ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI5477 good IT KOG1116 Lipid transport and metabolism Sphingosine kinase, involved in sphingolipid metabolism
r_klactVI5477 good IT KOG1116 Signal transduction mechanisms Sphingosine kinase, involved in sphingolipid metabolism
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI5477 1904429 1902729 -567
(567 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR260w [IT] KOG1116 Sphingosine kinase involved in sphingolipid... 568 e-161
YOR171c [IT] KOG1116 Sphingosine kinase involved in sphingolipid... 552 e-157
SPAC4A8.07c [IT] KOG1116 Sphingosine kinase involved in sphingol... 207 3e-53
At4g21540 [IT] KOG1116 Sphingosine kinase involved in sphingolip... 117 6e-26
HsM11464967 [IT] KOG1116 Sphingosine kinase involved in sphingol... 103 5e-22
Hs21361088 [IT] KOG1116 Sphingosine kinase involved in sphingoli... 103 5e-22
HsM9910562 [IT] KOG1116 Sphingosine kinase involved in sphingoli... 98 3e-20
Hs21361699 [IT] KOG1116 Sphingosine kinase involved in sphingoli... 98 3e-20
7292298_1 [IT] KOG1116 Sphingosine kinase involved in sphingolip... 95 3e-19
CE03053 [IT] KOG1116 Sphingosine kinase involved in sphingolipid... 94 7e-19
7292647 [IT] KOG1116 Sphingosine kinase involved in sphingolipid... 93 9e-19
At5g23450 [IT] KOG1116 Sphingosine kinase involved in sphingolip... 87 9e-17
Hs20336726 [IT] KOG1115 Ceramide kinase 82 2e-15
7296762 [IT] KOG1115 Ceramide kinase 80 6e-15
CE18241 [IT] KOG1115 Ceramide kinase 69 2e-11
Hs8922701 [IT] KOG4435 Predicted lipid kinase 68 3e-11
At2g46090 [IT] KOG1116 Sphingosine kinase involved in sphingolip... 58 4e-08
At5g51290 [IT] KOG1115 Ceramide kinase 51 4e-06
>YLR260w [IT] KOG1116 Sphingosine kinase involved in sphingolipid metabolism
Length = 687
Score = 568 bits (1463), Expect = e-161
Identities = 276/477 (57%), Positives = 358/477 (74%), Gaps = 36/477 (7%)
Query: 125 TALIEVTFAKPRRNHLVPKTIKLSVD----TSVFPNMGTDVAEEILNRSYKNTKRNKSIL 180
T L+EV FA+PRR+ +VPK + L +D +S D+ EEIL RSYKNT+RNKSI
Sbjct: 213 TKLVEVIFARPRRHDVVPKRVSLYIDYKPHSSSHLKEEDDLVEEILKRSYKNTRRNKSIF 272
Query: 181 VIINPHGGKGKANKLFLSKAKPILLASGCKIVVKETTYHLHAVDIAKNLDIDEYDVVACA 240
VIINP GGKGKA KLF++KAKP+LLAS C I V T Y HA++IA+ +DID+YD +ACA
Sbjct: 273 VIINPFGGKGKAKKLFMTKAKPLLLASRCSIEVVYTKYPGHAIEIAREMDIDKYDTIACA 332
Query: 241 SGDGIPHEIMNGLFLRSDRAKAFQKLAITQLPCGSGNAMSISCHGTSNPSYAALALLKSV 300
SGDGIPHE++NGL+ R D KAF +AIT++PCGSGNAMS+SCH T+NPSY+ L L+KS+
Sbjct: 333 SGDGIPHEVINGLYQRPDHVKAFNNIAITEIPCGSGNAMSVSCHWTNNPSYSTLCLIKSI 392
Query: 301 ESRIDVMCCTQPSY-KNQPRLSFLSQTYGIIAESDIDTEFIRFIGPARFELGVTMNVLQR 359
E+RID+MCC+QPSY + P+LSFLSQTYG+IAE+DI+TEFIR++GPARFELGV N++Q+
Sbjct: 393 ETRIDLMCCSQPSYAREHPKLSFLSQTYGLIAETDINTEFIRWMGPARFELGVAFNIIQK 452
Query: 360 KKYPCDIYFKYAAKTKNELKDHYLQHKDRLN------NLRNDLESSSTFTLD-------- 405
KKYPC+IY KYAAK+KNELK+HYL+HK++ + + D E + +
Sbjct: 453 KKYPCEIYVKYAAKSKNELKNHYLEHKNKGSLEFQHITMNKDNEDCDNYNYENEYETENE 512
Query: 406 ---------------IPRSGKESNLDDITITDDSFKLRWPFEDDVPSDWERIDPELTRNT 450
I R +S+ D I ++ FK+++P ++ +PSDWER+DP ++ N
Sbjct: 513 DEDEDADADDEDSHLISRDLADSSADQ--IKEEDFKIKYPLDEGIPSDWERLDPNISNNL 570
