ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI4418 normal R KOG4372 General function prediction only Predicted alpha/beta hydrolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI4418 1539612 1541204 531
(531 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR059c [R] KOG4372 Predicted alpha/beta hydrolase 120 5e-27
At5g51180 [R] KOG4372 Predicted alpha/beta hydrolase 89 2e-17
YDL109c [R] KOG4372 Predicted alpha/beta hydrolase 83 9e-16
At1g10040 [R] KOG4372 Predicted alpha/beta hydrolase 79 1e-14
YGL144c [R] KOG4372 Predicted alpha/beta hydrolase 79 2e-14
SPAC4A8.10 [R] KOG4372 Predicted alpha/beta hydrolase 70 8e-12
At4g25770 [R] KOG4372 Predicted alpha/beta hydrolase 62 2e-09
YDR444w [R] KOG4372 Predicted alpha/beta hydrolase 58 4e-08
>YOR059c [R] KOG4372 Predicted alpha/beta hydrolase
Length = 450
Score = 120 bits (301), Expect = 5e-27
Identities = 72/224 (32%), Positives = 118/224 (52%), Gaps = 14/224 (6%)
Query: 16 HLVVLVHGLWGNRSHMNEICNYLLSLNDSSAGHKSLNEMIVVHQTHLNEGYKTYDGIDVC 75
HL VL+HGLWGN +HM + L ++ + +N+ ++ N +KT+DGI++
Sbjct: 6 HLFVLIHGLWGNYTHMESMRTIL----STTLKKEDVNDDMIYFLPKQNAMFKTFDGIEII 61
Query: 76 GIRVSKEIKDQINHYGSDHVVKFSLIGYSLGGLICRYALGVLYQAQTFKKNDIELVNFIT 135
G R E+ + I Y + K S++GYS GGL+ R+ +G + DIE FIT
Sbjct: 62 GYRTLIEVCEFIRDYKDGKITKLSVMGYSQGGLVARFMIGKMLTEFKELFEDIEPQLFIT 121
Query: 136 FCTPHVGV---LAPGNNVAVNLFNIIVPL---VLGNSGKQMFLKDKYNGYPLLYVMSSPS 189
TPH+GV G L++ + L +LG SG++MF+ + N ++
Sbjct: 122 MATPHLGVEFYNPTGIAYKSALYSALRTLGSTILGKSGREMFIANSSNN----ILVKLSQ 177
Query: 190 SVFYKALKQFKYRALYANIINDKRTAWWTSGISKNDPFFDVNER 233
+ +AL FK+R +AN+ ND+ A++T+ I+ DPF D + +
Sbjct: 178 GEYLEALSLFKWRIAFANVKNDRTVAFYTAFITDCDPFIDFDNK 221
>At5g51180 [R] KOG4372 Predicted alpha/beta hydrolase
Length = 349
Score = 89.0 bits (219), Expect = 2e-17
Identities = 74/244 (30%), Positives = 109/244 (44%), Gaps = 55/244 (22%)
Query: 5 DGSLE-------NKGAKIHLVVLVHGLWGNRSHMNEICNYLLSLNDSSAGHKSLNEMIVV 57
DGSL+ + + HLVV+VHG+ G+ + K + + + V
Sbjct: 17 DGSLDVWSCQNSDSSSADHLVVMVHGILGSTDDWKFGAEQFV---------KKMPDKVFV 67
Query: 58 HQTHLNEGYKTYDGIDVCGIRVSKEIKDQINHYGSDHVVKFSLIGYSLGGLICRYALGVL 117
H + N T DG+DV G R++ E K I K S + +SLGGL RYA+G L
Sbjct: 68 HCSEKNVSALTLDGVDVMGERLAAERKPNI--------CKISFVAHSLGGLAARYAIGKL 119
