ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI5889 good E KOG2448 Amino acid transport and metabolism Dihydroxy-acid dehydratase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI5889 2049586 2047838 -583
(583 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJR016c [E] KOG2448 Dihydroxy-acid dehydratase 983 0.0
SPAC17G8.06c [E] KOG2448 Dihydroxy-acid dehydratase 719 0.0
At3g23940 [E] KOG2448 Dihydroxy-acid dehydratase 627 e-179
>YJR016c [E] KOG2448 Dihydroxy-acid dehydratase
Length = 585
Score = 983 bits (2542), Expect = 0.0
Identities = 477/574 (83%), Positives = 525/574 (91%)
Query: 8 RRFSTTRNVGKKLNKYSHIITEPKDQGASQAMLYATGFKEDDFGKAQVGVGSCWWSGNPC 67
R+FSTTR V KKLNKYS+IITEPK QGASQAMLYATGFK++DF K QVGVGSCWWSGNPC
Sbjct: 11 RQFSTTRCVAKKLNKYSYIITEPKGQGASQAMLYATGFKKEDFKKPQVGVGSCWWSGNPC 70
Query: 68 NMHLMDFNNRITESVNKAGLKGMQFNTIGVSDGISMGTKGMRYSLQSREVIADSFETIMM 127
NMHL+D NNR ++S+ KAGLK MQFNTIGVSDGISMGTKGMRYSLQSRE+IADSFETIMM
Sbjct: 71 NMHLLDLNNRCSQSIEKAGLKAMQFNTIGVSDGISMGTKGMRYSLQSREIIADSFETIMM 130
Query: 128 AQHYDANIAIPSCDKNMPGVLMAMGRHNRPSIMVYGGTILPGSPTCGSAKIPKKIDVVSA 187
AQHYDANIAIPSCDKNMPGV+MAMGRHNRPSIMVYGGTILPG PTCGS+KI K ID+VSA
Sbjct: 131 AQHYDANIAIPSCDKNMPGVMMAMGRHNRPSIMVYGGTILPGHPTCGSSKISKNIDIVSA 190
Query: 188 FQSYGQYLSKEFTETERQDVVQHACPGPGSCGGMYTANTMASVAEVLGMTLPNSSSFPAV 247
FQSYG+Y+SK+FTE ER+DVV+HACPGPGSCGGMYTANTMAS AEVLG+T+PNSSSFPAV
Sbjct: 191 FQSYGEYISKQFTEEEREDVVEHACPGPGSCGGMYTANTMASAAEVLGLTIPNSSSFPAV 250
Query: 248 SKEKLNECDTIGEAIKKTMELGITPRDVMTKEAFENAMTYVIATGGSTNAVLHMVAVAHS 307
SKEKL ECD IGE IKKTMELGI PRD++TKEAFENA+TYV+ATGGSTNAVLH+VAVAHS
Sbjct: 251 SKEKLAECDNIGEYIKKTMELGILPRDILTKEAFENAITYVVATGGSTNAVLHLVAVAHS 310
Query: 308 AGVKITPDDFQRISDKTPLLGDFKPSGKYVMADLITVGGTQAVIKYLYEEGFLHGNTITI 367
AGVK++PDDFQRISD TPL+GDFKPSGKYVMADLI VGGTQ+VIKYLYE LHGNT+T+
Sbjct: 311 AGVKLSPDDFQRISDTTPLIGDFKPSGKYVMADLINVGGTQSVIKYLYENNMLHGNTMTV 370
Query: 368 SGDTLAERAAKAVSLKDGQDIIRPLTNPIKTSGHLQILYGSLAPGGSVGKITGKEGTYFK 427
+GDTLAERA KA SL +GQ+II+PL++PIK +GHLQILYGSLAPGG+VGKITGKEGTYFK
Sbjct: 371 TGDTLAERAKKAPSLPEGQEIIKPLSHPIKANGHLQILYGSLAPGGAVGKITGKEGTYFK 430
Query: 428 GKARVFNDEPGFISALENGEIKKGEKTVVIIRYXXXXXXXXXXXXLKPSSALMGYGLGKD 487
G+ARVF +E FI ALE GEIKKGEKTVV+IRY LKPSSALMGYGLGKD
Sbjct: 431 GRARVFEEEGAFIEALERGEIKKGEKTVVVIRYEGPRGAPGMPEMLKPSSALMGYGLGKD 490
Query: 488 VALLTDGRFSGGSHGFLIGHIVPEAAEGGPIGLVRDGDEIIIDADNNKIDLLVPEEEIAE 547
VALLTDGRFSGGSHGFLIGHIVPEAAEGGPIGLVRDGDEIIIDADNNKIDLLV ++E+A+
Sbjct: 491 VALLTDGRFSGGSHGFLIGHIVPEAAEGGPIGLVRDGDEIIIDADNNKIDLLVSDKEMAQ 550