Query: 451 GIFYTGKMPYIAPDTKFFPAALPDDGTMDFVLTDGRTPLTRIAPILMSLNRGSHVLQPEV 510
GIFYTGKMPY+A DTKFFPAALP DGTMD V+TD RT LTR+APIL+ L++GSHVLQPEV
Sbjct: 571 GIFYTGKMPYVAADTKFFPAALPSDGTMDMVITDARTSLTRMAPILLGLDKGSHVLQPEV 630
Query: 511 EHSKILAFRLVPYAKNSVISVDGENFPYETLQVEVLPKLVKTLLKNGSYVETDFESM 567
HSKILA++++P N + SVDGE FP E LQVE++P+L KTLL+NG YV+TDF+SM
Sbjct: 631 LHSKILAYKIIPKLGNGLFSVDGEKFPLEPLQVEIMPRLCKTLLRNGRYVDTDFDSM 687
>YOR171c [IT] KOG1116 Sphingosine kinase involved in sphingolipid metabolism
Length = 624
Score = 552 bits (1422), Expect = e-157
Identities = 273/461 (59%), Positives = 355/461 (76%), Gaps = 17/461 (3%)
Query: 123 DHTALIEVTFAKPRRNHLVPKTIKLSVD--------TSVFPNMGTDVAEEILNRSYKNTK 174
D+ LIE+TFA P+ + ++P+ + L +D + N+ + EEIL +SY+N+K
Sbjct: 165 DNQQLIELTFAVPKGHDVIPQKLTLLIDHVSRKSRANTGEENISSGTVEEILEKSYENSK 224
Query: 175 RNKSILVIINPHGGKGKANKLFLSKAKPILLASGCKIVVKETTYHLHAVDIAKNLDIDEY 234
RN+SILVIINPHGGKG A LFL+KA+PIL+ SGCKI + T Y HA+DIAK+LDI +Y
Sbjct: 225 RNRSILVIINPHGGKGTAKNLFLTKARPILVESGCKIEIAYTKYARHAIDIAKDLDISKY 284
Query: 235 DVVACASGDGIPHEIMNGLFLRSDRAKAFQKLAITQLPCGSGNAMSISCHGTSNPSYAAL 294
D +ACASGDGIP+E++NGL+ R DR AF KLA+TQLPCGSGNAMSISCH T+NPSYAAL
Sbjct: 285 DTIACASGDGIPYEVINGLYRRPDRVDAFNKLAVTQLPCGSGNAMSISCHWTNNPSYAAL 344
Query: 295 ALLKSVESRIDVMCCTQPSYKNQ-PRLSFLSQTYGIIAESDIDTEFIRFIGPARFELGVT 353
L+KS+E+RID+MCC+QPSY N+ PRLSFLSQTYG+IAESDI+TEFIR++GP RF LGV
Sbjct: 345 CLVKSIETRIDLMCCSQPSYMNEWPRLSFLSQTYGVIAESDINTEFIRWMGPVRFNLGVA 404
Query: 354 MNVLQRKKYPCDIYFKYAAKTKNELKDHYLQHKDR----LNNLRNDLESSSTFTL---DI 406
N++Q KKYPC+++ KYAAK+K ELK H+L++KD+ L N +SS L +I
Sbjct: 405 FNIIQGKKYPCEVFVKYAAKSKKELKVHFLENKDKNKGCLTFEPNPSPNSSPDLLSKNNI 464
Query: 407 PRSGKESNLDDITITDDSFKLRWPFEDDVPSDWERIDPELTRNTGIFYTGKMPYIAPDTK 466
S K+ L + +D+FKL++P + VP DWE++D ELT N IFYTGKMPYIA DTK
Sbjct: 465 NNSTKD-ELSPNFLNEDNFKLKYPMTEPVPRDWEKMDSELTDNLTIFYTGKMPYIAKDTK 523
Query: 467 FFPAALPDDGTMDFVLTDGRTPLTRIAPILMSLNRGSHVLQPEVEHSKILAFRLVPYAKN 526
FFPAALP DGT+D V+TD R P+TR+ PIL+SL++GSHVL+PEV HSKILA++++P ++
Sbjct: 524 FFPAALPADGTIDLVITDARIPVTRMTPILLSLDKGSHVLEPEVIHSKILAYKIIPKVES 583
Query: 527 SVISVDGENFPYETLQVEVLPKLVKTLLKNGSYVETDFESM 567
+ SVDGE FP E LQVE++P L KTLL+NG Y++T+FESM
Sbjct: 584 GLFSVDGEKFPLEPLQVEIMPMLCKTLLRNGRYIDTEFESM 624
>SPAC4A8.07c [IT] KOG1116 Sphingosine kinase involved in sphingolipid metabolism
Length = 458
Score = 207 bits (528), Expect = 3e-53
Identities = 147/437 (33%), Positives = 211/437 (47%), Gaps = 48/437 (10%)
Query: 121 WPDHTALIEVTFAKPRRNHLVPKTIKLSVDTSVFPNMGTDVAEEILNRSYKNTKRNKSIL 180
W D T VT + R+ V K VD F E +L+ +YK KR++ +
Sbjct: 55 WVDKTGPNSVTLSYVSRSSKV--ATKCWVD---FVENSDQFCEYLLDVAYKGIKRSRRFI 109
Query: 181 VIINPHGGKGKANKLFLSKAKPILLA--SGCKIVVKETTYHLHAVDIAKNLDIDEYDVVA 238
V INPHGGKGKA ++ S+A+P+ + S C++V+ T HA IAKNLD+ YD +
Sbjct: 110 VFINPHGGKGKAKHIWESEAEPVFSSAHSICEVVL--TRRKDHAKSIAKNLDVGSYDGIL 167
Query: 239 CASGDGIPHEIMNGLFLRSDRAKAFQKLAITQLPCGSGNAMSISCHGTSNPSYAALALLK 298
GDG+ HE++NGL R D +AF KL + +P GSGNA S + G P+ AL +LK
Sbjct: 168 SVGGDGLFHEVINGLGERDDYLEAF-KLPVCMIPGGSGNAFSYNATGQLKPALTALEILK 226
Query: 299 SVESRIDVMCCTQPSYKNQPRLSFLSQTYGIIAESDIDTEFIRFIGPARFELGVTMNVLQ 358
+ D+M Q K + SFL+ YGIIA+ DI TE RF+G R LG + + Q
