ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI4561 check: MH R KOG0017 General function prediction only FOG: Transposon-encoded proteins with TYA, reverse transcriptase, integrase domains in various combinations
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI4561 1585317 1587146 610
(610 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR035c [R] KOG0017 FOG: Transposon-encoded proteins with TYA re... 138 2e-32
YMR167w [L] KOG1979 DNA mismatch repair protein - MLH1 family 68 4e-11
Hs4505911 [L] KOG1978 DNA mismatch repair protein - MLH2/PMS1/Pm... 59 2e-08
At4g09140 [L] KOG1979 DNA mismatch repair protein - MLH1 family 58 4e-08
SPBC1703.04 [L] KOG1979 DNA mismatch repair protein - MLH1 family 56 2e-07
Hs4557757 [L] KOG1979 DNA mismatch repair protein - MLH1 family 54 7e-07
7304079 [L] KOG1979 DNA mismatch repair protein - MLH1 family 52 2e-06
CE18978 [L] KOG1979 DNA mismatch repair protein - MLH1 family 52 3e-06
SPAC19G12.02c [L] KOG1978 DNA mismatch repair protein - MLH2/PMS... 51 4e-06
>YLR035c [R] KOG0017 FOG: Transposon-encoded proteins with TYA reverse
transcriptase integrase domains in various combinations
Length = 695
Score = 138 bits (348), Expect = 2e-32
Identities = 92/309 (29%), Positives = 164/309 (52%), Gaps = 23/309 (7%)
Query: 20 IDNPVSAVKQLIENSIDANCTKVLIKIDESSGGCRYIQLNDDGNGISVQKRSSVFVKHFK 79
I PV+AV++L++NSID+ KV I +D ++GGC YI + DDG+G+ + R S+ +++
Sbjct: 20 IYGPVAAVRELLDNSIDSGAKKVFIDVDSTTGGCEYISVKDDGSGVDIIDRPSMCLEYTT 79
Query: 80 T------DGKSCKRIGYKGRALYNIGKTASKKGSIEVITRTISENVAVKW-SPPRIRINE 132
+ D +G++G AL+ + ++KGS++V T+T + + KW + I
Sbjct: 80 SKMSSLGDISILTTLGFRGEALFLLSNLCNQKGSMQVETKTADDVIGEKWLVDSKGGITN 139
Query: 133 TIRFSKVVNPPGTTVILRDLMTGSKSSRL---AKKDKNYIKMIRDMLFHFSLIHKHICFS 189
R+ KV P GTTVILR L+ G ++ L ++ K + ++I ++ H+SLIH++I F
Sbjct: 140 GKRY-KVSCPVGTTVILRKLLGGLRARYLEISSRPRKTFDELIY-LINHYSLIHRNIRFY 197
Query: 190 CQLVNPLRDGSI------KTLEYVARFGKYEDRMSQFKKHVTKQNLIVEFEHFDEIELSE 243
LV+ ++G+I +TL+ + +++ KK V N IVE + + E
Sbjct: 198 FSLVSLQKNGAIERKQMQETLDPKISRARSLSLLARLKKPV-PLNFIVE----ENFVIDE 252
Query: 244 SLKVNIILPKIRTHGRVTELMPNLKYPCINGEAVMVQSGFGKKVNHFFKEFYKHFGKPEP 303
+ +++ILP++ V + K+ +N A+ + GK ++ Y+ F +P
Sbjct: 253 KINLDLILPRMVPESDVINIKRRFKFLSVNERALSLNLETGKTISKLLSSIYRDFSLLDP 312
Query: 304 RAWFIQFLC 312
WFI C
Sbjct: 313 MVWFINLNC 321
>YMR167w [L] KOG1979 DNA mismatch repair protein - MLH1 family
Length = 769
Score = 68.2 bits (165), Expect = 4e-11
