ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI4554 good R KOG3085 General function prediction only Predicted hydrolase (HAD superfamily)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI4554 1585069 1584149 -307
(307 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YMR130w [R] KOG3085 Predicted hydrolase (HAD superfamily) 275 5e-74
SPAC7D4.05 [R] KOG3085 Predicted hydrolase (HAD superfamily) 106 4e-23
7291981 [R] KOG3085 Predicted hydrolase (HAD superfamily) 97 4e-20
At5g44730 [R] KOG3085 Predicted hydrolase (HAD superfamily) 71 2e-12
Hs13654294 [R] KOG3085 Predicted hydrolase (HAD superfamily) 68 1e-11
At1g14310 [R] KOG3085 Predicted hydrolase (HAD superfamily) 68 2e-11
7290501 [R] KOG3085 Predicted hydrolase (HAD superfamily) 63 5e-10
At2g41250 [R] KOG3085 Predicted hydrolase (HAD superfamily) 57 4e-08
CE26719 [R] KOG3085 Predicted hydrolase (HAD superfamily) 56 7e-08
>YMR130w [R] KOG3085 Predicted hydrolase (HAD superfamily)
Length = 302
Score = 275 bits (704), Expect = 5e-74
Identities = 140/285 (49%), Positives = 190/285 (66%), Gaps = 3/285 (1%)
Query: 19 SRPKIITFDAYNTLYSIKKPVMEQYCIVGAKYGINGNPQELTKRFPGVFSNIRKKYPLYG 78
+RP IITFDAYNTLY+ K PVMEQYCIVG KYGI NP LT FP VF +++ YP YG
Sbjct: 19 ARPLIITFDAYNTLYATKLPVMEQYCIVGRKYGIKANPSTLTNNFPHVFKKLKEDYPQYG 78
Query: 79 KNSGITAEQWWEYLIRDMFEPIQIPNEMVEEILERFEGDAAYTVYPDVREFLETMRRNHP 138
K SGI EQWW LIR++F P +IP+EM+ EIL RFEG +Y VYPD+ +FL+ ++ HP
Sbjct: 79 KYSGIKPEQWWSILIRNVFAPNEIPDEMINEILMRFEGFDSYFVYPDLIKFLKDLKSRHP 138
Query: 139 EVSLGIVSNTDPIVLTLLENLDLKKYFDGNIYLSYDLEIKKPDPAMFNYAVSHMLKRHNT 198
+V LGIVSNTDPI LL+N+ L + F G+IYLSY+L + KPD A+F YA+ ++ +
Sbjct: 139 DVILGIVSNTDPIFYKLLKNIGLFETFSGHIYLSYELNLAKPDRAIFQYALDDIISKQPH 198
Query: 199 SGQRENLENIRPHVWHVGDEEKTDLGGAFQAGVNGILVDRSNSFGYFDITTPDVCKKELS 258
++ E I H +H+GDE K DL GA AG GIL+DR++ +G+ + + K
Sbjct: 199 LLEKYTREEILQHCFHIGDELKNDLEGAEAAGWTGILLDRNDKYGFL---SNSISKPMRD 255
Query: 259 EDDLSLRKVLHHSEVILQESDAVTDVVQLNSRQYVLPNFKSLEAL 303
E LS+ K+ ++S + + TD +QL+ R+YV+ N + LE L
Sbjct: 256 EYKLSIDKIDNNSINTWEANTKQTDTLQLSERKYVVSNLEVLEEL 300
>SPAC7D4.05 [R] KOG3085 Predicted hydrolase (HAD superfamily)
Length = 225
Score = 106 bits (265), Expect = 4e-23
Identities = 70/227 (30%), Positives = 111/227 (48%), Gaps = 16/227 (7%)
Query: 22 KIITFDAYNTLYSIKKPVMEQYCIVGAKYGINGNPQELTKRFPGVFSNIRKKYPLYGKNS 81
K++TFDA+ T+ + KPV Y V KYG++ E+ F + +K+ +GK S
Sbjct: 11 KLVTFDAFGTILHLSKPVPIVYSEVAQKYGVHATIDEIEH---NTFKDFSEKHKNHGKKS 67
Query: 82 GITAEQWWEYLIRDMFEPIQIPNEMVEEILERFEGDAAYTVYPDVREFLETMRRNHPEVS 141
G+ WW +I F P +P EM EE+ F YT++P + +FL+ + +
