ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI5401 good H KOG1321 Coenzyme transport and metabolism Protoheme ferro-lyase (ferrochelatase)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI5401 1878839 1877667 -391
(391 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR176w [H] KOG1321 Protoheme ferro-lyase (ferrochelatase) 574 e-164
Hs4557593 [H] KOG1321 Protoheme ferro-lyase (ferrochelatase) 345 7e-95
SPCC320.09 [H] KOG1321 Protoheme ferro-lyase (ferrochelatase) 331 1e-90
7302105 [H] KOG1321 Protoheme ferro-lyase (ferrochelatase) 294 1e-79
At2g30390 [H] KOG1321 Protoheme ferro-lyase (ferrochelatase) 182 1e-45
At5g26030 [H] KOG1321 Protoheme ferro-lyase (ferrochelatase) 172 1e-42
>YOR176w [H] KOG1321 Protoheme ferro-lyase (ferrochelatase)
Length = 393
Score = 574 bits (1480), Expect = e-164
Identities = 278/390 (71%), Positives = 324/390 (82%), Gaps = 5/390 (1%)
Query: 2 MISSALRNRNSAFCSPVL---RNYVHRFHSQ--AKKGPTGIVFMNMGGPSKVSETYDFLY 56
M+S +R + S L R++ F+ Q K+ PTGIV MNMGGPSKV ETYDFLY
Sbjct: 1 MLSRTIRTQGSFLRRSQLTITRSFSVTFNMQNAQKRSPTGIVLMNMGGPSKVEETYDFLY 60
Query: 57 QLFSDYDLIPISAKYQPIIAKYIARFRTPKIEKQYEEIGGGSPIKKWSEYQCARVCELLD 116
QLF+D DLIPISAKYQ IAKYIA+FRTPKIEKQY EIGGGSPI+KWSEYQ VC++LD
Sbjct: 61 QLFADNDLIPISAKYQKTIAKYIAKFRTPKIEKQYREIGGGSPIRKWSEYQATEVCKILD 120
Query: 117 EIHPQTAPHKPYVAFRYARPLTHETYEQMLKDGVSRAVAFSQYPQFSYSTTGSSINELWR 176
+ P+TAPHKPYVAFRYA+PLT ETY+QMLKDGV +AVAFSQYP FSYSTTGSSINELWR
Sbjct: 121 KTCPETAPHKPYVAFRYAKPLTAETYKQMLKDGVKKAVAFSQYPHFSYSTTGSSINELWR 180
Query: 177 QIKNLDPERQISWSVIDRWPTQPGLIKAFAENITRKLEEFPAEIRDNAVILFSAHSLPMD 236
QIK LD ER ISWSVIDRWPT GLIKAF+ENIT+KL+EFP +RD V+LFSAHSLPMD
Sbjct: 181 QIKALDSERSISWSVIDRWPTNEGLIKAFSENITKKLQEFPQPVRDKVVLLFSAHSLPMD 240
Query: 237 VVNTGDAYPAEVAATVYHVMKELNFRNPYRLVWQSQVGPKPWLGAQTSDITEFLAPTQPG 296
VVNTGDAYPAEVAATVY++M++L F+NPYRLVWQSQVGPKPWLGAQT++I EFL P G
Sbjct: 241 VVNTGDAYPAEVAATVYNIMQKLKFKNPYRLVWQSQVGPKPWLGAQTAEIAEFLGPKVDG 300
Query: 297 LVLVPIAFTSDHIETLHEVDLGIIAESKYKDKIKRCDSLNGSETYIKGMVDLVKNHLDNG 356
L+ +PIAFTSDHIETLHE+DLG+I ES+YKDK KRC+SLNG++T+I+GM DLVK+HL +
Sbjct: 301 LMFIPIAFTSDHIETLHEIDLGVIGESEYKDKFKRCESLNGNQTFIEGMADLVKSHLQSN 360
Query: 357 PRFSNQLSLDFTLGKSSDPIISAAEVFAEN 386
+SNQL LDF LGKS+DP+ + VF +
Sbjct: 361 QLYSNQLPLDFALGKSNDPVKDLSLVFGNH 390
>Hs4557593 [H] KOG1321 Protoheme ferro-lyase (ferrochelatase)
Length = 423
Score = 345 bits (885), Expect = 7e-95
