ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI4351 good R KOG4372 General function prediction only Predicted alpha/beta hydrolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI4351 1518096 1516720 -459
(459 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR059c [R] KOG4372 Predicted alpha/beta hydrolase 353 4e-97
SPAC4A8.10 [R] KOG4372 Predicted alpha/beta hydrolase 78 2e-14
YDL109c [R] KOG4372 Predicted alpha/beta hydrolase 69 1e-11
YGL144c [R] KOG4372 Predicted alpha/beta hydrolase 66 9e-11
At5g51180 [R] KOG4372 Predicted alpha/beta hydrolase 59 2e-08
At1g10040 [R] KOG4372 Predicted alpha/beta hydrolase 57 8e-08
>YOR059c [R] KOG4372 Predicted alpha/beta hydrolase
Length = 450
Score = 353 bits (905), Expect = 4e-97
Identities = 197/465 (42%), Positives = 285/465 (60%), Gaps = 30/465 (6%)
Query: 4 TKKKHLFILVHGLWGNHKHMNSIKEMLEKTL---DDIDDIVIFKPENSGYLKTLHGIRVV 60
T KHLF+L+HGLWGN+ HM S++ +L TL D DD++ F P+ + KT GI ++
Sbjct: 2 TSDKHLFVLIHGLWGNYTHMESMRTILSTTLKKEDVNDDMIYFLPKQNAMFKTFDGIEII 61
Query: 61 SYNVLDEICKFVLNYGPEKFDRVSMIGYSMGGLVSRFIIGKMVTECRDIFQHMEPMIFMT 120
Y L E+C+F+ +Y K ++S++GYS GGLV+RF+IGKM+TE +++F+ +EP +F+T
Sbjct: 62 GYRTLIEVCEFIRDYKDGKITKLSVMGYSQGGLVARFMIGKMLTEFKELFEDIEPQLFIT 121
Query: 121 FATPHLGVNFYLPSDKTRRYVSRKILTSVLSGLGRTILGRSGAEIFISNKDDRILVDLSQ 180
ATPHLGV FY P+ + L S L LG TILG+SG E+FI+N + ILV LSQ
Sbjct: 122 MATPHLGVEFYNPTG----IAYKSALYSALRTLGSTILGKSGREMFIANSSNNILVKLSQ 177
Query: 181 GEYLYGLSRFHHRVCFANVKNDRTVAFYTSFITDSDPFIETGNNVRYHFNHNLPIDVSEY 240
GEYL LS F R+ FANVKNDRTVAFYT+FITD DPFI+ N ++Y F +P S Y
Sbjct: 178 GEYLEALSLFKWRIAFANVKNDRTVAFYTAFITDCDPFIDFDNKLKYTFEEKIP--GSGY 235
Query: 241 P-VQPRVLDLDSLDPAVDRIKENETIVTLKERFSRILLIMALMTFFFPLVFTVNVIGTCY 299
+ P+++DL++L+ V+ + T K+ IL+I+ P+ +N +GT Y
Sbjct: 236 KGILPKIVDLNALN--VNSHAPTKPTKTYKKWGRTILIILVATFLILPIALVMNGLGTAY 293
Query: 300 SYITTYHHHSLLRKGHWKLE-SGENGVFNTIMSSIRNVIGDAINDEQDSGKDQGSDWSIE 358
SYI T + +L G E G+ G+ + + + G IN S D +++
Sbjct: 294 SYIVTCKYRKMLSNGILHNEVRGKLGLTEQLKGYVTDAYGSIIN----SALDMDANYEAS 349
Query: 359 NNSLYHTLTNDSAQMNDVWKQFIRSHSK----EWKHTKFPKLPFDESRATIYQNLTKLTW 414
N++L + + WK+FI+ ++ WK +KF KLPFDE+R I +NL KL W
Sbjct: 350 NSNLVN-------EEELPWKEFIQKYTTINDGVWK-SKFKKLPFDENRKVILRNLNKLKW 401
Query: 415 IRVPVYIKTLNAHDGIVARKGLDSAT-PYGVANVEFACELINHLL 458
IRVP+YIK +NAH IVAR+G+D T G+A +EF +L+ +L+
Sbjct: 402 IRVPIYIKAVNAHGVIVARRGMDENTAATGIACIEFTAQLLAYLM 446
>SPAC4A8.10 [R] KOG4372 Predicted alpha/beta hydrolase
Length = 723
Score = 78.2 bits (191), Expect = 2e-14