Sbjct: 227 GRPTSFDLMTFEQ---KGKKAYSFLTANYGIIADCDIGTENWRFMGENRAYLGFFLRLFQ 283
Query: 359 RKKYPCDIYFKYAAKTKNELKDHYLQHKDRLNNLRNDLESSSTFTLDIPRSGKESNLDDI 418
+ + C I + + E+K Y + K+ L + +S T + + ES+L
Sbjct: 284 KPDWKCSIEMDVVSSDRTEIKHMYEKSKN-LAPMSESSDSDKTVS-----TSPESHLLTF 337
Query: 419 TITDDSFKLRWPFEDDVPSDWERIDPELTRNTGIFYTGKMPYIAPDTKFFPAALPDDGTM 478
I D S IF G +PYIAPD K FPAA DDG +
Sbjct: 338 EINDLS---------------------------IFCAGLLPYIAPDAKMFPAASNDDGLI 370
Query: 479 DFVLTDGRTPLTRIAPILMSLNRGSHVLQPEVEHSKILAFRLVPY--AKNSVISVDGENF 536
D V+ + + + L+ G + + K+ +FR P K ++DGE++
Sbjct: 371 DVVIVYSKQFRKSLLSMFTQLDNGGFYYSKHLNYYKVRSFRFTPVNTGKRHYFALDGESY 430
Query: 537 PYETLQVEVLPKLVKTL 553
P E + V PKL TL
Sbjct: 431 PLEPFECRVAPKLGTTL 447
>At4g21540 [IT] KOG1116 Sphingosine kinase involved in sphingolipid metabolism
Length = 1240
Score = 117 bits (292), Expect = 6e-26
Identities = 103/376 (27%), Positives = 169/376 (44%), Gaps = 37/376 (9%)
Query: 175 RNKSILVIINPHGGKGKANKLFLSKAKPILLASGCKIVVKETTYHLHAVDIAKNLDIDEY 234
R K +LV +NP GGK A ++F+ + KP+ + ++ ++ET Y LHA + K++D+ +Y
Sbjct: 376 RPKRLLVFVNPFGGKKSAREIFVKEVKPLFEDADVQLEIQETKYQLHAKEFVKSMDVSKY 435
Query: 235 DVVACASGDGIPHEIMNGLFLRSDRAKAFQKLAITQLPCGSGNAMSISCHGTSN----PS 290
D + C SGDGI E++NGL R+D A KL I +P G+GN M S T +
Sbjct: 436 DGIVCVSGDGILVEVVNGLLERADWRNAL-KLPIGMVPAGTGNGMIKSLLDTVGLRCCAN 494
Query: 291 YAALALLKSVESRIDVMCCTQPSYKNQPRLSFLSQTYGIIAESDIDTEFIRFIGPARFEL 350
A +++++ + +DV Q N S L +G+IA+ DI++E R++G AR +
Sbjct: 495 SATISIIRGHKRSVDVATIAQ---GNTKFFSVLMLAWGLIADIDIESEKFRWMGSARIDF 551
Query: 351 GVTMNVLQRKKYPCDIYFKYAAKTKNELKDHYLQHKDRLNNLRNDLESSSTFTLDIPRSG 410
V + D + Y L + R N L + P S
Sbjct: 552 YVCL---------VDKFDNYCIAVVKLLALQRIICLRRYNGRILFLPAPGFEGYGQPASC 602
Query: 411 KESNLDDITITDDSFKLRWPFEDDVPSDW-ERIDPELTRNTGIFYTGKMPYIAPDTKFFP 469
S + ++D + P +W E P +T + +P+ + +T P
Sbjct: 603 --SLYQEPHVSDKEVGYQGPETKFEDLEWREMKGPFVT-----IWLHNVPWGSENTLTAP 655
Query: 470 AALPDDGTMDFVLTDGRTPLTRIAPILMSLNRGSHVLQPEVEH-----SKILAFRLVPYA 524
AA DG +D ++ P + ++ + G+HV P + + K+ AF L P A
Sbjct: 656 AAKFSDGYLDLIVLK-NCPKLVLLSLMRQTSSGTHVESPYIVYIKLTVEKVKAFVLEPGA 714
Query: 525 ------KNSVISVDGE 534
K +I DGE
Sbjct: 715 LVDEPDKEGIIDSDGE 730
Score = 102 bits (253), Expect = 2e-21
Identities = 59/201 (29%), Positives = 101/201 (49%), Gaps = 31/201 (15%)
Query: 175 RNKSILVIINPHGGKGKANKLFLSKAKPILLASGCKIVVKETTYHLHAVDIAKNLDIDEY 234
R K +LV +NP GGK A K+F + KP+ + ++ ++ET Y LHA +I +++D+ +Y
Sbjct: 906 RPKKLLVFVNPFGGKKTARKIFQEEVKPLFEDANIQLEIQETKYQLHAKEIVRSMDVSKY 965
Query: 235 DVVACASGDGIPHEIMNGLFLRSDRAKAFQKLAITQLPCGSGNAMSISCHGTSNPSYAAL 294
D + C SGDGI E++NGL R D A KL I +P G ++ ++ + ++
Sbjct: 966 DGIVCVSGDGILVEVVNGLLEREDWKTAI-KLPIGMVPAGRTRSLDVATISQGTTKFFSV 1024
Query: 295 ALLKSVESRIDVMCCTQPSYKNQPRLSFLSQTYGIIAESDIDTEFIRFIGPARFELGVTM 354
+L +G++A+ DI++E R++G ARF++ V +
Sbjct: 1025 LML----------------------------AWGLVADIDIESEKFRWMGSARFDIYVCL 1056
Query: 355 NVLQRKKYPCDIYFKYAAKTK 375
++ K C+ F TK
Sbjct: 1057 --VEHLKIFCNACFNMRYSTK 1075
>HsM11464967 [IT] KOG1116 Sphingosine kinase involved in sphingolipid metabolism
Length = 384
Score = 103 bits (258), Expect = 5e-22
