ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI5669 good S KOG0308 Function unknown Conserved WD40 repeat-containing protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI5669 1966373 1969033 887
(887 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOL087c [S] KOG0308 Conserved WD40 repeat-containing protein 469 e-131
SPAC31A2.14 [S] KOG0308 Conserved WD40 repeat-containing protein 111 4e-24
Hs18874090 [S] KOG0308 Conserved WD40 repeat-containing protein 77 1e-13
>YOL087c [S] KOG0308 Conserved WD40 repeat-containing protein
Length = 1116
Score = 469 bits (1206), Expect = e-131
Identities = 356/1120 (31%), Positives = 520/1120 (45%), Gaps = 255/1120 (22%)
Query: 1 MSKYTLSFGLL-PLELDSDTKHLLPISSITNLD----STVITTGRDGNVVIE-------E 48
M++ T+S+GL+ P S H+LPI+ I D + +T+GRDG++++ E
Sbjct: 1 MNQLTVSYGLISPDYCTSQDAHILPITKILYPDIPGKNYFLTSGRDGSIILHKNTQLSNE 60
Query: 49 TQT----------KLAVHSDWISGIACLSAAK--------FLTVSHDFTICY---NWKTT 87
+T ++ VHSDW S + ++ F++VSHDF+I N + T
Sbjct: 61 PETAATTIKNDAIRMQVHSDWASDLIHVNMKNSDPSAGDTFISVSHDFSIVLISVNAQLT 120
Query: 88 IWNHKIVGYHADYVKGIALIGLE-----------------------------DMKCRYAT 118
W+ KI+G H DY+K I I E + T
Sbjct: 121 TWDKKIIGDHDDYIKCIVPIHYEMSNDYELEEQEGGPDNVHDGINNGIVVDEQNNFLFVT 180
Query: 119 VGLDKKLKIWELDLFEVENEEDVEAKLLGKFDNTTHHDAGSIYCLTATKDVIIFGDNE-- 176
GLD+K+K+W L + + A LL FDN +D GSIY ++ F DN+
Sbjct: 181 GGLDRKIKLWCLS-----SGPEKMATLLHTFDNAQSNDTGSIYSMSPIIPKYSFDDNQTS 235
Query: 177 -----------GRLSWYDINSYSVIRTIDDAAGVNXXXXXXXXXXXXXXSTSADGEIKLW 225
G L +Y + I +A N STS+DG I +W
Sbjct: 236 RPFDFVAGDCNGDLIFYSCKYRKEVIRIQNAHRTNIKVVRTLDDSTRLISTSSDGVINVW 295
Query: 226 NVE------SGKCLWTKQLSH-----AIWSVEGTLSKDLFLGDCMGNI-----TQLQGNQ 269
++ +G K++ +IW V+GT L+ GD GN+ + + +
Sbjct: 296 DLNCRHDQTTGALQLPKKIGSWSWDSSIWCVQGTSLDKLYFGDSQGNVMRANLSSYEDAK 355
Query: 270 ISVIYKP-----------------SDCDTKT---GGILAMAII-EDVLWFSRXXXXXXXX 308
++ I+KP S D K GGIL +A++ + L FS
Sbjct: 356 LTRIFKPDHHHHHHHHHEHEEQNISTTDAKVKKYGGILDIALLPNEKLLFSFCTDSNLNV 415
Query: 309 XXXXXXXXTVIEGGHALVRCSLLTNRRHVITENTSGELEKWDIVSCELVEKISRDEGNFD 368
+V EGG AL R SLLTNRRHVITENT G++++WDIVSCEL+ EG+FD
Sbjct: 416 LDLTNNHFSVNEGGFALTRSSLLTNRRHVITENTKGQMQRWDIVSCELLNTFDSSEGSFD 475
Query: 369 EVVRKLNPKEVLQHWCSVSIKRGILFVKINERFLNTEVYGSALTDYVIVNEDKVDEINLD 428
++V K KE+L HWC+VS+K G+LFVKIN +FL TEVYGSAL DY +VN EIN D
Sbjct: 476 DIVMKYTSKEILSHWCTVSVKVGMLFVKINPKFLKTEVYGSALKDYQVVNN---IEINSD 532
Query: 429 ERYNLGRIAVNSLLHGFIDYELAKDKKFREDISSTKKQTPPSTPNLLRRHNSMASDHTNP 488