Sbjct: 68 GLNPHDWWIKVIEHSF-PTPVPAEMAEELWSYFSKKTGYTIHPLLIDFLKRNKEERKYI- 125
Query: 142 LGIVSNTDPIVLTLLENLDLKKYFDGNIY-LSYDLEIKKPDPAMFNYAVSHMLKRHNTSG 200
+GI+SNTD + T+LE+ + D IY SYD+ +KP +F+YA+ +K
Sbjct: 126 IGIISNTDERIRTVLEDYGIDHLID--IYAFSYDVGFEKPSREIFDYAMEKAVKLLGQEI 183
Query: 201 QRENLENIRPHVWHVGDEEKTDLGGAFQAGVNGILVD-RSNSFGYFD 246
Q E H+GD+ D+ A N D ++N YF+
Sbjct: 184 QPE-------ECMHLGDDLIKDVSAARNIQWNAEYCDIKTNFLKYFE 223
>7291981 [R] KOG3085 Predicted hydrolase (HAD superfamily)
Length = 260
Score = 96.7 bits (239), Expect = 4e-20
Identities = 72/237 (30%), Positives = 117/237 (48%), Gaps = 24/237 (10%)
Query: 19 SRPKIITFDAYNTLYSIKKPVMEQYCIVGAKYGINGNPQELTKRFPGVFSNIRKKYPLYG 78
SR ++ITFD NTL + +QY +GA +G + EL K F + + + YP +G
Sbjct: 5 SRFRLITFDVTNTLLQFRTTPGKQYGEIGALFGARCDNNELAKNFKANWYKMNRDYPNFG 64
Query: 79 K--NSGITAEQWWEYLIRDMF--EPIQIPNEMV----EEILERFEGDAAYTVYPDVREFL 130
+ N + +QWW LI F IP+E + ++E ++ + E L
Sbjct: 65 RDTNPQMEWQQWWRKLIAGTFAESGAAIPDEKLHNFSNHLIELYKTSICWQPCNGSVELL 124
Query: 131 ETMRRN-HPE-VSLGIVSNTDPIVLTLLENLDLKKYFDGNIYLSYDLEIKKPDPAMFNYA 188
+ +R+ PE LG+++N DP + TLL+N L +Y D I SY+++ +KPDP +F A
Sbjct: 125 QQLRKELKPEKCKLGVIANFDPRLPTLLQNTKLDQYLDFAIN-SYEVQAEKPDPQIFQKA 183
Query: 189 VSHMLKRHNTSGQRENLENIRP-HVWHVGDEEKTDLGGAFQAGVNGILVDRSNSFGY 244
+ ++ L+N++P H+GD TD A + G + LV S+ Y
Sbjct: 184 M-----------EKSGLKNLKPEECLHIGDGPTTDYLAAKELGWHSALV-HEKSYAY 228
>At5g44730 [R] KOG3085 Predicted hydrolase (HAD superfamily)
Length = 255
Score = 71.2 bits (173), Expect = 2e-12
Identities = 72/260 (27%), Positives = 120/260 (45%), Gaps = 32/260 (12%)
Query: 19 SRPKIITFDAYNTLYSIKKPVMEQYCIVGAKYGIN-GNPQELTKRFPGVFSNIRKKYPLY 77
S+ + IT D TL + K + + YC+ G+ + + + + F ++++ +KYP +
Sbjct: 7 SKLRCITVDVTGTLIAYKGELGDYYCMAAKAIGLPCPDYKRVHEGFKLAYTDMAQKYPCF 66
Query: 78 GKNSGITAEQWWEYLIRDMF--EPIQIPNEMVEEILER----FEGDAAYTVYPDVREFLE 131
G ++ + WW+ +RD F + E E+I R F A Y+V+ D + FL
Sbjct: 67 GFHAKMPNIVWWKTCVRDSFVKAGYEYDEETFEKIFRRIYSTFGSAAPYSVFQDSQPFLR 126
Query: 132 TMRRNHPEVSLGIVSNTD-PIVLTLLENLDLKK-YFDGNIYLSYDLEIKKPDPAMFNYAV 189
RR + +G+VSN + +L + L K +D ++ + I+KPDP +F A
Sbjct: 127 WARRK--GLIVGLVSNAEYRYQEVILPSFGLSKAEWDFGVFSGIE-GIEKPDPRIFTLA- 182
Query: 190 SHMLKRHNTSGQRENLENIRP-HVWHVGDEEKTDLGGAFQAGVNGILVDRSNSFGYFD-- 246
L+R NI P V H+GD + D A G++ +LVDR + D
Sbjct: 183 ---LERAG--------NNIAPEEVLHIGDSMRKDYVPAKSIGMHALLVDRFKTEAAKDWI 231
Query: 247 ----ITTPD-VCKKELSEDD 261
I PD V ++L E D
Sbjct: 232 EAGAIVLPDLVAVQQLLESD 251
>Hs13654294 [R] KOG3085 Predicted hydrolase (HAD superfamily)
Length = 251
Score = 68.2 bits (165), Expect = 1e-11