Identities = 90/382 (23%), Positives = 159/382 (41%), Gaps = 55/382 (14%)
Query: 179 ILVIINPHGGKGKANKLFLSKAKPILLASGCKIVVKETTYHLHAVDIAKNLDIDEYDVVA 238
+LV++NP GGKGKA +LF S +P+L + + T HA ++ ++ ++ +D +
Sbjct: 17 VLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALV 76
Query: 239 CASGDGIPHEIMNGLFLRSDRAKAFQKLAITQLPCGSGNAMSISCHGTSN---------P 289
SGDG+ HE++NGL R D A QK + LP GSGNA++ S + +
Sbjct: 77 VMSGDGLMHEVVNGLMERPDWETAIQK-PLCSLPAGSGNALAASLNHYAGYEQVTNEDLL 135
Query: 290 SYAALALLKSVESRIDVMCCTQPSYKNQPRLSFLSQTYGIIAESDIDTEFIRFIGPARFE 349
+ L L + + S ++++ S S LS +G IA+ D+++E R +G RF
Sbjct: 136 TNCTLLLCRRLLSPMNLLSLHTAS--GLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFT 193
Query: 350 LGVTMNVLQRKKYPCDIYFKYAAKTKNELKDHYLQHKDRLNNLRNDLESSSTFTLDIPRS 409
LG + + + Y + + L + R
Sbjct: 194 LGTFLRLAALRTYRGRLAY-----------------------------------LPVGRV 218
Query: 410 GKESNLDDITITDDSFKLRW-PFEDDVPSDWERI-DPELTRNTGIFYTGKMPYIAPDTKF 467
G ++ + + P E+ VPS W + D + + ++ ++ +
Sbjct: 219 GSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTMVPDEDFVLILALLHS----HLGSEMFA 274
Query: 468 FPAALPDDGTMDFVLTDGRTPLTRIAPILMSLNRGSHVLQ--PEVEHSKILAFRLVPYAK 525
P G M + +++ +G H+ P + + ++AFRL P
Sbjct: 275 APMGRCAAGVMHLFYVRAGVSRAMLLRFFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDG 334
Query: 526 NSVISVDGENFPYETLQVEVLP 547
V +VDGE E +Q +V P
Sbjct: 335 KGVFAVDGELMVSEAVQGQVHP 356
>Hs21361088 [IT] KOG1116 Sphingosine kinase involved in sphingolipid metabolism
Length = 398
Score = 103 bits (258), Expect = 5e-22
Identities = 90/382 (23%), Positives = 160/382 (41%), Gaps = 55/382 (14%)
Query: 179 ILVIINPHGGKGKANKLFLSKAKPILLASGCKIVVKETTYHLHAVDIAKNLDIDEYDVVA 238
+LV++NP GGKGKA +LF S +P+L + + T HA ++ ++ ++ +D +
Sbjct: 31 VLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALV 90
Query: 239 CASGDGIPHEIMNGLFLRSDRAKAFQKLAITQLPCGSGNAMSISCHGTSN---------P 289
SGDG+ HE++NGL R D A QK + LP GSGNA++ S + +
Sbjct: 91 VMSGDGLMHEVVNGLMERPDWETAIQK-PLCSLPAGSGNALAASLNHYAGYEQVTNEDLL 149
Query: 290 SYAALALLKSVESRIDVMCCTQPSYKNQPRLSFLSQTYGIIAESDIDTEFIRFIGPARFE 349
+ L L + + S ++++ S S LS +G IA+ D+++E R +G RF
Sbjct: 150 TNCTLLLCRRLLSPMNLLSLHTAS--GLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFT 207
Query: 350 LGVTMNVLQRKKYPCDIYFKYAAKTKNELKDHYLQHKDRLNNLRNDLESSSTFTLDIPRS 409
LG + + + Y + + L + R
Sbjct: 208 LGTFLRLAALRTYRGRLAY-----------------------------------LPVGRV 232
Query: 410 GKESNLDDITITDDSFKLRW-PFEDDVPSDWERI-DPELTRNTGIFYTGKMPYIAPDTKF 467
G ++ + + P E+ VPS W + D + + ++ ++ +
Sbjct: 233 GSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHS----HLGSEMFA 288
Query: 468 FPAALPDDGTMDFVLTDGRTPLTRIAPILMSLNRGSHVLQ--PEVEHSKILAFRLVPYAK 525
P G M + + +++ +G H+ P + + ++AFRL P
Sbjct: 289 APMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDG 348
Query: 526 NSVISVDGENFPYETLQVEVLP 547
V +VDGE E +Q +V P
Sbjct: 349 KGVFAVDGELMVSEAVQGQVHP 370
>HsM9910562 [IT] KOG1116 Sphingosine kinase involved in sphingolipid metabolism
Length = 618
Score = 98.2 bits (243), Expect = 3e-20
Identities = 61/197 (30%), Positives = 103/197 (51%), Gaps = 12/197 (6%)
Query: 175 RNKSILVIINPHGGKGKANKLFLSKAKPILLASGCKIVVKETTYHLHAVDIAKNLDIDEY 234