Identities = 58/212 (27%), Positives = 100/212 (46%), Gaps = 12/212 (5%)
Query: 4 TKKKHLFILVHGLWGN-HKHMNSIKEMLEKTLDDIDDIVIFKPENSGYLKTLHGIRVVSY 62
+K HL +L HG+ N M +KE L ++ + ++V+ + Y +T G+R +
Sbjct: 240 SKNSHLVVLTHGMHSNVGADMEYLKEKLIESSKSVKELVVVRGFTGNYCQTEKGVRWLGK 299
Query: 63 NVLDEICKFVLNYGPEKFDR---VSMIGYSMGGLVSRFIIGKMVTECRD-IFQHMEPMIF 118
L E + +G F R +S++ +S+GGLV + +G + + FQ + P+ F
Sbjct: 300 R-LGEWLLDITGWGSASFPRYSHISVVAHSLGGLVQTYAVGYVHAKTHGAFFQAIHPVFF 358
Query: 119 MTFATPHLGVNFYLPSDKTRRYVSRKILTSVLSGLGRTILGRSGAEIFISNKDDRILVDL 178
+T ATP LGV PS Y+ + + ++ G+ L + I ++ +L+
Sbjct: 359 VTLATPWLGVAGEHPS-----YIGKALSYGIIGKTGQD-LSLTPLNHSIESRPFLVLMSD 412
Query: 179 SQGEYLYGLSRFHHRVCFANVKNDRTVAFYTS 210
+ +S F R+ FAN ND V F TS
Sbjct: 413 PSTPFFQAVSFFEKRILFANTTNDYIVPFGTS 444
>YDL109c [R] KOG4372 Predicted alpha/beta hydrolase
Length = 647
Score = 69.3 bits (168), Expect = 1e-11
Identities = 54/209 (25%), Positives = 100/209 (47%), Gaps = 15/209 (7%)
Query: 4 TKKKHLFILVHGLWGN-HKHMNSIKEMLEKT-LDDIDDIVIFKPENSGYLKTLHGIRVVS 61
++K HL IL HG N M + E + K +++ ++ ++ K +T GI+ +
Sbjct: 190 SQKSHLVILTHGFQSNVSADMEYLMEEIYKAQMNNPNERLVIKGYMKNACETEKGIKFLG 249
Query: 62 YNVLDEICKFVLNYGPEKFDRVSMIGYSMGGLVSRFIIGKMVTECRDIFQHMEPMIFMTF 121
+ + I + + + ++S IG+S+GGL F I + T+ F+ +EP+ F++
Sbjct: 250 VGLANYIIDELYD---DSVGKISFIGHSLGGLTQTFAICYIKTKYPYFFKKVEPINFISL 306
Query: 122 ATPHLGVNFYLPSDKTRRYVSRKILTSVLSGLGRTILGRSGAEIFISNKDDRILVDLSQG 181
A+P LG+ + T YV + ++ G+ + + G + D +L LS+
Sbjct: 307 ASPLLGI-----ATSTPNYVKMSLSMGIIGTTGQELGLKDG-----NYGDKPLLYLLSEE 356
Query: 182 EYLYGLSRFHHRVCFANVKNDRTVAFYTS 210
+ L+RF R +AN ND V Y+S
Sbjct: 357 SLISVLARFKRRTLYANAVNDGIVPLYSS 385
>YGL144c [R] KOG4372 Predicted alpha/beta hydrolase
Length = 685
Score = 66.2 bits (160), Expect = 9e-11
Identities = 57/214 (26%), Positives = 101/214 (46%), Gaps = 21/214 (9%)
Query: 2 TETKKKHLFILVHGLWGN-HKHMNSIKEMLEKTLDDID-DIVIFKPENSGYLKTLHGIRV 59
T KKKHL +L HGL N + I E + K + + ++ K +T G++
Sbjct: 183 TPQKKKHLVVLTHGLHSNVSTDLVYIMEQIYKAQKNYPHEQIVVKGYRGNVCQTEKGVKY 242
Query: 60 VSYNVLDEICKFVLNYGPEKFDRVSMIGYSMGGLVSRFIIGKMVTECRDIFQHMEPMIFM 119
+ + + I + + + E ++S +G+S+GGL+ F I + F+ + P+ F+
Sbjct: 243 LGTRLAEYIIQDLYD---ESIRKISFVGHSLGGLIQAFAIAYIYEVYPWFFKKVNPINFI 299
Query: 120 TFATPHLGVNFYLPSDKTRRYVSRKILTSVLSGLGRTILGRSGAEIFISNKDD---RILV 176
T A+P LG+ P+ Y+ K+L S ++G++G ++ + N + +L
Sbjct: 300 TLASPLLGIVTDNPA-----YI--KVLLSF------GVIGKTGQDLGLENDVEVGKPLLY 346
Query: 177 DLSQGEYLYGLSRFHHRVCFANVKNDRTVAFYTS 210
