ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI5141 good J KOG2522 Translation, ribosomal structure and biogenesis Filamentous baseplate protein Ligatin, contains PUA domain
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI5141 1786376 1784667 -570
(570 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR117c [J] KOG2522 Filamentous baseplate protein Ligatin contai... 498 e-140
Hs11386191 [J] KOG2522 Filamentous baseplate protein Ligatin con... 154 3e-37
At1g71350 [J] KOG2522 Filamentous baseplate protein Ligatin cont... 125 1e-28
CE27093 [J] KOG2522 Filamentous baseplate protein Ligatin contai... 83 1e-15
>YDR117c [J] KOG2522 Filamentous baseplate protein Ligatin contains PUA
domain
Length = 565
Score = 498 bits (1281), Expect = e-140
Identities = 264/571 (46%), Positives = 373/571 (65%), Gaps = 9/571 (1%)
Query: 1 MFAKEPHIKPLSNLKNSDRKKLQSTIKRQIN-EEYNLPTAVIKQTSFQVANVAGSIYTDE 59
MF KEPHIK LSNLKNS+RKKL T ++Q N EEY+ T+ IKQT+F G++YTDE
Sbjct: 1 MFKKEPHIKALSNLKNSERKKLLQTFQKQTNNEEYSFRTSTIKQTNFNGQKSVGTVYTDE 60
Query: 60 SNKPIWFKTKFEDQLYPTIFSLWNLQMQLLPIILTHDFVIEKHILGGAQLMLPGTVPPFP 119
+N PI FK K ++QL+PT++S W LLPI+LTH FVIE+H+ GA LM+ G++PPF
Sbjct: 61 NNTPILFKEKHKEQLFPTVYSCWEYPA-LLPIVLTHGFVIEEHLFNGANLMISGSIPPFD 119
Query: 120 SNVKRGSIVGIASTKDPLLVKAIGIAEMDLCGISHVIGTKGIAVRVVHYLDDGLFKAFKL 179
K G++ GIAS + P V AIGI E+DL VIG G+AV+++H+ +DGL K FK+
Sbjct: 120 PRCKIGTLCGIASKQAPETVLAIGIVELDLPSFDKVIGETGVAVKIIHHFNDGLSKVFKM 179
Query: 180 ELEPPAVVYN-SEPIETTAIETQTSKYGEQLDEESNHLERSSSQSPVNEIADELEEMSIE 238
+LEPP V+ S+ ++ + ++S+ + +++E ++ +S V EIA+ L+ ++
Sbjct: 180 KLEPPFVLSTQSKDNNISSKQIESSEQIKAVEKEQEDVKEASVD--VEEIAEVLDHFTVS 237
Query: 239 DVDHMLMRAVKYSIKFDDKITLPIVASTFISNHVMKNLPDVDTNMVNIKKSSWKKATKLL 298
DVD+ + RA+ Y++ D + LPI AS FISNH+M+NLP +D N VN+KK+SWKK+ K L
Sbjct: 238 DVDYFITRALYYTLTQDKGLELPISASNFISNHIMRNLPPIDHNEVNVKKTSWKKSAKFL 297
Query: 299 KYLEDEGFLKLKGKNDSCTVISLNKNHKDLTNMEAYKTLATIAKKSSGDTTNQSNKNKIT 358
K+ E EGFLKLKGK D T++ N + +L N YK + + S ++T K
Sbjct: 298 KHFEKEGFLKLKGKGDDLTIVGKNTDKDELKNFVPYKLGCSKSATESRESTTSKEKTSGM 357
Query: 359 AYSGENLYKPMSACQRFITGITADVPQSYYTQQELKKLLDLYVANNNLIDPENKSLVKYD 418
YS LYKP + + + + +YYT Q+++ + Y++ NL D ++K V D
Sbjct: 358 MYS-LTLYKPFNLAKDLLKEVNL-ASHTYYTSQDIRSAVSQYISVKNLADTKDKGKVIMD 415
Query: 419 DTLYQMSNRAPQNGGIQKI-ARGLIVSPTFLSKNFNTYHQIYKPNGEPLLKVPEKGPIPT 477
D L+ M N+ + +I ARG I+ P L+ NF ++QI+K + L K P KG +P
Sbjct: 416 DLLFDMVNKKKKVLNASRIIARGEILHP-LLTNNFTEFYQIFKSDDTLLFKAPMKGSLPH 474
