ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI4436 good E KOG1368 Amino acid transport and metabolism Threonine aldolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI4436 1546026 1544842 -395
(395 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YEL046c [E] KOG1368 Threonine aldolase 603 e-172
SPAC23H3.09c [E] KOG1368 Threonine aldolase 278 1e-74
At3g04520 [E] KOG1368 Threonine aldolase 173 3e-43
At1g08630 [E] KOG1368 Threonine aldolase 158 1e-38
CE23914 [E] KOG1368 Threonine aldolase 154 2e-37
CE23913 [E] KOG1368 Threonine aldolase 154 2e-37
7301016 [E] KOG1368 Threonine aldolase 145 8e-35
>YEL046c [E] KOG1368 Threonine aldolase
Length = 387
Score = 603 bits (1556), Expect = e-172
Identities = 287/380 (75%), Positives = 325/380 (85%)
Query: 16 PAYTSAANDLRSDTFTTPTKEMLEAAFNASIGDAVYNEDVDTIELETVVARLAGKEAGLF 75
P Y +AANDLRSDTFTTPT EM+EAA ASIGDAVY EDVDT+ LE VAR+AGKEAGLF
Sbjct: 8 PKYITAANDLRSDTFTTPTAEMMEAALEASIGDAVYGEDVDTVRLEQTVARMAGKEAGLF 67
Query: 76 CVSGTLSNQIGLRTHLFQPPYSILCDYRAHVYTHEAAGLAILSQAMVTPVIPSNGDYMTL 135
CVSGTLSNQI +RTHL QPPYSILCDYRAHVYTHEAAGLAILSQAMV PV+PSNGDY+TL
Sbjct: 68 CVSGTLSNQIAIRTHLMQPPYSILCDYRAHVYTHEAAGLAILSQAMVVPVVPSNGDYLTL 127
Query: 136 EDIKAHYIPDDGDIHGAPTRVLSLENTLHGIVYPLEELIRIKGWCLENDIKLHCDGARIW 195
EDIK+HY+PDDGDIHGAPTR++SLENTLHGIVYPLEEL+RIK WC+EN +KLHCDGARIW
Sbjct: 128 EDIKSHYVPDDGDIHGAPTRLISLENTLHGIVYPLEELVRIKAWCMENGLKLHCDGARIW 187
Query: 196 NAVVESGVSLKQYGEIFDSISICLSKSMGAPMGSVLVGSNKFVKKCNHFRKQQGGGVRQS 255
NA +SGV LKQYGEIFDSISICLSKSMGAP+GSVLVG+ KFVKK HFRKQQGGG+RQS
Sbjct: 188 NAAAQSGVPLKQYGEIFDSISICLSKSMGAPIGSVLVGNLKFVKKATHFRKQQGGGIRQS 247
Query: 256 GIMCRMALCAVNGDWKTKMGYSHQLAHELAKFCKDNGIPLESPADTNFVFLDLQKAKMNP 315
G+M RMAL +N DWK+++ YSH LAHELA++C+ GIPLESPADTNFVF++L+ A+M+P
Sbjct: 248 GMMARMALVNINNDWKSQLLYSHSLAHELAEYCEAKGIPLESPADTNFVFINLKAARMDP 307
Query: 316 DXXXXXXXXXXXXXXXXRVSFHYQVTRETLENVKSAVLETFQYAKENPFDTNGPTKIYRS 375
D RVSFHYQVTR+TLE VK A+ E F YAKE+PFD NGPT+IYRS
Sbjct: 308 DVLVKKGLKYNVKLMGGRVSFHYQVTRDTLEKVKLAISEAFDYAKEHPFDCNGPTQIYRS 367
Query: 376 ESTDFDIHGNPISDIKTYKY 395
EST+ D+ GN I +IKTYKY
Sbjct: 368 ESTEVDVDGNAIREIKTYKY 387
>SPAC23H3.09c [E] KOG1368 Threonine aldolase
Length = 376
Score = 278 bits (711), Expect = 1e-74
Identities = 146/335 (43%), Positives = 205/335 (60%), Gaps = 6/335 (1%)
Query: 23 NDLRSDTFTTPTKEMLEAAFNASIGDAVYNEDVDTIELETVVARLAGKEAGLFCVSGTLS 82
ND RSDT T PT EM + AS GD VY ED DT +LE VA+L GKEA LF SGT