ERYNLG+I +NSL + FI YE+ KDK R+ I S KK+ ++ L +NS
Sbjct: 533 ERYNLGKIVINSLFNEFISYEVQKDKLLRKKIFSLKKKDLTNSLTLDTGYNS-------- 584
Query: 489 DQHKDNKSIKEKRRLSIFTKFTQSNNT---TPQSSAPNTPNGIPDVPHTYHGIIEES--- 542
+ K+NK K K I + + N TP +SAP TP + +++ +
Sbjct: 585 ESKKNNKDKKRKSTFKISSTLSIGNTNSSGTPPNSAPATPVMAETIVLEEQPLLQSASDK 644
Query: 543 --EDEMGMMLPGPA------------------------------TALPTSSKPANL---- 566
+D + ++ P PA T L T +P +
Sbjct: 645 AIDDSLELVQPLPASKKPYFRTQSSGSLLSRKFKSFRSTSGRATTGLNTPEEPKGILPDT 704
Query: 567 --------TLTRSVTEQSIERSATPSSTFGFHNRSSRVKLETNSIDDLHSISSTGN---G 615
+++ + ATP S H KL S DL S ++T N
Sbjct: 705 PHVINDDSAFPQAINTTQQSKDATPESMLWNHPFKLEQKLSAISSQDLPS-NNTHNKLRS 763
Query: 616 EVNFKFNSKSFSKDLSERKPGLFLNEYIEEVYHAYLKQAPNSSSTFMKFGRRIPETLFIR 675
N + NS S + ++KP F+ + +E++ +Y +Q N+SS +R+P T I+
Sbjct: 764 SENSRANSTSTLEGNEKKKPE-FMPDLLEQIQESYKQQYMNTSS-LKYLTKRLPVTKIIK 821
Query: 676 DSTRPVIQIKSGCILTVNHWKEGSTGETIAFSTYLPPPTYTAN----------------- 718
S+ P+I++KS ++ V+ WKEGS G + FST L PP++ N
Sbjct: 822 ASSCPIIRVKSATLVLVHLWKEGSCGGRVLFSTLL-PPSHVDNETVSGGKENSKPPDDEE 880
Query: 719 --------------DLID--------NYHTYESLERHLPIWLGKILFKEDKVIKTYPKIT 756
DLID +E LE +LP W K LF++ K ++ PK+
Sbjct: 881 VDLQAVDDDKLGKYDLIDGELGSRLNRRQIFEQLEENLPYWFAKALFRDIKTVEEQPKLN 940
Query: 757 FIIQPWK----DEAHLSEETQQ---------------------------SPEAERLVVEH 785
F+I PW EA +E ++ SP ++
Sbjct: 941 FLIMPWSSVGGSEAAGNENKKKFISASDTTESSGNDSSDSSLGNGNEAVSPSTQQQFHNM 1000
Query: 786 KRSGFHKKSKPPESPLMLPKINDSNVRLTAANMIKAKKIKTYIVDRFESKTPEMKNKEDP 845
+ G K S+ +P LP+I+++NV+L A MI+ KKIK Y+ DRFE+KTPEMK K +P
Sbjct: 1001 LKFGRPKTSEQELNPTDLPRISEANVKLVAPGMIRVKKIKLYVADRFETKTPEMKAKMEP 1060
Query: 846 SEWLEILCKGTVLENDITLSAIRTLYWKSNTDIVLEYRRK 885
S WL++LC+G VL+ND+TL+ +RTLYWKS DIVLEYRRK
Sbjct: 1061 SLWLDLLCRGQVLDNDMTLNTVRTLYWKSQGDIVLEYRRK 1100
>SPAC31A2.14 [S] KOG0308 Conserved WD40 repeat-containing protein
Length = 962
Score = 111 bits (278), Expect = 4e-24
Identities = 149/631 (23%), Positives = 251/631 (39%), Gaps = 148/631 (23%)
Query: 318 VIEGGHALVRCSLLTNRRHVITENTSGELEKWDIVSCELVEKISRDEGNFDEVVRKLNPK 377
VI GG L++ +L +RRHV+TE+ G WDI++C+ + + E +F+++V+ L+
Sbjct: 403 VIHGGIGLLKYRMLGDRRHVLTEDAVGNKCLWDILACKQAGEFDKSE-DFEKIVQSLDTV 461
Query: 378 EVLQHWCSVSIKRGILFVKINE-RFLNTEVYGSALTDYVIVNEDKVDEINLDERYNLGRI 436
+ + W SV+ GIL V ++E +++ E+Y KVD + D+R NLG
Sbjct: 462 QAIPRWASVNCLLGILAVTLDENHYMDAEIYADECPLL------KVDSPS-DKRINLGVW 514
