 (20%)
Query: 8 VKALVLDATPLITQSYTHYQNYAETFYTAPTVLHEIKDAKSRKNLEIWQSLGTLKVKHPS 67
V+ +V DA + + Q+ + YT V+ EI+D +R+ L + L+ K P
Sbjct: 4 VEHVVADAGAFLR--HAALQDIGKNIYTIREVVTEIRDKATRRRLAVLPY--ELRFKEPL 59
Query: 68 AASIKRVSDFAKLTGDYSVLSANDIHIIALAYELETELNKGDWRLRKRPGEALKHETKEE 127
++ V++F+K TGDY LSA DI ++AL Y+LE E + H K+E
Sbjct: 60 PEYVRLVTEFSKKTGDYPSLSATDIQVLALTYQLEAEF------------VGVSH-LKQE 106
Query: 128 TRPKETIEQTQDPTAESDTAETPVXXXXXXXXXXXXXXXXXXXXXXXSQETTAEHQPTES 187
+ + Q P ETP+ QET H E
Sbjct: 107 PQKVKVSSSIQHP-------ETPLHISGFHLPYKPKP----------PQETEKGHSACEP 149
Query: 188 QE-------------PANEEQEQPILVDEVSPDVPGEEEVETATEAKNDEEEEGFLSDED 234
+ P + + Q +L+D DVP EEE E E E+ + D+
Sbjct: 150 ENLEFSSFMFWRNPLPNIDHELQELLIDR-GEDVPSEEEEE---EENGFEDRKDDSDDDG 205
Query: 235 GEWITPDNLTEAIIKDSGEDTTGGEGVVSENNTASSLELPQN-QVALATGDFAXXXXXXX 293
G WITP N+ + ++P++ +V T DFA
Sbjct: 206 GGWITPSNIKQI------------------QQELEQCDVPEDVRVGCLTTDFAMQNVLLQ 247
Query: 294 XXXXXXXFMSGLRIKKLRNYMLRCHACFQLLPMPKDGKAKHFCPSCGGSGTVLRCAVSVD 353
++G+ I++ R+Y+LRCH CF+ ++ FC CG T+ + +V+V
Sbjct: 248 MGLHVLA-VNGMLIREARSYILRCHGCFK----TTSDMSRVFCSHCGNK-TLKKVSVTV- 300
Query: 354 SVTGKVTPHLKANFQ-WNNRGNRYSLPSP 381
S G + H N + N RG RYSLP+P
Sbjct: 301 SDDGTLHMHFSRNPKVLNPRGLRYSLPTP 329
>At5g41190 [O] KOG2463 Predicted RNA-binding protein Nob1p involved in 26S
proteasome assembly
Length = 602
Score = 62.0 bits (149), Expect = 2e-09
Identities = 57/215 (26%), Positives = 89/215 (40%), Gaps = 20/215 (9%)
Query: 179 TAEHQPTESQEPA-NEEQEQPILVD----EVSPDVPGEEEVETATEAK----NDEEEEGF 229
T E T +++ N E IL D E S EE ET EA D+ +
Sbjct: 313 TKEANETHAKDSGKNGEDISSILKDMRLEEESLRALQEETEETNAEATLINGEDDIDHDI 372
Query: 230 LSDEDGEWITPDNLTEAIIKDSGEDTTGGEGVVSENNTASSLEL---PQNQVALATGDFA 286
+ +G + L I EDT + + ++ S L ++ VA TGD+A
Sbjct: 373 EVEAEGIDVANQALENLEIASEAEDTFEASSIGDDGSSEQSWSLRALSESSVACITGDYA 432
Query: 287 XXXXXXXXXXXXXXFMSGLRIKKLRNYMLRCHACFQLLPMPKDGKAKHFCPSCGGSGTVL 346
G++I++L ++L+CHAC+ + P + FCP CG GT+
Sbjct: 433 MQNVILQMGLRLLA-PGGMQIRQLHRWILKCHACYTVTP----EIGRIFCPKCGNGGTLR 487
Query: 347 RCAVSVDSVTGKVTPHLKANFQWNNRGNRYSLPSP 381
+ AV++ G + K RG +YS+P P
Sbjct: 488 KVAVTI-GANGAIIAACKPRI--TLRGTQYSIPMP 519
Score = 60.1 bits (144), Expect = 7e-09
Identities = 39/100 (39%), Positives = 55/100 (55%), Gaps = 7/100 (7%)
Query: 8 VKALVLDATPLIT--QSYTHYQNYAETFYTAPTVLHEIKDAKSRKNLEIWQSLGTLKVKH 65
+ V+DA +I QS T N+A+ F T P VL EI+D SR+ L T+
Sbjct: 44 ISIAVVDANAIIEGRQSLT---NFADKFVTVPEVLSEIRDPASRRRLAFIPF--TIDTME 98
Query: 66 PSAASIKRVSDFAKLTGDYSVLSANDIHIIALAYELETEL 105
PS S+ +V FA+ TGD LS D+ +IAL+Y LE ++
Sbjct: 99 PSPESLSKVIKFARATGDLQSLSDVDLKLIALSYTLEAQV 138
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.310 0.129 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,541,615
Number of Sequences: 60738
Number of extensions: 1251247
Number of successful extensions: 6287
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 6260
Number of HSP's gapped (non-prelim): 10
length of query: 472
length of database: 30,389,216
effective HSP length: 110
effective length of query: 362
effective length of database: 23,708,036
effective search space: 8582309032
effective search space used: 8582309032
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)