Sbjct: 29 NDFRSDTLTVPTDEMRRIMYEASDGDCVYEEDEDTRKLEVYVAKLTGKEAALFVTSGTQG 88
Query: 83 NQIGLRTHLFQPPYSILCDYRAHVYTHEAAGLAILSQAMVTPVIPSNGDYMTLEDIKAHY 142
NQ+ +R+HL QPP+SI+CD RAH+Y EA + + +QA+V P+ P N Y+T E+I+
Sbjct: 89 NQLCIRSHLHQPPHSIICDDRAHIYNWEAGAIGLFTQAIVRPISPKNNVYITAEEIENKL 148
Query: 143 IPDDGDIHGAPTRVLSLENTLHGIVYPLEELIRIKGWCLENDIKLHCDGARIWNAVVESG 202
I + DIH +PT ++ LENT+ G V PL+E+ RI G + I LHCDGAR+W+A V S
Sbjct: 149 ILGN-DIHFSPTGLICLENTIKGAVVPLDEVARISGLAKAHKIPLHCDGARLWDAAVASN 207
Query: 203 VSLKQYGEIFDSISICLSKSMGAPMGSVLVGSNKFVKKCNHFRKQQGGGVRQSGIMCRMA 262
VS+K+Y FDS+S+CLSK + AP+GS++VG F+ K FRK GGG+RQSG++
Sbjct: 208 VSIKEYCSYFDSVSLCLSKGLAAPVGSIIVGPRDFIAKAKWFRKAYGGGLRQSGMLAAAG 267
Query: 263 LCAVNGDWKTKMGYSHQLAHELAKFCKDNGIPLESPADTNFVFLDLQKAKMNPDXXXXXX 322
L ++ ++ + H+ A E+A++ + GI LE P +N V L A +N
Sbjct: 268 LYSIQHNFPL-LKQVHKYAIEVAEYAESLGIELEVPTQSNMVTL----ANINVAILCDEA 322
Query: 323 XXXXXXXXXXRVSFHYQVTRETLENVKSAVLETFQ 357
R+ FH Q+T + +E +K+ + T +
Sbjct: 323 KKSGIILMGPRIVFHIQITPDAVEILKNVLRRTVE 357
>At3g04520 [E] KOG1368 Threonine aldolase
Length = 370
Score = 173 bits (439), Expect = 3e-43
Identities = 102/266 (38%), Positives = 149/266 (55%), Gaps = 4/266 (1%)
Query: 24 DLRSDTFTTPTKEMLEAAFNASIGDAVYNEDVDTIELETVVARLAGKEAGLFCVSGTLSN 83
DLRSDT T PT+ M A NA + D V D + LE VA +AGKEA +F SGT+ N
Sbjct: 11 DLRSDTVTKPTESMRSAMANAEVDDDVLGNDPTALRLEKEVAEIAGKEAAMFVPSGTMGN 70
Query: 84 QIGLRTHLFQPPYSILCDYRAHVYTHEAAGLAILSQAMVTPVIPSNGDYMTLEDIKAHYI 143
I + H + ++ +H++ +E G++ L V M + I+A
Sbjct: 71 LISVLVHCDERGSEVILGDDSHIHIYENGGVSSLGGVHPRTVKNEEDGTMEIGAIEAAVR 130
Query: 144 PDDGDIHGAPTRVLSLENT---LHGIVYPLEELIRIKGWCLENDIKLHCDGARIWNAVVE 200
GD+H T+++ LENT G P+E + ++ ++ +KLH DGARI+NA V
Sbjct: 131 SPKGDLHHPVTKLICLENTQANCGGRCLPIEYIDKVGELAKKHGLKLHIDGARIFNASVA 190
Query: 201 SGVSLKQYGEIFDSISICLSKSMGAPMGSVLVGSNKFVKKCNHFRKQQGGGVRQSGIMCR 260
GV +K+ + DS+SICLSK +GAP+GSV+VGS KF+ K RK GGG+RQ G++C
Sbjct: 191 LGVPVKRIVQAADSVSICLSKGIGAPVGSVIVGSKKFITKARWLRKTLGGGMRQIGVLCA 250
Query: 261 MALCAVNGDWKTKMGYSHQLAHELAK 286
AL A++ + K+ H+ A LA+
Sbjct: 251 AALVALHEN-VAKLEDDHKKARVLAE 275
>At1g08630 [E] KOG1368 Threonine aldolase
Length = 484
Score = 158 bits (400), Expect = 1e-38
Identities = 106/323 (32%), Positives = 157/323 (47%), Gaps = 6/323 (1%)
Query: 24 DLRSDTFTTPTKEMLEAAFNASIGDAVYNEDVDTIELETVVARLAGKEAGLFCVSGTLSN 83
DLRSDT T PT M EA NA + D V D LE +A++ GKEA LF SGT+ N
Sbjct: 91 DLRSDTVTRPTDAMREAMCNAEVDDDVLGYDPTARRLEEEMAKMMGKEAALFVPSGTMGN 150
