ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI4685 good U KOG0812 Intracellular trafficking, secretion, and vesicular transport SNARE protein SED5/Syntaxin 5
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI4685 1629735 1630685 317
(317 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR026c [U] KOG0812 SNARE protein SED5/Syntaxin 5 261 9e-70
SPBPI051 [U] KOG0812 SNARE protein SED5/Syntaxin 5 156 3e-38
SPBC8D2.14c [U] KOG0812 SNARE protein SED5/Syntaxin 5 156 3e-38
Hs4507293 [U] KOG0812 SNARE protein SED5/Syntaxin 5 145 6e-35
7298290 [U] KOG0812 SNARE protein SED5/Syntaxin 5 138 9e-33
CE05944 [U] KOG0812 SNARE protein SED5/Syntaxin 5 115 1e-25
At5g05760 [U] KOG0812 SNARE protein SED5/Syntaxin 5 104 2e-22
At3g24350 [U] KOG0812 SNARE protein SED5/Syntaxin 5 103 3e-22
YOR106w [U] KOG0811 SNARE protein PEP12/VAM3/Syntaxin 7/Syntaxin 17 52 1e-06
SPBC31E1.04 [U] KOG0811 SNARE protein PEP12/VAM3/Syntaxin 7/Synt... 50 4e-06
7295604 [U] KOG0809 SNARE protein TLG2/Syntaxin 16 50 4e-06
>YLR026c [U] KOG0812 SNARE protein SED5/Syntaxin 5
Length = 340
Score = 261 bits (667), Expect = 9e-70
Identities = 150/281 (53%), Positives = 183/281 (64%), Gaps = 27/281 (9%)
Query: 40 SEFQKKASEIAHEIANTAVQLGKLSQLAKRKPLLNDNPVEIMELTFLIKRRIYTIENEIM 99
SEFQKKAS IAHEI++TA L KL+ LAKRKP+ NDNPVEI EL+FLIKR+IY IE ++
Sbjct: 52 SEFQKKASGIAHEISSTAQLLSKLAVLAKRKPMFNDNPVEIAELSFLIKRKIYAIEQSLV 111
Query: 100 ELNKL---------------QIGTKQHKQNVMTLLNTKMKNISGNFKDVLETRQKLELEN 144
+L++L Q QH +NV+ LLNT+MKNISG+FKDVLE RQ+LE+ N
Sbjct: 112 QLSQLKKTDVNGNTSNQSSKQPSAVQHSKNVVNLLNTQMKNISGSFKDVLEERQRLEMAN 171
Query: 145 QDRLERLTHVGGXXXXXXXXXXXXLVPAGASSNIIGHGYNNVNPFISNLIDD--ETNNTS 202
+DR ++LT G YNN NPF+++L+D+ E NN S
Sbjct: 172 KDRWQKLTTDTGHAPADDQTQSNHAADLTT--------YNNSNPFMTSLLDESSEKNNNS 223
Query: 203 SSXXXXXXXXXXXXXXXXEEQQ--DQRYLQERSNAIETIESTIQEVGNLFQQLAHMVQEQ 260
S+ EE Q + YLQER+ A+ETIESTIQEVGNLFQQLA MVQEQ
Sbjct: 224 SNQGELSFPQNDSQLMLMEEGQLSNNVYLQERNRAVETIESTIQEVGNLFQQLASMVQEQ 283
Query: 261 GETIQRIDDNVGDIEMNIHGAQRELLKYFDNISNNRWMAVK 301
GE IQRID NV DI++NI GAQRELLKYFD I +NRW+A K
Sbjct: 284 GEVIQRIDANVDDIDLNISGAQRELLKYFDRIKSNRWLAAK 324
>SPBPI051 [U] KOG0812 SNARE protein SED5/Syntaxin 5
Length = 309
Score = 156 bits (395), Expect = 3e-38
Identities = 98/271 (36%), Positives = 146/271 (53%), Gaps = 25/271 (9%)
Query: 39 KSEFQKKASEIAHEIANTAVQLGKLSQLAKRKPLLNDNPVEIMELTFLIKRRIYTIENEI 98
