ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI6009 good D KOG4438 Cell cycle control, cell division, chromosome partitioning Centromere-associated protein NUF2
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI6009 2098204 2099556 451
(451 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOL069w [D] KOG4438 Centromere-associated protein NUF2 413 e-115
SPAC27F1.04c [D] KOG4438 Centromere-associated protein NUF2 122 8e-28
ECU09g1690 [D] KOG4438 Centromere-associated protein NUF2 69 2e-11
At1g61000_1 [D] KOG4438 Centromere-associated protein NUF2 61 3e-09
Hs7661992 [KRT] KOG4572 Predicted DNA-binding transcription fact... 50 7e-06
>YOL069w [D] KOG4438 Centromere-associated protein NUF2
Length = 451
Score = 413 bits (1062), Expect = e-115
Identities = 209/447 (46%), Positives = 303/447 (67%), Gaps = 2/447 (0%)
Query: 2 SKDVFPLLELSELVTCLQSCDFSLAVEENISKPSSQYVITLYKQIIDTFMGVSPDTLLSN 61
++DVFP+L+L ELV CLQSCDF+LA +ENIS+P+S Y++TLYKQII+ FMG+S ++LL++
Sbjct: 4 NQDVFPILDLQELVICLQSCDFALATQENISRPTSDYMVTLYKQIIENFMGISVESLLNS 63
Query: 62 EALFDNSGSNDIQQNPAYTETVKVLALNKICFKFFQDIGVSDFNMMDLYKPDSLRTRRLL 121
G + Y +T+ VL LNKICFKFF++IGV DFNM DLYKP++ RT+RLL
Sbjct: 64 SNQETGDGHLQEENENIYLDTLNVLVLNKICFKFFENIGVQDFNMTDLYKPEAQRTQRLL 123
Query: 122 SAVVNYARFREERMFDCDKFMSKTEFLLNQLRSKFDDYNYLQQQINKHR--NEIELREGE 179
SAVVNYARFREERMFDC+ F+ + E LL QLRSKFDDYN +QQQ+ ++ + + + +
Sbjct: 124 SAVVNYARFREERMFDCNSFILQMESLLGQLRSKFDDYNLIQQQLKQYEDVDGDNIPDEQ 183
Query: 180 TFETLQQQNKHLDQQISRLKGLQETLNIDYNAYKSRKQSLLHDLEKFGFELIELEFERNK 239
+ L++QNK L+ Q+ +L +QETL+IDYN YK KQS+ DLE F+++ELE R+K
Sbjct: 184 ELQKLEEQNKELEIQLKKLTKIQETLSIDYNDYKISKQSIFKDLEALSFQIVELESNRDK 243
Query: 240 LEKHSKTDFNELNLNIEKXXXXXXXXXXXXXXXXXKHRKLRISMNTFQTLTHELYDVLQL 299
L K S TD EL+ I++ + + L+ ++ TF+T+ ELYDVL++
Sbjct: 244 LIKISNTDMEELSEGIKELNDLLIQRKKTLDDLTAQQKNLQDTVTTFETIISELYDVLRI 303
Query: 300 ISTDLQESHLREANLIDMREQLLQTDQKLNNILSSGVMVKMKILQSQLDNQKKKLHQLEG 359
IS+++QES+ E L+ +++ L+ KL N+L +G+M K++ILQ QLD Q K L +L
Sbjct: 304 ISSEVQESNRTETELVGLKQNLINNKLKLMNVLETGIMYKLEILQEQLDLQLKNLEKLSQ 363
Query: 360 ETKAKQIENSETLQLLQRQYTDEVVPDLRAAEEQVENDLANGTIKNYDYEISQLKQAFQT 419
+TK + N L LQ +Y +E+ P + + ++ +L +G I + EI QL++ F+
Sbjct: 364 DTKEESRLNDTKLMDLQIKYENEIKPKIDKTDIFIQEELISGKINKLNDEIKQLQKDFEV 423
