ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI4786 good P KOG1120 Inorganic ion transport and metabolism Fe-S cluster biosynthesis protein ISA1 (contains a HesB-like domain)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI4786 1664428 1665120 231
(231 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLL027w [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (cont... 192 4e-49
At2g16710 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (co... 125 6e-29
Hs13569911 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (c... 124 8e-29
CE21678 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (cont... 124 8e-29
SPCC645.03c [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (... 123 2e-28
7293114 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (cont... 118 8e-27
At2g36260 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (co... 110 2e-24
At1g10500 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (co... 79 5e-15
Hs22065958 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (c... 75 1e-13
At5g03900 [CU] KOG1119 Mitochondrial Fe-S cluster biosynthesis p... 70 2e-12
Hs20543669 [CU] KOG1119 Mitochondrial Fe-S cluster biosynthesis ... 62 9e-10
Hs22053485_1 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 ... 55 8e-08
SPBC3B9.17 [CU] KOG1119 Mitochondrial Fe-S cluster biosynthesis ... 50 3e-06
>YLL027w [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (contains a
HesB-like domain)
Length = 250
Score = 192 bits (487), Expect = 4e-49
Identities = 97/173 (56%), Positives = 114/173 (65%), Gaps = 14/173 (8%)
Query: 73 SGSGTAGAKKTWLTYSLPKNVAKKG--EPLQTQPPKVDENIQXXXXXXXXXXXX------ 124
S S A W +Y+ P K E ++ Q +DE I
Sbjct: 78 SKSSKASKSPKWSSYAFPSRETIKSHEEAIKKQNKAIDEQIAAAVSKNDCSCTEPPKKRK 137
Query: 125 ------XXXISLTPNALSHLKGLLDQPEPKLIRIGVKNRGCSGLTYDLQYITSPGKFDEV 178
I+L+P A+ HL+ LL QPEPKLIR+ +NRGCSGLTYDLQYIT PGKFDEV
Sbjct: 138 RKLRPRKALITLSPKAIKHLRALLAQPEPKLIRVSARNRGCSGLTYDLQYITEPGKFDEV 197
Query: 179 VEQDGVKVIIDSKALFSIVGSEMDWVDDKLSSRFVFKNPNSKGTCGCGESFMV 231
VEQDGVK++IDSKALFSI+GSEMDW+DDKL+S+FVFKNPNSKGTCGCGESFMV
Sbjct: 198 VEQDGVKIVIDSKALFSIIGSEMDWIDDKLASKFVFKNPNSKGTCGCGESFMV 250
>At2g16710 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (contains a
HesB-like domain)
Length = 137
Score = 125 bits (313), Expect = 6e-29
Identities = 56/103 (54%), Positives = 77/103 (74%)
Query: 128 ISLTPNALSHLKGLLDQPEPKLIRIGVKNRGCSGLTYDLQYITSPGKFDEVVEQDGVKVI 187
++LT A S + LL Q + +R+GVK RGC+GL+Y L Y GKFDE+VE+ GV+++
Sbjct: 23 LTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRIL 82
Query: 188 IDSKALFSIVGSEMDWVDDKLSSRFVFKNPNSKGTCGCGESFM 230
++ KAL ++G++MD+VDDKL S FVF NPNS+G CGCGESFM
Sbjct: 83 VEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQGQCGCGESFM 125
>Hs13569911 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (contains a
HesB-like domain)
Length = 130
Score = 124 bits (312), Expect = 8e-29
Identities = 58/105 (55%), Positives = 82/105 (77%), Gaps = 1/105 (0%)
Query: 128 ISLTPNALSHLKGLL-DQPEPKLIRIGVKNRGCSGLTYDLQYITSPGKFDEVVEQDGVKV 186
++LTP+A++ +K LL D+PE +++GV+ RGC+GL+Y L+Y + G DE V QDGV+V
Sbjct: 26 LTLTPSAVNKIKQLLKDKPEHVGVKVGVRTRGCNGLSYTLEYTKTKGDSDEEVIQDGVRV 85
Query: 187 IIDSKALFSIVGSEMDWVDDKLSSRFVFKNPNSKGTCGCGESFMV 231
I+ KA +++G+EMD+V+DKLSS FVF NPN KGTCGCGESF +