KSEF + A +IA++I T +L KLSQLAKRK L +D PVEI ELTF IK+ + ++ ++I
Sbjct: 40 KSEFTRIAQKIANQINQTGEKLQKLSQLAKRKTLFDDRPVEIQELTFQIKQSLSSLNSDI 99
Query: 99 MELNKLQIGTK-------QHKQNVMTLLNTKMKNISGNFKDVLETRQKLELENQDRLERL 151
L ++ G + QH +NV+ L + N S FKD+LE R + +Q+R E+
Sbjct: 100 ASLQQVVKGNRNKPAQMNQHSENVVVSLQNSLANTSMTFKDILEIRTQNMKASQNRTEKF 159
Query: 152 THVGGXXXXXXXXXXXXLVPAGASSNIIGHGYNNVNPFIS-NLIDDETNNTSSSXXXXXX 210
+ + ++N + + N+++PF N E N S
Sbjct: 160 -----------------VASSSMNANPLINSGNSISPFADYNDPKPEANEDYLSLNLGDG 202
Query: 211 XXXXXXXXXXEEQQDQRYLQERSNAIETIESTIQEVGNLFQQLAHMVQEQGETIQRIDDN 270
E Q Y Q+R ++I+ IESTI E+G +F QLA MV EQ ET+QRID +
Sbjct: 203 ANTRYEQMALLESQTDTYSQQRMSSIQNIESTITELGGIFSQLAQMVSEQRETVQRIDMH 262
Query: 271 VGDIEMNIHGAQRELLKYFDNISNNRWMAVK 301
DI NI AQRE++K+++ +S+NR + K
Sbjct: 263 TDDIVSNIGSAQREIVKFYERMSSNRALLFK 293
>SPBC8D2.14c [U] KOG0812 SNARE protein SED5/Syntaxin 5
Length = 309
Score = 156 bits (395), Expect = 3e-38
Identities = 98/271 (36%), Positives = 146/271 (53%), Gaps = 25/271 (9%)
Query: 39 KSEFQKKASEIAHEIANTAVQLGKLSQLAKRKPLLNDNPVEIMELTFLIKRRIYTIENEI 98
KSEF + A +IA++I T +L KLSQLAKRK L +D PVEI ELTF IK+ + ++ ++I
Sbjct: 40 KSEFTRIAQKIANQINQTGEKLQKLSQLAKRKTLFDDRPVEIQELTFQIKQSLSSLNSDI 99
Query: 99 MELNKLQIGTK-------QHKQNVMTLLNTKMKNISGNFKDVLETRQKLELENQDRLERL 151
L ++ G + QH +NV+ L + N S FKD+LE R + +Q+R E+
Sbjct: 100 ASLQQVVKGNRNKPAQMNQHSENVVVSLQNSLANTSMTFKDILEIRTQNMKASQNRTEKF 159
Query: 152 THVGGXXXXXXXXXXXXLVPAGASSNIIGHGYNNVNPFIS-NLIDDETNNTSSSXXXXXX 210
+ + ++N + + N+++PF N E N S
Sbjct: 160 -----------------VASSSMNANPLINSGNSISPFADYNDPKPEANEDYLSLNLGDG 202
Query: 211 XXXXXXXXXXEEQQDQRYLQERSNAIETIESTIQEVGNLFQQLAHMVQEQGETIQRIDDN 270
E Q Y Q+R ++I+ IESTI E+G +F QLA MV EQ ET+QRID +
Sbjct: 203 ANTRYEQMALLESQTDTYSQQRMSSIQNIESTITELGGIFSQLAQMVSEQRETVQRIDMH 262
Query: 271 VGDIEMNIHGAQRELLKYFDNISNNRWMAVK 301
DI NI AQRE++K+++ +S+NR + K
Sbjct: 263 TDDIVSNIGSAQREIVKFYERMSSNRALLFK 293
>Hs4507293 [U] KOG0812 SNARE protein SED5/Syntaxin 5
Length = 301
Score = 145 bits (367), Expect = 6e-35
Identities = 96/306 (31%), Positives = 154/306 (49%), Gaps = 35/306 (11%)
Query: 6 RNRTIEFQKRCXXXXXXXXXXXXXXXXXXXXPNKSEFQKKASEIAHEIANTAVQLGKLSQ 65
R+RT EF C +SEF A I +++NT +L KL+
Sbjct: 4 RDRTQEFLSACKSLQTRQNGIQTNKPALRAVRQRSEFTLMAKRIGKDLSNTFAKLEKLTI 63
Query: 66 LAKRKPLLNDNPVEIMELTFLIKRRIYTIENEIMELNKL------QIGT--KQHKQNVMT 117
LAKRK L +D VEI ELT++IK+ I ++ +I +L Q G + H ++