Query: 420 EIDAIDLEYSLLSSHINRYMEEMLRVM 446
E+ I++EYSLLS HIN+YM EML M
Sbjct: 424 EVKEIEIEYSLLSGHINKYMNEMLEYM 450
>SPAC27F1.04c [D] KOG4438 Centromere-associated protein NUF2
Length = 441
Score = 122 bits (307), Expect = 8e-28
Identities = 113/459 (24%), Positives = 210/459 (45%), Gaps = 45/459 (9%)
Query: 3 KDVFPLLELSELVTCLQSCDFSLAVEENISKPSSQYVITLYKQIIDTFMGVSPDTLLSNE 62
K FP L+ +E++ C+ +E + +P+S+ VI LY++ +D FMG++ L
Sbjct: 4 KHTFPSLKRAEILECIDGLGIPFTAKE-LDQPTSKAVIPLYEEFLDLFMGLTRQNLEEKV 62
Query: 63 ALFDNSGSNDIQQNPAYTETVKVLALNKICFKFFQDIGVSDFNMMDLYKPDSLRTRRLLS 122
NS + ++ E+++ +I +F Q+I DF + DL KPD R + +LS
Sbjct: 63 ----NSLQDSVENFEIIHESLRFTVFYQILSQFMQNICFHDFTIQDLLKPDRNRLQLILS 118
Query: 123 AVVNYARFREERMFDCDKFMSKTEFLLN-----QLRSKFDDYNYLQQQINKHRNEI---- 173
AV+N+A+ REER+ D + K E LL + K + L Q K +E
Sbjct: 119 AVINFAKLREERLQQFDDDIQKRESLLETYTLLDAQRKDLEEKVLLSQDRKLESEAIIKQ 178
Query: 174 -ELREGETFETLQQQNKHLDQ---QISRLKGLQETLNIDYNAYKSRKQSLLHDLEKFGFE 229
E R E F++L + + Q + R++ L I Y+ S S L ++EK
Sbjct: 179 NEERNEEMFQSLIEDKRLCSQVRTEYDRIRMEASELKIRYHNVDSLMASTLEEIEKLQSS 238
Query: 230 LIEL-EFERNKLEKHSKTDFNELNLNIEKXXXXXXXXXXXXXXXXXKHRKLRISMNTFQT 288
++ E + K+ S N+ + +E K + L +N+ Q
Sbjct: 239 IVHSPEKLKGKIADTSLRIQNDRSQQVE---------------LDKKSKILHTKLNSLQL 283
Query: 289 LTHELYDVLQLISTDLQESHLREANLIDMREQLLQTDQKLNN---ILSSGVMVKMKILQS 345
+ +L L+++ E L E + ++ LL T+Q+L + I + + + L
Sbjct: 284 IEGDLNACLKVL-----EECLVELDKLEHATVLLSTNQELCDQIEINKKKLEFRKEQLLK 338
Query: 346 QLDNQKKKLHQLEGETKAKQIENSETLQLLQRQYTDEVVPDLRAAEEQVENDLANGTIKN 405
QL N ++KL + K + + ++ +Y +V+ R + Q E + N I+
Sbjct: 339 QLSNAQEKLEHEQHSRNQKLEAAKQRMDNIREEY--KVITQERNKKIQ-ETEKKNAMIEM 395
Query: 406 YDYEISQLKQAFQTEIDAIDLEYSLLSSHINRYMEEMLR 444
+ +I+ +++ +++I +I +E+ L SH+ Y+ E+LR
Sbjct: 396 TEQKIAGMREELESQISSITMEFEKLKSHVELYIAELLR 434
>ECU09g1690 [D] KOG4438 Centromere-associated protein NUF2
Length = 439
Score = 68.6 bits (166), Expect = 2e-11
Identities = 90/448 (20%), Positives = 187/448 (41%), Gaps = 42/448 (9%)
Query: 7 PLLELSELVTCLQSCDFSLAVEENISKPSSQYVITLYKQIIDTFMGVSPDTLLSNEALFD 66
P L + E++ + ++ + I KP+ Q +Y+ +++ + GV LL
Sbjct: 10 PDLPVKEIMQYFSEMEINIKASD-ILKPTPQSTQRIYEVLLEVYCGVKTSDLLPRI---- 64
Query: 67 NSGSNDIQQNPAYTETVKVLALNKICFKFFQDIGVSDFNMMDLYKPDSLRTRRLLSAVVN 126