R +L+++NP GG+G A + + P++ +G + +T HA ++ + L + E+
Sbjct: 143 RPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLSFNLIQTERQNHARELVQGLSLSEW 202
Query: 235 DVVACASGDGIPHEIMNGLFLRSDRAKAFQKLAITQLPCGSGNAM--SISCHGTSNPSY- 291
D + SGDG+ HE++NGL R D +A K+ + LPCGSGNA+ +++ HG P+
Sbjct: 203 DGIVTVSGDGLLHEVLNGLLDRPDWEEAV-KMPVGILPCGSGNALAGAVNQHGGFEPALG 261
Query: 292 ------AALALLKSVESRIDVMCCTQPSYKNQPRLSFLSQTYGIIAESDIDTEFIRFIGP 345
+L L + +D++ T S SFLS +G +++ DI +E R +G
Sbjct: 262 LDLLLNCSLLLCRGGGHPLDLLSVTLAS--GSRCFSFLSVAWGFVSDVDIQSERFRALGS 319
Query: 346 ARFELGVTMNVLQRKKY 362
ARF LG + + Y
Sbjct: 320 ARFTLGTVLGLATLHTY 336
>Hs21361699 [IT] KOG1116 Sphingosine kinase involved in sphingolipid metabolism
Length = 654
Score = 98.2 bits (243), Expect = 3e-20
Identities = 61/197 (30%), Positives = 103/197 (51%), Gaps = 12/197 (6%)
Query: 175 RNKSILVIINPHGGKGKANKLFLSKAKPILLASGCKIVVKETTYHLHAVDIAKNLDIDEY 234
R +L+++NP GG+G A + + P++ +G + +T HA ++ + L + E+
Sbjct: 179 RPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLSFNLIQTERQNHARELVQGLSLSEW 238
Query: 235 DVVACASGDGIPHEIMNGLFLRSDRAKAFQKLAITQLPCGSGNAM--SISCHGTSNPSY- 291
D + SGDG+ HE++NGL R D +A K+ + LPCGSGNA+ +++ HG P+
Sbjct: 239 DGIVTVSGDGLLHEVLNGLLDRPDWEEAV-KMPVGILPCGSGNALAGAVNQHGGFEPALG 297
Query: 292 ------AALALLKSVESRIDVMCCTQPSYKNQPRLSFLSQTYGIIAESDIDTEFIRFIGP 345
+L L + +D++ T S SFLS +G +++ DI +E R +G
Sbjct: 298 LDLLLNCSLLLCRGGGHPLDLLSVTLAS--GSRCFSFLSVAWGFVSDVDIQSERFRALGS 355
Query: 346 ARFELGVTMNVLQRKKY 362
ARF LG + + Y
Sbjct: 356 ARFTLGTVLGLATLHTY 372
>7292298_1 [IT] KOG1116 Sphingosine kinase involved in sphingolipid metabolism
Length = 695
Score = 94.7 bits (234), Expect = 3e-19
Identities = 59/191 (30%), Positives = 100/191 (51%), Gaps = 5/191 (2%)
Query: 174 KRNKSILVIINPHGGKGKANKLFLSKAKPILLASGCKIVVKETTYHLHAVDIAKNLDIDE 233
+R + +LV++NP G G A ++F P+L + + T + A++ +D
Sbjct: 212 ERRRRVLVLLNPKSGSGDAREVFNMHVTPVLNEAEVPYDLYVTKHSNFAIEFLSTRCLDA 271
Query: 234 YDVVACASGDGIPHEIMNGLFLRSDRAKAFQKLAITQLPCGSGNAMSIS-CHGTSNPSY- 291
+ V GDG+ HEI+NGL R D A LA+ +PCGSGN ++ S H + P
Sbjct: 272 WCCVVAVGGDGLFHEIVNGLLQRQDWAHVLPHLALGIIPCGSGNGLARSIAHCYNKPVLG 331
Query: 292 AALALLKSVESRIDVMCCTQPSYKNQPRLSFLSQTYGIIAESDIDTEFIRFIGPARFELG 351
AAL ++ S +DV+ + +++ SFLS +G+I++ DI++E IR +G RF +
Sbjct: 332 AALTVISGRSSPMDVV---RVQLQSRSLYSFLSIGWGLISDVDIESERIRMLGYQRFTVW 388
Query: 352 VTMNVLQRKKY 362
++ + Y
Sbjct: 389 TLYRLVNLRTY 399
Score = 61.2 bits (147), Expect = 4e-09
Identities = 33/102 (32%), Positives = 55/102 (53%), Gaps = 3/102 (2%)
Query: 460 YIAPDTKFFPAALPDDGTMDFVLTDGRTPLTRIAPILMSLNRGSHVLQPEVEHSKIL--- 516
++ D F P A +DGT+ +L + L +++ G+H+ + +H K+L
Sbjct: 557 HLGIDCHFAPKAQLNDGTIYLILIRAGISRPHLLSFLYNMSSGTHLPESHDDHVKVLPVR 616
Query: 517 AFRLVPYAKNSVISVDGENFPYETLQVEVLPKLVKTLLKNGS 558
AFRL PY + +I+VDGE + LQ EVLP + + ++ N S
Sbjct: 617 AFRLEPYDNHGIITVDGERVEFGPLQAEVLPGIARVMVPNVS 658
>CE03053 [IT] KOG1116 Sphingosine kinase involved in sphingolipid metabolism
Length = 473
Score = 93.6 bits (231), Expect = 7e-19
Identities = 91/398 (22%), Positives = 172/398 (42%), Gaps = 57/398 (14%)
Query: 178 SILVIINPHGGKGKANKLFLSKAKPILLASGCKIVVKETTYHLHAVDIAKN-LDIDEYDV 236