Identities = 54/221 (24%), Positives = 94/221 (42%), Gaps = 23/221 (10%)
Query: 22 KIITFDAYNTLYSIKKPVMEQYCIVGAKYGINGNPQELTKRFPGVFSNIRKKYPLYGKNS 81
+++T+D +TL ++ P+ E Y +G+ P L + F + +P YG +
Sbjct: 8 RLLTWDVKDTLLRLRHPLGEAYATKARAHGLEVEPSALEQGFRQAYRAQSHSFPNYGLSH 67
Query: 82 GITAEQWWEYLIRDMF-----EPIQIPNEMVEEILERFEGDAAYTVYPDVREFLETMRRN 136
G+T+ QWW ++ F + Q + E++ + F + V + L R
Sbjct: 68 GLTSRQWWLDVVLQTFHLAGVQDAQAVAPIAEQLYKDFSHPCTWQVLDGAEDTLRECRTR 127
Query: 137 HPEVSLGIVSNTDPIVLTLLENLDLKKYFDGNIYLSYDLEIKKPDPAMFNYAVSHMLKRH 196
+ L ++SN D + +L L L+++FD + S KPDP +F A+
Sbjct: 128 --GLRLAVISNFDRRLEGILGGLGLREHFD-FVLTSEAAGWPKPDPRIFQEAL------- 177
Query: 197 NTSGQRENLENIRPHV-WHVGDEEKTDLGGAFQAGVNGILV 236
L ++ P V HVGD D G G++ LV
Sbjct: 178 -------RLAHMEPVVAAHVGDNYLCDYQGPRAVGMHSFLV 211
>At1g14310 [R] KOG3085 Predicted hydrolase (HAD superfamily)
Length = 265
Score = 67.8 bits (164), Expect = 2e-11
Identities = 53/208 (25%), Positives = 99/208 (47%), Gaps = 11/208 (5%)
Query: 24 ITFDAYNTLYSIKKPVMEQYCIVGAKYGINGNPQELTKRFPGVFSNIRKKYPLYGKNSGI 83
+ DA TL + KPV E Y +G KYG+ P E+ + F VFS +P + G
Sbjct: 45 LLLDAGGTLLQLSKPVHETYASLGQKYGLKTTPAEIKEGFKRVFS---APWPEKLRYQG- 100
Query: 84 TAEQWWEYLIRDMFEPIQIPNEMVEEILERFEGDAAYTVYPDVREFLETMRRNHPEVSLG 143
+W+ ++ + N+ E++ + + A+ + E + ++ V +
Sbjct: 101 DGRPFWKLVVSEATGCSD--NDYFEDVYQYYANGEAWHLPEGAYETMSLLK--DAGVKMA 156
Query: 144 IVSNTDPIVLTLLENLDLKKYFDGNIYLSYDLEIKKPDPAMFNYAVSHMLKRHNTSGQRE 203
+VSN D + LL++L++ FD + +S ++ +KPD +F A+ + + ++
Sbjct: 157 VVSNFDTRLRKLLKDLNVIDMFDA-VIVSAEVGYEKPDERIFKSALGIGILHSKLNAEQI 215
Query: 204 NLENIRPHVWHVGDEEKTDLGGAFQAGV 231
+++ R HVGD+E D GGA G+
Sbjct: 216 SVDVNR--AVHVGDDEGADKGGANAIGI 241
>7290501 [R] KOG3085 Predicted hydrolase (HAD superfamily)
Length = 246
Score = 63.2 bits (152), Expect = 5e-10
Identities = 54/230 (23%), Positives = 109/230 (46%), Gaps = 25/230 (10%)
Query: 20 RPKIITFDAYNTLYSIKKPVMEQYCIVGAKYGING-NPQELTKRFPGVFSNIRKKYPLYG 78
R +++TFD +TL ++ P + QY ++G+ G + + L + F F + ++P +G
Sbjct: 13 RFRLVTFDVTDTLLRLEDP-LRQYHQTAEEFGVTGVDRRRLEQCFRQQFKAMSSEHPNFG 71
Query: 79 KNS-GITAEQWWEYLIRDMFEPIQ--IPNEMVEEILER----FEGDAAYTVYPDVREFLE 131
+ S G+ ++WW L+ F + + E +E+I +R F A ++ +E ++
Sbjct: 72 RYSPGLDWQRWWLQLVARTFSCVDHGLAPEKLEKIGQRLISVFRTSACWSHVNGAQELVQ 131
Query: 132 TMRRNHPEVSLGIVSNTDPIVLTLLENLDLKKYFDGNIYLSYDLEIKKPDPAMFNYAVSH 191
+R + +GI+SN D + +L+ + FD I SY+ + KP+ +F +
Sbjct: 132 NVR--NAGKCVGIISNFDSSLPQVLDAMGFAGKFDF-ILTSYEAGVMKPERGIFEIPL-- 186
Query: 192 MLKRHNTSGQRENLENIRPHVWHVGDEEKTDLGGAFQAGVNGILVDRSNS 241
+ L+ H+G++ D GA G +G+LV +++