Query: 118 YQ--------------AQTFKKNDI---ELVNFITFCTPHVGVLAPGNNVAVNLF----- 155
Y+ +Q K I E +NFIT TPH+G + GN LF
Sbjct: 120 YKPANQEDVKDSVADSSQETPKGTICGLEAMNFITVATPHLGSM--GNKQVPFLFGFSSI 177
Query: 156 ----NIIVPLVLGNSGKQMFLKDKYNGYPLL---YVMSSPSSVFYKALKQFKYRALYANI 208
+I+ + +G+ +FLKD+ G P L V + F AL+ FK R Y+N+
Sbjct: 178 EKVAGLIIHWIFKRTGRHLFLKDEEEGKPPLLRRMVEDTDDCHFISALRAFKRRVAYSNV 237
Query: 209 INDK 212
+D+
Sbjct: 238 GHDR 241
>YDL109c [R] KOG4372 Predicted alpha/beta hydrolase
Length = 647
Score = 83.2 bits (204), Expect = 9e-16
Identities = 64/209 (30%), Positives = 105/209 (49%), Gaps = 14/209 (6%)
Query: 14 KIHLVVLVHGLWGNRSHMNEICNYLLSLNDSSAGHKSLNEMIVVHQTHLNEGYKTYDGID 73
K HLV+L HG N S E YL+ A + NE +V+ + ++ +T GI
Sbjct: 192 KSHLVILTHGFQSNVSADME---YLME-EIYKAQMNNPNERLVI-KGYMKNACETEKGIK 246
Query: 74 VCGIRVSKEIKDQINHYGSDHVVKFSLIGYSLGGLICRYALGVLYQAQTFKKNDIELVNF 133
G+ ++ I D++ D V K S IG+SLGGL +A+ + + +E +NF
Sbjct: 247 FLGVGLANYIIDELY---DDSVGKISFIGHSLGGLTQTFAICYIKTKYPYFFKKVEPINF 303
Query: 134 ITFCTPHVGVLAPGNNVAVNLFNIIVPLVLGNSGKQMFLKD-KYNGYPLLYVMSSPSSVF 192
I+ +P +G+ N ++ ++G +G+++ LKD Y PLLY++S S +
Sbjct: 304 ISLASPLLGIATSTPNYVKMSLSM---GIIGTTGQELGLKDGNYGDKPLLYLLSEESLI- 359
Query: 193 YKALKQFKYRALYANIINDKRTAWWTSGI 221
L +FK R LYAN +ND ++S +
Sbjct: 360 -SVLARFKRRTLYANAVNDGIVPLYSSSL 387
>At1g10040 [R] KOG4372 Predicted alpha/beta hydrolase
Length = 379
Score = 79.3 bits (194), Expect = 1e-14
Identities = 78/264 (29%), Positives = 112/264 (41%), Gaps = 69/264 (26%)
Query: 16 HLVVLVHGLWGNRSHMNEICNYLLSLNDSSAGHKSLNEMIVVHQTHLNEGYKTYDGIDVC 75
HLVV+V+GL G+ + +L K + ++VH + N +T+DG+DV
Sbjct: 81 HLVVMVNGLIGSAQNWRFAAKQML---------KKYPQDLLVHCSKRNHSTQTFDGVDVM 131
Query: 76 GIRVSKEIKDQINHYGSDHVVKFSLIGYSLGGLICRYALGVLYQAQT-----FKKNDI-- 128
G R+++E K S +G+SLGGLI RYA+G LY+ ++ +DI
Sbjct: 132 GERLAEE--------------KISFVGHSLGGLIARYAIGRLYEQESREELPHNSDDIGD 177
Query: 129 --------------ELVNFITFCTPHVGVLAPGNNVAVNLFNIIVPLVLGNSGKQMFLKD 174
E V FIT TPH+G +A + LG +GK +FL D
Sbjct: 178 KCSIEEPKARIAGLEPVYFITSATPHLGSRGHKQRLATRMSG-----CLGKTGKHLFLAD 232
Query: 175 KYNGYP---LLYVMSSPSSVFYKALKQFKYRALYANIINDKRTAWWTSGISKNDPFFDVN 231
G P L V S F AL+ FK + W TS I ++ N
Sbjct: 233 SDGGKPPLLLRMVKDSRDLKFISALQCFKRHLV----------GWSTSSIRRH------N 276