Query: 478 LEIVTEKKIGRKVITRVTNFEKFKIDSEELAAALRKKCSGSTTIGESKSTKTAEVQVQGP 537
++I+TE KIGRKVITRV+NFE F++D E LAA LRK CSGSTTI ES++ K AEVQVQGP
Sbjct: 475 IKIITEMKIGRKVITRVSNFEVFQVDPESLAADLRKICSGSTTISESQTFKCAEVQVQGP 534
Query: 538 HGSIVISILNDYGIPTKWISFENKVKPKQKK 568
HG +I LN GIP+KWI FENK+K K++K
Sbjct: 535 HGQSIIDHLNKLGIPSKWIDFENKLKKKKRK 565
>Hs11386191 [J] KOG2522 Filamentous baseplate protein Ligatin contains PUA
domain
Length = 584
Score = 154 bits (389), Expect = 3e-37
Identities = 154/618 (24%), Positives = 279/618 (44%), Gaps = 84/618 (13%)
Query: 1 MFAKEPHIKPLSNLKNSDRKKLQSTIKRQINEEYNLPTAVIKQTSFQVANV--------- 51
MFAK +K + +K SDR+KL++ + PT Q S V
Sbjct: 1 MFAKAFRVKSDTAIKGSDRRKLRADVTTAF------PTLGTDQVSELVPGKEELNIVKLY 54
Query: 52 -----AGSIYTDESNKPIWFKTKFEDQLYPTIFSLWNLQMQLLPIILTHDFVIEKHILGG 106
A ++Y N PI F+ E LYPT+++LW+ LLP T V+EK ++GG
Sbjct: 55 AHKGDAVTVYVSGGN-PILFE--LEKNLYPTVYTLWSYP-DLLPTFTTWPLVLEK-LVGG 109
Query: 107 AQLMLPGTVPPFPS--NVKRGSIVGIASTKDPLLVKAIGIAEMDLCGISHVIGTKGIAVR 164
A LMLPG V P V++G + I+ + V AIG+A M + G KG
Sbjct: 110 ADLMLPGLVMPPAGLPQVQKGDLCAISLVGNRAPV-AIGVAAMSTAEML-TSGLKGRGFS 167
Query: 165 VVHYLDDGLFKAFKLELEPPAVVYNSEPIETTAIETQTSKYGEQLD-------------- 210
V+H D L+++ + PP++ P+ + + K Q+D
Sbjct: 168 VLHTYQDHLWRSGN-KSSPPSIA----PLALDSADLSEEKGSVQMDSTLQGDMRHMTLEG 222
Query: 211 -EESNHLERSSSQSPVNEIADELEEMSI-----------EDVDHMLMRAVKYSIKFD-DK 257
EE+ + ++ ++E ++ + E +D +L + +++K K
Sbjct: 223 EEENGEVHQAREDKSLSEAPEDTSTRGLNQDSTDSKTLQEQMDELLQQCFLHALKCRVKK 282
Query: 258 ITLPIVASTFISNHVMKNLPDVDTNMVNIKKSSWKKATKLLKYLEDEGFLKLKGKNDSC- 316
LP++ STF+ +H+ P+ ++IKKSS+KK +K L+ ++ E +++K +
Sbjct: 283 ADLPLLTSTFLGSHMFSCCPE--GRQLDIKKSSYKKLSKFLQQMQQEQIIQVKELSKGVE 340
Query: 317 TVISLNKNHKDLTNMEAYKTLATIAKKSSGDTTNQSNKNKITAYSGENLYKPMSACQRFI 376
++++++ H +T+ I + S T Q + + + KP+ +
Sbjct: 341 SIVAVDWKHPRITSF-------VIPEPSPTSQTIQEGSREQPYHPPD--IKPLYCVPASM 391
Query: 377 TGITADVPQ---SYYTQQELKKLLDLYVANNNLIDPENKSLVKYDDTLYQMSNRAPQNGG 433
T + + S+ E++ ++ Y N+L+D +NK+LV+ D L +
Sbjct: 392 TLLFQESGHKKGSFLEGSEVRTIVINYAKKNDLVDADNKNLVRLDPILCDCILEKNEQHT 451
Query: 434 IQKIARGLIVSPTFLSKNFNTYHQIYKPNGEPLLKVPEKGPIPTLEIVTEKKIGRKVITR 493
+ K+ ++ T + +Q+ P EP++K KG I ++I ++ K +T
Sbjct: 452 VMKLPWDSLL--TRCLEKLQPAYQVTLPGQEPIVK---KGRICPIDITLAQRASNKKVTV 506
Query: 494 VTNFEKFKIDSEELAAALRKKCSGSTTIGESKSTK-TAEVQVQGPH-GSIVISILNDYGI 551
V N E + +D +AA L+++C STT+ + K + +VQ+QG + +L +Y +
Sbjct: 507 VRNLEAYGLDPYSVAAILQQRCQASTTVNPAPGAKDSLQVQIQGNQVHHLGWLLLEEYQL 566
