ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI4996 good O KOG0710 Posttranslational modification, protein turnover, chaperones Molecular chaperone (small heat-shock protein Hsp26/Hsp42)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI4996 1733468 1734055 196
(196 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBR072w [O] KOG0710 Molecular chaperone (small heat-shock protei... 182 2e-46
YDR171w [O] KOG0710 Molecular chaperone (small heat-shock protei... 71 8e-13
SPBC3E7.02c [O] KOG0710 Molecular chaperone (small heat-shock pr... 70 2e-12
At5g12030 [O] KOG0710 Molecular chaperone (small heat-shock prot... 64 1e-10
At5g12020 [O] KOG0710 Molecular chaperone (small heat-shock prot... 61 9e-10
At4g10250 [O] KOG0710 Molecular chaperone (small heat-shock prot... 57 2e-08
At1g59860 [O] KOG0710 Molecular chaperone (small heat-shock prot... 56 3e-08
At3g46230 [O] KOG0710 Molecular chaperone (small heat-shock prot... 55 6e-08
At1g07400 [O] KOG0710 Molecular chaperone (small heat-shock prot... 55 8e-08
At1g53540 [O] KOG0710 Molecular chaperone (small heat-shock prot... 52 7e-07
At5g59720 [O] KOG0710 Molecular chaperone (small heat-shock prot... 50 2e-06
At2g29500 [O] KOG0710 Molecular chaperone (small heat-shock prot... 50 2e-06
SPCC338.06c [O] KOG0710 Molecular chaperone (small heat-shock pr... 50 2e-06
At1g54050 [O] KOG0710 Molecular chaperone (small heat-shock prot... 48 7e-06
>YBR072w [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 214
Score = 182 bits (463), Expect = 2e-46
Identities = 100/210 (47%), Positives = 130/210 (61%), Gaps = 19/210 (9%)
Query: 1 MSFNSPFLEFFDAINQEVDQFNRLLNSSGV--VAPKKIEGNYPADS---RQVAKQXXXXX 55
MSFNSPF +FFD IN EVD FNRLL G+ AP++ N PA ++VA+
Sbjct: 1 MSFNSPFFDFFDNINNEVDAFNRLLGEGGLRGYAPRRQLANTPAKDSTGKEVARPNNYAG 60
Query: 56 XXXXXWFK--DPWFADLGQNLIP-----------PLDILDHEKEYEVHVSVPGIKEKKDI 102
+ D WF D +L P P+DILDH+ YE+ V VPG+K KKDI
Sbjct: 61 ALYDPRDETLDDWF-DNDLSLFPSGFGFPRSVAVPVDILDHDNNYELKVVVPGVKSKKDI 119
Query: 103 NLEYHKDANEIVISGEIPDVTTEETKDSAKIRERRYGKFSRVVRLPDSPGIDVENIKANY 162
++EYH++ N+I++SGEIP EE+KD K++E GKF RV+ LPD PG+D +NIKA+Y
Sbjct: 120 DIEYHQNKNQILVSGEIPSTLNEESKDKVKVKESSSGKFKRVITLPDYPGVDADNIKADY 179
Query: 163 TNGVLVLNVPKLKPTEEDKAKVYKIEVGDE 192
NGVL L VPKLKP ++ K V KIEV +
Sbjct: 180 ANGVLTLTVPKLKPQKDGKNHVKKIEVSSQ 209
>YDR171w [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 375
Score = 71.2 bits (173), Expect = 8e-13
Identities = 41/109 (37%), Positives = 63/109 (57%), Gaps = 3/109 (2%)
Query: 76 PPLDILDHEKEYEVHVSVPGIKEKKDINLEYHKDANEIVISGEIPDVTTEETKDSAKIRE 135