Query: 437 AVNSLLHGFIDYELAKDKKFREDI----SSTKKQ-------------TPPSTPNLLRRHN 479
+ +L FID EL +D KFR+++ S KKQ PS + LR +
Sbjct: 515 ILKNLFREFIDAELHRDLKFRQNLDVVRSEAKKQIEAQREEARKGNVNMPSALSPLRIRS 574
Query: 480 -------------SMASDHT------NPDQHKDNKSIKEKRRLSIFTKFTQSNN---TTP 517
S D++ P S++ ++T T +++
Sbjct: 575 RPSPLSLPPEPLLSPTIDYSATPFPLEPPPESPGPSLQIPSNNPVYTNLTDTDSMMGAPD 634
Query: 518 QSSAPNTPN----------GIPDVPHTYHGIIEESEDEMGMMLPGPATALPTSSKPANLT 567
S P N G P V T I + E M SSK +
Sbjct: 635 YFSIPARQNRNRKPHTEVVGSPTVVRTKEIIPPKVTREGSFMGRLKKLGRSKSSK----S 690
Query: 568 LTRSVTEQSIERSATPSSTFGFHNRSSRVKLETNSIDDLHSISSTGNGEVNFKFNSK--S 625
L + S+ER+A SSRV S+ ++S T N VN N++ +
Sbjct: 691 LQTDFMKASVERAA-----------SSRVFSTGTSVTSPQALSKT-NNTVNNAANTENNT 738
Query: 626 FSKDLSE----RKPGL-----FLNEYIEEVYHAYLK-QAPNSSSTFMKFGRRIPETLFIR 675
+KD + PG E IE+++ Y+ + ++ + MK P
Sbjct: 739 LAKDKQQTSEASSPGTPRELKTTGELIEDLHEQYVHFKDKDTVLSLMK-----PP----N 789
Query: 676 DSTRPVIQIKSGCILTVNHWKEGSTGETIAFSTYLPPPTYTANDLIDNYHTYESLERHLP 735
D T P++ + S + ++ E S + Y A+D+ + LE +P
Sbjct: 790 DDTFPMLNLSSQITVIIS---EESPEAGNSRDIYRSTLENMADDI-------DLLENIMP 839
Query: 736 IWLGKILFKEDKVIKTYPKITFIIQPWKDEAHLSEETQQSPEAERLVVEHKRSGFHKKSK 795
WLG++L + KT P + F +QP+
Sbjct: 840 FWLGRLLLLNEFPSKTAPTVNFTLQPFPGSG----------------------------- 870
Query: 796 PPESPLMLPKINDSNVRLTAANMIKAKKIKTYIVDRFESKTPEMKNKEDPSEWLEILCKG 855
LP I + N RL+A+ M++A+KI Y + + ++ + L+ CK
Sbjct: 871 -------LPLIVNENTRLSASAMLRAQKIMDYSYSKLSQQRKDVSS-------LQFRCKD 916
Query: 856 TVLENDITLSAIRTLYWKSNTDIVLEYRRKP 886
V+ +TL+ ++ W+S D+V Y P
Sbjct: 917 VVVTPKMTLATVKARIWRSGDDVVFHYDVAP 947
Score = 53.9 bits (128), Expect = 1e-06
Identities = 56/222 (25%), Positives = 100/222 (44%), Gaps = 19/222 (8%)
Query: 52 KLAVHSDWISGIACLSAAK-FLTVSHDFTICYNWKTTIWNHKI---VGYHADYVKGIALI 107
K+ HS W++ IA ++ ++ S D T+ WK + N +G H DYVK +++
Sbjct: 74 KIQAHSAWVNDIALTHDSEGVISCSSDSTVKL-WKPHVLNASCLSTIGEHTDYVKRVSIP 132
Query: 108 GLEDMKCRYATVGLDKKLKIWELDLFEVENEEDVEAKLLGKFDNTTHHDAGSIYCLTATK 167
A+ GLDK++ +W+ ++ +E + + L +G +Y L A
Sbjct: 133 KYSKSPL-VASGGLDKRIIVWDYNV----GQEVMRFEQLPDCSLVVGPRSG-VYSLAANN 186
Query: 168 DVIIFGDNEGRLSWYDINSYSVIRTIDDAAGV--NXXXXXXXXXXXXXXSTSADGEIKLW 225
++I G + + +D S + I D G N + S+D IKLW
Sbjct: 187 NIIANGGLQKDIQLWDCVSK---KRITDLVGHTDNVRDILISDDGRTILTASSDATIKLW 243
Query: 226 NVESGKCLWT-KQLSHAIWSV--EGTLSKDLFLGDCMGNITQ 264
++ + KCL++ S ++W++ E K + GD G IT+
Sbjct: 244 SLRAQKCLFSFIAHSDSVWALYSEHPDLKVFYAGDRSGLITR 285