NSG + A+ E++ + L K F + IG+ +F + DL PDS R +LS VVN
Sbjct: 65 NSG----ESIEAFEESLSCILLQKRMSGFLKRIGIDNFGLRDLV-PDSRRLIGILSVVVN 119
Query: 127 YARFREERMFDCDKF--MSKTEFLLNQLRSKFDDYNYLQQQINKHRNEIELREGETFETL 184
++ FR+ + ++ M+ + LL N + ++++ + E+E E + +++
Sbjct: 120 FSMFRDNKRHVYERVCQMNDEKLLLK---------NEIDEKVHNAKKELERCERDARKSI 170
Query: 185 QQQNKHLDQQISRLKG-------LQETLNIDYNAYKSRKQSLLHDLEKFGFELIELEFER 237
++ K ++++IS L+ Q L + K+ + L ++ L E
Sbjct: 171 EEA-KGVEEEISLLESELKDFYRHQRALVQETERSKTERNEYSDKLSSLKLMVLNLNQEI 229
Query: 238 NKLEKHSKTDFNELNLNIEKXXXXXXXXXXXXXXXXXKHRKLRISMNTFQTLTHELYDVL 297
L+ +D +L +++ K L+ + Q L + +
Sbjct: 230 TCLKTQIVSDPTKLMELLDEMRCLIAKESEIMKGLEVKRVGLKEKIEFMQVLKEDTMKAI 289
Query: 298 QL-ISTDLQESHLREAN--LIDMREQLLQTDQKLNNILSSGVMVKMKILQSQLDNQKKKL 354
L IS + + + N + ++ Q+ D +N + +++ + Q+ + + K+
Sbjct: 290 TLAISNREADKTIDKTNREISELEVQMKNLDSGIN-----ALKIRLNHVSRQISHIESKI 344
Query: 355 HQLEGETKAKQIENSETLQLLQRQYTDEVVPDLR-AAEEQVENDLANGTIKNYDYEISQL 413
L+ K E S L+ L+ Y VV D R + ++E ++ K+ +YE+ +
Sbjct: 345 FNLQDNDKRCSEEISAKLEKLKSNY--GVVSDERNSIRGKIEENVR--LTKSIEYELVKR 400
Query: 414 KQAFQTEIDAIDLEYSLLSSHINRYMEE 441
+ +I AI L + Y E
Sbjct: 401 RNEHANDITAIQSALCRLKDDVFNYFAE 428
>At1g61000_1 [D] KOG4438 Centromere-associated protein NUF2
Length = 567
Score = 61.2 bits (147), Expect = 3e-09
Identities = 81/388 (20%), Positives = 171/388 (43%), Gaps = 32/388 (8%)
Query: 1 MSKDVFPLLELSELVTCLQSCDFSLAVEENISKPSSQYVITLYKQIIDTFMGVSPDTLLS 60
MS +P L S+++T L+ + E ++ P+S +V LY +I+ + D L
Sbjct: 1 MSAYEYPRLSRSDIITALKDAQIASVTETDLKTPTSDFVSELYTRIL-----IYLDALDE 55
Query: 61 NEALFDNSGSNDIQQNPAYTETVKVLALNKICFKFFQDIGVSD----FNMMDLYKPDSLR 116
E + + + +NP + T + A+ C K + + D + DL +P+S R
Sbjct: 56 EEKGQVDFEALEQLENPDHHAT-SMQAMKLYC-KVKDMLEMLDCPLPISFKDLLRPESSR 113
Query: 117 TRRLLSAVVNYARFREERMFDCDKFMSKTEFLLNQLRSKFDD-----YNYLQQQINKHRN 171
T +SA++NY +++ +M D + ++ LL++ R + + Y L +I +
Sbjct: 114 TEFFISALLNYGLYKDSKM-DLIRPKAEELGLLDEQRKQCEAKVAQLYMQLNAEIGEFDE 172
Query: 172 EIELREGETFETLQQQNKHLDQQISRLKGLQETLNIDYNAYKSRKQSLLHDLEKFGFELI 231
+E R+ + L+ + L+++I L Q +L + + + + +++ K F+L+
Sbjct: 173 AVE-RDLPFVQELEANIEQLNKKILELNNQQMSLRATFQKMREKSTQMDNEISKAEFDLV 231
Query: 232 ELEFERNKLEKHSKTDFNELNLNIEKXXXXXXXXXXXXXXXXXKHRKLRISMNTFQTLTH 291
