ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI6085 good J KOG3123 Translation, ribosomal structure and biogenesis Diphthine synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI6085 2121025 2120132 -298
(298 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR172c [J] KOG3123 Diphthine synthase 522 e-148
SPCC576.14 [J] KOG3123 Diphthine synthase 342 3e-94
Hs7706272 [J] KOG3123 Diphthine synthase 310 2e-84
At4g31790 [J] KOG3123 Diphthine synthase 306 2e-83
CE02109 [J] KOG3123 Diphthine synthase 296 2e-80
7300882 [J] KOG3123 Diphthine synthase 293 2e-79
ECU11g1430 [J] KOG3123 Diphthine synthase 198 9e-51
>YLR172c [J] KOG3123 Diphthine synthase
Length = 300
Score = 522 bits (1345), Expect = e-148
Identities = 252/297 (84%), Positives = 278/297 (92%)
Query: 1 MLYFIGLGLSYETDITVRGLNAIKQCSRVYLEHYTSILMTASLEELEEFYGKKVTLADRE 60
MLY IGLGLSY++DITVRGL AIK+CSRVYLEHYTSILM AS EELE +YGK++ LADRE
Sbjct: 1 MLYLIGLGLSYKSDITVRGLEAIKKCSRVYLEHYTSILMAASQEELESYYGKEIILADRE 60
Query: 61 LVESGAEELLRDADKEDVAFLVVGDVFGATTHTDLVLRAKQRNIPVEVIHNASVMNAVGS 120
LVE+G++++L +ADKEDVAFLVVGD FGATTHTDLVLRAK+ IPVE+IHNASVMNAVG+
Sbjct: 61 LVETGSKQILNNADKEDVAFLVVGDPFGATTHTDLVLRAKREAIPVEIIHNASVMNAVGA 120
Query: 121 CGLQLYNFGQTISMVFFTDSWRPDSWYDKIWENRKIGLHTLVLLDIKVKEQSIENMARGR 180
CGLQLYNFGQT+SMVFFTD+WRPDSWYDKIWENRKIGLHTLVLLDIKVKEQSIENMARGR
Sbjct: 121 CGLQLYNFGQTVSMVFFTDNWRPDSWYDKIWENRKIGLHTLVLLDIKVKEQSIENMARGR 180
Query: 181 LIYEPPRYMSIAQCCEQLLEIEETRGTEAYTPDTPCVAISRLGSASQTFKAGTIKELAEY 240
LIYEPPRYMSIAQCCEQLLEIEE RGT+AYTPDTP VAISRLGS+SQ+FK+GTI ELA Y
Sbjct: 181 LIYEPPRYMSIAQCCEQLLEIEEKRGTKAYTPDTPAVAISRLGSSSQSFKSGTISELANY 240
Query: 241 DSGEPLHSLVILGRQTHELEIEYLLEFCDDREKFKQDVASDQEYFKPPAWVPPPEDE 297
DSGEPLHSLVILGRQ HELE+EYLLEF DD+EKF +DVA+DQEYFKP AWVPP ED+
Sbjct: 241 DSGEPLHSLVILGRQCHELELEYLLEFADDKEKFGKDVANDQEYFKPAAWVPPTEDD 297
>SPCC576.14 [J] KOG3123 Diphthine synthase
Length = 283
Score = 342 bits (878), Expect = 3e-94
Identities = 167/274 (60%), Positives = 213/274 (76%), Gaps = 1/274 (0%)
Query: 1 MLYFIGLGLSYETDITVRGLNAIKQCSRVYLEHYTSILMTASLEELEEFYGKKVTLADRE 60
M Y IGLGL E DIT+RGL +K+C R+YLE YTSIL+ E+LEE YGK+V LADRE
Sbjct: 1 MFYLIGLGLFDEKDITLRGLETVKKCQRIYLEAYTSILLVQK-EKLEELYGKEVILADRE 59
Query: 61 LVESGAEELLRDADKEDVAFLVVGDVFGATTHTDLVLRAKQRNIPVEVIHNASVMNAVGS 120
+VES ++E+L+DAD DVA LVVGD GATTH DLV+RA++ IPV +IHNAS+MNA+G+
Sbjct: 60 MVESSSDEILKDADNCDVAMLVVGDPMGATTHADLVIRARELKIPVRMIHNASIMNAIGA 119