Query: 232 ERNGLGRFQFVQGYEPVILNHNSP 255
E L R + Y P I+N +P
Sbjct: 277 ELPKLQRGPVNEKY-PHIVNVEAP 299
>YGL144c [R] KOG4372 Predicted alpha/beta hydrolase
Length = 685
Score = 79.0 bits (193), Expect = 2e-14
Identities = 62/220 (28%), Positives = 106/220 (48%), Gaps = 18/220 (8%)
Query: 14 KIHLVVLVHGLWGNRSHMNEICNYLLSLNDSSAGHKSLNEMIVVHQTHLNEGYKTYDGID 73
K HLVVL HGL H N + + + K+ +V + + +T G+
Sbjct: 187 KKHLVVLTHGL-----HSNVSTDLVYIMEQIYKAQKNYPHEQIVVKGYRGNVCQTEKGVK 241
Query: 74 VCGIRVSKEIKDQINHYGSDHVVKFSLIGYSLGGLICRYALGVLYQAQTFKKNDIELVNF 133
G R+++ I I + + K S +G+SLGGLI +A+ +Y+ + + +NF
Sbjct: 242 YLGTRLAEYI---IQDLYDESIRKISFVGHSLGGLIQAFAIAYIYEVYPWFFKKVNPINF 298
Query: 134 ITFCTPHVGVLAPGNNVAVNLFNIIVPLVLGNSGKQMFLK-DKYNGYPLLYVMSSPSSVF 192
IT +P +G++ L + V+G +G+ + L+ D G PLLY++S +
Sbjct: 299 ITLASPLLGIVTDNPAYIKVLLSF---GVIGKTGQDLGLENDVEVGKPLLYLLSGLPLI- 354
Query: 193 YKALKQFKYRALYANIINDKRTAWWTSGISKNDPFFDVNE 232
+ L++FK R +YAN IND +T+ + F D N+
Sbjct: 355 -EILRRFKRRTVYANAINDGIVPLYTASLL----FLDYND 389
>SPAC4A8.10 [R] KOG4372 Predicted alpha/beta hydrolase
Length = 723
Score = 70.1 bits (170), Expect = 8e-12
Identities = 72/246 (29%), Positives = 119/246 (48%), Gaps = 31/246 (12%)
Query: 16 HLVVLVHGLWGNRSHMNEICNYLLS-LNDSSAGHKSLNEMIVVHQTHLNEGYKTYDGIDV 74
HLVVL HG+ S++ YL L +SS KS+ E++VV N +T G+
Sbjct: 244 HLVVLTHGM---HSNVGADMEYLKEKLIESS---KSVKELVVVRGFTGNY-CQTEKGVRW 296
Query: 75 CGIRVSKEIKDQINHYGSDHVVKFS---LIGYSLGGLICRYALGVLYQAQTFKK--NDIE 129
G R+ + + D I +GS ++S ++ +SLGGL+ YA+G ++ A+T I
Sbjct: 297 LGKRLGEWLLD-ITGWGSASFPRYSHISVVAHSLGGLVQTYAVGYVH-AKTHGAFFQAIH 354
Query: 130 LVNFITFCTPHVGVLAPGNNVAVNLFNIIVPLVLGNSGKQMFL---KDKYNGYPLLYVMS 186
V F+T TP +GV + + + +G +G+ + L P L +MS
Sbjct: 355 PVFFVTLATPWLGVAGEHPSYIGKALSYGI---IGKTGQDLSLTPLNHSIESRPFLVLMS 411
Query: 187 SPSSVFYKALKQFKYRALYANIINDKRTAWWTSGISKNDPFFDVNERNGLGRFQFVQGYE 246
PS+ F++A+ F+ R L+AN ND + TS + E + LG+ + +G +
Sbjct: 412 DPSTPFFQAVSFFEKRILFANTTNDYIVPFGTSAM----------EVSSLGKVEEAEGSD 461
Query: 247 PVILNH 252
V+ H
Sbjct: 462 KVMPTH 467
>At4g25770 [R] KOG4372 Predicted alpha/beta hydrolase
Length = 395
Score = 62.0 bits (149), Expect = 2e-09
Identities = 59/226 (26%), Positives = 93/226 (41%), Gaps = 44/226 (19%)