Identities = 177/347 (51%), Positives = 235/347 (67%), Gaps = 10/347 (2%)
Query: 29 QAKKGPTGIVFMNMGGPSKVSETYDFLYQLFSDYDL--IPISAKYQPIIAKYIARFRTPK 86
Q +K TGI+ +NMGGP + + +DFL +LF D DL +PI K P IAK RTPK
Sbjct: 63 QKRKPKTGILMLNMGGPETLGDVHDFLLRLFLDQDLMTLPIQNKLAPFIAKR----RTPK 118
Query: 87 IEKQYEEIGGGSPIKKWSEYQCARVCELLDEIHPQTAPHKPYVAFRYARPLTHETYEQML 146
I++QY IGGGSPIK W+ Q + +LLDE+ P TAPHK Y+ FRY PLT E E+M
Sbjct: 119 IQEQYRRIGGGSPIKIWTSKQGEGMVKLLDELSPNTAPHKYYIGFRYVHPLTEEAIEEME 178
Query: 147 KDGVSRAVAFSQYPQFSYSTTGSSINELWRQIKNLDPERQISWSVIDRWPTQPGLIKAFA 206
+DG+ RA+AF+QYPQ+S STTGSS+N ++R + + + WS IDRWPT LI+ FA
Sbjct: 179 RDGLERAIAFTQYPQYSCSTTGSSLNAIYRYYNQVGRKPTMKWSTIDRWPTHHLLIQCFA 238
Query: 207 ENITRKLEEFPAEIRDNAVILFSAHSLPMDVVNTGDAYPAEVAATVYHVMKELNFRNPYR 266
++I ++L+ FP E R VILFSAHSLPM VVN GD YP EV+ATV VM+ L + NPYR
Sbjct: 239 DHILKELDHFPLEKRSEVVILFSAHSLPMSVVNRGDPYPQEVSATVQKVMERLEYCNPYR 298
Query: 267 LVWQSQVGPKPWLGAQTSDITEFLAPT-QPGLVLVPIAFTSDHIETLHEVDL---GIIAE 322
LVWQS+VGP PWLG QT + + L + ++LVPIAFTSDHIETL+E+D+ ++A+
Sbjct: 299 LVWQSKVGPMPWLGPQTDESIKGLCERGRKNILLVPIAFTSDHIETLYELDIEYSQVLAK 358
Query: 323 SKYKDKIKRCDSLNGSETYIKGMVDLVKNHLDNGPRFSNQLSLDFTL 369
+ I+R +SLNG+ + K + DLV +H+ + S QL+L L
Sbjct: 359 ECGVENIRRAESLNGNPLFSKALADLVHSHIQSNELCSKQLTLSCPL 405
>SPCC320.09 [H] KOG1321 Protoheme ferro-lyase (ferrochelatase)
Length = 384
Score = 331 bits (849), Expect = 1e-90
Identities = 170/340 (50%), Positives = 231/340 (67%), Gaps = 5/340 (1%)
Query: 28 SQAKKGPTGIVFMNMGGPSKVSETYDFLYQLFSDYDLIPISAKYQPIIAKYIARFRTPKI 87
S + KGPT +V MNMGGPS + E FL +LF+D D+IP+ +Q + K+IA+ RTPK+
Sbjct: 26 SVSGKGPTAVVMMNMGGPSNLDEVGPFLERLFTDGDIIPLGY-FQNSLGKFIAKRRTPKV 84
Query: 88 EKQYEEIGGGSPIKKWSEYQCARVCELLDEIHPQTAPHKPYVAFRYARPLTHETYEQMLK 147
+ Y +IGGGSPI W+ Q + +C++LD+ P++APH P+VAFRYA PLT + +++ K
Sbjct: 85 QNHYSDIGGGSPILHWTRIQGSEMCKILDKKCPESAPHLPFVAFRYAPPLTEDMLDELKK 144
Query: 148 DGVSRAVAFSQYPQFSYSTTGSSINELWRQIKNLDPERQISWSVIDRWPTQPGLIKAFAE 207
VSRAVAFSQYPQ+S +T+G+S+NEL R++ E+ WS++DRWP Q GLI AFAE
Sbjct: 145 ANVSRAVAFSQYPQWSCATSGASLNELRRKLIEKGMEKDFEWSIVDRWPLQQGLINAFAE 204
Query: 208 NITRKLEEFPAEIRDNAVILFSAHSLPMDVVNTGDAYPAEVAATVYHVMKELNFRNPYRL 267
NI L+ +P ++RD+ VI+FSAHSLPM V GD Y E+AAT VMK LN++N +
Sbjct: 205 NIEETLKTYPEDVRDDVVIVFSAHSLPMSQVAKGDPYVYEIAATSQAVMKRLNYKNKFVN 264
Query: 268 VWQSQVGPKPWLGAQTSDITEFLAPT-QPGLVLVPIAFTSDHIETLHEVDLGIIAESKYK 326
WQS+VGP PW+ T + E L Q ++LVPIAFTSDHIETL E++ I ++K K
