ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI6013 good A KOG0227 RNA processing and modification Splicing factor 3a, subunit 2
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI6013 2100384 2099626 -253
(253 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL043c [A] KOG0227 Splicing factor 3a subunit 2 224 1e-58
SPBC21C3.05 [A] KOG0227 Splicing factor 3a subunit 2 86 5e-17
7294535 [A] KOG0227 Splicing factor 3a subunit 2 86 5e-17
Hs21361376 [A] KOG0227 Splicing factor 3a subunit 2 84 2e-16
CE28910 [A] KOG0227 Splicing factor 3a subunit 2 84 2e-16
HsM6005866 [A] KOG0227 Splicing factor 3a subunit 2 83 3e-16
At2g32600 [A] KOG0227 Splicing factor 3a subunit 2 68 1e-11
>YDL043c [A] KOG0227 Splicing factor 3a subunit 2
Length = 266
Score = 224 bits (570), Expect = 1e-58
Identities = 121/265 (45%), Positives = 169/265 (63%), Gaps = 22/265 (8%)
Query: 1 MDYQNRAXXXXXXXXIASDSQQNLQRRKQVQDLLSNGQDIPFSFQQDQEGSKLRANPDIY 60
M+Y IAS+SQ NLQRRK+V+ LLS G+++P++FQ D++ ++R+NP IY
Sbjct: 1 MNYLEGVGSKKGGGGIASESQFNLQRRKEVESLLSKGENVPYTFQ-DEKDDQVRSNPYIY 59
Query: 61 KNHSGRLVCKLCNTLHVSWSSVERHLTGKKHQLNVLKRG--------GRNGQLKSNTDKV 112
KNHSG+LVCKLCNT+H+SWSSVERHL GKKH LNVL+RG GR GQ
Sbjct: 60 KNHSGKLVCKLCNTMHMSWSSVERHLGGKKHGLNVLRRGISIEKSSLGREGQ------TT 113
Query: 113 RDRNFQHEVDNFRASLQKNGIKPEVKVVKVRHPEKELFGIAVKVRYG---KEQQIHSAEK 169
D Q ++ + SL+ NG P K+ V++P+ G+A++V Y KE + S +K
Sbjct: 114 HDFRQQQKIIEAKQSLKNNGTIPVCKIATVKNPKNGSVGLAIQVNYSSEVKENSVDSDDK 173
Query: 170 DEFIPYLRIVSDFELDKPDPKNVKYLVVAFEPFENVGIQIPSDEI---ELNDYDPSDSLA 226
+ P +RIVS EL K K+LV+A+EPFEN+ I++P +EI E ND D ++
Sbjct: 174 AKVPPLIRIVSGLELSDTKQKGKKFLVIAYEPFENIAIELPPNEILFSENNDMDNNND-G 232
Query: 227 LDELNRKCTYWDQDNGDFYVQLFFR 251
+DELN+KCT+WD + +YVQ FF+
Sbjct: 233 VDELNKKCTFWDAISKLYYVQFFFK 257
>SPBC21C3.05 [A] KOG0227 Splicing factor 3a subunit 2
Length = 217
Score = 85.9 bits (211), Expect = 5e-17
Identities = 67/212 (31%), Positives = 105/212 (48%), Gaps = 26/212 (12%)
Query: 1 MDYQNRAXXXXXXXXIASDSQQNLQRRKQVQDLLSNGQDIPFSFQQDQEGSKLRANPDIY 60
MDYQNRA +A + N RR++++ L D L +P +
Sbjct: 1 MDYQNRAGVRFGGGGVAGYQETNAARRERLRKLALETID-------------LSKDPYLM 47
Query: 61 KNHSGRLVCKLCNTLHVSWSSVERHLTGKKHQLNVLKRGG-RNGQLKSNTDKVRDRNFQH 119
KNH G C+LC T H + +S H GKKHQ N+ +R N + + N +V Q
Sbjct: 48 KNHLGTFECRLCLTTHANENSYLTHTQGKKHQTNLARRQALENKKSQENAPQVLLGISQS 107
Query: 120 EVDNFRASLQKNGIKPEVKVVKVRHPEKELFGIAVKVRYGKEQQIHSAEKDEFIPYLRIV 179
V + S+ K G +P KV K+R E FG+ +++Y + ++++ P RI+
Sbjct: 108 HV-QVKKSVVKIG-RPGYKVSKIREAESGKFGLRFQIKY-PDIEVNAK------PRYRIM 158
Query: 180 SDFE--LDKPDPKNVKYLVVAFEPFENVGIQI 209
S +E ++ PD K +YLVVA EP+E++ +I
Sbjct: 159 SAYEQRVEAPDRK-FQYLVVAAEPYESIAFKI 189