Sbjct: 187 -----------QRLQIPAEQALHIGNKLDMDYEGARNCGWSGLLVSNADN 225
>At2g41250 [R] KOG3085 Predicted hydrolase (HAD superfamily)
Length = 290
Score = 56.6 bits (135), Expect = 4e-08
Identities = 57/223 (25%), Positives = 96/223 (42%), Gaps = 25/223 (11%)
Query: 14 GSLQHSRPKIITFDAYNTLYSIKKPVMEQYCIVGAKYGINGNPQELTKRFPGVFSNIRKK 73
G + H K + DA TL +P + Y +G KYG+ + E+ R+ +
Sbjct: 73 GEITH---KALLVDAVGTLLVPAQPTAQIYKNIGEKYGVEYSEAEILTRYRRAYQKPWGG 129
Query: 74 YPLYGKNSGITAEQWWEYLIRDMFEPIQIPNEMVEEILERFEGDAAYTVY-PDVREFLET 132
L N A +W+Y++ ++ EE+ F + A+ + PD + +
Sbjct: 130 SHLRYVND---ARPFWQYIVTASTGCSD--SQYFEELYSYFTTEQAWKLCDPDAGKVFKA 184
Query: 133 MRRNHPEVSLGIVSNTDPIVLTLLENLDLKKYFDGNIYLSYDLEIKKPDPAMFNYAVSHM 192
++ V + IVSN D + LL L + +FD + +S ++E +KP+P +F A
Sbjct: 185 IKE--AGVKVAIVSNFDTRLRPLLRALRCEDWFDA-VAVSAEVEAEKPNPTIFLKAC--- 238
Query: 193 LKRHNTSGQRENLENIRPHVWHVGDEEKTDLGGAFQAGVNGIL 235
E LE HVGD+ + D+ GA AG + L
Sbjct: 239 ----------ELLEVNPEDAVHVGDDRRNDVWGARDAGCDAWL 271
>CE26719 [R] KOG3085 Predicted hydrolase (HAD superfamily)
Length = 248
Score = 55.8 bits (133), Expect = 7e-08
Identities = 60/246 (24%), Positives = 100/246 (40%), Gaps = 29/246 (11%)
Query: 22 KIITFDAYNTLYSIKKPVMEQYCIVGAKYGINGNPQELTKRFPGVFSNIRKKYPLYGKNS 81
K+++ DA +TL ++K+ Y +Y + + ++ F + + P +G N
Sbjct: 20 KVLSLDARDTLITMKESPPIVYSRFARQYDLEVDSDQIMGSFLKNYKRMSIASPCFGFN- 78
Query: 82 GITAEQWWEYLIRDMFEPIQIPNEM--VEEILERFEGDAAYT-----VYPDVREFLETMR 134
GI + WW ++ +E VE I A V D R+ L+ +R
Sbjct: 79 GIGNKSWWIEVVSSTLLDCAPDSEKGRVEVIAGALYNHYATPEPWKLVESDTRQTLQKLR 138
Query: 135 RNHPEVSLGIVSNTDPIVLTLLENLDLKKYFDGNIYLSYDLEIKKPDPAMFNYAVSHMLK 194
+ L ++SN D + +LL +L F + LS ++ +KPD +F V+H
Sbjct: 139 LKG--IILVVISNFDSRLKSLLSQFNLLDLFSMTV-LSGEIGYEKPDEKIFQLVVNHF-- 193
Query: 195 RHNTSGQRENLENIRP-HVWHVGDEEKTDLGGAFQAGVNGILVDRSNSFGYFDITTPDVC 253
+ I P + H+GD K D GA G +L D S+S P C
Sbjct: 194 -----------DLISPSEILHIGDNLKNDFHGAKNFGCRALLFDSSSSHN----VEPFYC 238
Query: 254 KKELSE 259
LS+
Sbjct: 239 ISRLSD 244
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.138 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,553,711
Number of Sequences: 60738
Number of extensions: 887441
Number of successful extensions: 2018
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 2004
Number of HSP's gapped (non-prelim): 9
length of query: 307
length of database: 30,389,216
effective HSP length: 106
effective length of query: 201
effective length of database: 23,950,988
effective search space: 4814148588
effective search space used: 4814148588
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)