ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactVI4516   suspect: LH   Z     KOG2659  Cytoskeleton                                                   LisH motif-containing protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI4516 1571290  1572348 353  
         (353 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YMR135c [Z] KOG2659 LisH motif-containing protein                     224   1e-58
SPAC12B10.13 [Z] KOG2659 LisH motif-containing protein                 57   5e-08

>YMR135c [Z] KOG2659 LisH motif-containing protein
          Length = 455

 Score =  224 bits (571), Expect = 1e-58
 Identities = 161/428 (37%), Positives = 213/428 (49%), Gaps = 85/428 (19%)

Query: 7   TYEKKSFTREQWMKVVQD--------ANPYDRSNSELSNN------------------EP 40
           TY KK FT+E+W + V          AN       ++  N                  EP
Sbjct: 28  TYGKKCFTKEEWKEQVAKYSAMGELYANKTIHYPLKIQPNSSGGSQDEGFATIQTTPIEP 87

Query: 41  VIPMLLLNYFVVMAYEESSIRMAKELGFLNSNKDIEEFNSVYMIKKRAYIKELIKKGEIL 100
            +P LLLNYFV MAYE+SSIRMAKELGF+ +NKDI  FN +Y IK+R +IK LIK G I 
Sbjct: 88  TLPRLLLNYFVSMAYEDSSIRMAKELGFIRNNKDIAVFNDLYKIKERFHIKHLIKLGRIN 147

Query: 101 LAMEKITEVFGIEVLESL-------------------NDHITDEDXXXXXXXXXXIEMIR 141
            AME+I  +FG+EVLE                          D D          IEMIR
Sbjct: 148 EAMEEINSIFGLEVLEETFNATGSYTGRTDRQQQQQQQQFDIDGDLHFKLLLLNLIEMIR 207

Query: 142 SHNAKGD-PNDQEQFILELISYAQDKLALKASSKKEYMKEVELVMTLLLFPLSDEDGXXX 200
           SH+ + +   D   FIL LI Y+Q+KLA+KASS  + M+E+EL MTLLLFPLSD      
Sbjct: 208 SHHQQENITKDSNDFILNLIQYSQNKLAIKASSSVKKMQELELAMTLLLFPLSD-SADSG 266

Query: 201 XXXXXXXXXQLYSLNMRTKVADLVNRKLLQSILPRINSQL--EKSGLPDILGSS------ 252
                     LYS+++R+K+ADLVN KLL+ I PRI  ++    S  PD+L S       
Sbjct: 267 SIKLPKSLQNLYSISLRSKIADLVNEKLLKFIHPRIQFEISNNNSKFPDLLNSDKKIITQ 326

Query: 253 ----FQSNRV-----PTLTNVIRSIP-RESITTERLTGELAHIEEKQ-------GSSMST 295
               + +N V       +T++    P  E +++  +T     +   Q       GS+ +T
Sbjct: 327 NFTVYNNNLVNGSNGTKITHISSDQPINEKMSSNEVTAAANSVWLNQRDGNVGTGSAATT 386

Query: 296 ----------NSDMSRIQGEIFEELGENLHSYY---IPNDVRLVQIMKLWVWCENQLHSS 342
                     N     +     +E G   ++YY    P + RL QIMKLW WCENQLH +
Sbjct: 387 FHNLENKNYWNQTSELLSSSNGKEKGLEFNNYYSSEFPYEPRLTQIMKLWCWCENQLHHN 446

Query: 343 DVGVPRVE 350
            +GVPRVE
Sbjct: 447 QIGVPRVE 454


>SPAC12B10.13 [Z] KOG2659 LisH motif-containing protein
          Length = 240

 Score = 56.6 bits (135), Expect = 5e-08
 Identities = 51/208 (24%), Positives = 96/208 (45%), Gaps = 25/208 (12%)

Query: 24  DANPYDRSNSELSNNEPVIPMLLLNYFVVMAYEESSIRMAKELGFLNSNKDIEEFNSVYM 83
           DA  +++    +  +   +  L+L+Y V+   EE++   A+E         I ++     
Sbjct: 18  DAEQWEKQTKSVHIDNSDVNSLILDYLVIQGDEEAAKTFAEE-------AQITDYYIPPY 70

Query: 84  IKKRAYIKELIKKGEILLAMEKITEVFGIEVLESLNDHITDEDXXXXXXXXXXIEMIRSH 143
           +K+R  I ELIK G I  A+ K+ E+   E+L+      T+ +          +E+IR  
Sbjct: 71  VKERLEICELIKSGSINSAICKLNEL-EPEILD------TNSELLFELLRLRLLELIREV 123

Query: 144 NAKGDPNDQEQFILELISYAQDKLALKASSKKEYMKEVELVMTLLLFPLSDEDGXXXXXX 203
             + D +D    +   +++A + LA  A S ++++  +EL M+LL FP S          
Sbjct: 124 VEEKDTSDLA--VERCLNFAHENLAPLAPSNQKFLNSLELTMSLLCFPPS---------S 172

Query: 204 XXXXXXQLYSLNMRTKVADLVNRKLLQS 231
                  + + + R +VA+L N  +L+S
Sbjct: 173 YSPALKNVLNYSQRERVANLANVSILKS 200


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.315    0.133    0.361 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,400,639
Number of Sequences: 60738
Number of extensions: 707976
Number of successful extensions: 1528
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1522
Number of HSP's gapped (non-prelim): 4
length of query: 353
length of database: 30,389,216
effective HSP length: 107
effective length of query: 246
effective length of database: 23,890,250
effective search space: 5877001500
effective search space used: 5877001500
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)