Identities = 54/189 (28%), Positives = 97/189 (50%), Gaps = 19/189 (10%)
Query: 12 NSTSTNRVIDNPVSAVKQLIENSIDANCTKVLIKIDESSGGCRYIQLNDDGNGISVQKRS 71
N + +I +PV+A+K+++ENSIDAN T + I + E GG + +Q+ D+G+GI+ +
Sbjct: 14 NKIAAGEIIISPVNALKEMMENSIDANATMIDILVKE--GGIKVLQITDNGSGIN-KADL 70
Query: 72 SVFVKHFKT-------DGKSCKRIGYKGRALYNIGKTASKKGSIEVITRTISENVAVKWS 124
+ + F T D + G++G AL +I A + V T+ + A + S
Sbjct: 71 PILCERFTTSKLQKFEDLSQIQTYGFRGEALASISHVA----RVTVTTKVKEDRCAWRVS 126
Query: 125 PPRIRINETIRFSKVVNPPGTTVILRDLMTGSKSSRLAKKDKN--YIKMIRDMLFHFSLI 182
++ E+ + V GTT+++ DL S A + N Y K++ D++ +++
Sbjct: 127 YAEGKMLESPK--PVAGKDGTTILVEDLFFNIPSRLRALRSHNDEYSKIL-DVVGRYAIH 183
Query: 183 HKHICFSCQ 191
K I FSC+
Sbjct: 184 SKDIGFSCK 192
>Hs4505911 [L] KOG1978 DNA mismatch repair protein - MLH2/PMS1/Pms2 family
Length = 932
Score = 58.9 bits (141), Expect = 2e-08
Identities = 50/181 (27%), Positives = 92/181 (50%), Gaps = 24/181 (13%)
Query: 15 STNRVIDNPVSAVKQLIENSIDANCTKVLIKIDESSGGCRYIQLNDDGNGISVQKRSSVF 74
S++++I + VS VK+LIENS+DA T V +K++ + G I++ D+G GI +
Sbjct: 13 SSSQIITSVVSVVKELIENSLDAGATSVDVKLE--NYGFDKIEVRDNGEGIKAVDAPVMA 70
Query: 75 VKHFKT------DGKSCKRIGYKGRALYNIGKTASKKGSIEVITRTISENVAVKW---SP 125
+K++ + D ++ G++G AL +I A + + TRT ++N + ++
Sbjct: 71 MKYYTSKINSHEDLENLTTYGFRGEALGSICCIA----EVLITTRTAADNFSTQYVLDGS 126
Query: 126 PRIRINETIRFSKVVNPPGTTV----ILRDLMTGSKSSRLAKKDKNYIKMIRDMLFHFSL 181
I + + GTTV + ++L + AKK K+ IK I+D+L F +
Sbjct: 127 GHILSQKPSHLGQ-----GTTVTALRLFKNLPVRKQFYSTAKKCKDEIKKIQDLLMSFGI 181
Query: 182 I 182
+
Sbjct: 182 L 182
>At4g09140 [L] KOG1979 DNA mismatch repair protein - MLH1 family
Length = 737
Score = 58.2 bits (139), Expect = 4e-08
Identities = 53/212 (25%), Positives = 104/212 (49%), Gaps = 28/212 (13%)
Query: 12 NSTSTNRVIDNPVSAVKQLIENSIDANCTKVLIKIDESSGGCRYIQLNDDGNGISVQKRS 71
N + VI PVSAVK+L+ENS+DA+ + + + + + GG + IQ++DDG+GI +
Sbjct: 38 NRIAAGEVIQRPVSAVKELVENSLDADSSSISVVVKD--GGLKLIQVSDDGHGIRREDLP 95
Query: 72 SVFVKHFKT------DGKSCKRIGYKGRALYNIGKTASKKGSIEVITRTISENVAVKWSP 125
+ +H + D S +G++G AL ++ A ++ IT+ V +
Sbjct: 96 ILCERHTTSKLTKFEDLFSLSSMGFRGEALASMTYVAHV--TVTTITKGQIHGYRVSYR- 152
Query: 126 PRIRINETIRFSKVVNPPGTTVILRDLMTG--SKSSRLAKKDKNYIKMIRDMLFHFSLIH 183
+ +E + V GT +++ +L ++ L +Y K++ D+L ++ +
Sbjct: 153 DGVMEHEPKACAAV---KGTQIMVENLFYNMIARRKTLQNSADDYGKIV-DLLSRMAIHY 208
Query: 184 KHICFSCQ-----------LVNPLRDGSIKTL 204
++ FSC+ +V+P R SI+++
Sbjct: 209 NNVSFSCRKHGAVKADVHSVVSPSRLDSIRSV 240
