ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI4941 good I KOG2987 Lipid transport and metabolism Fatty acid desaturase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI4941 1715742 1714591 -384
(384 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
SPBC3B8.07c [I] KOG2987 Fatty acid desaturase 337 2e-92
7297049 [I] KOG2987 Fatty acid desaturase 248 1e-65
At4g04930 [I] KOG2987 Fatty acid desaturase 238 8e-63
Hs4505193 [I] KOG2987 Fatty acid desaturase 225 7e-59
Hs18583655 [I] KOG2987 Fatty acid desaturase 216 5e-56
CE17034 [I] KOG2987 Fatty acid desaturase 208 9e-54
CE28791 [I] KOG2987 Fatty acid desaturase 207 2e-53
>SPBC3B8.07c [I] KOG2987 Fatty acid desaturase
Length = 362
Score = 337 bits (863), Expect = 2e-92
Identities = 162/364 (44%), Positives = 220/364 (59%), Gaps = 32/364 (8%)
Query: 18 PSADE----DFKVLDNFYWSAHKEPHAIRRHAIIKKHPEVQRLMGHEPLTKWIIIGVVXX 73
P A+E D + + FYW+ +EPH RR AI+K HPE+ L G+EPLTKWI++GVV
Sbjct: 13 PPAEESWNADSEDVHQFYWTYTEEPHKSRRAAILKAHPEIASLNGYEPLTKWIVLGVVSL 72
Query: 74 XXXXXXXXRNTHPFTWKFFIIAYVIGATANQNIFLAIHELSHNLAFKKPVHNKLFAIFTN 133
+ +WKFF+ AY IGA NQN+FLAIHELSHNL FKK ++N+ + +F N
Sbjct: 73 QFTCAYLLSQSSLLSWKFFLTAYFIGAFCNQNLFLAIHELSHNLGFKKTLYNRAYCLFAN 132
Query: 134 TPIGIPYSASFGPYHQLHHKFLGDEIYDTDIPTRFEAVLLSNILGKSXXXXXXXXXXXXR 193
P+G P++ASF PYH HH + G + DTD+PTR E +L N+LGK+ R
Sbjct: 133 LPVGAPFAASFRPYHMEHHAYQGVDGMDTDLPTRAELILFDNVLGKAFFCTFQLLFYAFR 192
Query: 194 PMFVTRIKFTYIHLLNVLYQFMFNFIWIANFGWYSYCYFLMSSFLAGSLHPCSGHFIAEH 253
P+ V R+ FT +H N++ QF F+++ + GW + YF MSSFLAGSLHP +GHF++EH
Sbjct: 193 PLVVRRLPFTLMHFWNIIVQFSFDYLVVRYVGWRALAYFFMSSFLAGSLHPTAGHFLSEH 252
Query: 254 YLLNIEEAIIGGKLMLKLTPAERQPINVTDESNVSREDVEFRKKYALETYSYYGLLNFFT 313
Y + I G P + P LET+SYYG LNFF
Sbjct: 253 YNMTRTRLIASG-------PGKETP---------------------LETFSYYGPLNFFV 284
Query: 314 WNVGLHNEHHDFPFVAWSRLWELNSLCPEFYNHLPKHSSWCKVLYDFITHDDVVLYNRVK 373
+N G H EHHDFP+VAW+R+ ++ L PEFY+++P SWC ++Y FIT +V ++ RVK
Sbjct: 285 YNAGYHIEHHDFPYVAWTRIGKVRELAPEFYDNIPDCKSWCGIIYQFITDSNVGMWCRVK 344
Query: 374 RVNK 377
R K
Sbjct: 345 RKQK 348
>7297049 [I] KOG2987 Fatty acid desaturase
Length = 321
Score = 248 bits (633), Expect = 1e-65
Identities = 134/349 (38%), Positives = 184/349 (52%), Gaps = 46/349 (13%)
Query: 29 NFYWSAHKEPHAIRRHAIIKKHPEVQRLMGHEPLTKWIIIGVVXXXXXXXXXXRNTHPFT 88
+F W +EPHA RR I++K+P++++L GH+P KW+ +V ++ +
Sbjct: 9 DFEWVYTEEPHASRRKIILEKYPQIKKLFGHDPNFKWVAGAMVLTQILALFVVKD---LS 65
Query: 89 WKFFIIA-YVIGATANQNIFLAIHELSHNLAF--KKPVHNKLFAIFTNTPIGIPYSASFG 145
W + I+A Y G N ++ LA+HE+SHNLAF +P+HN++ N PIG+P S SF
Sbjct: 66 WSWLIVAAYCFGGIINHSLMLAVHEISHNLAFGHSRPMHNRILGFICNLPIGLPMSISFK 125