>7294535 [A] KOG0227 Splicing factor 3a subunit 2
Length = 264
Score = 85.9 bits (211), Expect = 5e-17
Identities = 69/259 (26%), Positives = 117/259 (44%), Gaps = 53/259 (20%)
Query: 1 MDYQNRAXXXXXXXXIASDSQQNLQRRKQVQDLLSNGQDIPFSFQQDQEGSKLRANPDIY 60
MD+QNRA +AS S+ N R+++++ L D L +P
Sbjct: 1 MDFQNRAGGKTGSGGVASWSETNRDRKERLRQLALETID-------------LNKDPYFM 47
Query: 61 KNHSGRLVCKLCNTLHVSWSSVERHLTGKKHQLNVLKRGGRNGQLKSNTDKVRDRNFQHE 120
KNH G CKLC TLH + S H GKKHQ N+ +R + + E
Sbjct: 48 KNHLGSYECKLCLTLHNNEGSYLAHTQGKKHQDNLARRAAKEAK---------------E 92
Query: 121 VDNFRASLQKNGIKPEVKVVKVRHP------EKELFGIAVKVRYGKEQQIHSAEKDEFIP 174
+ + +K ++P+ K VK+ P ++EL + + Q + + +P
Sbjct: 93 APSSLLAPEKPRVEPK-KFVKIGRPGYRVTKQRELSNGQQSLLF---QVDYPEITESIVP 148
Query: 175 YLRIVSDFE--LDKPDPKNVKYLVVAFEPFENVGIQIPSDEIELNDYDPSDSLALDELNR 232
R +S +E ++ PD K +YL+ A EP+E +G ++PS E+E ++ +
Sbjct: 149 RHRFMSAYEQKIEPPDRK-WQYLLFAAEPYETIGFKVPSREVEKSE------------GK 195
Query: 233 KCTYWDQDNGDFYVQLFFR 251
T+W++D F++Q F+
Sbjct: 196 FWTHWNRDTKQFFLQFAFK 214
>Hs21361376 [A] KOG0227 Splicing factor 3a subunit 2
Length = 464
Score = 84.0 bits (206), Expect = 2e-16
Identities = 68/253 (26%), Positives = 113/253 (43%), Gaps = 42/253 (16%)
Query: 1 MDYQNRAXXXXXXXXIASDSQQNLQRRKQVQDLLSNGQDIPFSFQQDQEGSKLRANPDIY 60
MD+Q+R +AS S+ N RR++++ L DI +P
Sbjct: 1 MDFQHRPGGKTGSGGVASSSESNRDRRERLRQLALETIDI-------------NKDPYFM 47
Query: 61 KNHSGRLVCKLCNTLHVSWSSVERHLTGKKHQLNVLKRGGRNGQLKSNTDKVRDRNFQHE 120
KNH G CKLC TLH + S H GKKHQ N+ +R + + K + + E
Sbjct: 48 KNHLGSYECKLCLTLHNNEGSYLAHTQGKKHQTNLARRAAK--EAKEAPAQPAPEKVKVE 105
Query: 121 VDNFRASLQKNGIKPEVKVVKVRHPEKELFGIAVKVRYGKEQQIHSAEKDEFIPYLRIVS 180
V F K G +P KV K R E + ++ Y + + +P R +S
Sbjct: 106 VKKF----VKIG-RPGYKVTKQRDSEMGQQSLLFQIDYPE-------IAEGIMPRHRFMS 153
Query: 181 DFE--LDKPDPKNVKYLVVAFEPFENVGIQIPSDEIELNDYDPSDSLALDELNRKCTYWD 238
+E ++ PD + +YL++A EP+E + ++PS EI+ + + T+W+
Sbjct: 154 AYEQRIEPPD-RRWQYLLMAAEPYETIAFKVPSREIDKAE------------GKFWTHWN 200
Query: 239 QDNGDFYVQLFFR 251
++ F++Q F+
Sbjct: 201 RETKQFFLQFHFK 213
>CE28910 [A] KOG0227 Splicing factor 3a subunit 2
Length = 222
Score = 84.0 bits (206), Expect = 2e-16
Identities = 68/261 (26%), Positives = 108/261 (41%), Gaps = 59/261 (22%)
Query: 1 MDYQNRAXXXXXXXXIASDSQQNLQRRKQVQDLLSNGQDIPFSFQQDQEGSKLRANPDIY 60
MD+QNRA +AS + + RR++++ L D L+ +P
Sbjct: 1 MDFQNRAGGKTGSGGVASAADAGVDRRERLRQLALETID-------------LQKDPYFM 47
Query: 61 KNHSGRLVCKLCNTLHVSWSSVERHLTGKKHQLNVLKRGGRNGQLKSNTDKVRDRNFQHE 120
+NH G CKLC TLH + S H GKKHQ N+ +R + +
Sbjct: 48 RNHIGTYECKLCLTLHNNEGSYLAHTQGKKHQANLARRAAK----------------EQS 91
Query: 121 VDNFRASLQKNGI---------KPEVKVVKVRHPEKELFGIAVKVRYGKEQQIHSAEKDE 171