Sbjct: 64 LAKRKSLFDDKAVEIEELTYIIKQDINSLNKQIAQLQDFVRAKGSQSGRHLQTHSNTIVV 123
Query: 118 LLNTKMKNISGNFKDVLETRQKLELENQDRLERLTHVGGXXXXXXXXXXXXLVP--AGAS 175
L +K+ ++S +FK VLE R + + + R E+ + L P G
Sbjct: 124 SLQSKLASMSNDFKSVLEVRTENLKQQRSRREQFSRAP--------VSALPLAPNHLGGG 175
Query: 176 SNIIGHGYNNVNPFISNLIDDETNNTSSSXXXXXXXXXXXXXXXXEEQQDQRYLQERSNA 235
+ ++G + +++D T+ ++QD Y+Q R++
Sbjct: 176 AVVLGAESHASKDVAIDMMDSRTSQQLQLI----------------DEQDS-YIQSRADT 218
Query: 236 IETIESTIQEVGNLFQQLAHMVQEQGETIQRIDDNVGDIEMNIHGAQRELLKYFDNISNN 295
++ IESTI E+G++FQQLAHMV+EQ ETIQRID+NV ++++ A E+LKYF ++++N
Sbjct: 219 MQNIESTIVELGSIFQQLAHMVKEQEETIQRIDENVLGAQLDVEAAHSEILKYFQSVTSN 278
Query: 296 RWMAVK 301
RW+ VK
Sbjct: 279 RWLMVK 284
>7298290 [U] KOG0812 SNARE protein SED5/Syntaxin 5
Length = 310
Score = 138 bits (348), Expect = 9e-33
Identities = 87/272 (31%), Positives = 133/272 (47%), Gaps = 29/272 (10%)
Query: 40 SEFQKKASEIAHEIANTAVQLGKLSQLAKRKPLLNDNPVEIMELTFLIKRRIYTIENEIM 99
SEF A I IA+T +L KL+ LAK+K L +D P EI ELT++IK + + +I
Sbjct: 41 SEFMMVARFIGKNIASTYAKLEKLTMLAKKKSLFDDRPQEIQELTYIIKGDLNALNQQIA 100
Query: 100 ELNKLQIGTKQH---------KQNVMTLLNTKMKNISGNFKDVLETRQKLELENQDRLER 150
L + ++H N++ L +K+ ++S +FK +LE R + + + R ++
Sbjct: 101 RLQDISKDQRRHTNGKHLVSHSSNMVLALQSKLASMSTDFKQILEVRTENLKQQKTRRDQ 160
Query: 151 LTHVGGXXXXXXXXXXXXLVPAGASSNIIGHGYNNVNPFISNLIDDETNNTSSSXXXXXX 210
+ G P+ A S L+ +E S
Sbjct: 161 FSQGPGPLAAHTVS------PSTAKQG-------------SLLLSEENQAVSIDMGSSDT 201
Query: 211 XXXXXXXXXXEEQQDQ-RYLQERSNAIETIESTIQEVGNLFQQLAHMVQEQGETIQRIDD 269
D Y+Q+R+ ++ IESTI E+G +FQQLAHMV+EQ E ++RID
Sbjct: 202 TPLLSTQTQMAIYDDSDNYVQQRAETMQNIESTIVELGGIFQQLAHMVKEQEEIVERIDT 261
Query: 270 NVGDIEMNIHGAQRELLKYFDNISNNRWMAVK 301
NV D E+NI A E+LKYF ++S NRW+ +K
Sbjct: 262 NVADAELNIEAAHGEILKYFQSVSKNRWLMIK 293
>CE05944 [U] KOG0812 SNARE protein SED5/Syntaxin 5
Length = 413
Score = 115 bits (287), Expect = 1e-25
Identities = 80/271 (29%), Positives = 129/271 (47%), Gaps = 30/271 (11%)
Query: 41 EFQKKASEIAHEIANTAVQLGKLSQLAKRKPLLNDNPVEIMELTFLIKRRIYTIENEI-- 98
+F + A I E++ T ++ KL++ AK+K + +I L+ ++K I + +I
Sbjct: 146 QFNQLAKRIGKELSQTCAKMEKLAEYAKKKSCYEERS-QIDHLSSIVKSDITGLNKQIGQ 204
Query: 99 -MELNKLQIGTKQ-----HKQNVMTLLNTKMKNISGNFKDVLETRQKLELENQDRLERLT 152
E +K + G + H Q V+ L +K+ N+ +++ VLE + ++R ++ +
Sbjct: 205 LQEFSKRRAGNMKNQNSGHIQLVVVGLQSKLANVGKDYQSVLEISTETMKAEKNRRDKFS 264