LS + L RF R +AN ND V YT+
Sbjct: 347 LLSGLPLIEILRRFKRRTVYANAINDGIVPLYTA 380
>At5g51180 [R] KOG4372 Predicted alpha/beta hydrolase
Length = 349
Score = 58.9 bits (141), Expect = 2e-08
Identities = 61/249 (24%), Positives = 104/249 (41%), Gaps = 47/249 (18%)
Query: 8 HLFILVHGLWGNHKHMNSIKEMLEKTLDDIDDIVIFKPENSGYLKTLHGIRVVSYNVLDE 67
HL ++VHG+ G+ E K + D + + E + TL G+ V+ + E
Sbjct: 35 HLVVMVHGILGSTDDWKFGAEQFVKKMPD--KVFVHCSEKNVSALTLDGVDVMGERLAAE 92
Query: 68 ----ICKFVLNYGPEKFDRVSMIGYSMGGLVSRFIIGKMVTECR---------DIFQH-- 112
ICK +S + +S+GGL +R+ IGK+ D Q
Sbjct: 93 RKPNICK------------ISFVAHSLGGLAARYAIGKLYKPANQEDVKDSVADSSQETP 140
Query: 113 ------MEPMIFMTFATPHLGVNFYLPSDKTRRYVSRKILTSVLSGLGRTILGRSGAEIF 166
+E M F+T ATPHLG + + + + V + I R+G +F
Sbjct: 141 KGTICGLEAMNFITVATPHLG---SMGNKQVPFLFGFSSIEKVAGLIIHWIFKRTGRHLF 197
Query: 167 ISNKDD-------RILVDLSQGEYLYGLSRFHHRVCFANVKNDRTVAFYTSFITDSD-PF 218
+ ++++ R++ D ++ L F RV ++NV +DR + + S DS+ P
Sbjct: 198 LKDEEEGKPPLLRRMVEDTDDCHFISALRAFKRRVAYSNVGHDR-ILYTASIRRDSELPK 256
Query: 219 IETGNNVRY 227
E N +Y
Sbjct: 257 WEDSLNEKY 265
>At1g10040 [R] KOG4372 Predicted alpha/beta hydrolase
Length = 379
Score = 56.6 bits (135), Expect = 8e-08
Identities = 48/190 (25%), Positives = 92/190 (48%), Gaps = 54/190 (28%)
Query: 4 TKKKHLFILVHGLWGNHKHMN-SIKEMLEKTLDDIDDIVIFKPENSGYLKTLHGIRVVSY 62
T+ HL ++V+GL G+ ++ + K+ML+K D+ ++ + + +T G+ V+
Sbjct: 77 TEPTHLVVMVNGLIGSAQNWRFAAKQMLKKYPQDL---LVHCSKRNHSTQTFDGVDVMGE 133
Query: 63 NVLDEICKFVLNYGPEKFDRVSMIGYSMGGLVSRFIIGKMV-TECRDIFQH--------- 112
+ +E ++S +G+S+GGL++R+ IG++ E R+ H
Sbjct: 134 RLAEE--------------KISFVGHSLGGLIARYAIGRLYEQESREELPHNSDDIGDKC 179
Query: 113 -----------MEPMIFMTFATPHLGVNFYLPSDKTRRYVSRKILTSVLSGLGRTILGRS 161
+EP+ F+T ATPHLG +R + R L + +SG LG++
Sbjct: 180 SIEEPKARIAGLEPVYFITSATPHLG---------SRGHKQR--LATRMSG----CLGKT 224
Query: 162 GAEIFISNKD 171
G +F+++ D
Sbjct: 225 GKHLFLADSD 234
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.323 0.139 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,397,468
Number of Sequences: 60738
Number of extensions: 1251954
Number of successful extensions: 3098
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 3079
Number of HSP's gapped (non-prelim): 7
length of query: 459
length of database: 30,389,216
effective HSP length: 110
effective length of query: 349
effective length of database: 23,708,036
effective search space: 8274104564
effective search space used: 8274104564
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)