ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI5281 good I KOG1478 Lipid transport and metabolism 3-keto sterol reductase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI5281 1830489 1831526 346
(346 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR100w [I] KOG1478 3-keto sterol reductase 494 e-140
SPBC1709.07 [I] KOG1478 3-keto sterol reductase 178 9e-45
Hs7705421 [I] KOG1478 3-keto sterol reductase 82 1e-15
Hs22052524 [I] KOG1478 3-keto sterol reductase 65 1e-10
>YLR100w [I] KOG1478 3-keto sterol reductase
Length = 347
Score = 494 bits (1272), Expect = e-140
Identities = 240/344 (69%), Positives = 283/344 (81%), Gaps = 2/344 (0%)
Query: 5 KVAVVTGTNSNLGLNIVYRLIERSEPEDNLTIVVTSRTLPRVRECIDLIKAFVNTINRTG 64
KVA+VTGTNSNLGLNIV+RLIE + LTIVVTSRTLPRV+E I+ IK F N R
Sbjct: 4 KVAIVTGTNSNLGLNIVFRLIETEDTNVRLTIVVTSRTLPRVQEVINQIKDFYNKSGRVE 63
Query: 65 S--VDYDYLLVDFTNMISILDAHYSLSKRYDHINYFFVNAAQGVYSGIDWLGAVKEVFSS 122
+D+DYLLVDFTNM+S+L+A+Y ++K+Y INY FVNAAQG++ GIDW+GAVKEVF++
Sbjct: 64 DLEIDFDYLLVDFTNMVSVLNAYYDINKKYRAINYLFVNAAQGIFDGIDWIGAVKEVFTN 123
Query: 123 PLEAVTNPTYKIQRVGVKSKDGMGLVFQANVFGPYYLIQKLLPLLQAGQGTVVWVSSIMS 182
PLEAVTNPTYKIQ VGVKSKD MGL+FQANVFGPYY I K+LP L G+ +VW+SSIMS
Sbjct: 124 PLEAVTNPTYKIQLVGVKSKDDMGLIFQANVFGPYYFISKILPQLTRGKAYIVWISSIMS 183
Query: 183 APKYLSLQDIQLLESDVSYEGSKRLVDLLHSATYKEMKKLGIRQYLTHPGIFTSLSFFQY 242
PKYLSL DI+LL+++ SYEGSKRLVDLLH ATYK++KKLGI QY+ PGIFTS SF +Y
Sbjct: 184 DPKYLSLNDIELLKTNASYEGSKRLVDLLHLATYKDLKKLGINQYVVQPGIFTSHSFSEY 243
Query: 243 LNVFTYYGMLFLFYLARWIGSPWHNIQGYKAANAPVYVATMANPHFEKEQMKYGSATFRD 302
LN FTY+GML LFYLAR +GSPWHNI GYKAANAPVYV +ANP+FEK+ +KYGSAT RD
Sbjct: 244 LNFFTYFGMLCLFYLARLLGSPWHNIDGYKAANAPVYVTRLANPNFEKQDVKYGSATSRD 303
Query: 303 GLEYIKTDEVDTTGCEDVYKYISNLKLQWDEKLKDQIKPTRIPL 346
G+ YIKT E+D TG DV+ YI KL+WDEKLKDQI TR P+
Sbjct: 304 GMPYIKTQEIDPTGMSDVFAYIQKKKLEWDEKLKDQIVETRTPI 347
>SPBC1709.07 [I] KOG1478 3-keto sterol reductase
Length = 338
Score = 178 bits (452), Expect = 9e-45
Identities = 110/345 (31%), Positives = 184/345 (52%), Gaps = 19/345 (5%)
Query: 1 MSSSKVAVVTGTNSNLGLNIVYRLIERSEPE-----DNLTIVVTSRTLPRVRECIDLIKA 55
MS K A++TG+NS LG I RL++ +P + T+++T R+ + + +K
Sbjct: 1 MSFRKYALITGSNSGLGFGIATRLLQFYQPRLQDEPEVFTVILTCRSREKAEDACRRLKE 60
Query: 56 FVNTINRTGSVDYDYLLVDFTNMISILDAHYSLSKRYDHINYFFVNAAQGVYSGIDWLGA 115
F + +Y+L+D +NM S+ A ++ R+ +++ ++NA GI WL A
Sbjct: 61 FFPD----RKIRLEYVLLDLSNMASVEAAVQDIATRFPKLDFVYLNAGAWDLEGIQWLKA 116
Query: 116 VKEVFSSPLEAVTNPTYKIQRVGVKSKDGMGLVFQANVFGPYYLIQKLLPLLQAGQGT-V 174
+ +P++A+T+PT+ + G S D +G +F++NVFG +YL +L L T V
Sbjct: 117 IFSTLINPIQALTHPTFYKETAGRVSNDSLGYIFESNVFGHFYLKNRLAELKVLRSSTKV 176
Query: 175 VWVSSIMSAPKYLSLQDIQLLESDVSYEGSKRLVDLLHSATYKEMKK-LGIRQYLTHPGI 233
V SS+++ K L +D+Q + Y+ SKRL+D+LH Y E++K L QYL HPG+
Sbjct: 177 VLTSSLVAEKKSLDFEDLQCFHGEQPYQSSKRLLDVLH---YAELEKGLPFEQYLVHPGL 233
Query: 234 FTSLSFFQYLNVFTYYGMLFLFYLARWIGSPWHNIQGYKAANAPVYVATMANPHFEKEQM 293
T+ + +L FY+ R +GSPWH I Y AA A ++ A A E + +