Query: 146 PYHQLHHKFLGDEIYDTDIPTRFEAVLLSNILGKSXXXXXXXXXXXXRPMFVTRIKFTYI 205
YH HH++ GDE DTDIPT EA L GK RP+ + T +
Sbjct: 126 KYHLEHHRYQGDEAIDTDIPTLLEARLFDTTFGKFLWVCLQPFFYIFRPLIINPKPPTRL 185
Query: 206 HLLNVLYQFMFNFIWIANFGWYSYCYFLMSSFLAGSLHPCSGHFIAEHYLLNIEEAIIGG 265
++N + Q FN + + GW Y L+ S LA LHP +GHFI+EHY+
Sbjct: 186 EIINTVVQLTFNALIVYFLGWKPLAYLLIGSILAMGLHPVAGHFISEHYM---------- 235
Query: 266 KLMLKLTPAERQPINVTDESNVSREDVEFRKKYALETYSYYGLLNFFTWNVGLHNEHHDF 325
F K + ETYSYYG LN+ T+NVG HNEHHDF
Sbjct: 236 ----------------------------FAKGF--ETYSYYGPLNWITFNVGYHNEHHDF 265
Query: 326 PFVAWSRLWELNSLCPEFYNHLPKHSSWCKVLYDFITHDDVVLYNRVKR 374
P V SRL E+ + EFY+ +P+H+SW +VLYDFI V Y RVKR
Sbjct: 266 PAVPGSRLPEVKRIAKEFYDTMPQHTSWTRVLYDFIMDPAVGPYARVKR 314
>At4g04930 [I] KOG2987 Fatty acid desaturase
Length = 332
Score = 238 bits (608), Expect = 8e-63
Identities = 125/347 (36%), Positives = 177/347 (50%), Gaps = 44/347 (12%)
Query: 29 NFYWSAHKEPHAIRRHAIIKKHPEVQRLMGHEPLTKWIIIGVVXXXXXXXXXXRNTHPFT 88
+F+WS EPHA RR I+ +P++++L G +P W + + H
Sbjct: 23 DFFWSYTDEPHASRRRQILSCYPQIRQLFGPDP---WAFLKITLVVILQLSTAAILHNSG 79
Query: 89 W-KFFIIAYVIGATANQNIFLAIHELSHNLAFKKPVHNKLFAIFTNTPIGIPYSASFGPY 147
W K IAY G+ N N+FLAIHELSHNLAF PV+N+ IF N PIG+P S +F Y
Sbjct: 80 WLKILSIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRCLGIFANLPIGVPMSVTFQKY 139
Query: 148 HQLHHKFLGDEIYDTDIPTRFEAVLLSNILGKSXXXXXXXXXXXXRPMFVTRIKFTYIHL 207
H HH+F G + D D+PT EA L++NI K+ RP+F+ Y
Sbjct: 140 HLEHHRFQGVDGIDMDVPTYTEAHLVTNIFAKTIWVFLQLFFYALRPIFIKPKPPGYWEF 199
Query: 208 LNVLYQFMFNFIWIANFGWYSYCYFLMSSFLAGSLHPCSGHFIAEHYLLNIEEAIIGGKL 267
+N L Q + + + FGW S+ Y ++S+F+ G +HP +GHFI+EHY+ N +
Sbjct: 200 INFLIQIVLDVSVVLFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVFNPNQ------- 252
Query: 268 MLKLTPAERQPINVTDESNVSREDVEFRKKYALETYSYYGLLNFFTWNVGLHNEHHDFPF 327
ETYSYYG LN TW+VG HNEHHDFP
Sbjct: 253 ---------------------------------ETYSYYGPLNLLTWSVGYHNEHHDFPR 279
Query: 328 VAWSRLWELNSLCPEFYNHLPKHSSWCKVLYDFITHDDVVLYNRVKR 374
+ ++L + + E+Y L + SW +V+Y +I V Y+R+KR
Sbjct: 280 IPGNKLHLVKEIAGEYYEGLESYKSWSQVIYMYIMDTTVGPYSRMKR 326
>Hs4505193 [I] KOG2987 Fatty acid desaturase
Length = 323
Score = 225 bits (574), Expect = 7e-59
Identities = 131/353 (37%), Positives = 178/353 (50%), Gaps = 46/353 (13%)
Query: 28 DNFYWSAHKEPHAIRRHAIIKKHPEVQRLMGHEPLTKWIIIGVVXXXXXXXXXXRNTHPF 87
++F W +PHA RR I+ K+PE++ LM +P WIII +V ++
Sbjct: 8 EDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLGAFYIVKD---L 64
Query: 88 TWKFFII-AYVIGATANQNIFLAIHELSHNLAFK--KPVHNKLFAIFTNTPIGIPYSASF 144
WK+ I AY G+ N ++ LAIHE++HN AF K + N+ F +F N PIGIPYS SF
Sbjct: 65 DWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISF 124
