ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI5731 good U KOG3232 Intracellular trafficking, secretion, and vesicular transport Vacuolar assembly/sorting protein DID2
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI5731 1992956 1993567 204
(204 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKR035w-a [U] KOG3232 Vacuolar assembly/sorting protein DID2 248 3e-66
SPBC13G1.12 [U] KOG3232 Vacuolar assembly/sorting protein DID2 125 5e-29
Hs9966901 [U] KOG3232 Vacuolar assembly/sorting protein DID2 121 8e-28
CE20715 [U] KOG3232 Vacuolar assembly/sorting protein DID2 121 8e-28
7293876 [U] KOG3232 Vacuolar assembly/sorting protein DID2 120 1e-27
At1g73030 [U] KOG3232 Vacuolar assembly/sorting protein DID2 101 6e-22
At1g17730 [U] KOG3232 Vacuolar assembly/sorting protein DID2 100 2e-21
At2g06530 [U] KOG3230 Vacuolar assembly/sorting protein DID4 57 1e-08
7301433 [U] KOG3230 Vacuolar assembly/sorting protein DID4 55 7e-08
CE21987 [U] KOG3230 Vacuolar assembly/sorting protein DID4 54 1e-07
Hs7656922 [U] KOG3230 Vacuolar assembly/sorting protein DID4 53 3e-07
Hs18594914 [U] KOG3232 Vacuolar assembly/sorting protein DID2 52 4e-07
SPAC4F8.01 [U] KOG3230 Vacuolar assembly/sorting protein DID4 51 1e-06
Hs14721561 [U] KOG3231 Predicted assembly/vacuolar sorting protein 50 2e-06
At1g03950 [U] KOG3230 Vacuolar assembly/sorting protein DID4 49 6e-06
>YKR035w-a [U] KOG3232 Vacuolar assembly/sorting protein DID2
Length = 204
Score = 248 bits (634), Expect = 3e-66
Identities = 134/205 (65%), Positives = 156/205 (75%), Gaps = 2/205 (0%)
Query: 1 MSRNSM-NLENTLFQLKFTXXXXXXXXXXXXXXXXXXVNKLKKIINESEEIGKLYASNAI 59
MSRNS LENTLFQLKFT NKLK+ +NE+E+I ++YASNAI
Sbjct: 1 MSRNSAAGLENTLFQLKFTSKQLQKQANKASKEEKQETNKLKRALNENEDISRIYASNAI 60
Query: 60 RKRNERVQLLKLASRVDAVASRVQTAVTMRQVSTSMAQVCRGMDKALQSMNLQQITMVMD 119
RK+NER+QLLKLASRVD+VASRVQTAVTMRQVS SM QVC+GMDKALQ+MNLQQITM+MD
Sbjct: 61 RKKNERLQLLKLASRVDSVASRVQTAVTMRQVSASMGQVCKGMDKALQNMNLQQITMIMD 120
Query: 120 KFEQQFEDLDASVNVYEDMGXXXXXXXXXXXXXXELMNQVADENGLELKHSAALTDLPDV 179
KFEQQFEDLD SVNVYEDMG ELM++VADENG+ELK SA L ++P++
Sbjct: 121 KFEQQFEDLDTSVNVYEDMGVNSDAMLVDNDKVDELMSKVADENGMELKQSAKLDNVPEI 180
Query: 180 KEAPAAVVNEEKEDKLAERLRALRG 204
K A V++EKEDKLA+RLRALRG