F + QK + +P KV K R P + ++ Y + D
Sbjct: 92 EQPFLPAPQKAAVETKKFVKIGRPGYKVTKERDPGAGQQALLFQIDYPE-------IADG 144
Query: 172 FIPYLRIVSDFELD-KPDPKNVKYLVVAFEPFENVGIQIPSDEIELNDYDPSDSLALDEL 230
P R +S +E +P K +YL+ A EP+E +G +IPS E+ D+
Sbjct: 145 IAPRHRFMSAYEQKIQPPDKRWQYLLFAAEPYETIGFKIPSREV-------------DKS 191
Query: 231 NRKCTYWDQDNGDFYVQLFFR 251
+ T W++D F++Q+ F+
Sbjct: 192 EKFWTMWNKDTKQFFLQVAFK 212
>HsM6005866 [A] KOG0227 Splicing factor 3a subunit 2
Length = 464
Score = 83.2 bits (204), Expect = 3e-16
Identities = 68/253 (26%), Positives = 112/253 (43%), Gaps = 42/253 (16%)
Query: 1 MDYQNRAXXXXXXXXIASDSQQNLQRRKQVQDLLSNGQDIPFSFQQDQEGSKLRANPDIY 60
MD+Q+R +AS S+ N RR+ ++ L DI +P
Sbjct: 1 MDFQHRPGGKTGSGGVASSSESNRDRREPLRQLALETIDI-------------NKDPYFM 47
Query: 61 KNHSGRLVCKLCNTLHVSWSSVERHLTGKKHQLNVLKRGGRNGQLKSNTDKVRDRNFQHE 120
KNH G CKLC TLH + S H GKKHQ N+ +R + + K + + E
Sbjct: 48 KNHLGSYECKLCLTLHNNEGSYLAHTQGKKHQTNLARRAAK--EAKEAPAQPAPEKVKVE 105
Query: 121 VDNFRASLQKNGIKPEVKVVKVRHPEKELFGIAVKVRYGKEQQIHSAEKDEFIPYLRIVS 180
V F K G +P KV K R E + ++ Y + + +P R +S
Sbjct: 106 VKKF----VKIG-RPGYKVTKQRDSEMGQQSLLFQIDYPE-------IAEGIMPRHRFMS 153
Query: 181 DFE--LDKPDPKNVKYLVVAFEPFENVGIQIPSDEIELNDYDPSDSLALDELNRKCTYWD 238
+E ++ PD + +YL++A EP+E + ++PS EI+ + + T+W+
Sbjct: 154 AYEQRIEPPD-RRWQYLLMAAEPYETIAFKVPSREIDKAE------------GKFWTHWN 200
Query: 239 QDNGDFYVQLFFR 251
++ F++Q F+
Sbjct: 201 RETKQFFLQFHFK 213
>At2g32600 [A] KOG0227 Splicing factor 3a subunit 2
Length = 275
Score = 68.2 bits (165), Expect = 1e-11
Identities = 46/164 (28%), Positives = 78/164 (47%), Gaps = 15/164 (9%)
Query: 53 LRANPDIYKNHSGRLVCKLCNTLHVSWSSVERHLTGKKHQLNVLKRGGRNGQLKSNTDKV 112
L +P +NH G CKLC TLH + + H GK+HQ N+ KR R + +
Sbjct: 38 LAKDPYFMRNHLGSYECKLCLTLHNNEGNYLAHTQGKRHQTNLAKRAAREAKDAPTKPQP 97
Query: 113 RDRNFQHEVDNFRASLQKNGIKPEVKVVKVRHPEKELFGIAVKVRYGKEQQIHSAEKDEF 172
RN + R +++ +P +V K PE + + ++ Y + + D
Sbjct: 98 LKRNV-----SVRRTVKIG--RPGYRVTKQYDPELQQRSLLFQIEYPEIE-------DNI 143
Query: 173 IPYLRIVSDFELD-KPDPKNVKYLVVAFEPFENVGIQIPSDEIE 215
P R +S +E +P K+ +YL+ A EP+E + ++PS E++
Sbjct: 144 KPRHRFMSSYEQKVQPYDKSYQYLLFAAEPYEIIAFKVPSTEVD 187
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,985,202
Number of Sequences: 60738
Number of extensions: 718755
Number of successful extensions: 2002
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1985
Number of HSP's gapped (non-prelim): 7
length of query: 253
length of database: 30,389,216
effective HSP length: 104
effective length of query: 149
effective length of database: 24,072,464
effective search space: 3586797136
effective search space used: 3586797136
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)