Query: 153 HVGGXXXXXXXXXXXXLVPAGASSNIIGHGYNNVNPFISNLIDDETNNTSSSXXXXXXXX 212
VP G S+ G S L+ D+ + SSS
Sbjct: 265 -------------SGAAVPMGLPSSSSGANVR------SKLLQDDEQHGSSSIALDMGAL 305
Query: 213 XXXXXXXXEEQQDQ--RYLQERSNAIETIESTIQEVGNLFQQLAHMVQEQGETIQRIDDN 270
+Q+D Y Q RSN + TIE +I E+G +F QLA +V EQGE I RID N
Sbjct: 306 SNMQSQQTMQQRDSSLEYAQARSNTMATIEGSISELGQIFSQLASLVSEQGEMITRIDSN 365
Query: 271 VGDIEMNIHGAQRELLKYFDNISNNRWMAVK 301
V D +NI A EL++Y NIS NRW+ ++
Sbjct: 366 VEDTALNIDMAHSELVRYLQNISKNRWLMIQ 396
>At5g05760 [U] KOG0812 SNARE protein SED5/Syntaxin 5
Length = 336
Score = 104 bits (259), Expect = 2e-22
Identities = 80/275 (29%), Positives = 129/275 (46%), Gaps = 13/275 (4%)
Query: 40 SEFQKKASEIAHEIANTAVQLGKLSQLAKRKPLLNDNPVEIMELTFLIKRRIYTIENEIM 99
SEF KKAS I I T+ ++ +L++LAK+ + ND VEI ELT LI+ I + +
Sbjct: 45 SEFNKKASRIGLGIKETSQKITRLAKLAKQSTIFNDRTVEIQELTVLIRNDITGLNMALS 104
Query: 100 ELNKLQ---IGTKQHKQN-------VMTLLNTKMKNISGNFKDVLETRQK--LELENQDR 147
+L LQ + + Q+ V L T++ + +DVL TR + EN+ +
Sbjct: 105 DLQTLQNMELADGNYSQDQVGHYTAVCDDLKTRLMGATKQLQDVLTTRSENMKAHENRKQ 164
Query: 148 LERLTHVGGXXXXXXXXXXXXLVPAGASSNIIGHGYNNVNPFISNLIDDETNNTSSSXXX 207
L + P +SSN G+ + P ++ + S
Sbjct: 165 LFSTKNAVDSPPQNNAKSVPEPPPWSSSSNPFGNLQQPLLPPLNTGAPPGSQLRRRSAIE 224
Query: 208 XXXXXXXXXXXXXEE-QQDQRYLQERSNAIETIESTIQEVGNLFQQLAHMVQEQGETIQR 266
+ + + Y Q R+ A+ ++ES I E+ +F QLA MV +QGE R
Sbjct: 225 NAPSQQMEMSLLQQTVPKQENYSQSRAVALHSVESRITELSGIFPQLATMVTQQGELAIR 284
Query: 267 IDDNVGDIEMNIHGAQRELLKYFDNISNNRWMAVK 301
IDDN+ + +N+ GA+ LL++ IS+NRW+ +K
Sbjct: 285 IDDNMDESLVNVEGARSALLQHLTRISSNRWLMMK 319
>At3g24350 [U] KOG0812 SNARE protein SED5/Syntaxin 5
Length = 377
Score = 103 bits (258), Expect = 3e-22
Identities = 82/307 (26%), Positives = 130/307 (41%), Gaps = 43/307 (14%)
Query: 38 NKSEFQKKASEIAHEIANTAVQLGKLSQ------------------------------LA 67
NKSEF K+AS I I T+ +L KL++ +A
Sbjct: 54 NKSEFNKRASHIGLAINQTSQKLSKLAKRIRMVLRSRTDLFSGVNFSSAYLSTRECVIVA 113
Query: 68 KRKPLLNDNPVEIMELTFLIKRRIYTIENEIMEL------------NKLQIGTKQHKQNV 115
KR + +D EI ELT +IK+ I + + +++L N H V
Sbjct: 114 KRTSVFDDPTQEIQELTVVIKQEISALNSALVDLQLFRSSQNDEGNNSRDRDKSTHSATV 173
Query: 116 MTLLNTKMKNISGNFKDVLETRQKLELENQDRLERLTHVGGXXXXXXXXXXXXLVPAGAS 175
+ L ++ + + FKDVL R + ++ R + + L A+
Sbjct: 174 VDDLKYRLMDTTKEFKDVLTMRTENMKVHESRRQLFSSNASKESTNPFVRQRPLAAKAAA 233
Query: 176 SNIIGHGY-NNVNPFISNLIDDETNNTSSSXXXXXXXXXXXXXXXXEEQQDQRYLQERSN 234