>SPBC1703.04 [L] KOG1979 DNA mismatch repair protein - MLH1 family
Length = 684
Score = 55.8 bits (133), Expect = 2e-07
Identities = 47/195 (24%), Positives = 91/195 (46%), Gaps = 20/195 (10%)
Query: 12 NSTSTNRVIDNPVSAVKQLIENSIDANCTKVLIKIDESSGGCRYIQLNDDGNGIS----- 66
N + +I+ P +A+K+LIENS+DA T + + + + GG + +Q+ D+G+GI
Sbjct: 18 NKIAAGEIIERPENAIKELIENSLDAGSTSIDVLLKD--GGLKLLQITDNGSGIQYDDLP 75
Query: 67 --VQKRSSVFVKHFKTDGKSCKRIGYKGRALYNIGKTASKKGSIEVITRTISENVA---- 120
Q+ S+ + +F D + + G++G AL +I A + V+T+ S+ A
Sbjct: 76 YLCQRFSTSKIDNF-NDLQHLQTFGFRGEALASISHVA----KVTVVTKLSSDIHAWKAF 130
Query: 121 -VKWSPPRIRINETIRFSKVVNPPGTTVILRDLMTGSKSSRLA-KKDKNYIKMIRDMLFH 178
V + I + GT + DL +S + A K + I ++
Sbjct: 131 YVDGALAPISPGMSPAPQPCAGKQGTVITAEDLFYNVRSRKSALKNGSEEFRRIMILVQK 190
Query: 179 FSLIHKHICFSCQLV 193
+++ + + F+C+ V
Sbjct: 191 YAIHNDQVSFNCKKV 205
>Hs4557757 [L] KOG1979 DNA mismatch repair protein - MLH1 family
Length = 756
Score = 53.9 bits (128), Expect = 7e-07
Identities = 48/187 (25%), Positives = 87/187 (45%), Gaps = 19/187 (10%)
Query: 12 NSTSTNRVIDNPVSAVKQLIENSIDANCTKVLIKIDESSGGCRYIQLNDDGNGISVQKRS 71
N + VI P +A+K++IEN +DA T + + + E GG + IQ+ D+G GI ++
Sbjct: 17 NRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKE--GGLKLIQIQDNGTGIR-KEDL 73
Query: 72 SVFVKHFKT-------DGKSCKRIGYKGRALYNIGKTASKKGSIEVITRTISENVAVKWS 124
+ + F T D S G++G AL +I A + + T+T A + S
Sbjct: 74 DIVCERFTTSKLQSFEDLASISTYGFRGEALASISHVA----HVTITTKTADGKCAYRAS 129
Query: 125 PPRIRINETIRFSKVVNPPGTTVILRDLMTGSKSSRLAKKD--KNYIKMIRDMLFHFSLI 182
++ + GT + + DL + R A K+ + Y K++ +++ +S+
Sbjct: 130 YSDGKLKAPPK--PCAGNQGTQITVEDLFYNIATRRKALKNPSEEYGKIL-EVVGRYSVH 186
Query: 183 HKHICFS 189
+ I FS
Sbjct: 187 NAGISFS 193
>7304079 [L] KOG1979 DNA mismatch repair protein - MLH1 family
Length = 664
Score = 52.4 bits (124), Expect = 2e-06
Identities = 52/244 (21%), Positives = 105/244 (42%), Gaps = 39/244 (15%)
Query: 12 NSTSTNRVIDNPVSAVKQLIENSIDANCTKVLIKIDESSGGCRYIQLNDDGNGISVQKRS 71
N + +I P +A+K+L+ENS+DA T + +++ +GG + +Q+ D+G GI ++
Sbjct: 19 NRIAAGEIIQRPANALKELLENSLDAQSTHIQVQV--KAGGLKLLQIQDNGTGIR-REDL 75
Query: 72 SVFVKHFKT-------DGKSCKRIGYKGRALYNIGKTASKKGSIEVITRTISENVAVKWS 124
++ + F T D G++G AL +I A + + T+T E K
Sbjct: 76 AIVCERFTTSKLTRFEDLSQIATFGFRGEALASISHVA----HLSIQTKTAKEKCGYK-- 129
Query: 125 PPRIRINETIRFSKVVNPP-------GTTVILRDLMTGSKSSRLA-KKDKNYIKMIRDML 176
T K+ P GT + + DL R A + + + ++L