E E L ++L +E+ ++ + F+ +T+
Sbjct: 232 ETVQENANLRSQIVQSPDKLQGALEEKKLVLGETKKAEQSAMVTFQEKAAILEVFEKVTN 291
Query: 292 ------ELYDVLQLISTDLQESHLREANLIDMREQLLQTDQKLNNILSSGVMVKMKILQS 345
E + +S D EA +++ RE++ + +Q LN L + K K +
Sbjct: 292 AKTVEKEFKALKDKLSEDGVAYKSLEAKVVE-RERIGKLEQ-LNESLKQ--LEKEKAV-- 345
Query: 346 QLDNQKKKLHQL--EGETKAKQIENSET 371
D+ K+L++L E E++ +++E +T
Sbjct: 346 MFDDWTKQLNELKVEVESRRRELETRQT 373
>Hs7661992 [KRT] KOG4572 Predicted DNA-binding transcription factor interacts
with stathmin
Length = 1591
Score = 50.1 bits (118), Expect = 7e-06
Identities = 65/305 (21%), Positives = 134/305 (43%), Gaps = 38/305 (12%)
Query: 139 DKFMSKTEFLLNQLRSKFDDYNYLQQQINKHRNEIELREGETFETLQQQNKHLDQQISRL 198
D + +TE N+++ + L++ + NE L + E + QN+ DQ++ +
Sbjct: 879 DGLIKETEENENKIKKLKGELVCLEEVLQNKDNEFALVKHEKEAVICLQNEK-DQKLLEM 937
Query: 199 KGLQETLNIDYNAYKSRKQSLLHDLEKFGFELIE-LEFERNKLEKHSKTDFNELNLNIEK 257
+ + + N + K ++ +L DL+K E E L+ R +L+ ++ EL ++
Sbjct: 938 ENIMHSQNCEIKELKQSREIVLEDLKKLHVENDEKLQLLRAELQSLEQSHLKELEDTLQ- 996
Query: 258 XXXXXXXXXXXXXXXXXKH-RKLRISMNTFQTLTHELYDVLQLISTDLQESHLREANLID 316
+H ++ M + EL Q I +QESH A +I
Sbjct: 997 ----------------VRHIQEFEKVMTDHRVSLEELKKENQQIINQIQESH---AEIIQ 1037
Query: 317 MREQLLQTDQKLNNILSSGVMVKMKILQSQLDNQKKKLHQLEGETKAKQIENSETLQLLQ 376
+E+ LQ + KL S K+++ + + + ++ L E++A+Q ETL+ L
Sbjct: 1038 EKEKQLQ-ELKLKVSDLSDTRCKLEVELALKEAETDEIKILLEESRAQQ---KETLKSLL 1093
Query: 377 RQYTDEVVPDLRAAEEQVENDLANGTIKNYDYEISQLKQAFQTEIDAIDLEYSLLSSHIN 436
Q T+ + ++ ++++++ +NY +++L+ E D +S I+
Sbjct: 1094 EQETENLRTEISKLNQKIQDN-----NENYQVGLAELRTLMTIEKD------QCISELIS 1142
Query: 437 RYMEE 441
R+ EE
Sbjct: 1143 RHEEE 1147
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.133 0.355
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,690,327
Number of Sequences: 60738
Number of extensions: 960878
Number of successful extensions: 4686
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 4676
Number of HSP's gapped (non-prelim): 6
length of query: 451
length of database: 30,389,216
effective HSP length: 110
effective length of query: 341
effective length of database: 23,708,036
effective search space: 8084440276
effective search space used: 8084440276
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)