P +++ D E Y V +++PG + +++YH ++E++I G+I D + K KI E
Sbjct: 244 PEVNVYDTEDTYVVVLALPGANSRA-FHIDYHPSSHEMLIKGKIEDRVGIDEK-FLKITE 301
Query: 136 RRYGKFSRVVRLPDSPGIDVENIKANYTNGVLVLNVPKL-KPTEEDKAK 183
+YG F R V+ P P I E IKA Y NG+L + VPK+ TE+ K K
Sbjct: 302 LKYGAFERTVKFPVLPRIKDEEIKATYNNGLLQIKVPKIVNDTEKPKPK 350
>SPBC3E7.02c [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 143
Score = 69.7 bits (169), Expect = 2e-12
Identities = 37/109 (33%), Positives = 68/109 (61%), Gaps = 6/109 (5%)
Query: 74 LIPPLDILDHEKEYEVHVSVPGIKEKKDINLEYHKDANEIVISGEIPDVT-TEETKDSAK 132
L P +D+ + + V V +PG+K K+D+ + Y D+ ++ ISGE+ + E T+ + +
Sbjct: 35 LSPSIDVHEGKDTVSVDVELPGVK-KEDVQVHY--DSGKLTISGEVVNERKNESTEGNQR 91
Query: 133 IRERRYGKFSRVVRLPDSPGIDVENIKANYTNGVLVLNVPKLKPTEEDK 181
ERR+G FSR + +P ID + I+AN++NG+L + +PK++ ++ K
Sbjct: 92 WSERRFGSFSRTITIPAK--IDADRIEANFSNGLLTVTLPKVEKSQTKK 138
>At5g12030 [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 156
Score = 63.9 bits (154), Expect = 1e-10
Identities = 40/114 (35%), Positives = 61/114 (53%), Gaps = 6/114 (5%)
Query: 77 PLDILDHEKEYEVHVSVPGIK-EKKDINLEYHKDANEIVISGEIPDVTTEETKDSAKIRE 135
P D+++H Y V +PGIK ++ + +E + N +V+SG+ E E
Sbjct: 47 PADVIEHPDAYVFAVDMPGIKGDEIQVQIE---NENVLVVSGKRQRDNKENEGVKFVRME 103
Query: 136 RRYGKFSRVVRLPDSPGIDVENIKANYTNGVLVLNVPKLKPTEEDKAKVYKIEV 189
RR GKF R +LPD+ D+E I A +GVL + +PKL P E K K +++V
Sbjct: 104 RRMGKFMRKFQLPDNA--DLEKISAACNDGVLKVTIPKLPPPEPKKPKTIQVQV 155
>At5g12020 [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 155
Score = 61.2 bits (147), Expect = 9e-10
Identities = 41/117 (35%), Positives = 64/117 (54%), Gaps = 12/117 (10%)
Query: 77 PLDILDHEKEYEVHVSVPGIKEKKDINLEYHKDANEIVISGEIPDVTTEETKDSAKIR-- 134
P D+++H Y V +PGIK +I ++ D N +V+SGE E K++ ++
Sbjct: 46 PADVIEHPNAYAFVVDMPGIKGD-EIKVQVEND-NVLVVSGE----RQRENKENEGVKYV 99
Query: 135 --ERRYGKFSRVVRLPDSPGIDVENIKANYTNGVLVLNVPKLKPTEEDKAKVYKIEV 189
ERR GKF R +LP++ D++ I A +GVL + V KL P E K K +++V
Sbjct: 100 RMERRMGKFMRKFQLPENA--DLDKISAVCHDGVLKVTVQKLPPPEPKKPKTIQVQV 154
>At4g10250 [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 195
Score = 57.0 bits (136), Expect = 2e-08
Identities = 40/112 (35%), Positives = 64/112 (56%), Gaps = 8/112 (7%)
Query: 87 YEVHVSVPGIKEKKDINLEYHKDANEIVISGEIPDVTTEETKDSAKIR-ERRYGKFSRVV 145
+E+ + +PG+K K ++ +E ++ + +SGE EE K R ER YGKF R
Sbjct: 82 HEIMLDIPGLK-KDEVKIEVEENG-VLRVSGERK--REEEKKGDQWHRVERSYGKFWRQF 137
Query: 146 RLPDSPGIDVENIKANYTNGVLVLNVPKLKPTEEDKAKVYKIEV-GDEAAKL 196
+LPD+ +D+E++KA NGVL +N+ KL P + +V I D+ AK+
Sbjct: 138 KLPDN--VDMESVKAKLENGVLTINLTKLSPEKVKGPRVVNIAAEEDQTAKI 187