Sbjct: 130 -------ATYADGKLQGQPKPCAGNQGTIICIEDLFYNMPQRRQALRSPAEEFQRLSEVL 182
Query: 177 FHFSLIHKHICFSCQLVNPLRDGSIK-TLEYVARFGKYEDRMSQFKKHVTKQNLIVEFEH 235
+++ + + F+ + + G + L + E+ + ++K+ ++EF H
Sbjct: 183 ARYAVHNPRVGFTLR-----KQGDAQPALRTPVASSRSENIRIIYGAAISKE--LLEFSH 235
Query: 236 FDEI 239
DE+
Sbjct: 236 RDEV 239
>CE18978 [L] KOG1979 DNA mismatch repair protein - MLH1 family
Length = 779
Score = 52.0 bits (123), Expect = 3e-06
Identities = 41/186 (22%), Positives = 82/186 (44%), Gaps = 17/186 (9%)
Query: 12 NSTSTNRVIDNPVSAVKQLIENSIDANCTKVLIKIDESSGGCRYIQLNDDGNGISVQKRS 71
N + V+ P +A+K+L+ENS+DA T++++ + +GG + +Q++D+G GI ++
Sbjct: 34 NRMAAGEVLARPCNAIKELVENSLDAGATEIMVNM--QNGGLKLLQVSDNGKGIE-REDF 90
Query: 72 SVFVKHFKT-------DGKSCKRIGYKGRALYNIGKTASKKGSIEVITRTISENVAVKWS 124
++ + F T D K G++G AL ++ A + ++++ A +
Sbjct: 91 ALVCERFATSKLQKFEDLMHMKTYGFRGEALASLSHVA----KVNIVSKRADAKCA--YQ 144
Query: 125 PPRIRINETIRFSKVVNPPGTTVILRDLMTGSKSSR-LAKKDKNYIKMIRDMLFHFSLIH 183
+ T GT + DL + R KM+ D L F++
Sbjct: 145 ANFLDGKMTADTKPAAGKNGTCITATDLFYNLPTRRNKMTTHGEEAKMVNDTLLRFAIHR 204
Query: 184 KHICFS 189
+ F+
Sbjct: 205 PDVSFA 210
>SPAC19G12.02c [L] KOG1978 DNA mismatch repair protein - MLH2/PMS1/Pms2 family
Length = 794
Score = 51.2 bits (121), Expect = 4e-06
Identities = 47/164 (28%), Positives = 82/164 (49%), Gaps = 24/164 (14%)
Query: 16 TNRVIDNPVSAVKQLIENSIDANCTKVLIKIDESSGGCRYIQLNDDGNGISVQKRSSVFV 75
+ +VI + SAVK+L+ENS+D+ T + I+ + G I++ D+G+GI S+
Sbjct: 17 SGQVITDVASAVKELVENSLDSGATTIEIRF--KNYGINSIEVVDNGSGIDAGDYESIGK 74
Query: 76 KHFKT------DGKSCKRIGYKGRALYNIGKTASKKGSIEVITRTISENVAVKWSPPRIR 129
KHF + D ++ + G++G AL ++ +VI T ++N A P ++
Sbjct: 75 KHFTSKITDFEDLEALQTFGFRGEALSSLCAVG------QVIISTATQNEA----PKGVQ 124
Query: 130 IN---ETIRFSKVVNP--PGTTVILRDLMTGSKSSRLAKKDKNY 168
+N E K+ P GT+V++ DL + R +KNY
Sbjct: 125 LNLDHEGSLKDKLTIPFQRGTSVMVNDLFC-TLPVRRKLLEKNY 167
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.135 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,012,250
Number of Sequences: 60738
Number of extensions: 1601984
Number of successful extensions: 4043
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 4027
Number of HSP's gapped (non-prelim): 13
length of query: 610
length of database: 30,389,216
effective HSP length: 112
effective length of query: 498
effective length of database: 23,586,560
effective search space: 11746106880
effective search space used: 11746106880
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)