ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI5308 good J KOG4685 Translation, ribosomal structure and biogenesis tRNA splicing endonuclease SEN2
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI5308 1838983 1837877 -369
(369 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR105c [J] KOG4685 tRNA splicing endonuclease SEN2 318 1e-86
Hs13376882 [J] KOG4685 tRNA splicing endonuclease SEN2 99 1e-20
At5g60230 [J] KOG4685 tRNA splicing endonuclease SEN2 71 3e-12
At3g45590 [J] KOG4685 tRNA splicing endonuclease SEN2 68 2e-11
>YLR105c [J] KOG4685 tRNA splicing endonuclease SEN2
Length = 377
Score = 318 bits (814), Expect = 1e-86
Identities = 182/378 (48%), Positives = 235/378 (62%), Gaps = 44/378 (11%)
Query: 29 NQLQYKHALPIE-AQPVPSVWKHPFQAFSWILDWAMRW--SETRIID-VKINQLGQ---- 80
NQ +YK+ LPI +P + H SW+ WA R+ S + D V ++ +G
Sbjct: 7 NQKRYKYPLPIHPVDDLPELILH--NPLSWLY-WAYRYYKSTNALNDKVHVDFIGDTTLH 63
Query: 81 ILVCDQWQMKYLWEHGFFGTGQLSRSEPNWQARTMSRLQLNDADRIN------------- 127
I V D QM YLW +GFFGTGQ SRSEP W+ART +RL LND N
Sbjct: 64 ITVQDDKQMLYLWNNGFFGTGQFSRSEPTWKARTEARLGLNDTPLHNRGGTKSNTETEMT 123
Query: 128 LEHVTDVRRRQRLEFKKLRAEFEAKKLEMRQKGIVDEQFIEQERLFLKNQRDKELAFEKD 187
LE VT RR QRLEFKK RA+ E + LE+R+KG I++E + L+ QR+ F+
Sbjct: 124 LEKVTQQRRLQRLEFKKERAKLERELLELRKKG----GHIDEENILLEKQRESLRKFKLK 179
Query: 188 SMQ---------------IRDEDLRLI-EGKSVIQLESLQLMPVEAIFLSYAVPVLKITL 231
+ +RDED L+ E ++ LESL+LMPVEA+FL++A+PVL I+
Sbjct: 180 QTEDVGIVAQQQDISESNLRDEDNNLLDENGDLLPLESLELMPVEAMFLTFALPVLDISP 239
Query: 232 QQLMVSLVLEPKSFQDIKKLIHQYVAYHHYRSKGWCVRSGIKFGCEYLLYRRGPPFQHAE 291
L L ++DI + YV YHHYRS GWCVRSGIKFGC+YLLY+RGPPFQHAE
Sbjct: 240 ACLAGKLFQFDAKYKDIHSFVRSYVIYHHYRSHGWCVRSGIKFGCDYLLYKRGPPFQHAE 299
Query: 292 YGIMVLDANQSNDYTWYSSAARVVGGAKKQFVLCYVEQLIPEDDLMELWKQSKYDELFRN 351
+ +M LD + S DYTWYSS ARVVGGAKK FVLCYVE+LI E + + LWK + + +LF +
Sbjct: 300 FCVMGLDHDVSKDYTWYSSIARVVGGAKKTFVLCYVERLISEQEAIALWKSNNFTKLFNS 359
Query: 352 FKIHELLYKRWVAGKNRD 369
F++ E+LYKRWV G+NRD
Sbjct: 360 FQVGEVLYKRWVPGRNRD 377
>Hs13376882 [J] KOG4685 tRNA splicing endonuclease SEN2
Length = 465
Score = 98.6 bits (244), Expect = 1e-20
Identities = 58/170 (34%), Positives = 93/170 (54%), Gaps = 14/170 (8%)
Query: 208 ESLQLMPVEAIFLSYAVPVLKITLQQLMVSLVLEPKSFQDIKKLIHQ-YVAYHHYRSKGW 266
E LQL EA FL YA+ L I ++ +++V K+F ++ Y+AYH++RSKGW
Sbjct: 295 EYLQLSLEEAFFLVYALGCLSIYYEKEPLTIVKLWKAFTVVQPTFRTTYMAYHYFRSKGW 354
Query: 267 CVRSGIKFGCEYLLYRRGPPFQHAEYGIMV------LDANQSNDYTWYSSAA--RVVGGA 318
+ G+K+G + LLYR+GPPF HA Y +++ + + +W S AA RV