Query: 145 GPYHQLHHKFLGDEIYDTDIPTRFEAVLLSNILGKSXXXXXXXXXXXXRPMFVTRIKFTY 204
YH HH++LG + D DIPT FE K RP+F+ TY
Sbjct: 125 KRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITY 184
Query: 205 IHLLNVLYQFMFNFIWIANFGWYSYCYFLMSSFLAGSLHPCSGHFIAEHYLLNIEEAIIG 264
+ ++N + Q F+ + G S Y L +S L LHP SGHFIAEHY+
Sbjct: 185 LEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYM--------- 235
Query: 265 GKLMLKLTPAERQPINVTDESNVSREDVEFRKKYALETYSYYGLLNFFTWNVGLHNEHHD 324
F K + ETYSYYG LN T+NVG HNEHHD
Sbjct: 236 -----------------------------FLKGH--ETYSYYGPLNLLTFNVGYHNEHHD 264
Query: 325 FPFVAWSRLWELNSLCPEFYNHLPKHSSWCKVLYDFITHDDVVLYNRVKRVNK 377
FP + L + + E+Y++LP ++SW KVLYDF+ D + Y+R+KR K
Sbjct: 265 FPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDDTISPYSRMKRHQK 317
>Hs18583655 [I] KOG2987 Fatty acid desaturase
Length = 323
Score = 216 bits (550), Expect = 5e-56
Identities = 125/350 (35%), Positives = 171/350 (48%), Gaps = 46/350 (13%)
Query: 29 NFYWSAHKEPHAIRRHAIIKKHPEVQRLMGHEPLTKWIIIGVVXXXXXXXXXXRNTHPFT 88
+F W +PH RR I+ K+P ++ LM +P KW ++ +V R
Sbjct: 9 DFEWVYTDQPHTQRRKEILAKYPAIKALMRPDPRLKWAVLVLVLVQMLACWLVRG---LA 65
Query: 89 WKFFII-AYVIGATANQNIFLAIHELSHNLAFK--KPVHNKLFAIFTNTPIGIPYSASFG 145
W++ + AY G N ++ LAIH++SHN AF + N+ A+F N P+G+PY+ASF
Sbjct: 66 WRWLLFWAYAFGGCVNHSLTLAIHDISHNAAFGTGRAARNRWLAVFANLPVGVPYAASFK 125
Query: 146 PYHQLHHKFLGDEIYDTDIPTRFEAVLLSNILGKSXXXXXXXXXXXXRPMFVTRIKFTYI 205
YH HH++LG + D D+PTR E K RP+ V T +
Sbjct: 126 KYHVDHHRYLGGDGLDVDVPTRLEGWFFCTPARKLLWLVLQPFFYSLRPLCVHPKAVTRM 185
Query: 206 HLLNVLYQFMFNFIWIANFGWYSYCYFLMSSFLAGSLHPCSGHFIAEHYLLNIEEAIIGG 265
+LN L Q + A +G Y L SSFL LHP SGHF+AEHY+
Sbjct: 186 EVLNTLVQLAADLAIFALWGLKPVVYLLASSFLGLGLHPISGHFVAEHYM---------- 235
Query: 266 KLMLKLTPAERQPINVTDESNVSREDVEFRKKYALETYSYYGLLNFFTWNVGLHNEHHDF 325
F K + ETYSYYG LN+ T+NVG H EHHDF
Sbjct: 236 ----------------------------FLKGH--ETYSYYGPLNWITFNVGYHVEHHDF 265
Query: 326 PFVAWSRLWELNSLCPEFYNHLPKHSSWCKVLYDFITHDDVVLYNRVKRV 375
P + L + + PE+Y+HLP+H SW KVL+DF+ D + Y RVKRV
Sbjct: 266 PSIPGYNLPLVRKIAPEYYDHLPQHHSWVKVLWDFVFEDSLGPYARVKRV 315
>CE17034 [I] KOG2987 Fatty acid desaturase
Length = 360
Score = 208 bits (530), Expect = 9e-54
Identities = 114/360 (31%), Positives = 174/360 (47%), Gaps = 52/360 (14%)
Query: 18 PSADEDFKVLDNFYWSAHKEPHAIRRHAIIKKHPEVQRLMGHEPLTKWIIIGVVXXXXXX 77
P+ +EDF W+ +EPHA RR I+ K+P+++ L G + + +++ +V
Sbjct: 5 PAKEEDFS------WAYTEEPHASRRKEILAKYPQIKELFGQDKAFRPVVVCMVIAQIVF 58
Query: 78 XXXXRNTHPFTWKFFII-AYVIGATANQNIFLAIHELSHNLAF--KKPVHNKLFAIFTNT 134
R++ W + AY + T N ++ LA+HE+SHN AF +P+ N++F N
Sbjct: 59 AYLLRDSD---WLLILFQAYFVSGTINHSLTLAVHEISHNQAFGTNRPLANRIFGFIANL 115