++LV INP+ G GK+ + F + P L S + V TT HA ++ D+ +++
Sbjct: 87 NLLVFINPNSGTGKSLETFANTVGPKLDKSLIRYEVVVTTGPNHARNVLMTKADLGKFNG 146
Query: 237 VACASGDGIPHEIMNGLFLRSDRAKAFQKLAITQLPCGSGNAM---SISCHGT----SNP 289
V SGDG+ E +NG+ R D + F L I +P GSGN + +S +GT +
Sbjct: 147 VLILSGDGLVFEALNGILCREDAFRIFPTLPIGIVPSGSGNGLLCSVLSKYGTKMNEKSV 206
Query: 290 SYAALALLKSVESRIDVMCCTQPSYKNQPRLSFLSQTYGIIAESDIDTE----------- 338
AL + S ++ + + NQ SFLS +G++A+ DID+E
Sbjct: 207 MERALEIATSPTAKAESVALYSVKTDNQSYASFLSIGWGLMADIDIDSEKWRKSLGHHRF 266
Query: 339 ----FIRFIGPARFELGVTMNVLQRKKY--PCDIYFKYAAKTKNELKDHYLQHKDRLNNL 392
FIR ++ +T + K + +++ Y T+ + D ++ +++
Sbjct: 267 TVMGFIRSCNLRSYKGRLTYRPYKPKGFHPSSNVFSVYEKTTQQRIDDSKVKTNGSVSDS 326
Query: 393 RNDLESSSTFTLDIPRSGK-----ESNLDDITITDDSFKLRWPFEDDVPSDWERIDPELT 447
+ + +P S + S+L++ + +D+F
Sbjct: 327 EEETMETKFQNWTLPDSDETLAVGSSDLEETVVIEDNFV--------------------- 365
Query: 448 RNTGIFYTGKMPYIAPDTKFFPAALPDDGT--MDFVLTDGRTPLTRIAPILMSLNRGSHV 505
Y + +IA D F P+A +D + ++L IA L+++ +H+
Sbjct: 366 ----NIYAVTLSHIAADGPFAPSAKLEDNRIHLSYILWKDIGTRVNIAKYLLAIEHETHL 421
Query: 506 LQPEVEHSKILAFRLVPYAKNSVISVDGENFPYETLQV 543
P V+H ++ + +L ++ S + +DGE +T++V
Sbjct: 422 DLPFVKHVEVSSMKLEVISEGSHVVLDGEVVDTKTIEV 459
>7292647 [IT] KOG1116 Sphingosine kinase involved in sphingolipid metabolism
Length = 641
Score = 93.2 bits (230), Expect = 9e-19
Identities = 59/196 (30%), Positives = 104/196 (52%), Gaps = 10/196 (5%)
Query: 177 KSILVIINPHGGKGKANKLFLSKAKPILLASGCKIVVKETTYHLHAVDIAKNLD--IDEY 234
K +L+++NP G GK +LF + P+L + + ++ TT+ +A + + + Y
Sbjct: 189 KQLLILLNPKSGSGKGRELFQKQVAPLLTEAEVQYDLQITTHPQYAKEFVRTRRDLLTRY 248
Query: 235 DVVACASGDGIPHEIMNGLFLRSDRAKAFQKLAITQLPCGSGNAMSIS----CHGTSNPS 290
+ ASGDG+ +E++NGL R D +A ++L + +PCGSGN ++ S C+ P
Sbjct: 249 SGIVVASGDGLFYEVLNGLMERMDWRRACRELPLGIIPCGSGNGLAKSVAHHCNEPYEPK 308
Query: 291 ---YAALALLKSVESRIDVMCCTQPSY-KNQPRLSFLSQTYGIIAESDIDTEFIRFIGPA 346
+A L + + +DV+ + K+ SFLS +G+IA+ DI++E +R IG
Sbjct: 309 PILHATLTCMAGKSTPMDVVRVELATRDKHFVMYSFLSVGWGLIADIDIESERLRSIGAQ 368
Query: 347 RFELGVTMNVLQRKKY 362
RF L ++ + Y
Sbjct: 369 RFTLWAIKRLIGLRSY 384
Score = 51.6 bits (122), Expect = 3e-06
Identities = 28/101 (27%), Positives = 53/101 (51%), Gaps = 3/101 (2%)
Query: 460 YIAPDTKFFPAALPDDGTMDFVLTDGRTPLTRIAPILMSLNRGSHVLQPEVEHSKIL--- 516
+++ D F P + DDG + V+ ++ +++LN G+H+ E K++
Sbjct: 540 HLSSDVFFAPESRLDDGLIYLVIIRRGVSRHQLLNFMLNLNAGTHLPIGEDPFIKVVPCR 599
Query: 517 AFRLVPYAKNSVISVDGENFPYETLQVEVLPKLVKTLLKNG 557
AFR+ P + + ++ VDGE Y +Q EV+P L+ + +G
Sbjct: 600 AFRIEPSSSDGILVVDGERVEYGPIQAEVMPGLINVMTTSG 640
>At5g23450 [IT] KOG1116 Sphingosine kinase involved in sphingolipid metabolism
Length = 732
Score = 86.7 bits (213), Expect = 9e-17
Identities = 67/247 (27%), Positives = 121/247 (48%), Gaps = 5/247 (2%)
Query: 174 KRNKSILVIINPHGGKGKANKLFLSKAKPILLASGCKIVVKETTYHLHAVDIAKNLDIDE 233
K +LVI+NP G G++ K+F + +PI +G K+ V +TT HA ++A +DI+
Sbjct: 245 KSAPKMLVILNPRSGHGRSIKVFHNVVEPIFKLAGIKMEVVKTTKAGHARELASTVDINL 304
Query: 234 -YDVVACASGDGIPHEIMNGLFLRSDRAKAFQKLAITQLPCGSGNAMSISCHGTSNPSYA 292
D + C GDGI +E++NGL RS+ K + I +P GS N++ + G +P A
Sbjct: 305 CSDGIICVGGDGIINEVLNGLLTRSN-PKEGVSIPIGIVPAGSDNSLVWTVLGVRDPISA 363