Query: 548 RRSTWTAPPPRYTRGCLSKYAKLVSNASHGCVTD 581
R+ +W APPPRYTRG LSKYAKLVSNAS+GCV D
Sbjct: 551 RKQSWVAPPPRYTRGTLSKYAKLVSNASNGCVLD 584
>SPAC17G8.06c [E] KOG2448 Dihydroxy-acid dehydratase
Length = 598
Score = 719 bits (1855), Expect = 0.0
Identities = 359/571 (62%), Positives = 426/571 (73%), Gaps = 3/571 (0%)
Query: 11 STTRNVGKKLNKYSHIITEPKDQGASQAMLYATGFKEDDFGKAQVGVGSCWWSGNPCNMH 70
S+ V KKLNKYS IT PK QGASQAMLYATG E+D K QVG+ SCW+ GNPCNMH
Sbjct: 28 SSINLVEKKLNKYSETITGPKSQGASQAMLYATGLNEEDMKKPQVGIASCWYEGNPCNMH 87
Query: 71 LMDFNNRITESVNKAGLKGMQFNTIGVSDGISMGTKGMRYSLQSREVIADSFETIMMAQH 130
L+D R+ E V KAGL G QFNTIGVSDGISMGT GMRYSLQSRE+IADS ET+M Q
Sbjct: 88 LLDLGRRVKEGVKKAGLTGFQFNTIGVSDGISMGTTGMRYSLQSREIIADSIETVMQGQW 147
Query: 131 YDANIAIPSCDKNMPGVLMAMGRHNRPSIMVYGGTILPGSPTCGSAKIPKKIDVVSAFQS 190
YDAN++IP CDKNMPG L+AMGR NRPSIMVYGG+I G C + ID+VSAFQS
Sbjct: 148 YDANVSIPGCDKNMPGCLIAMGRFNRPSIMVYGGSIRAGHSPCQNNA---PIDIVSAFQS 204
Query: 191 YGQYLSKEFTETERQDVVQHACPGPGSCGGMYTANTMASVAEVLGMTLPNSSSFPAVSKE 250
YG++++ + E R D+++HACPG G+CGGMYTANTMAS AE +GMTLP SSS+ A S E
Sbjct: 205 YGEFITGKIDEPTRHDIIRHACPGGGACGGMYTANTMASCAEAMGMTLPGSSSYLAGSPE 264
Query: 251 KLNECDTIGEAIKKTMELGITPRDVMTKEAFENAMTYVIATGGSTNAVLHMVAVAHSAGV 310
K EC+ G AIK+ + I PRD+MT+ AFENAM + GGSTN+VLH++A+A S G+
Sbjct: 265 KFAECEAAGSAIKRLLVDDIKPRDIMTRSAFENAMVLTMTLGGSTNSVLHLIAIAKSVGI 324
Query: 311 KITPDDFQRISDKTPLLGDFKPSGKYVMADLITVGGTQAVIKYLYEEGFLHGNTITISGD 370
+T DDFQ +S++TP + D KPSGKYVM DL +GG +V+KYL+ EG + G+ IT++G
Sbjct: 325 TLTLDDFQAVSNRTPFIADMKPSGKYVMEDLFAIGGIPSVLKYLHAEGLIDGSNITVTGK 384
Query: 371 TLAERAAKAVSLKDGQDIIRPLTNPIKTSGHLQILYGSLAPGGSVGKITGKEGTYFKGKA 430
TLAE L +GQ IIRPL+NPIKT GHL++L GSLAP GSV KITGKEG F GKA
Sbjct: 385 TLAENLRGFKDLAEGQKIIRPLSNPIKTEGHLRVLRGSLAPEGSVAKITGKEGLNFTGKA 444
Query: 431 RVFNDEPGFISALENGEIKKGEKTVVIIRYXXXXXXXXXXXXLKPSSALMGYGLGKDVAL 490
RVF+ E FI+ALE GE KKGEKTVVIIR+ LKPSSA+MG GLGKDVAL
Sbjct: 445 RVFDAENDFIAALERGEFKKGEKTVVIIRFEGPKGGPGMPEMLKPSSAIMGAGLGKDVAL 504
Query: 491 LTDGRFSGGSHGFLIGHIVPEAAEGGPIGLVRDGDEIIIDADNNKIDLLVPEEEIAERRS 550
LTDGRFSGGSHGFLIGH+ PEA GGPI LV+DGD I I+A N +DL+V E+E+A RRS
Sbjct: 505 LTDGRFSGGSHGFLIGHVDPEAQVGGPIALVQDGDVIEINAVKNTLDLMVDEKEMARRRS 564
Query: 551 TWTAPPPRYTRGCLSKYAKLVSNASHGCVTD 581
W APP +Y +G L KYA+ VS AS G VTD
Sbjct: 565 VWKAPPLKYQQGTLLKYARNVSTASKGAVTD 595
>At3g23940 [E] KOG2448 Dihydroxy-acid dehydratase
Length = 608
Score = 627 bits (1616), Expect = e-179
Identities = 311/563 (55%), Positives = 398/563 (70%), Gaps = 7/563 (1%)
Query: 19 KLNKYSHIITEPKDQGASQAMLYATGFKEDDFGKAQVGVGSCWWSGNPCNMHLMDFNNRI 78