Query: 135 PIGIPYSASFGPYHQLHHKFLGDEIYDTDIPTRFEAVLLSNILGKSXXXXXXXXXXXXRP 194
P+ IP S SF YH HH+ LG+++ DTD+PT FEA + LGK RP
Sbjct: 116 PMCIPMSISFKKYHLEHHRNLGEDVIDTDVPTEFEAKVFRTWLGKMIWMSLQPLFYGIRP 175
Query: 195 MFVTRIKFTYIHLLNVLYQFMFNFIWIANFGWYSYCYFLMSSFLAGSLHPCSGHFIAEHY 254
+ T + L+NV Q +F+ FG S+ + L LHPC+GHF++EHY
Sbjct: 176 FMLYPKSMTDLELINVAIQLLFSTFIYTQFGAKSFFFLFGGLVLGMGLHPCAGHFVSEHY 235
Query: 255 LLNIEEAIIGGKLMLKLTPAERQPINVTDESNVSREDVEFRKKYALETYSYYGLLNFFTW 314
+ ++ ETYSYYG +N +
Sbjct: 236 VFKKDQ----------------------------------------ETYSYYGPINMVVF 255
Query: 315 NVGLHNEHHDFPFVAWSRLWELNSLCPEFYNHLPKHSSWCKVLYDFITHDDVVLYNRVKR 374
NVG H EHHDFP++ S L ++ + PE+Y H SW ++ DFI + + L R+KR
Sbjct: 256 NVGYHVEHHDFPYITGSNLPKVREIAPEYYQDWQTHDSWVGMMADFIFNPKMTLRKRIKR 315
>CE28791 [I] KOG2987 Fatty acid desaturase
Length = 362
Score = 207 bits (527), Expect = 2e-53
Identities = 115/350 (32%), Positives = 178/350 (50%), Gaps = 46/350 (13%)
Query: 28 DNFYWSAHKEPHAIRRHAIIKKHPEVQRLMGHEPLTKWIIIGVVXXXXXXXXXXRNTHPF 87
D+F W+ ++PH RR I+KK+PEV++L G +P K+++ +V ++
Sbjct: 8 DDFIWTLTEQPHMSRREEIVKKYPEVKKLFGVDPSLKYVVSSLVIFQIFMCWLLQDAD-- 65
Query: 88 TWKFFII-AYVIGATANQNIFLAIHELSHNLAF--KKPVHNKLFAIFTNTPIGIPYSASF 144
W I+ AY G N + LAIH++SHN AF K P+ N+ F ++ N PI +P S SF
Sbjct: 66 -WILIILEAYFCGGIINHAMTLAIHDISHNTAFGNKYPLKNRFFGMWANLPIAVPISVSF 124
Query: 145 GPYHQLHHKFLGDEIYDTDIPTRFEAVLLSNILGKSXXXXXXXXXXXXRPMFVTRIKFTY 204
YH HH++LG++ DTD+PT FEA + K RP+ + + T
Sbjct: 125 KKYHVEHHRYLGEDGLDTDVPTTFEAEFFTTAPRKLLWLALQPFFYGFRPLIIYKKAPTD 184
Query: 205 IHLLNVLYQFMFNFIWIANFGWYSYCYFLMSSFLAGSLHPCSGHFIAEHYLLNIEEAIIG 264
+ +LN + Q F+ + + FG S Y L + ++ LHP +GHFI+EHY ++
Sbjct: 185 MEILNAIIQISFDLLILHFFGVKSLFYLLFGTIISMGLHPSAGHFISEHYAFKEDQ---- 240
Query: 265 GKLMLKLTPAERQPINVTDESNVSREDVEFRKKYALETYSYYGLLNFFTWNVGLHNEHHD 324
ET+SYYGL N T+NVG H EHHD
Sbjct: 241 ------------------------------------ETFSYYGLWNLCTFNVGYHVEHHD 264
Query: 325 FPFVAWSRLWELNSLCPEFYNHLPKHSSWCKVLYDFITHDDVVLYNRVKR 374
FP++ L +L ++ PE+Y +L KH+S ++L +F+ + + Y R+KR
Sbjct: 265 FPYIPGRDLPKLRAMAPEYYENLLKHTSMMQILTEFVVNPAMGPYARLKR 314
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.326 0.141 0.456
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,517,962
Number of Sequences: 60738
Number of extensions: 1010877
Number of successful extensions: 2659
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2630
Number of HSP's gapped (non-prelim): 12
length of query: 384
length of database: 30,389,216
effective HSP length: 108
effective length of query: 276
effective length of database: 23,829,512
effective search space: 6576945312
effective search space used: 6576945312
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)