Query: 16 HLVVLVHGLWGNRSHMNEICN-YLLSLNDSSAGHKSLNEMIVVHQTHLNEGYKTYDGIDV 74
HLVV+V+G+ G+ + ++ D H++ + N T+DG+D
Sbjct: 93 HLVVMVNGIVGSAADWKYAAEQFVKKFPDKVLVHRACFLTYRYCGSESNSATLTFDGVDK 152
Query: 75 CGIRVSKEIK----------DQINHYGSDHVVKFSLIGYSLGGLICRYALGVLYQ----- 119
G R++ E+ + H + K S + +SLGGL+ RYA+G LY+
Sbjct: 153 MGERLANEVVFGCIVVSSVLGVVKHRSG--LKKISFVAHSLGGLVARYAIGKLYEQPGEV 210
Query: 120 -----------AQTFKKNDIELVNFITFCTPHVG-------VLAPGNNVAVNLFNIIVPL 161
A+ + +E +NFITF TPH+G + G + L
Sbjct: 211 DSLDSPSKEKSARGGEIAGLEPMNFITFATPHLGSRGHRQFPILCGLPFLERTASQTAHL 270
Query: 162 VLGNSGKQMFLKDKYNGYPLLYVMSSPSSVFYKALKQFKYRALYAN 207
G +GK +FL D ++ S + AL FK R YAN
Sbjct: 271 AAGRTGKHLFLID--------MLIISSYDLNRSALNAFKRRVAYAN 308
>YDR444w [R] KOG4372 Predicted alpha/beta hydrolase
Length = 687
Score = 57.8 bits (138), Expect = 4e-08
Identities = 59/226 (26%), Positives = 101/226 (44%), Gaps = 45/226 (19%)
Query: 15 IHLVVLVHGLWGNRSHMNEICNYLLSLND----SSAGHKSLNEMIVVHQTHLNEGYKTYD 70
+HLV++ HG++ N C+ L + + +S+N I+V N G K+
Sbjct: 196 VHLVIMTHGIFSNIG-----CDMLYMKDKIEEMTFPMDESINPNIIVRGFMGNVG-KSGH 249
Query: 71 GIDVCGIRVSKEIKDQINHYGSDHVV-KFSLIGYSLGGLICRYALGVLYQAQTFKKND-- 127
GI G+RV K + + ++ + V + S IG+SLGG A+ + + K+ D
Sbjct: 250 GIHCLGVRVGKYVLETVDKLNKKYKVDRISFIGHSLGGPTQSMAVRYI----SIKRPDFF 305
Query: 128 -----IELVNFITFCTPHVGVLAPGNNVAVNLFNIIVPL---VLGNSGKQMFLK------ 173
++ VNFIT +P +GV+ F + VPL LG +G+ + LK
Sbjct: 306 DAVKGVKPVNFITLASPFIGVIGDFP------FYLSVPLDMGALGLTGRDLNLKYTPLTS 359
Query: 174 --------DKYNGYPLLYVMSSPSSVFYKALKQFKYRALYANIIND 211
+ Y + + P + K + FK R +YAN+++D
Sbjct: 360 KDGLYADDEVYPEHSKYILEILPQAPAKKVFESFKRRTIYANVMDD 405
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.137 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,892,222
Number of Sequences: 60738
Number of extensions: 1464961
Number of successful extensions: 4031
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 4006
Number of HSP's gapped (non-prelim): 11
length of query: 531
length of database: 30,389,216
effective HSP length: 111
effective length of query: 420
effective length of database: 23,647,298
effective search space: 9931865160
effective search space used: 9931865160
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)