>Hs18874090 [S] KOG0308 Conserved WD40 repeat-containing protein
Length = 677
Score = 77.0 bits (188), Expect = 1e-13
Identities = 82/382 (21%), Positives = 160/382 (41%), Gaps = 58/382 (15%)
Query: 56 HSDWISGIA-CLSAAKFLTVSHDFTICYNWKTTIWN-HK-----IVGYHADYVKGIALIG 108
H+DW++ I C + ++ S D T+ +WN HK + H DYVK +A
Sbjct: 74 HTDWVNDIVLCCNGKTLISASSDTTV------KVWNAHKGFCMSTLRTHKDYVKALAYAK 127
Query: 109 LEDMKCRYATVGLDKKLKIWE---LDLFEVENEEDVEAKLLGKFDNTTHHDAGSIYCLTA 165
+++ A+ GLD+++ +W+ L N + L G D SIY L
Sbjct: 128 DKEL---VASAGLDRQIFLWDVNTLTALTASNNTVTTSSLSGNKD--------SIYSLAM 176
Query: 166 TK--DVIIFGDNEGRLSWYDINSYSVIRTIDDAAGVNXXXXXXXXXXXXXXSTSADGEIK 223
+ +I+ G E L +D + + + + N S S+DG I+
Sbjct: 177 NQLGTIIVSGSTEKVLRVWDPRTCAKLMKLKGHTD-NVKALLLNRDGTQCLSGSSDGTIR 235
Query: 224 LWNVESGKCL----------WTKQLSHAIWSV-EGTLSKDLFLGDCMG-NITQLQGNQIS 271
LW++ +C+ W Q++ A V G + ++ D +I L + +
Sbjct: 236 LWSLGQQRCIATYRVHDEGVWALQVNDAFTHVYSGGRDRKIYCTDLRNPDIRVLICEEKA 295
Query: 272 VIYK----------PSDCDTKTGGILAMAIIEDVLWFSRXXXXXXXXXXXXXXXXT---- 317
+ K P+ T + ++ + F T
Sbjct: 296 PVLKMELDRSADPPPAIWVATTKSTVNKWTLKGIHNFRASGDYDNDCTNPITPLCTQPDQ 355
Query: 318 VIEGGHALVRCSLLTNRRHVITENTSGELEKWDIVSCELVEKISRDEGNFDEVVRKLNPK 377
VI+GG ++++C +L ++RH++T++T+ + WD++ VE + + + F++ ++K
Sbjct: 356 VIKGGASIIQCHILNDKRHILTKDTNNNVAYWDVLKACKVEDLGKVD--FEDEIKKRFKM 413
Query: 378 EVLQHWCSVSIKRGILFVKINE 399
+ +W SV +K G+L + ++E
Sbjct: 414 VYVPNWFSVDLKTGMLTITLDE 435
Score = 50.8 bits (120), Expect = 9e-06
Identities = 27/94 (28%), Positives = 49/94 (51%), Gaps = 18/94 (19%)
Query: 812 RLTAANMIKAKKIKTYIVDRF-----ESKTPEMKNKEDPSEW-------------LEILC 853
RL+A++M++ +K+ ++ ++ ES+T N E P E +E+LC
Sbjct: 584 RLSASDMLQVRKVMEHVYEKIINLDNESQTTSSSNNEKPGEQEKEEDIAVLAEEKIELLC 643
Query: 854 KGTVLENDITLSAIRTLYWKSNTDIVLEYRRKPT 887
+ VL+ ++ L ++ WKS D+ L YR+K T
Sbjct: 644 QDQVLDPNMDLRTVKHFIWKSGGDLTLHYRQKST 677
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.133 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,130,985
Number of Sequences: 60738
Number of extensions: 2436123
Number of successful extensions: 8668
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 8638
Number of HSP's gapped (non-prelim): 14
length of query: 887
length of database: 30,389,216
effective HSP length: 115
effective length of query: 772
effective length of database: 23,404,346
effective search space: 18068155112
effective search space used: 18068155112
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)