Sbjct: 86 FIEKKAQLTLLGTEMDYVEDKLSSEFVFNNPNIKGTCGCGESFNI 130
>CE21678 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (contains a
HesB-like domain)
Length = 129
Score = 124 bits (312), Expect = 8e-29
Identities = 60/105 (57%), Positives = 79/105 (75%), Gaps = 1/105 (0%)
Query: 128 ISLTPNALSHLKGLL-DQPEPKLIRIGVKNRGCSGLTYDLQYITSPGKFDEVVEQDGVKV 186
++LT A+S ++ LL Q + ++IGV+ +GC+GLTY L+Y KFDE VEQDG+KV
Sbjct: 25 LTLTNEAVSRIRVLLAQQNDANALKIGVRQKGCNGLTYTLEYAKDKQKFDEEVEQDGIKV 84
Query: 187 IIDSKALFSIVGSEMDWVDDKLSSRFVFKNPNSKGTCGCGESFMV 231
I+ KA S++GSEMD+V DKLSS FVF+NPN KGTCGCGESF +
Sbjct: 85 WIEPKAQLSLLGSEMDYVTDKLSSEFVFRNPNIKGTCGCGESFSI 129
>SPCC645.03c [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (contains a
HesB-like domain)
Length = 190
Score = 123 bits (309), Expect = 2e-28
Identities = 57/104 (54%), Positives = 74/104 (70%), Gaps = 2/104 (1%)
Query: 128 ISLTPNALSHLKGLLDQP--EPKLIRIGVKNRGCSGLTYDLQYITSPGKFDEVVEQDGVK 185
I LTP A+ HLK + + K++RIGVK +GC+G Y L+YI P KFDE+V+QDG+
Sbjct: 83 IKLTPLAVEHLKKMQSSASMKGKMLRIGVKQKGCAGQAYSLEYIEKPDKFDEIVKQDGIS 142
Query: 186 VIIDSKALFSIVGSEMDWVDDKLSSRFVFKNPNSKGTCGCGESF 229
+I+ +AL I+GS MD+ DD L SRF+F NPN K TCGCGESF
Sbjct: 143 IIVARRALLQIIGSVMDYRDDDLQSRFIFSNPNVKSTCGCGESF 186
>7293114 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (contains a
HesB-like domain)
Length = 131
Score = 118 bits (295), Expect = 8e-27
Identities = 59/104 (56%), Positives = 77/104 (73%), Gaps = 2/104 (1%)
Query: 128 ISLTPNALSHLKGLL-DQPEPKLIRIGVKNRGCSGLTYDLQYITSPG-KFDEVVEQDGVK 185
++LTP A+ +K LL D+P+ +++GV+ RGC+GL+Y L Y + K DE V QDGVK
Sbjct: 26 LTLTPAAVLRIKTLLQDKPDMVGLKVGVRQRGCNGLSYTLDYASQKADKLDEEVVQDGVK 85
Query: 186 VIIDSKALFSIVGSEMDWVDDKLSSRFVFKNPNSKGTCGCGESF 229
V ID KA S++G+EMD+V+ KLSS FVF NPN KGTCGCGESF
Sbjct: 86 VFIDKKAQLSLLGTEMDFVESKLSSEFVFNNPNIKGTCGCGESF 129
>At2g36260 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (contains a
HesB-like domain)
Length = 109
Score = 110 bits (275), Expect = 2e-24
Identities = 50/102 (49%), Positives = 73/102 (71%), Gaps = 1/102 (0%)
Query: 128 ISLTPNALSHLKGLLDQPEPKLIRIGVKNRGCSGLTYDLQYITSPGKFDEVVEQDGVKVI 187
++L+ A + ++ LL + +R+ V+ +GC+GL+Y L Y GKFDEVVE+ GVK++
Sbjct: 6 LALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKGVKIL 65
Query: 188 IDSKALFSIVGSEMDWVDDKLSSRFVFKNPNSKGTCGCGESF 229
+D KA+ ++G+EMD+VDDKL S FVF NPN+ CGCGESF
Sbjct: 66 VDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNAT-KCGCGESF 106
>At1g10500 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (contains a
HesB-like domain)
Length = 180
Score = 79.0 bits (193), Expect = 5e-15
Identities = 40/105 (38%), Positives = 61/105 (58%), Gaps = 3/105 (2%)
Query: 128 ISLTPNALSHLKGLL-DQPEPKLIRIGVKNRGCSGLTY--DLQYITSPGKFDEVVEQDGV 184
ISL+ NAL HL + ++ E +RIGVK GCSG++Y D + + D +E G
Sbjct: 72 ISLSENALKHLSKMRSERGEDLCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGF 131
Query: 185 KVIIDSKALFSIVGSEMDWVDDKLSSRFVFKNPNSKGTCGCGESF 229
++ D K++ + G ++D+ D + F F NPN+ TCGCG+SF
Sbjct: 132 TIVCDPKSMLFLFGMQLDYSDALIGGGFSFSNPNATQTCGCGKSF 176
>Hs22065958 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (contains a
HesB-like domain)
Length = 173
Score = 74.7 bits (182), Expect = 1e-13
Identities = 37/81 (45%), Positives = 59/81 (72%), Gaps = 2/81 (2%)