>At1g59860 [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 155
Score = 56.2 bits (134), Expect = 3e-08
Identities = 41/112 (36%), Positives = 68/112 (60%), Gaps = 9/112 (8%)
Query: 81 LDHEKEYEVHV---SVPGIKEKKDINLEYHKDANEIVISGEIPDVTTEETKDSAKIRERR 137
+D ++ E HV +PG+K K+++ +E D+ + ISGE V EE +D+ ER
Sbjct: 48 VDWKETAEAHVFKADLPGMK-KEEVKVEIEDDS-VLKISGE-RHVEKEEKQDTWHRVERS 104
Query: 138 YGKFSRVVRLPDSPGIDVENIKANYTNGVLVLNVPKLKPTEEDKAKVYKIEV 189
G FSR RLP++ + ++ +KA+ NGVL + VPK++ T + KA+V I++
Sbjct: 105 SGGFSRKFRLPEN--VKMDQVKASMENGVLTVTVPKVE-TNKKKAQVKSIDI 153
>At3g46230 [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 156
Score = 55.1 bits (131), Expect = 6e-08
Identities = 42/112 (37%), Positives = 63/112 (55%), Gaps = 11/112 (9%)
Query: 81 LDHEKEYEVHV---SVPGIKEKKDINLEYHKDANEIVISGEIPDVTTEETKDSAKIRERR 137
+D + E HV VPG+K K+++ +E +D N + ISGE EE D+ ER
Sbjct: 51 VDWRETPEAHVFKADVPGLK-KEEVKVEV-EDGNILQISGERSS-ENEEKSDTWHRVERS 107
Query: 138 YGKFSRVVRLPDSPGIDVENIKANYTNGVLVLNVPKLKPTEEDKAKVYKIEV 189
GKF R RLP++ VE +KA+ NGVL + VPK+ +E K +V +++
Sbjct: 108 SGKFMRRFRLPENA--KVEEVKASMENGVLSVTVPKV---QESKPEVKSVDI 154
>At1g07400 [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 157
Score = 54.7 bits (130), Expect = 8e-08
Identities = 39/112 (34%), Positives = 69/112 (60%), Gaps = 9/112 (8%)
Query: 81 LDHEKEYEVHV---SVPGIKEKKDINLEYHKDANEIVISGEIPDVTTEETKDSAKIRERR 137
+D ++ E HV +PG+K K+++ +E D+ + ISGE V EE +D+ ER
Sbjct: 50 VDWKETAEAHVFKADLPGMK-KEEVKVEIEDDS-VLKISGE-RHVEKEEKQDTWHRVERS 106
Query: 138 YGKFSRVVRLPDSPGIDVENIKANYTNGVLVLNVPKLKPTEEDKAKVYKIEV 189
G+FSR +LP++ + ++ +KA+ NGVL + VPK++ ++ KA+V I++
Sbjct: 107 SGQFSRKFKLPEN--VKMDQVKASMENGVLTVTVPKVEEAKK-KAQVKSIDI 155
>At1g53540 [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 157
Score = 51.6 bits (122), Expect = 7e-07
Identities = 41/112 (36%), Positives = 63/112 (55%), Gaps = 11/112 (9%)
Query: 81 LDHEKEYEVHV---SVPGIKEKKDINLEYHKDANEIVISGEIPDVTTEETKDSAKIRERR 137
+D + E HV +PG++ K+++ +E +D N + ISGE + EE D ER
Sbjct: 52 VDWRETPEAHVFKADLPGLR-KEEVKVEV-EDGNILQISGERSN-ENEEKNDKWHRVERS 108
Query: 138 YGKFSRVVRLPDSPGIDVENIKANYTNGVLVLNVPKLKPTEEDKAKVYKIEV 189
GKF+R RLP++ +E IKA+ NGVL + VPK+ E K +V I++
Sbjct: 109 SGKFTRRFRLPENA--KMEEIKASMENGVLSVTVPKV---PEKKPEVKSIDI 155
>At5g59720 [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 161
Score = 50.4 bits (119), Expect = 2e-06
Identities = 41/112 (36%), Positives = 60/112 (52%), Gaps = 11/112 (9%)
Query: 81 LDHEKEYEVHV---SVPGIKEKKDINLEYHKDANEIVISGEIPDVTTEETKDSAKIRERR 137
+D ++ E HV +PG+K K+++ +E +D N + ISGE EE D ER