Sbjct: 265 AWQSKVGPLPWMSPATDFVIEQLGNRGQKNMILVPIAFTSDHIETLKELE-DYIEDAKQK 323
Query: 327 --DKIKRCDSLNGSETYIKGMVDLVKNHLDNGPRFSNQLS 364
+KR S+NGS T I+GM DLV HL +S Q +
Sbjct: 324 GITGVKRVSSINGSMTAIQGMADLVAEHLKAKVPYSRQFT 363
>7302105 [H] KOG1321 Protoheme ferro-lyase (ferrochelatase)
Length = 384
Score = 294 bits (753), Expect = 1e-79
Identities = 148/327 (45%), Positives = 213/327 (64%), Gaps = 6/327 (1%)
Query: 31 KKGPTGIVFMNMGGPSKVSETYDFLYQLFSDYDLIPISAKYQPIIAKYIARFRTPKIEKQ 90
+K T I+ +NMGGP+ + +D+L ++ +D D+I + Q + +IA+ RTP+++K+
Sbjct: 25 QKPKTAILMLNMGGPTHTDQVHDYLLRIMTDRDMIQLPV--QSRLGPWIAQRRTPEVQKK 82
Query: 91 YEEIGGGSPIKKWSEYQCARVCELLDEIHPQTAPHKPYVAFRYARPLTHETYEQMLKDGV 150
Y+EIGGGSPI KW+E Q +CE LD I P+TAPHK YV FRY PLT T ++ KD
Sbjct: 83 YKEIGGGSPILKWTELQGQLMCEQLDRISPETAPHKHYVGFRYVNPLTENTLAEIEKDKP 142
Query: 151 SRAVAFSQYPQFSYSTTGSSINELWRQIKNLDPERQISWSVIDRWPTQPGLIKAFAENIT 210
R V FSQYPQ+S +T+GSS N ++ ++ + I WS+IDRW T P LIK FA+ I
Sbjct: 143 ERVVLFSQYPQYSCATSGSSFNSIFTHYRSNNLPSDIKWSIIDRWGTHPLLIKTFAQRIR 202
Query: 211 RKLEEFPAEIRDNAVILFSAHSLPMDVVNTGDAYPAEVAATVYHVMKELNFRNPYRLVWQ 270
+L +F R++ VILF+AHSLP+ VN GDAYP+E+ A+V+ VM+EL NPY L WQ
Sbjct: 203 DELAKFVETKRNDVVILFTAHSLPLKAVNRGDAYPSEIGASVHMVMQELGQTNPYSLAWQ 262
Query: 271 SQVGPKPWLGAQTSD-ITEFLAPTQPGLVLVPIAFTSDHIETLHEVDLGI---IAESKYK 326
S+VGP PWL T D I ++ +LVPIAF ++HIETLHE+D+ +A+
Sbjct: 263 SKVGPLPWLAPATDDAIKGYVKQGLKNFILVPIAFVNEHIETLHELDIEYCDELAKEVGV 322
Query: 327 DKIKRCDSLNGSETYIKGMVDLVKNHL 353
++I+R + N +I + ++V +HL
Sbjct: 323 EEIRRAATPNDHPLFIDALTNVVADHL 349
>At2g30390 [H] KOG1321 Protoheme ferro-lyase (ferrochelatase)
Length = 512
Score = 182 bits (461), Expect = 1e-45
Identities = 121/329 (36%), Positives = 177/329 (53%), Gaps = 17/329 (5%)
Query: 36 GIVFMNMGGPSKVSETYDFLYQLFSDYDLI---PISAKYQPIIAKYIARFRTPKIEKQYE 92
G++ +N+GGP + + FL+ LF+D D+I P+ Q +A++I+ R PK ++ Y
Sbjct: 102 GVLLLNLGGPETLDDVQPFLFNLFADPDIIRLPPVFQFLQKPLAQFISVARAPKSKEGYA 161
Query: 93 EIGGGSPIKKWSEYQCARVCELLDEIHPQTAPHKPYVAFRYARPLTHETYEQMLKDGVSR 152
IGGGSP++ ++ Q + + L E + P K YV RY P T E EQ+ +DG+++
Sbjct: 162 SIGGGSPLRHITDAQAEELRKCLWE---KNVPAKVYVGMRYWHPFTEEAIEQIKRDGITK 218
Query: 153 AVAFSQYPQFSYSTTGSSINELWRQIKNLDPERQISWSVIDRWPTQPGLIKAFAENITRK 212
V YPQFS ST+GSS+ L R + + + +VI W + G IKA A I +
Sbjct: 219 LVVLPLYPQFSISTSGSSLRLLERIFREDEYLVNMQHTVIPSWYQREGYIKAMANLIQSE 278
Query: 213 LEEFPAEIRDNAVILFSAHSLPMDVV-NTGDAYPAEVAATVYHVMKELNFR---NPYRLV 268