Query: 131 TPNALSHLKGLL-DQPEPKLIRIGVKNRGCSGLTYDLQYITSPGKFDEVVEQDGVKVIID 189
TP+A++ +K L D+PE +++GV+ RGC+GL+Y ++Y + G DEV+ QD +V I+
Sbjct: 33 TPSAVNKIKQFLKDKPELVDVKVGVRTRGCNGLSYTVEYTKTKGNSDEVI-QDEARVFIE 91
Query: 190 SKALFSIVGSEMDWVDDKLSS 210
KA + +G+EMD+V+DKLSS
Sbjct: 92 KKAQLTPLGTEMDYVEDKLSS 112
>At5g03900 [CU] KOG1119 Mitochondrial Fe-S cluster biosynthesis protein ISA2
(contains a HesB-like domain)
Length = 757
Score = 70.5 bits (171), Expect = 2e-12
Identities = 35/106 (33%), Positives = 62/106 (58%), Gaps = 2/106 (1%)
Query: 128 ISLTPNALSHLKGLLD-QPEPKLIRIGVKNRGCSGLTYDLQYITSPGKFDEVVEQDGVKV 186
+ L+ N + +K L +PE K++R+GV+ GCSG Y + D V E++GVK+
Sbjct: 650 VHLSDNCIRRMKELQSSEPEKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKL 709
Query: 187 IIDSKALFSIVGSEMDWVDDKLSSRFVFK-NPNSKGTCGCGESFMV 231
++D+ + + G+ +D+ ++ + + FV NP++ G C C SFMV
Sbjct: 710 VVDNVSYDLVKGATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMV 755
>Hs20543669 [CU] KOG1119 Mitochondrial Fe-S cluster biosynthesis protein ISA2
(contains a HesB-like domain)
Length = 154
Score = 61.6 bits (148), Expect = 9e-10
Identities = 34/105 (32%), Positives = 55/105 (52%), Gaps = 3/105 (2%)
Query: 128 ISLTPNALSHLKGLLDQPEPKLIRIGVKNRGCSGLTYDLQYITSPGKFDEVVEQDGVKVI 187
I LT + + L + + E +R+ V+ GCSG Y T D V EQ G +V+
Sbjct: 50 IRLTDSCVQRLLEITEGSE--FLRLQVEGGGCSGFQYKFSLDTVINPDDRVFEQGGARVV 107
Query: 188 IDSKALFSIVGSEMDWVDDKLSSRF-VFKNPNSKGTCGCGESFMV 231
+DS +L + G+++D+ + + S F V NP ++ C CG SF +
Sbjct: 108 VDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQAQQGCSCGSSFSI 152
>Hs22053485_1 [P] KOG1120 Fe-S cluster biosynthesis protein ISA1 (contains a
HesB-like domain)
Length = 114
Score = 55.1 bits (131), Expect = 8e-08
Identities = 34/84 (40%), Positives = 55/84 (65%), Gaps = 16/84 (19%)
Query: 128 ISLTPNALSHLKGLL-DQPEPKLIRIGVKNRGCSGLTYDLQYITSPGKFDEVVEQDGVKV 186
++LTP+A++ +K LL D+PE +GVK ++Y + G EV+ QDGV+V
Sbjct: 25 LTLTPSAVNKIKQLLKDKPE----HVGVK----------VEYTKTKGDSGEVI-QDGVRV 69
Query: 187 IIDSKALFSIVGSEMDWVDDKLSS 210
I+ KA +++G+EMD+V++KLSS
Sbjct: 70 FIEKKAKLTLLGTEMDYVEEKLSS 93
>SPBC3B9.17 [CU] KOG1119 Mitochondrial Fe-S cluster biosynthesis protein ISA2
(contains a HesB-like domain)
Length = 205
Score = 49.7 bits (117), Expect = 3e-06
Identities = 27/87 (31%), Positives = 45/87 (51%), Gaps = 1/87 (1%)
Query: 146 EPKLIRIGVKNRGCSGLTYDLQYITSPGKFDEVVEQDGVKVIIDSKALFSIVGSEMDWVD 205
E ++R+ V GC G + G D V + +V+ D+ +L I GSE+++ +
Sbjct: 118 ENSVLRVTVDGGGCHGYQVSFRMDNKIGNADTVFVRGKARVVADNISLPLISGSEIEYTN 177
Query: 206 DKLSSRF-VFKNPNSKGTCGCGESFMV 231
+ + S F + NP +K +CGC SF V
Sbjct: 178 ELIGSSFQLLNNPRAKTSCGCNVSFDV 204
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.133 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,049,911
Number of Sequences: 60738
Number of extensions: 573531
Number of successful extensions: 1213
Number of sequences better than 1.0e-05: 13
Number of HSP's better than 0.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1198
Number of HSP's gapped (non-prelim): 13
length of query: 231
length of database: 30,389,216
effective HSP length: 103
effective length of query: 128
effective length of database: 24,133,202
effective search space: 3089049856
effective search space used: 3089049856
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)