Sbjct: 181 K-AKEVNVDDEKEDKLAQRLRALRG 204
>SPBC13G1.12 [U] KOG3232 Vacuolar assembly/sorting protein DID2
Length = 178
Score = 125 bits (313), Expect = 5e-29
Identities = 77/167 (46%), Positives = 99/167 (59%), Gaps = 13/167 (7%)
Query: 43 IINESEEIGKLYASNAIRKRNERVQLLKLASRVDAVASRVQTAVTMRQVSTSMAQVCRGM 102
I + EI ++YASNAIRK+ E + LLKL+SR+DAV+SR+QTAVTMR VS +MA V RGM
Sbjct: 18 ITKGNSEIARIYASNAIRKQQESLNLLKLSSRIDAVSSRLQTAVTMRAVSGNMAGVVRGM 77
Query: 103 DKALQSMNLQQITMVMDKFEQQFEDLDASVNVYEDMGXXXXXXXXXXXXXXELMNQVADE 162
D+A+++MNL+ I+ VMDKFE QF+D++ LM VADE
Sbjct: 78 DRAMKTMNLEMISQVMDKFEAQFDDVNVQTGYMNKAMGSVTAVDTPQEDVDLLMQTVADE 137
Query: 163 NGLELKH------SAALTDLPDVKEAPAAVVNEEKEDKLAERLRALR 203
GLE S +P PAA V ++D L ERLRALR
Sbjct: 138 AGLEFNQNMNNNLSVPAASVP----TPAAPV---EDDNLQERLRALR 177
>Hs9966901 [U] KOG3232 Vacuolar assembly/sorting protein DID2
Length = 196
Score = 121 bits (303), Expect = 8e-28
Identities = 73/198 (36%), Positives = 108/198 (53%), Gaps = 7/198 (3%)
Query: 8 LENTLFQLKFTXXXXXXXXXXXXXXXXXXVNKLKKIINESE-EIGKLYASNAIRKRNERV 66
+E LF LKF K+KK I + E+ +++A NAIR++N+ V
Sbjct: 1 MEKHLFNLKFAAKELSRSAKKCDKEEKAEKAKIKKAIQKGNMEVARIHAENAIRQKNQAV 60
Query: 67 QLLKLASRVDAVASRVQTAVTMRQVSTSMAQVCRGMDKALQSMNLQQITMVMDKFEQQFE 126
L++++RVDAVA+RVQTAVTM +V+ SMA V + MD L++MNL++I+ +MDKFE QFE
Sbjct: 61 NFLRMSARVDAVAARVQTAVTMGKVTKSMAGVVKSMDATLKTMNLEKISALMDKFEHQFE 120
Query: 127 DLDASVNVYEDMGXXXXXXXXXXXXXXELMNQVADENGLELKHSAALTDLPDVKEAPAAV 186
LD ED L+ ++ADE GL+L +LP +
Sbjct: 121 TLDVQTQQMEDTMSSTTTLTTPQNQVDMLLQEMADEAGLDLN-----MELPQGQTGSVGT 175
Query: 187 -VNEEKEDKLAERLRALR 203
V ++D+L++RL LR
Sbjct: 176 SVASAEQDELSQRLARLR 193
>CE20715 [U] KOG3232 Vacuolar assembly/sorting protein DID2
Length = 205
Score = 121 bits (303), Expect = 8e-28
Identities = 72/201 (35%), Positives = 112/201 (54%), Gaps = 4/201 (1%)
Query: 4 NSMNLENTLFQLKFTXXXXXXXXXXXXXXXXXXVNKLKKIINE-SEEIGKLYASNAIRKR 62
+SM LE LF LKF +KL I + ++E+ +++A NAIRK+
Sbjct: 6 SSMALEKHLFDLKFAAKQLEKNAQRCEKDEKVEKDKLTAAIKKGNKEVAQVHAENAIRKK 65
Query: 63 NERVQLLKLASRVDAVASRVQTAVTMRQVSTSMAQVCRGMDKALQSMNLQQITMVMDKFE 122
NE V +K+A+R+DAVA+RVQTA T ++V+ SM+ V + M+ A++SMNL+++ +MD+FE
Sbjct: 66 NEAVNYIKMAARIDAVAARVQTAATQKRVTASMSGVVKAMESAMKSMNLEKVQQLMDRFE 125