L +F + + VI FSAH +P+ V GD Y AE+ V +M+EL+ R N Y L
Sbjct: 279 LGKFGSP--NQVVIFFSAHGVPLAYVEEAGDPYKAEMEECVDLIMEELDKRKITNAYTLA 336
Query: 269 WQSQVGPKPWLGAQTSD-ITEFLAPTQPGLVLVPIAFTSDHIETLHEVDLGIIAESKYKD 327
+QS+VGP WL T + ITE L+ VPI+F S+HIETL E+D+ E K
Sbjct: 337 YQSRVGPVEWLKPYTEEAITELGKKGVENLLAVPISFVSEHIETLEEIDVE-YKELALKS 395
Query: 328 KIK---RCDSLNGSETYIKGMVDLVKNHL 353
IK R +L +I + D V L
Sbjct: 396 GIKNWGRVPALGTEPMFISDLADAVVESL 424
>At5g26030 [H] KOG1321 Protoheme ferro-lyase (ferrochelatase)
Length = 466
Score = 172 bits (435), Expect = 1e-42
Identities = 116/361 (32%), Positives = 188/361 (51%), Gaps = 20/361 (5%)
Query: 36 GIVFMNMGGPSKVSETYDFLYQLFSDYDLIPISAKYQPI---IAKYIARFRTPKIEKQYE 92
G++ +N+GGP +++ FLY LF+D D+I + +Q + IAK+I+ R PK ++ Y
Sbjct: 91 GVLLLNLGGPETLNDVQPFLYNLFADPDIIRLPRPFQFLQGTIAKFISVVRAPKSKEGYA 150
Query: 93 EIGGGSPIKKWSEYQCARVCELLDEIHPQTAPHKPYVAFRYARPLTHETYEQMLKDGVSR 152
IGGGSP++K ++ Q + L + YV RY P T E +Q+ KD ++R
Sbjct: 151 AIGGGSPLRKITDEQADAIKMSLQA---KNIAANVYVGMRYWYPFTEEAVQQIKKDKITR 207
Query: 153 AVAFSQYPQFSYSTTGSSINELWRQIKNLDPERQISWSVIDRWPTQPGLIKAFAENITRK 212
V YPQ+S STTGSSI L + + ++I W + G + + A+ I ++
Sbjct: 208 LVVLPLYPQYSISTTGSSIRVLQDLFRKDPYLAGVPVAIIKSWYQRRGYVNSMADLIEKE 267
Query: 213 LEEF--PAEIRDNAVILFSAHSLPMDVV-NTGDAYPAEVAATVYHVMKELNFR---NPYR 266
L+ F P E+ +I FSAH +P+ V N GD Y ++ + +M+EL R N ++
Sbjct: 268 LQTFSDPKEV----MIFFSAHGVPVSYVENAGDPYQKQMEECIDLIMEELKARGVLNDHK 323
Query: 267 LVWQSQVGPKPWLGAQTSDITEFLAPT-QPGLVLVPIAFTSDHIETLHEVDLGI--IAES 323
L +QS+VGP WL T ++ L + L+ VP++F S+HIETL E+D+ +A
Sbjct: 324 LAYQSRVGPVQWLKPYTDEVLVDLGKSGVKSLLAVPVSFVSEHIETLEEIDMEYRELALE 383
Query: 324 KYKDKIKRCDSLNGSETYIKGMVDLVKNHLDNGPRFSN-QLSLDFTLGKSSDPIISAAEV 382
+ R +L + ++I + D V L + SN +D +SSD ++
Sbjct: 384 SGVENWGRVPALGLTPSFITDLADAVIESLPSAEAMSNPNAVVDSEDSESSDAFSYIVKM 443
Query: 383 F 383
F
Sbjct: 444 F 444
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,902,131
Number of Sequences: 60738
Number of extensions: 1104554
Number of successful extensions: 2469
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2445
Number of HSP's gapped (non-prelim): 6
length of query: 391
length of database: 30,389,216
effective HSP length: 108
effective length of query: 283
effective length of database: 23,829,512
effective search space: 6743751896
effective search space used: 6743751896
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)