Query: 552 PTKWI-SFENKVKPKQKK 568
P K I E +KP +KK
Sbjct: 567 PRKHIQGLEKALKPGKKK 584
>At1g71350 [J] KOG2522 Filamentous baseplate protein Ligatin contains PUA
domain
Length = 566
Score = 125 bits (315), Expect = 1e-28
Identities = 145/618 (23%), Positives = 259/618 (41%), Gaps = 108/618 (17%)
Query: 1 MFAKEPHIKPLSNLKNSDRKKLQSTIKRQIN-------EEYNLPTAVIKQTSFQVANVAG 53
MF K K L +DRKKL+ T++ ++ + P I + FQ +
Sbjct: 1 MFKKAVEAKSHQRLSGADRKKLRRTVRNRLPLLTDELLDAILPPKVEITLSKFQNRVI-- 58
Query: 54 SIYTDESNKPIWFKTKFED-QLYPTIFSLWNLQMQLLPIILTHDFVIEKHILGGAQLMLP 112
+Y+ E P++F +++PT+F+LW ++LP ML
Sbjct: 59 -VYSIEGGCPMFFDIDGRGTEIFPTVFALWEAP-EMLP-----------------SFMLK 99
Query: 113 GTVPPFPSNVKRGSI--VGIASTKDPLLVKAIGIAEMDLCGISHVIGTKGIAVRVVHYLD 170
G P F + G I V + P+ V ++ + + G +G A+R+ HY
Sbjct: 100 GGYPSFSA----GEIWAVKVPGNLAPIAVGCTTMSSEE----ARKAGLRGKALRITHYYR 151
Query: 171 DGLFKAFK------------LELEPPAVVY--NSEPIETTAIETQTSKYGEQLDEESNHL 216
D L+++ + + +E P+ + + E I T+ T + L+ S
Sbjct: 152 DFLWESAEGHYVPNAGFMEDVVMEDPSYLAPGSGEDILDTSASTGNEEDESALETASTSA 211
Query: 217 ERSSSQSPVNEIADELEEMSIEDV----------------------DHMLMRAVKYSIKF 254
+ + + V+ + E ++DV D L++A+ ++K
Sbjct: 212 ADAKNDAEVHVVGSMNELYLVDDVCATETNTDKQITLSPEEVDTLLDQCLLQALHTTLKE 271
Query: 255 DDKITLPIVASTFISNHVMKNLPDVDTNMVNIKKSSWKKATKLLKYLEDEGFLKLKGKND 314
D LPI ST + + ++IKKSS KK +K L+ G + +K
Sbjct: 272 KD---LPIPGSTLWPSGLT----------LDIKKSSHKKLSKWLQSKASGGMISVKEDKH 318
Query: 315 S--CTVISLNKNHKDLTNMEAYKTLATIAKKSSGDTTNQSNKNKITAYSGENLYKPMSAC 372
+IS+N++H D + + K A +++ +T Q+ K +YK
Sbjct: 319 KKEIVLISVNRSHPDYKSFKPEKKKAEVSESPGERSTAQAQSEK--GLEIIEVYKSSIHN 376
Query: 373 QRFITGITADVPQSYYTQQELKKLLDLYVANNNLIDPENKSLVKYDDTLYQMSNRAPQNG 432
+ D + YT E ++ Y+ NL+ P NKS+V D L A G
Sbjct: 377 SAIFASVGED-KGNLYTASEATDVVFKYIEKENLVKPTNKSMVVLDPILCD----ALFKG 431
Query: 433 GIQKIA------RGLIVSPTFLSKNFNTYHQIYKPNGEPLLKVPEKGPIPTLEIVTEKKI 486
I+K + V TF+ + H + + GEP+++ KG + ++I+TE++
Sbjct: 432 AIKKGSAYPSEIHKKDVGSTFVGR-MQPNHVVMRGGGEPVVR---KGAVKPVQIMTERRQ 487
Query: 487 GRKVITRVTNFEKFKIDSEELAAALRKKCSGSTTIGESKSTKTAEVQVQ-GPHGSIVISI 545
G K +T+VT E F ID + + L+KK + ST++GE K EV +Q G ++ +
Sbjct: 488 GNKKVTKVTGMETFLIDPDSFGSELQKKFACSTSVGELPGKKGYEVLIQGGVIDNLARYM 547
Query: 546 LNDYGIPTKWISFENKVK 563
+ YG+P ++I +K +
Sbjct: 548 VEHYGVPKRYIEVLDKTR 565
>CE27093 [J] KOG2522 Filamentous baseplate protein Ligatin contains PUA
domain
Length = 485
Score = 83.2 bits (204), Expect = 1e-15
Identities = 127/575 (22%), Positives = 236/575 (40%), Gaps = 98/575 (17%)