Query: 123 QQFEDLDASVNVYEDMGXXXXXXXXXXXXXXELMNQVADENGLELKHSAALTDLPDVKEA 182
+ FEDLD + E L+ + AD+ G+EL +++P
Sbjct: 126 RDFEDLDVTTKTMEKTMDGTTVLNAPKSQVDALIAEAADKAGIELNQELP-SNVPTALPT 184
Query: 183 PAAVVNEEKEDKLAERLRALR 203
V+E+K+ L ERL ALR
Sbjct: 185 GTQAVSEDKD--LTERLAALR 203
>7293876 [U] KOG3232 Vacuolar assembly/sorting protein DID2
Length = 198
Score = 120 bits (301), Expect = 1e-27
Identities = 73/197 (37%), Positives = 109/197 (55%), Gaps = 3/197 (1%)
Query: 8 LENTLFQLKFTXXXXXXXXXXXXXXXXXXVNKLKKIINESE-EIGKLYASNAIRKRNERV 66
+E LF LKF K KK I + ++ +++A NAIR++N+ V
Sbjct: 1 MEKHLFNLKFAVKELERNSKKCEKEEKLEKAKAKKAIQKGNMDVARIHAENAIRQKNQAV 60
Query: 67 QLLKLASRVDAVASRVQTAVTMRQVSTSMAQVCRGMDKALQSMNLQQITMVMDKFEQQFE 126
L++++RVDAVASRVQ+A+T R+V+ SMA V + MD A++ MNL++I+ +M+KFE QFE
Sbjct: 61 NYLRMSARVDAVASRVQSALTTRKVTGSMAGVVKAMDAAMKGMNLEKISSLMEKFESQFE 120
Query: 127 DLDASVNVYEDMGXXXXXXXXXXXXXXELMNQVADENGLELKHSAALTDLPDVKEAPAAV 186
DLD +V E L+ QVADE GLEL + L + A+
Sbjct: 121 DLDVQSSVMEGTMSDTVTTSVPQGDVDNLLQQVADEAGLEL--NMELPSGVQSQSVGAST 178
Query: 187 VNEEKEDKLAERLRALR 203
+++D+L +RL LR
Sbjct: 179 AVSQEQDELTQRLARLR 195
>At1g73030 [U] KOG3232 Vacuolar assembly/sorting protein DID2
Length = 203
Score = 101 bits (252), Expect = 6e-22
Identities = 66/203 (32%), Positives = 99/203 (48%), Gaps = 8/203 (3%)
Query: 4 NSMNLENTLFQLKFTXXXXXXXXXXXXXXXXXXVNKLKKIINESEEIG-KLYASNAIRKR 62
N+ L N +F LKFT K+KK I + G ++YA NAIRKR
Sbjct: 3 NTDKLMNQIFDLKFTSKSLQRQSRKCEKEEKAEKLKVKKAIEKGNMDGARIYAENAIRKR 62
Query: 63 NERVQLLKLASRVDAVASRVQTAVTMRQVSTSMAQVCRGMDKALQSMNLQQITMVMDKFE 122
+E++ L+LASR+DAV +R+ T M ++ SM + + ++ +L + NLQ+++ MD FE
Sbjct: 63 SEQMNYLRLASRLDAVVARLDTQAKMTTITKSMTNIVKSLESSLATGNLQKMSETMDSFE 122
Query: 123 QQFEDLDASVNVYEDMGXXXXXXXXXXXXXXELMNQVADENGLELKHSAALTDLPDVKEA 182
+QF +++ E+ LM QVAD+ GLE+ LP
Sbjct: 123 KQFVNMEVQAEFMENAMAGSTSLSTPEGEVNSLMQQVADDYGLEVS-----VGLPQPAGH 177
Query: 183 PAAVVNEEK--EDKLAERLRALR 203
EEK ED L+ RL L+
Sbjct: 178 AIPTKTEEKVDEDDLSRRLAELK 200
>At1g17730 [U] KOG3232 Vacuolar assembly/sorting protein DID2
Length = 203
Score = 100 bits (248), Expect = 2e-21
Identities = 63/201 (31%), Positives = 100/201 (49%), Gaps = 4/201 (1%)
Query: 4 NSMNLENTLFQLKFTXXXXXXXXXXXXXXXXXXVNKLKKIINESEEIG-KLYASNAIRKR 62
N+ L N +F+LKFT K+KK I + G ++YA NAIRKR