S + + N + S L+ + SS Y+Q R+
Sbjct: 234 SESVPLPWANGSSSSSSQLVPWKPGEGESSPLLQQSQQQQQQQQQQMVPLQDTYMQGRAE 293
Query: 235 AIETIESTIQEVGNLFQQLAHMVQEQGETIQRIDDNVGDIEMNIHGAQRELLKYFDNISN 294
A+ T+ESTI E+ ++F QLA MV +QGE RID N+ D N+ GAQ +L +Y ++IS+
Sbjct: 294 ALHTVESTIHELSSIFTQLATMVSQQGEIAIRIDQNMEDTLANVEGAQSQLARYLNSISS 353
Query: 295 NRWMAVK 301
NRW+ +K
Sbjct: 354 NRWLMMK 360
>YOR106w [U] KOG0811 SNARE protein PEP12/VAM3/Syntaxin 7/Syntaxin 17
Length = 283
Score = 51.6 bits (122), Expect = 1e-06
Identities = 23/58 (39%), Positives = 36/58 (61%)
Query: 230 QERSNAIETIESTIQEVGNLFQQLAHMVQEQGETIQRIDDNVGDIEMNIHGAQRELLK 287
QERS I I + +QEV +F QL +V+EQGE + ID+N+ + N+ A ++L +
Sbjct: 194 QERSQQIGRIHTAVQEVNAIFHQLGSLVKEQGEQVTTIDENISHLHDNMQNANKQLTR 251
>SPBC31E1.04 [U] KOG0811 SNARE protein PEP12/VAM3/Syntaxin 7/Syntaxin 17
Length = 317
Score = 50.1 bits (118), Expect = 4e-06
Identities = 28/96 (29%), Positives = 44/96 (45%), Gaps = 2/96 (2%)
Query: 192 NLIDDETNNTSSSXXXXXXXXXXXXXXXXEEQQD--QRYLQERSNAIETIESTIQEVGNL 249
+ I++E N S S Q + QR + ER IE + I E+ +
Sbjct: 133 HFIEEEERNVSLSNNSSGQRQPLTESKISNSQLEYQQRLINERQGEIENLTQGINELNEI 192
Query: 250 FQQLAHMVQEQGETIQRIDDNVGDIEMNIHGAQREL 285
F+ L+ ++ EQGE + I+ NVG+ N A R+L
Sbjct: 193 FRDLSTIINEQGELVTNIEYNVGNTSTNTKNASRQL 228
>7295604 [U] KOG0809 SNARE protein TLG2/Syntaxin 16
Length = 352
Score = 50.1 bits (118), Expect = 4e-06
Identities = 27/79 (34%), Positives = 41/79 (51%)
Query: 222 EQQDQRYLQERSNAIETIESTIQEVGNLFQQLAHMVQEQGETIQRIDDNVGDIEMNIHGA 281
E+++ R Q R + I +I ++ ++F+ L HMVQEQG + RID NV + +
Sbjct: 254 EEENTRVAQHREQEVTKIVKSIYDLNDIFKDLGHMVQEQGTVLDRIDYNVEQTQTRVSEG 313
Query: 282 QRELLKYFDNISNNRWMAV 300
R+L K NR M V
Sbjct: 314 LRQLHKAEMYQRKNRKMCV 332
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.313 0.131 0.352
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,605,180
Number of Sequences: 60738
Number of extensions: 471609
Number of successful extensions: 1475
Number of sequences better than 1.0e-05: 11
Number of HSP's better than 0.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1447
Number of HSP's gapped (non-prelim): 17
length of query: 317
length of database: 30,389,216
effective HSP length: 106
effective length of query: 211
effective length of database: 23,950,988
effective search space: 5053658468
effective search space used: 5053658468
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)