Sbjct: 234 CTTNMYETFLGPILVMCAKLGFYICRLLGSPWHTISPYVAAFAFLWTALHATK--EDQSI 291
Query: 294 KYGSATFRDGLEYIKTDEVD---TTGCEDVYKYISNLKLQWDEKL 335
K+G+A R G E + + V+ + E +Y++ L +W +KL
Sbjct: 292 KWGAAVTRFGHERVLSTPVELILPSEQEKALEYMTKLYQEWKKKL 336
>Hs7705421 [I] KOG1478 3-keto sterol reductase
Length = 341
Score = 82.0 bits (201), Expect = 1e-15
Identities = 82/341 (24%), Positives = 147/341 (43%), Gaps = 16/341 (4%)
Query: 5 KVAVVTGTNSNLGLNIVYRLIERSEPEDNLTIVVTSRTLPRVRECIDLIKAFVNTINRTG 64
KV ++TG +S +GL + RL+ +D L + + R + + + A T T
Sbjct: 3 KVVLITGASSGIGLALCKRLLAE---DDELHLCLACRNMSKAEAVCAALLASHPTAEVT- 58
Query: 65 SVDYDYLLVDFTNMISILDAHYSLSKRYDHINYFFVNAAQGVYSGIDWLGAVKEVFSSPL 124
+ VD +N+ S+ A L +R+ ++ ++NA ++ +FS +
Sbjct: 59 -----IVQVDVSNLQSVFRASKELKQRFQRLDCIYLNAGIMPNPQLNIKALFFGLFSRKV 113
Query: 125 EAVTNPTYKIQRVGVK-SKDGMGLVFQANVFGPYYLIQKLLPLL--QAGQGTVVWVSSIM 181
+ + + G K + DG+ VF+ NVFG + LI++L PLL ++W SS
Sbjct: 114 IHMFSTAEGLLTQGDKITADGLQEVFETNVFGHFILIRELEPLLCHSDNPSQLIWTSSRS 173
Query: 182 SAPKYLSLQDIQLLESDVSYEGSKRLVDLLHSATYKEMKKLGIRQYLTHPGIFTSLSFFQ 241
+ SL+D Q + Y SK DLL A + + G+ + PG + +
Sbjct: 174 ARKSNFSLEDFQHSKGKEPYSSSKYATDLLSVALNRNFNQQGLYSNVACPGTALTNLTYG 233
Query: 242 YLNVFTYYGMLFLFYLARWIGSPWHNIQGYKAANAPVYVATMANPHFEKEQMKYGSATFR 301
L F + ++ L R+ + + + Y A V++ P +KY SAT
Sbjct: 234 ILPPFIWTLLMPAILLLRFFANAF-TLTPYNGTEALVWLFHQ-KPESLNPLIKYLSATTG 291
Query: 302 DGLEYIKTDEVDTTGCEDVYKYISNLKLQWDEKLKDQIKPT 342
G YI T ++D ED + L+ ++ ++ I+ T
Sbjct: 292 FGRNYIMTQKMDLD--EDTAEKFYQKLLELEKHIRVTIQKT 330
>Hs22052524 [I] KOG1478 3-keto sterol reductase
Length = 262
Score = 65.1 bits (157), Expect = 1e-10
Identities = 56/222 (25%), Positives = 99/222 (44%), Gaps = 12/222 (5%)
Query: 5 KVAVVTGTNSNLGLNIVYRLIERSEPEDNLTIVVTSRTLPRVRECIDLIKAFVNTINRTG 64
KV ++TG +S +GL + RL+ +D L + + R + + + A T T
Sbjct: 3 KVVLITGASSGIGLALCKRLLAE---DDELHLCLACRNMSKAEAVCAALLASHPTAEVT- 58
Query: 65 SVDYDYLLVDFTNMISILDAHYSLSKRYDHINYFFVNAAQGVYSGIDWLGAVKEVFSSPL 124
+ VD +N+ S A L +R+ ++ ++NA ++ + +FS +
Sbjct: 59 -----IVQVDVSNLQSFFRASKELKQRFQRLDRIYLNAGIMPNPQLNIKALLFGLFSRKV 113
Query: 125 EAVTNPTYKIQRVGVK-SKDGMGLVFQANVFGPYYLIQKLLPLL--QAGQGTVVWVSSIM 181
+ + + G K + DG+ VF+ +VFG + LI++L PLL ++W SS
Sbjct: 114 IHMFSTAEGLLTQGDKITADGLQEVFETDVFGHFILIRELEPLLCHSDNPSQLIWTSSRN 173
Query: 182 SAPKYLSLQDIQLLESDVSYEGSKRLVDLLHSATYKEMKKLG 223
+ SL+D Q + Y SK DLL A + + G
Sbjct: 174 ARKSNFSLEDFQHSKGKEPYSSSKYATDLLSVALNRNFQPAG 215
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.137 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,525,996
Number of Sequences: 60738
Number of extensions: 874412
Number of successful extensions: 2262
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 2251
Number of HSP's gapped (non-prelim): 4
length of query: 346
length of database: 30,389,216
effective HSP length: 107
effective length of query: 239
effective length of database: 23,890,250
effective search space: 5709769750
effective search space used: 5709769750
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)