Sbjct: 3 NTDKLMNQIFELKFTSKSLQRQARKCEKEERSEKLKVKKAIEKGNMDGARIYAENAIRKR 62
Query: 63 NERVQLLKLASRVDAVASRVQTAVTMRQVSTSMAQVCRGMDKALQSMNLQQITMVMDKFE 122
+E++ L+L+SR+DAV +R+ T M ++ SM + + ++ +L + NLQ+++ MD FE
Sbjct: 63 SEQMNYLRLSSRLDAVVARLDTQAKMATITKSMTNIVKSLESSLTTGNLQKMSETMDSFE 122
Query: 123 QQFEDLDASVNVYEDMGXXXXXXXXXXXXXXELMNQVADENGLELKHSAALTDLPDVKEA 182
+QF +++ ++ LM QVAD+ GLE+ S L P
Sbjct: 123 KQFVNMEVQAEFMDNAMAGSTSLSTPEGEVNSLMQQVADDYGLEV--SVGLPQ-PAGHAI 179
Query: 183 PAAVVNEEKEDKLAERLRALR 203
P + +ED L RL L+
Sbjct: 180 PTKTEEKVEEDDLTRRLAELK 200
>At2g06530 [U] KOG3230 Vacuolar assembly/sorting protein DID4
Length = 218
Score = 57.4 bits (137), Expect = 1e-08
Identities = 41/157 (26%), Positives = 74/157 (47%), Gaps = 10/157 (6%)
Query: 41 KKIINESEEIGK--------LYASNAIRKRNERVQLLKLASRVDAVASRVQTAVTMRQVS 92
KK+INE ++ K + A + IR R++ + KL S++ V+ R+QT + + +
Sbjct: 35 KKLINEIKKTAKQGQMGAVKVMAKDLIRTRHQIEKFYKLKSQLQGVSLRIQTLKSTQAMG 94
Query: 93 TSMAQVCRGMDKALQSMNLQQITMVMDKFEQQFEDLDASVNVYEDMGXXXXXXXXXXXXX 152
+M V + M + + MNL + +M +FE+Q E ++ V D
Sbjct: 95 EAMKGVTKAMGQMNRQMNLPSLQKIMQEFERQNEKMEMVSEVMGDAIDDALEGDEEEEET 154
Query: 153 XELMNQVADENGLELKHSAALTDLPDVKEAPAAVVNE 189
+L++QV DE G+++ L + P A A N+
Sbjct: 155 EDLVSQVLDEIGIDINQE--LVNAPSGAVAVPAAKNK 189
>7301433 [U] KOG3230 Vacuolar assembly/sorting protein DID4
Length = 256
Score = 55.1 bits (131), Expect = 7e-08
Identities = 40/143 (27%), Positives = 70/143 (47%), Gaps = 11/143 (7%)
Query: 40 LKKIINESE-EIGKLYASNAIRKRNERVQLLKLASRVDAVASRVQTAVTMRQVSTSMAQV 98
+KK+ E + + K+ A + +R R + + + + + AV+ ++QT + +MAQ
Sbjct: 47 IKKMAKEGQMDAVKIMAKDLVRTRRYAKKFMLMKANIQAVSLKIQTL----KSQNTMAQA 102
Query: 99 CRGMDKALQSM----NLQQITMVMDKFEQQFEDLDASVNVYEDMGXXXXXXXXXXXXXXE 154
+G+ KA+Q+M NL QI ++ FE+Q E +D + D
Sbjct: 103 MKGVTKAMQNMNRQLNLPQIQKILQDFEKQSEMMDMKEEMINDAIDDAMEDEGDEEETDA 162
Query: 155 LMNQVADENGLELKHSAALTDLP 177
+++QV DE GL+L L DLP
Sbjct: 163 VVSQVLDELGLQL--GEQLGDLP 183
>CE21987 [U] KOG3230 Vacuolar assembly/sorting protein DID4
Length = 237
Score = 54.3 bits (129), Expect = 1e-07
Identities = 35/135 (25%), Positives = 66/135 (47%), Gaps = 3/135 (2%)
Query: 38 NKLKKIINESEEIGKLYASNAIRKRNERVQLLKLASRVDAVASRVQTAVTMRQVSTSMAQ 97