Query: 1 MFAKEPHIKPLSNLKNSDRKKLQSTIKRQINEEYNL----PTAVIKQTSFQVANVAGSIY 56
MF K +K +N +NSD +KL + +K ++N E N+ A +K T+F+ + ++Y
Sbjct: 1 MFKKPFTVKKNTNQRNSDSRKLFNRLKEEVNGESNIDKKGQVAQVKLTTFEGTQM--NVY 58
Query: 57 TDESNKPIWFKTKFEDQLYPTIFSLWNLQMQLLPIILTHDFVIEKHILGGAQLMLPGTVP 116
T + P+ F+ +YPT++ +WN Q + P+++ H+ V ++ GA LMLPG
Sbjct: 59 TID-KVPMLFEFSENGNIYPTVYYMWNNQ-KTFPVLICHEPVF-SYLENGADLMLPGP-- 113
Query: 117 PFPSNVKRGSIVGIASTKDPLLVKAIGIAEMDLCGISHVIGTKGIAVRVVHYLDDGLFKA 176
S VG + + EM +CG KG V+V+H D L+
Sbjct: 114 ---------SAVGCS---------MMSSEEMVVCGF------KGKGVQVLHVFRDQLWD- 148
Query: 177 FKLELEPPAVVYNSEPIETTAIETQTSKYGEQLDEESNHLERSSSQSPVNEIADELEEMS 236
F + PP+ + E +E++ S+ E+ DE E + QS + + ++ E +
Sbjct: 149 FGPKGVPPSC--SLEDWNKIGVESEESEEDEETDERK---ELDACQSTLPKEEEKQEVIQ 203
Query: 237 IEDVDHMLMRAVKYSIKFD-DKITLPIVASTFISNHVMKNLPDVDTNMVNIKKSSWKKAT 295
E ++++L R +K + LP+ F + V+ +P D +++KK+ +KK
Sbjct: 204 EEPMENLLTRCFLAGLKHRFTRNQLPMDVGQFYTQCVLSCVP--DGRRLDMKKTHFKKFA 261
Query: 296 KLLKYLEDEGFLKLKGKNDSCTVISLNKNHKDLTNMEAYKTLATIAKKSSGDTTNQSNKN 355
L+ + N ++T+ A+K+ D +
Sbjct: 262 TFLQEI----------------------NELEITDERIVDEAP--AEKAKFDAPVIAEYF 297
Query: 356 KITAYSGENLYKPMSACQRFITGITADVPQSYYTQQELKKLLDLYVANNNLIDPENKSLV 415
IT E K C + T +++K+ + YV N L + V
Sbjct: 298 AIT----EPTLKLFPGCSK----------GDLLTVKQIKEFVTKYVNENKLAAGGS---V 340
Query: 416 KYDDTLYQMSNRAPQNGGIQKIARGLIVSPTFLSKNFNTYHQIYKPNGEPLLKVPEKGPI 475
+ D ++ ++ + + + S+ T+H I P+G +++ K
Sbjct: 341 RLDPIIFSVTKIQTDTTPWANLMKEI------HSRMTATWH-IRWPDGREIVR---KVSP 390
Query: 476 PTLEIVTEKKIGRKVITRVTNFEKFKIDSEELAAALRKK--CSGSTTIGESKSTKTAEVQ 533
P +E E + G K +T + F ID + ++ + T+ E + +V
Sbjct: 391 PRVEFKIENRAGNKKVTLLNGLAMFGIDIRTICHQIQVTGVATSVTSQWEVPGVEGPQVL 450
Query: 534 VQGPHGSIVISIL-NDYGIPTKWISFENKVKPKQK 567
VQG + +L YGI K++ K+K
Sbjct: 451 VQGNQIHFIADLLIKSYGIDKKFMKGTELAVKKKK 485
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.314 0.132 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 34,613,888
Number of Sequences: 60738
Number of extensions: 1524231
Number of successful extensions: 3871
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 3853
Number of HSP's gapped (non-prelim): 4
length of query: 570
length of database: 30,389,216
effective HSP length: 112
effective length of query: 458
effective length of database: 23,586,560
effective search space: 10802644480
effective search space used: 10802644480
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)