Query: 293 ALALLKSVESRIDVMCCTQPSYKNQPRLSFLSQTYGIIAE-SDIDTEFIRFIGPARFELG 351
AL+++K + DV + + YG +++ ++ ++ + GP R+ +
Sbjct: 364 ALSIVKGGLTATDVF-AVEWIHTGIIHFGMTVSYYGFVSDVLELSEKYQKRFGPLRYFVA 422
Query: 352 VTMNVLQRKKYPCDIYFKYAAKTKNELKDHYLQHKDRLNNLRNDLESSSTFTLDIPRSGK 411
+ + KY ++ + A K E K + + +L D+ S+ PR+
Sbjct: 423 GFLKFMCLPKYSYEVEYLPAQKEDAEGKIRLEKEAVDMQDLYTDVMRRSS-REGFPRASS 481
Query: 412 ESNLDDI 418
S++D I
Sbjct: 482 LSSIDSI 488
>Hs20336726 [IT] KOG1115 Ceramide kinase
Length = 537
Score = 82.0 bits (201), Expect = 2e-15
Identities = 60/221 (27%), Positives = 99/221 (44%), Gaps = 13/221 (5%)
Query: 171 KNTKRNKSILVIINPHGGKGKANKLFLSKAKPILLASGCKIVVKETTYHLHAVDIAKNLD 230
K T R K +LV INP GGKG+ +++ K P+ + + T + A + ++
Sbjct: 125 KLTSRPKHLLVFINPFGGKGQGKRIYERKVAPLFTLASITTDIIVTEHANQAKETLYEIN 184
Query: 231 IDEYDVVACASGDGIPHEIMNGLFLRSDRAKAFQK-----------LAITQLPCGSGNAM 279
ID+YD + C GDG+ E+++GL R+ R+ + L I +P GS + +
Sbjct: 185 IDKYDGIVCVGGDGMFSEVLHGLIGRTQRSAGVDQNHPRAVLVPSSLRIGIIPAGSTDCV 244
Query: 280 SISCHGTSNPSYAALALLKSVESRIDVMCCTQPSYKNQPRLSFLSQTYGIIAESDIDTEF 339
S GTS+ +AL ++ +DV S + +S L YG + D+E
Sbjct: 245 CYSTVGTSDAETSALHIVVGDSLAMDVSSVHHNSTLLRYSVSLLG--YGFYGDIIKDSEK 302
Query: 340 IRFIGPARFELGVTMNVLQRKKYPCDIYFKYAAKTKNELKD 380
R++G AR++ L Y + F A T +D
Sbjct: 303 KRWLGLARYDFSGLKTFLSHHCYEGTVSFLPAQHTVGSPRD 343
>7296762 [IT] KOG1115 Ceramide kinase
Length = 596
Score = 80.5 bits (197), Expect = 6e-15
Identities = 54/211 (25%), Positives = 93/211 (43%), Gaps = 12/211 (5%)
Query: 166 LNRSYKNTKRNKSILVIINPHGGKGKANKLFLSKAKPILLASGCKIVVKETTYHLHAVDI 225
L+ S R + +LV INP+GG+ + + +PI +G T DI
Sbjct: 193 LHSSSPTRMRVRRLLVFINPYGGRKAGAQTYERHVRPIFQLAGVDATCITTQRANQVKDI 252
Query: 226 AKNLDIDEYDVVACASGDGIPHEIMNGLFLRSDRAKAFQK----------LAITQLPCGS 275
+ D+ YD V C GDG E++NGL R R + L + +P GS
Sbjct: 253 LLSHDLGVYDAVCCVGGDGTVAEVINGLIFRQMRELGLDEQRPPYIPRPALPVGVIPAGS 312
Query: 276 GNAMSISCHGTSNPSYAALALLKSVESRIDVMCCTQPSYKNQPRLSFLSQTYGIIAESDI 335
+ ++ S HGT++ AA+ ++ +DV C+ + ++ R +YG + +
Sbjct: 313 TDTIAYSMHGTADVRTAAIHVILGQHRGLDV--CSVSNGQSLLRFCASVLSYGYLGDVAA 370
Query: 336 DTEFIRFIGPARFELGVTMNVLQRKKYPCDI 366
+E R++GP R+E L + Y ++
Sbjct: 371 QSENYRWMGPRRYEYSGVKAFLNNRGYDAEL 401
>CE18241 [IT] KOG1115 Ceramide kinase
Length = 549
Score = 68.6 bits (166), Expect = 2e-11
Identities = 58/228 (25%), Positives = 102/228 (44%), Gaps = 19/228 (8%)
Query: 168 RSYKNTKRNKSILVIINPHGGKGKANKLFLSKAKPIL-LASGCKIVVKETTYHLHAVDIA 226
R KN R K+I++ INP GG GKA K+F L G + V T HA D
Sbjct: 158 RRVKN--RPKNIIIFINPFGGNGKAQKIFKDNVDAFFWLTPGLRYKVVLTERANHARDYI 215
Query: 227 KNLDIDEY---DVVACASGDGIPHEIMNGLFLRS--DRAKAFQK---------LAITQLP 272
+ +++ D + GDG+ +E+++G LR+ D + + +
Sbjct: 216 VEMPPEQWSAIDGLVSVGGDGLFNELLSGALLRTQTDAGRNIDNPSSHLVTPHIRFGIIG 275
Query: 273 CGSGNAMSISCHGTSNPSYAALALLKSVESRIDVMCCTQPSYKNQPRLSFLSQTYGIIAE 332
GS N++ + H T++ + +A+ + E +DV CT ++ R+S + +YG + +
Sbjct: 276 AGSANSIVSTVHETNDHATSAVHIAIGSECNVDV--CTVHQHQKLIRISANAISYGWLGD 333
Query: 333 SDIDTEFIRFIGPARFELGVTMNVLQRKKYPCDIYFKYAAKTKNELKD 380
D+E R +GP R++ ++ Y + F + K KD
Sbjct: 334 VLRDSEEYRCLGPIRYQWSALRTTIRHPIYRGMVQFSLSHKENVNPKD 381
>Hs8922701 [IT] KOG4435 Predicted lipid kinase