Query: 84 QIGLRTHLFQPPYSILCDYRAHVYTHEAAGLAILSQAMVTPVIPSNGDYMTLEDIKAHYI 143
I + H ++ H++ +E G++ + V M LE I+A
Sbjct: 151 LISVMVHCDVRGSEVILGDNCHIHVYENGGISTIGGVHPKTVKNEEDGTMDLEAIEAAIR 210
Query: 144 PDDGDIHGAPTRVLSLENT---LHGIVYPLEELIRIKGWCLENDIKLHCDGARIWNAVVE 200
G TR++ LENT G +E ++ + +KLH DGAR++NA +
Sbjct: 211 DPKGSTFYPSTRLICLENTHANSGGRCLSVEYTEKVGEIAKRHGVKLHIDGARLFNASIA 270
Query: 201 SGVSLKQYGEIFDSISICLSKSMGAPMGSVLVGSNKFVKKCNHFRKQQGGGVRQSGIMCR 260
GV + + + DS+ +CLSK +GAP+GSV+VGS F++K RK GGG+RQ G++C
Sbjct: 271 LGVPVHKLVKAADSVQVCLSKGLGAPVGSVIVGSQSFIEKAKTVRKTLGGGMRQIGVLCA 330
Query: 261 MALCAVNGDWKTKMGYSHQLAHELAKFCKD-NGIPLESPA-DTNFVFLDLQKAKMNPDXX 318
AL A+ + K+ + H+ A LA+ GI + A +TN L K+ +
Sbjct: 331 AALVALQEN-LPKLQHDHKKAKLLAEGLNQMKGIRVNVAAVETNMDGSRLTAEKLRKNLE 389
Query: 319 XXXXXXXXXXXXXXRVSFHYQVT 341
R+ H+Q+T
Sbjct: 390 ENGILLIRGNSSRIRIVIHHQIT 412
>CE23914 [E] KOG1368 Threonine aldolase
Length = 433
Score = 154 bits (390), Expect = 2e-37
Identities = 108/310 (34%), Positives = 151/310 (47%), Gaps = 29/310 (9%)
Query: 8 STAAAPSEPAYTSAAN------DLRSDTFTTPTKEMLEAAFNASIGDAVYNEDVDTIELE 61
ST + AYT +N DLRSDT T P+ EM A A +GD VY ED T LE
Sbjct: 46 STDLLDTPSAYTQKSNKTHTSIDLRSDTVTVPSVEMRRAMAEAIVGDDVYGEDTTTNRLE 105
Query: 62 TVVARLAGKEAGLFCVSGTLSNQIGLRTHLFQPPYSILCDYRAHVYTHEAAGLAILSQAM 121
A L GKEAGLF SGT+ N + + H + I+ Y H++ E A +
Sbjct: 106 QRCAELFGKEAGLFVTSGTMGNLLAIMAHCQRGEEIIVGRYN-HIHRWEQGNYAQFAGIS 164
Query: 122 VTPVIPSNGDYMTLEDI------KAHYIP------DD------GDIHGAPTRVLSLENTL 163
T + M L DI K Y+ DD D H ++++ +ENT
Sbjct: 165 ATTLEVKPDGTMDLNDIEQAIRVKGGYVQIKDESCDDLEVTSIPDCHMPASKLICIENTH 224
Query: 164 H---GIVYPLEELIRIKGWCLENDIKLHCDGARIWNAVVESGVSLKQYGEIFDSISICLS 220
+ G P+E + +K D+K+H DGARI+NA V S S+ + D++ +C S
Sbjct: 225 NYTGGKALPIEWMRSVKQLAERRDLKVHMDGARIYNAAVASNCSVSKIASFADTVQMCFS 284
Query: 221 KSMGAPMGSVLVGSNKFVKKCNHFRKQQGGGVRQSGIMCRMALCAVNGDWKTKMGYSHQL 280
K +GAP+GS++VG F+ + H RK GGG RQSGI+ A A++ T + H+
Sbjct: 285 KGLGAPVGSIVVGPKDFIDRARHSRKALGGGWRQSGILAAAAHIALDHADAT-IRADHER 343
Query: 281 AHELAKFCKD 290
A LA+ D
Sbjct: 344 AKTLARMIND 353
>CE23913 [E] KOG1368 Threonine aldolase
Length = 428
Score = 154 bits (390), Expect = 2e-37
Identities = 108/310 (34%), Positives = 151/310 (47%), Gaps = 29/310 (9%)
Query: 8 STAAAPSEPAYTSAAN------DLRSDTFTTPTKEMLEAAFNASIGDAVYNEDVDTIELE 61
ST + AYT +N DLRSDT T P+ EM A A +GD VY ED T LE
Sbjct: 41 STDLLDTPSAYTQKSNKTHTSIDLRSDTVTVPSVEMRRAMAEAIVGDDVYGEDTTTNRLE 100