N KK N+ + + K+ A + +R R + + + + + AV+ +VQT + ++++M
Sbjct: 49 NMAKK--NQMDSV-KVMAKDLVRTRRYIKKFIVMKANIQAVSLKVQTLKSQDAMASAMKG 105
Query: 98 VCRGMDKALQSMNLQQITMVMDKFEQQFEDLDASVNVYEDMGXXXXXXXXXXXXXXELMN 157
V + M + +NL QI +M +FE+Q E +D V D +++N
Sbjct: 106 VTKAMQSMNRQLNLPQIQKIMMEFEKQSEIMDMKEEVMGDAIDDALGDAGDEEETDQIVN 165
Query: 158 QVADENGLELKHSAA 172
QV DE G+++ A
Sbjct: 166 QVLDELGIQMGEEMA 180
>Hs7656922 [U] KOG3230 Vacuolar assembly/sorting protein DID4
Length = 222
Score = 52.8 bits (125), Expect = 3e-07
Identities = 50/186 (26%), Positives = 85/186 (44%), Gaps = 29/186 (15%)
Query: 41 KKIINESEEIGK--------LYASNAIRKRNERVQLLKLASRVDAVASRVQTAVTMRQVS 92
KKII + +++ K + A + +R R + + + + + AV+ ++QT + +
Sbjct: 41 KKIIADIKKMAKQGQMDAVRIMAKDLVRTRRYVRKFVLMRANIQAVSLKIQTL----KSN 96
Query: 93 TSMAQVCRGMDKALQSMN----LQQITMVMDKFEQQFEDLDASVNVYEDMGXXXXXXXXX 148
SMAQ +G+ KA+ +MN L QI +M +FE+Q E +D + D
Sbjct: 97 NSMAQAMKGVTKAMGTMNRQLKLPQIQKIMMEFERQAEIMDMKEEMMNDAIDDAMGDEED 156
Query: 149 XXXXXELMNQVADENGLELKHSAALTDLPDV-----------KEAPAAVVNEEKEDKLAE 197
+++QV DE GL L + L++LP K AA + + L E
Sbjct: 157 EEESDAVVSQVLDELGLSL--TDELSNLPSTGGSLSVAAGGKKAEAAASALADADADLEE 214
Query: 198 RLRALR 203
RL+ LR
Sbjct: 215 RLKNLR 220
>Hs18594914 [U] KOG3232 Vacuolar assembly/sorting protein DID2
Length = 417
Score = 52.4 bits (124), Expect = 4e-07
Identities = 32/97 (32%), Positives = 48/97 (48%), Gaps = 23/97 (23%)
Query: 41 KKIINESEEIGKLYASNAIRKRNERVQLLKLASRVDAVASRVQTAVTMRQVSTSMAQVCR 100
K I ++E +++A NAI ++N+ + L++++RV AVA R Q AVTM + S
Sbjct: 233 KAIQKGNKEAARIHAENAIHQKNQAIDFLRMSARVGAVAVRGQNAVTMGRTSA------- 285
Query: 101 GMDKALQSMNLQQITMVMDKFEQQFEDLDASVNVYED 137
+MDKF+ QFE LD ED
Sbjct: 286 ----------------LMDKFKHQFETLDIQTQQMED 306
>SPAC4F8.01 [U] KOG3230 Vacuolar assembly/sorting protein DID4
Length = 141
Score = 51.2 bits (121), Expect = 1e-06
Identities = 38/144 (26%), Positives = 65/144 (44%), Gaps = 6/144 (4%)
Query: 59 IRKRNERVQLLKLASRVDAVASRVQTAVTMRQVSTSMAQVCRGMDKALQSMNLQQITMVM 118
+R RN R +++ +++ A++ R+QT T Q+ SM R + +SMN+ + +
Sbjct: 1 MRLRNSRKKMMNAKTQLQAISLRLQTMRTSEQMMQSMRGATRLLTGMNKSMNIPAMARIT 60
Query: 119 DKFEQQFEDLDASVNVYEDMGXXXXXXXXXXXXXXELMNQVADENGLELKHSAALTDLPD 178
+FE++ E ++ + D EL+N+V DE G++L LPD
Sbjct: 61 QQFERENEIMEQRQEMI-DENMDDALEEDDEEEADELVNKVLDEIGVDLSQG-----LPD 114