Sbjct: 355 VPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVNV 414
Query: 319 KKQFVLCYVEQLIPEDDLMELWKQSKYDELFRNFKIHELLYKRWVAGKNR 368
K+ +LCY LI + + K+ + E + K+ E++ RWV+ + R
Sbjct: 415 SKELMLCY---LIKPSTMTD--KEMESPECMKRIKVQEVILSRWVSSRER 459
>At5g60230 [J] KOG4685 tRNA splicing endonuclease SEN2
Length = 250
Score = 70.9 bits (172), Expect = 3e-12
Identities = 53/196 (27%), Positives = 86/196 (43%), Gaps = 18/196 (9%)
Query: 182 LAFEKDSMQIRDEDLRLIEGKSVIQLESL----QLMPVEAIFLSYAVPVLKITLQQLMVS 237
LA E + ++ D R G+ V+ E + QL EA FL Y + +KI+LQ +
Sbjct: 46 LAVEPEQAELLD---RCCFGRLVLSAEKIKKWIQLSFEEAFFLHYNLKCIKISLQGRCLE 102
Query: 238 LVLEPKSFQDIKKLIHQ--YVAYHHYRSKGWCVRSGIKFGCEYLLYRRGPPFQHAEYGIM 295
++ + K+ + AY H RSK W +RSG+++G +++ YR P H+EY ++
Sbjct: 103 NEVDTWLYMKSKRPNFPMFFKAYSHLRSKNWVLRSGLQYGVDFVAYRHHPSLVHSEYSVL 162
Query: 296 VLDANQSNDYTW--YSSAARVVGGAKKQFVLCYVEQLIPEDDLMELWKQSKYDELFRNFK 353
V + W A R+ G K + YV +D+ L NF
Sbjct: 163 VQSGDSDRLRVWSDIHCAVRLSGSVAKTLLTLYVNGNFKGEDVNLL-------VCLENFT 215
Query: 354 IHELLYKRWVAGKNRD 369
+ E RW +R+
Sbjct: 216 VEEQTISRWSPELSRE 231
>At3g45590 [J] KOG4685 tRNA splicing endonuclease SEN2
Length = 237
Score = 67.8 bits (164), Expect = 2e-11
Identities = 49/196 (25%), Positives = 90/196 (45%), Gaps = 24/196 (12%)
Query: 189 MQIRDEDLRLIE----GKSVIQLES----LQLMPVEAIFLSYAVPVLKITLQ----QLMV 236
+ + E+ L++ G+ V+ E +QL EA FL Y + +KI L + V
Sbjct: 46 LSVESEEAELLDRCCFGRLVVGAEKDKRWIQLSFEEAFFLFYKLKCIKICLHGRSLENEV 105
Query: 237 SLVLEPKSFQDIKKLIHQYVAYHHYRSKGWCVRSGIKFGCEYLLYRRGPPFQHAEYGIMV 296
L SF+ ++ Y AY H RSK W VRSG+++G ++++YR P H+EY ++V
Sbjct: 106 DLWRSMSSFKQDFAIL--YKAYSHLRSKNWIVRSGLQYGVDFVVYRHHPSLVHSEYAVLV 163
Query: 297 LDANQSNDYTWYSS---AARVVGGAKKQFVLCYVEQLIPEDDLMELWKQSKYDELFRNFK 353
++ +S + R+ G K ++ YV + + + + ++
Sbjct: 164 QSIGGNDRLKVWSDIHCSVRLTGSVAKSLLVLYVNRKVNTEKM-------NLPLCLEDYT 216
Query: 354 IHELLYKRWVAGKNRD 369
+ E +RW +R+
Sbjct: 217 VEEQTIRRWSPELSRE 232
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.324 0.138 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,644,385
Number of Sequences: 60738
Number of extensions: 970297
Number of successful extensions: 2645
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2636
Number of HSP's gapped (non-prelim): 5
length of query: 369
length of database: 30,389,216
effective HSP length: 108
effective length of query: 261
effective length of database: 23,829,512
effective search space: 6219502632
effective search space used: 6219502632
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)