KLNKYS ITEPK QG SQA+L+ G +DD K Q+G+ S W+ GN CNMHL+ + +
Sbjct: 52 KLNKYSSRITEPKSQGGSQAILHGVGLSDDDLLKPQIGISSVWYEGNTCNMHLLKLSEAV 111
Query: 79 TESVNKAGLKGMQFNTIGVSDGISMGTKGMRYSLQSREVIADSFETIMMAQHYDANIAIP 138
E V AG+ G +FNTIGVSD ISMGT+GM +SLQSR++IADS ET+M AQ YD NI+IP
Sbjct: 112 KEGVENAGMVGFRFNTIGVSDAISMGTRGMCFSLQSRDLIADSIETVMSAQWYDGNISIP 171
Query: 139 SCDKNMPGVLMAMGRHNRPSIMVYGGTILPGSPTCGSAKIPKKIDVVSAFQSYGQYLSKE 198
CDKNMPG +MAMGR NRP IMVYGGTI PG K D+VSAFQSYG+++S
Sbjct: 172 GCDKNMPGTIMAMGRLNRPGIMVYGGTIKPGHFQ------DKTYDIVSAFQSYGEFVSGS 225
Query: 199 FTETERQDVVQHACPGPGSCGGMYTANTMASVAEVLGMTLPNSSSFPAVSKEKLNECDTI 258
++ +R+ V+ H+CPG G+CGGMYTANTMAS E +GM+LP SSS PA KL+EC
Sbjct: 226 ISDEQRKTVLHHSCPGAGACGGMYTANTMASAIEAMGMSLPYSSSIPAEDPLKLDECRLA 285
Query: 259 GEAIKKTMELGITPRDVMTKEAFENAMTYVIATGGSTNAVLHMVAVAHSAGVKITPDDFQ 318
G+ + + +++ + PRD++T ++ NAM V+A GGSTNAVLH++A+A S G+++T DDFQ
Sbjct: 286 GKYLLELLKMDLKPRDIITPKSLRNAMVSVMALGGSTNAVLHLIAIARSVGLELTLDDFQ 345
Query: 319 RISDKTPLLGDFKPSGKYVMADLITVGGTQAVIKYLYEEGFLHGNTITISGDTLAERAAK 378
++SD P L D KPSGKYVM D+ +GGT AV++YL E G + G+ +T++G TLA+
Sbjct: 346 KVSDAVPFLADLKPSGKYVMEDIHKIGGTPAVLRYLLELGLMDGDCMTVTGQTLAQNLEN 405
Query: 379 AVSLKDGQDIIRPLTNPIKTSGHLQILYGSLAPGGSVGKITGKEGTYFKGKARVFNDEPG 438
SL +GQ+IIRPL+NPIK +GH+QIL G LAP GSV KITGKEG YF G A VF E
Sbjct: 406 VPSLTEGQEIIRPLSNPIKETGHIQILRGDLAPDGSVAKITGKEGLYFSGPALVFEGEES 465
Query: 439 FISALENGEIKKGEKTVVIIRYXXXXXXXXXXXXLKPSSALMGYGLGKDVALLTDGRFSG 498
++A+ + + + TVV+IR L P+SA+MG GLGK+ ALLTDGRFSG
Sbjct: 466 MLAAI-SADPMSFKGTVVVIRGEGPKGGPGMPEMLTPTSAIMGAGLGKECALLTDGRFSG 524
Query: 499 GSHGFLIGHIVPEAAEGGPIGLVRDGDEIIIDADNNKIDLLVPEEEIAERRSTWTAPPPR 558
GSHGF++GHI PEA EGGPIGL+++GD I ID +ID V EE+ +RR WTAP +
Sbjct: 525 GSHGFVVGHICPEAQEGGPIGLIKNGDIITIDIGKKRIDTQVSPEEMNDRRKKWTAPAYK 584
Query: 559 YTRGCLSKYAKLVSNASHGCVTD 581
RG L KY K V +AS GCVTD
Sbjct: 585 VNRGVLYKYIKNVQSASDGCVTD 607
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.135 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,153,072
Number of Sequences: 60738
Number of extensions: 1537828
Number of successful extensions: 3461
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3455
Number of HSP's gapped (non-prelim): 3
length of query: 583
length of database: 30,389,216
effective HSP length: 112
effective length of query: 471
effective length of database: 23,586,560
effective search space: 11109269760
effective search space used: 11109269760
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)