Query: 179 VKEAPAAVVNEEKEDKLAERLRAL 202
V + ED L RL L
Sbjct: 115 AATQIGTVPELKTEDNLQARLDEL 138
>Hs14721561 [U] KOG3231 Predicted assembly/vacuolar sorting protein
Length = 213
Score = 50.1 bits (118), Expect = 2e-06
Identities = 33/164 (20%), Positives = 77/164 (46%), Gaps = 7/164 (4%)
Query: 37 VNKLKKIINESEEIGKLYASNAIRKRNERVQLLKLASRVDAVASRVQTAVTMRQVSTSMA 96
+ K+ KI N+ E K+ A + R ++ + ++S+V +++++ + + +++ +M+
Sbjct: 46 IKKMAKIGNK--EACKVLAKQLVHLRKQKTRTFAVSSKVTSMSTQTKVMNSQMKMAGAMS 103
Query: 97 QVCRGMDKALQSMNLQQITMVMDKFEQQFEDLDASVNVYEDMGXXXXXXXXXXXXXXELM 156
+ M + M+ Q+ M F+++ ++ + + D +++
Sbjct: 104 TTAKTMQAVNKKMDPQKTLQTMQNFQKENMKMEMTEEMINDTLDDIFDGSDDEEESQDIV 163
Query: 157 NQVADENGLEL-----KHSAALTDLPDVKEAPAAVVNEEKEDKL 195
NQV DE G+E+ K +A LP + A + +EE E +L
Sbjct: 164 NQVLDEIGIEISGKMAKAPSAARSLPSASTSKATISDEEIERQL 207
>At1g03950 [U] KOG3230 Vacuolar assembly/sorting protein DID4
Length = 213
Score = 48.5 bits (114), Expect = 6e-06
Identities = 40/164 (24%), Positives = 68/164 (41%), Gaps = 14/164 (8%)
Query: 47 SEEIGKLYASNAIRKRNE-------RVQLLKLASRVDAVASRVQTAVTMRQVSTSMAQVC 99
+E K+ A IR R + R Q+ +A+ A+ + A M+ + +MA +
Sbjct: 57 NEGATKILARQLIRLRQQIANLQGSRAQMRGIATHTQAMHAHTSVAAGMQGATKAMAAMS 116
Query: 100 RGMDKALQSMNLQQITMVMDKFEQQFEDLDASVNVYEDMGXXXXXXXXXXXXXXELMNQV 159
+ MD A Q+ VM +F++Q +D + + D +L NQV
Sbjct: 117 KNMDPAKQAK-------VMREFQKQSAQMDMTTEMMSDSIDDALDNDEAEDETEDLTNQV 169
Query: 160 ADENGLELKHSAALTDLPDVKEAPAAVVNEEKEDKLAERLRALR 203
DE G+++ + + A V D+L +RL ALR
Sbjct: 170 LDEIGIDIASQLSSAPKGKIGGKKAEDVGSSGIDELEKRLAALR 213
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.313 0.127 0.323
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,686,712
Number of Sequences: 60738
Number of extensions: 222480
Number of successful extensions: 674
Number of sequences better than 1.0e-05: 15
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 658
Number of HSP's gapped (non-prelim): 15
length of query: 204
length of database: 30,389,216
effective HSP length: 101
effective length of query: 103
effective length of database: 24,254,678
effective search space: 2498231834
effective search space used: 2498231834
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)