Length = 422
Score = 68.2 bits (165), Expect = 3e-11
Identities = 52/192 (27%), Positives = 87/192 (45%), Gaps = 12/192 (6%)
Query: 177 KSILVIINPHGGKGKANKLFLSKAKPILLASGCKIVVKETTYHLHAVDIAKNLDIDEYDV 236
K V +NP KGKA LF A PIL SG + + +T Y A + + + + DV
Sbjct: 61 KKATVFLNPAACKGKARTLFEKNAAPILHLSGMDVTIVKTDYEGQAKKLLELM--ENTDV 118
Query: 237 VACASGDGIPHEIMNGLFLRSDRAKAFQKLAITQLPCGSGNAMSISCHGTSNPSY----- 291
+ A GDG E++ G+ R+D A F K+ I +P G +++S + S
Sbjct: 119 IIVAGGDGTLQEVVTGVLRRTDEA-TFSKIPIGFIPLGETSSLSHTLFAESGNKVQHITD 177
Query: 292 AALALLKSVESRIDVMCCTQPSYKNQPRLSFLSQTYGIIAESDIDTEFIRFIGPARFELG 351
A LA++K +DV+ K QP + +G ++ + ++GP + +
Sbjct: 178 ATLAIVKGETVPLDVL--QIKGEKEQPVFAMTGLRWGSFRDAGVKVSKYWYLGPLKIKAA 235
Query: 352 VTMNVLQRKKYP 363
+ L K++P
Sbjct: 236 HFFSTL--KEWP 245
>At2g46090 [IT] KOG1116 Sphingosine kinase involved in sphingolipid metabolism
Length = 364
Score = 57.8 bits (138), Expect = 4e-08
Identities = 38/138 (27%), Positives = 64/138 (45%), Gaps = 9/138 (6%)
Query: 177 KSILVIINPHGGKGKANKLFLSKAKPIL---LASGCKIVVKETTYHLHAVDIAKNLDIDE 233
+ ++ ++NP G G+ K + K P L L C + T+ HA+DI + D
Sbjct: 52 RDLVFVVNPQGANGRTAKEW-KKLLPHLRSRLGKDCNVSELLTSGPSHAIDITREAIRDG 110
Query: 234 YDVVACASGDGIPHEIMNGLFLRSD-----RAKAFQKLAITQLPCGSGNAMSISCHGTSN 288
D V GDG HE++NG F +A + A+ +P G+G+ + + ++
Sbjct: 111 ADAVIAVGGDGTLHEVVNGFFWEGKPVGYLSGEASRSTALGLIPLGTGSDFARTFGWNND 170
Query: 289 PSYAALALLKSVESRIDV 306
P A + + + SRIDV
Sbjct: 171 PCEAVERIARGMRSRIDV 188
>At5g51290 [IT] KOG1115 Ceramide kinase
Length = 533
Score = 51.2 bits (121), Expect = 4e-06
Identities = 55/277 (19%), Positives = 107/277 (37%), Gaps = 66/277 (23%)
Query: 171 KNTKRNKSILVIINPHGGKGKANKLFLSKAKPILLAS-GCKIVVKETTYHLHAVDIA-KN 228
K +R +++LV ++P GKG +K++ + +K + A K++V E H V + +N
Sbjct: 157 KEVERPRNLLVFVHPKSGKGNGSKVWETVSKIFIRAKVNTKVIVTERAGHAFDVMASIQN 216
Query: 229 LDIDEYDVVACASGDGIPHEIMNGLFLR----------SDRAKAFQKLAITQLP------ 272
++ YD + GDG +EI+NG L SD + Q + +P
Sbjct: 217 KELHTYDGIIAVGGDGFFNEILNGYLLSRLKVPLPPSPSDSFNSVQSRGSSSVPEPGDEV 276
Query: 273 ------------------------------------------CGSGNAMSISCHGTSNPS 290
GS +A+ + G +P
Sbjct: 277 HETDQKEHYPLLPDSVQEVMNFRIEDPDHPFSSERPRFGLIPAGSTDAIVMCTTGARDPV 336
Query: 291 YAALALLKSVESRIDVMCC----TQPSYKNQPRLSFLSQ--TYGIIAESDIDTEFIRFIG 344
+AL ++ + +D M T + +P + + + YG + ++E R++G
Sbjct: 337 TSALHIILGRKLFLDAMQVVRWKTASTSTIEPYIRYAASFAGYGFYGDVISESEKYRWMG 396
Query: 345 PARFELGVTMNVLQRKKYPCDIYFKYAAKTKNELKDH 381
P R++ T L+ + Y ++ F+ A ++ H
Sbjct: 397 PKRYDYVGTKIFLKHRSYEAEVMFEEAESENSKASLH 433
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,220,386
Number of Sequences: 60738
Number of extensions: 1382452
Number of successful extensions: 3313
Number of sequences better than 1.0e-05: 18
Number of HSP's better than 0.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 3248
Number of HSP's gapped (non-prelim): 32
length of query: 567
length of database: 30,389,216
effective HSP length: 112
effective length of query: 455
effective length of database: 23,586,560
effective search space: 10731884800
effective search space used: 10731884800
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)