Sbjct: 54 VDWKETPEAHVFKADLPGLK-KEEVKVEV-EDKNVLQISGE-RSKENEEKNDKWHRVERA 110
Query: 138 YGKFSRVVRLPDSPGIDVENIKANYTNGVLVLNVPKLKPTEEDKAKVYKIEV 189
GKF R RLP++ +E +KA NGVL + VPK E K +V I++
Sbjct: 111 SGKFMRRFRLPENA--KMEEVKATMENGVLTVVVPK---APEKKPQVKSIDI 157
>At2g29500 [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 153
Score = 50.4 bits (119), Expect = 2e-06
Identities = 39/112 (34%), Positives = 63/112 (55%), Gaps = 11/112 (9%)
Query: 81 LDHEKEYEVHV---SVPGIKEKKDINLEYHKDANEIVISGEIPDVTTEETKDSAKIRERR 137
+D + E HV +PG+K K+++ +E +D+ + ISGE V E+ D+ ER
Sbjct: 48 VDWRETPEAHVFKADLPGLK-KEEVKVEIEEDS-VLKISGE-RHVEKEDKNDTWHRVERS 104
Query: 138 YGKFSRVVRLPDSPGIDVENIKANYTNGVLVLNVPKLKPTEEDKAKVYKIEV 189
G+F+R RLP++ + ++ +KA NGVL + VPK E KA V I++
Sbjct: 105 SGQFTRRFRLPEN--VKMDQVKAAMENGVLTVTVPK---AETKKADVKSIQI 151
>SPCC338.06c [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 139
Score = 50.1 bits (118), Expect = 2e-06
Identities = 33/101 (32%), Positives = 54/101 (52%), Gaps = 5/101 (4%)
Query: 76 PPLDILDHEKEYEVHVSVPGIKEKKDINLEYHKDANEIVISGEIPDVTTEETKDSAKIRE 135
P L++ + E EV V VPGI +K+++ ++ H +++ ISGE E+ + E
Sbjct: 34 PALELRETEDTIEVDVEVPGI-DKQNLKVDLH--GSKLTISGERKKPEEEKAGPLIRWSE 90
Query: 136 RRYGKFSRVVRLPDSPGIDVENIKANYTNGVLVLNVPKLKP 176
R G FSR + LP +D + I A+ NG+L + + K P
Sbjct: 91 RCVGAFSRTITLPQP--VDEKLIHASLNNGILSIVMKKKNP 129
>At1g54050 [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 155
Score = 48.1 bits (113), Expect = 7e-06
Identities = 34/116 (29%), Positives = 57/116 (48%), Gaps = 7/116 (6%)
Query: 77 PLDILDHEKEYEVHVSVPGIKEKKDINLEYHKDANEIVISG---EIPDVTTEETKDSAKI 133
P+DIL+ KEY ++ +PGI K DI + ++ ++ S + D +EE ++
Sbjct: 43 PIDILESPKEYIFYLDIPGI-SKSDIQVTVEEERTLVIKSNGKRKRDDDESEEGSKYIRL 101
Query: 134 RERRYGKFSRVVRLPDSPGIDVENIKANYTNGVLVLNVPKLKPTEEDKAKVYKIEV 189
R + RLP+ D+ ++ A Y GVL + + KL P + K K +I V
Sbjct: 102 ERRLAQNLVKKFRLPED--ADMASVTAKYQEGVLTVVIKKL-PPQPPKPKTVQIAV 154
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.313 0.135 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,698,792
Number of Sequences: 60738
Number of extensions: 480934
Number of successful extensions: 964
Number of sequences better than 1.0e-05: 14
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 945
Number of HSP's gapped (non-prelim): 15
length of query: 196
length of database: 30,389,216
effective HSP length: 101
effective length of query: 95
effective length of database: 24,254,678
effective search space: 2304194410
effective search space used: 2304194410
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)