ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactVI5549   suspect: LH   A     KOG2162  RNA processing and modification                                Nonsense-mediated mRNA decay protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactVI5549 1929767 1927062 -902 
         (902 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDR206w [A] KOG2162 Nonsense-mediated mRNA decay protein              314   3e-85

>YDR206w [A] KOG2162 Nonsense-mediated mRNA decay protein
          Length = 884

 Score =  314 bits (805), Expect = 3e-85
 Identities = 207/716 (28%), Positives = 352/716 (48%), Gaps = 79/716 (11%)

Query: 8   DLQETFSGFQKQLQSVLETD-FFRDDSLMKGAIQMIHSKIMQMLLQSLIPEGLHQDITTD 66
           DL   F+G + QL S+L+++  F+D +L+ G +  +HSK+   +L S I     +    D
Sbjct: 10  DLPTAFNGIKSQLNSILKSNQLFQDYALLNGFLAFVHSKLNAAILTS-IESQCGKSFAAD 68

Query: 67  TGSKKSEIRSSDAHRILDLTWKHVHYPIFKYFQNWRNRNVAEGS----RPNYAGHRQLNS 122
             S      SS    ILD +W+ VHYPIFK+FQ WRN  + E      +  +   R++NS
Sbjct: 69  LDSFDQSSISS----ILDFSWESVHYPIFKWFQMWRNYILFEKENKKQQTKFIDFRKMNS 124

Query: 123 ILQKIFPQIHKLYYSTLELIFANYNLTALIPSDTRSKLNISTKKLNDDASNVLKPEDSFS 182
            + K F  +   Y + +  ++  Y+++ L+P      L +S  +   +  ++L  + +F+
Sbjct: 125 KMLKFFKTVQNFYVNVINTVYKKYDISVLLPKRIIQDLKLSDIENTTNVGDILAVK-TFN 183

Query: 183 IDCVMAS------QRCLLYIGCSQRYKIIMEHLSDRYQQADFQKPLRYLDIASTIVPSVG 236
               +A        RCLL++G + RYK ++E +S++Y  ++F+K L +  +AS ++PS G
Sbjct: 184 SSSPLAHLIPTLFHRCLLFLGTAYRYKTLLEEISNKYSISNFKKSLDFFRLASLVLPSAG 243

Query: 237 ETFLQRGICYTHTKNFGNAAYQFVRSSLSRLPSDAGIPNFTNLLGDPNGSLFKKLLNSLD 296
           ET+ Q G  +  T N G A + FV+  ++++PS   I NF  L+ D   SL + L  ++ 
Sbjct: 244 ETYSQAGAIFLQTGNLGIAVFNFVKGMMTKMPSPVSIKNFGALMVDNKSSLNRSLHTTIM 303

Query: 297 DLKVQETIKKRIINMEIMEFYILPLIGSHIFPQTWKNN------RHSDRLKHFQTLLFDK 350
           +  +QE+   R    EI+EFY L L GS   P +W+++       +  +L+H +  L++ 
Sbjct: 304 NTYLQESKGPRTPAKEILEFYFLGLFGSVWSPTSWRDDTKPNQLNNGIKLRHLENALYET 363

Query: 351 IEIRYIKNISMIFQDLILLIGSFHMYQMINGVSSNTTSSAATIMPNDKTLMXXXXXXXXX 410
           +  RY+ NI  IF +LI+ IG FH+      +   +  SA T+                 
Sbjct: 364 MSARYLNNIKTIFHNLIITIGGFHLL-----LKRRSDVSAKTL----------------- 401

Query: 411 XXXXXXXXXXXXXETKFLEFIFKFFTHLIDKVIMKEF-KNCEMFQYLAMARIMMCWIKSH 469
                        E  +L F FK+  H+++ ++ + + +N E+ + L M RI+ CWIK++
Sbjct: 402 -------KDLRSNELDYLNFAFKYIAHILNDIVKESWSENPEVSEILGMVRIINCWIKAN 454

Query: 470 KNVLKFAHRSTSFCQSMVNLTNELLSSNDLSISFEHLHRPTRDYFYEEDIMLKEFGPTKF 529
             VL+++  +  F  ++  L N+++            H P R Y++EED+M+K      F
Sbjct: 455 PMVLQYSQSNLEFVNALAYLINDIVKKKPSPSFSITEHIPKRTYWFEEDLMVKGLSFVNF 514

Query: 530 TLSDFND-EKLLSMDNLPDRLVGKSK--NKLTAKEEHSSRVQVLVYSNKKFLEKNCCGFK 586
            LSDF+D EK+L MD+  DRL+G     +KL+A  E   R+Q +V  + + L+ N CG +
Sbjct: 515 QLSDFDDYEKILEMDHSLDRLIGNPPLCDKLSASSEMLLRLQAVVNISSQLLQNNNCGVE 574

Query: 587 LDTEKKRYVHTAVKRVKANSLPNIAKPESVTKSFSIDGAGSGKIYPQKNGFSVAFTTREN 646
               K RY+              I   E+V  S       + K          A   R+N
Sbjct: 575 WSDNKSRYIFN----------KKIGFKETVKNSMKTSKQSNEK----------AKLQRKN 614

Query: 647 SNASGGSSMDMSTSPHYTEEAVKDNTTPANPVWNYSGSSAPQPPLSFNVTPSFSVS 702
             ++   S+ M+        +  D+    +P   YSGSS P  P +FNV PS +++
Sbjct: 615 KPSTTNGSISMADLERQMRSSSLDS---FSPTMGYSGSSVPMAPDTFNVKPSGTIT 667


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.318    0.132    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,410,174
Number of Sequences: 60738
Number of extensions: 2094749
Number of successful extensions: 6174
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 6163
Number of HSP's gapped (non-prelim): 2
length of query: 902
length of database: 30,389,216
effective HSP length: 115
effective length of query: 787
effective length of database: 23,404,346
effective search space: 18419220302
effective search space used: 18419220302
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)