Query: 62 TVVARLAGKEAGLFCVSGTLSNQIGLRTHLFQPPYSILCDYRAHVYTHEAAGLAILSQAM 121
A L GKEAGLF SGT+ N + + H + I+ Y H++ E A +
Sbjct: 101 QRCAELFGKEAGLFVTSGTMGNLLAIMAHCQRGEEIIVGRYN-HIHRWEQGNYAQFAGIS 159
Query: 122 VTPVIPSNGDYMTLEDI------KAHYIP------DD------GDIHGAPTRVLSLENTL 163
T + M L DI K Y+ DD D H ++++ +ENT
Sbjct: 160 ATTLEVKPDGTMDLNDIEQAIRVKGGYVQIKDESCDDLEVTSIPDCHMPASKLICIENTH 219
Query: 164 H---GIVYPLEELIRIKGWCLENDIKLHCDGARIWNAVVESGVSLKQYGEIFDSISICLS 220
+ G P+E + +K D+K+H DGARI+NA V S S+ + D++ +C S
Sbjct: 220 NYTGGKALPIEWMRSVKQLAERRDLKVHMDGARIYNAAVASNCSVSKIASFADTVQMCFS 279
Query: 221 KSMGAPMGSVLVGSNKFVKKCNHFRKQQGGGVRQSGIMCRMALCAVNGDWKTKMGYSHQL 280
K +GAP+GS++VG F+ + H RK GGG RQSGI+ A A++ T + H+
Sbjct: 280 KGLGAPVGSIVVGPKDFIDRARHSRKALGGGWRQSGILAAAAHIALDHADAT-IRADHER 338
Query: 281 AHELAKFCKD 290
A LA+ D
Sbjct: 339 AKTLARMIND 348
>7301016 [E] KOG1368 Threonine aldolase
Length = 383
Score = 145 bits (367), Expect = 8e-35
Identities = 93/259 (35%), Positives = 136/259 (51%), Gaps = 12/259 (4%)
Query: 19 TSAANDLRSDTFTTPTKEMLEAAFNASIGDAVYNEDVDTIELETVVARLAGKEAGLFCVS 78
T + DLRSDT + PT +M A +GD VY ED ELE A + GKEAGLF S
Sbjct: 3 THSVVDLRSDTVSQPTDKMRARMATAVVGDDVYGEDPTVNELEARTAAIFGKEAGLFVPS 62
Query: 79 GTLSNQIGLRTHLFQPPYSILCDYRAHVYTHEAAGLAILSQAMVTPVIPSNGDYMTLEDI 138
GT+ N + + H + L +H++ +E G + L+ + + +L+D+
Sbjct: 63 GTMGNLLAIMVHCHRRGTEALVGDLSHIFLYEQGGASHLAGVQLATLKNEQDGTFSLQDL 122
Query: 139 KAHYIPDDGDIHGAPTRVLSLENTLH---GIVYPLE------ELIRIKGWCLEND-IKLH 188
+ +D H T ++ +E T + G V PL L+R G N I LH
Sbjct: 123 RRRIRHED--CHEPITSLVVVEQTHNICGGKVVPLAFLDELAALVRQPGVGTGNARIALH 180
Query: 189 CDGARIWNAVVESGVSLKQYGEIFDSISICLSKSMGAPMGSVLVGSNKFVKKCNHFRKQQ 248
DGAR++NA GV +++ FDS+SICLSK + AP+GSVLVGS F+ + + RK
Sbjct: 181 MDGARVFNAATALGVGVERICRDFDSVSICLSKGLSAPVGSVLVGSKAFISEAHRLRKAL 240
Query: 249 GGGVRQSGIMCRMALCAVN 267
GGG+RQ G++ L A++
Sbjct: 241 GGGMRQVGVLAAAGLVALD 259
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.134 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,626,547
Number of Sequences: 60738
Number of extensions: 985967
Number of successful extensions: 2158
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2143
Number of HSP's gapped (non-prelim): 7
length of query: 395
length of database: 30,389,216
effective HSP length: 108
effective length of query: 287
effective length of database: 23,829,512
effective search space: 6839069944
effective search space used: 6839069944
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)