Query: 121 CGLQLYNFGQTISMVFFTDSWRPDSWYDKIWENRKIGLHTLVLLDIKVKEQSIENMARGR 180
CGLQLY FGQT+S+VFF +++RP S+YD I EN +GLHTLVLLDIKVKEQS EN+ARGR
Sbjct: 120 CGLQLYKFGQTVSLVFFENNYRPQSFYDHIKENVSLGLHTLVLLDIKVKEQSWENLARGR 179
Query: 181 LIYEPPRYMSIAQCCEQLLEIEETRGTEAYTPDTPCVAISRLGSASQTFKAGTIKELAEY 240
+YEPPRYMS + +Q+LE+EE R TPD+ CVA+ R+GS Q AGT++ELAE+
Sbjct: 180 KVYEPPRYMSASLAAQQMLEVEEDRQENICTPDSLCVAVGRMGSDDQVIFAGTLQELAEH 239
Query: 241 DSGEPLHSLVILGRQTHELEIEYLLEFCDDREKF 274
D G PLHS+V++GR H+LE+E+L + + E F
Sbjct: 240 DIGPPLHSVVLVGRDVHDLELEFLRAYAVNLENF 273
>Hs7706272 [J] KOG3123 Diphthine synthase
Length = 297
Score = 310 bits (794), Expect = 2e-84
Identities = 161/283 (56%), Positives = 204/283 (71%), Gaps = 17/283 (6%)
Query: 1 MLYFIGLGLSYETDITVRGLNAIKQCSRVYLEHYTSILMTASLEELEEFYGKKVTLADRE 60
MLY IGLGL DITV+GL +++CSRVYLE YTS+L T E LEEFYG+K+ +ADRE
Sbjct: 1 MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVL-TVGKEALEEFYGRKLVVADRE 59
Query: 61 LVESGAEELLRDADKEDVAFLVVGDVFGATTHTDLVLRAKQRNIPVEVIHNASVMNAVGS 120
VE A+ +L+DAD DVAFLVVGD FGATTH+DLVLRA + IP VIHNAS+MNAVG
Sbjct: 60 EVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGC 119
Query: 121 CGLQLYNFGQTIS------------MVFFTDSWRPDSWYDKIWENRKIGLHTLVLLDIKV 168
CGLQLY FG+T+S +V D+WRP+S++DK+ +NR+ G+HTL LLDIKV
Sbjct: 120 CGLQLYKFGETVSIMLISVMLHSLWLVIHLDTWRPESFFDKVKKNRQNGMHTLCLLDIKV 179
Query: 169 KEQSIENMARGRLIYEPPRYMSIAQCCEQLLEI---EETRGTE-AYTPDTPCVAISRLGS 224
KEQS+EN+ +GR IYEPPRYMS+ Q +QLLEI + RG E A T +T CV ++R+G+
Sbjct: 180 KEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGA 239
Query: 225 ASQTFKAGTIKELAEYDSGEPLHSLVILGRQTHELEIEYLLEF 267
Q AGT++++ D GEPLHSL+I G H +E+E L F
Sbjct: 240 DDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLF 282
>At4g31790 [J] KOG3123 Diphthine synthase
Length = 277
Score = 306 bits (785), Expect = 2e-83
Identities = 158/272 (58%), Positives = 201/272 (73%), Gaps = 7/272 (2%)
Query: 1 MLYFIGLGLSYETDITVRGLNAIKQCSRVYLEHYTSILMTA----SLEELEEFYGKKVTL 56
MLY IGLGL E DIT+RGL A+K+ +VY+E YTS+L L LE+FYGK + L
Sbjct: 1 MLYIIGLGLGDEKDITLRGLEAVKKSQKVYMEAYTSLLSFGLSADGLSNLEKFYGKPIIL 60
Query: 57 ADRELVESGAEELLRDADKEDVAFLVVGDVFGATTHTDLVLRAKQRNIPVEVIHNASVMN 116
ADRE+VE A +++ +A DVAFLVVGD FGATTH+DLV+RAK + VEV+HNASVMN
Sbjct: 61 ADREMVEEKAGDMIDEAIDNDVAFLVVGDPFGATTHSDLVVRAKTLGVKVEVVHNASVMN 120
Query: 117 AVGSCGLQLYNFGQTISMVFFTDSWRPDSWYDKIWENRKIGLHTLVLLDIKVKEQSIENM 176
AVG CGLQLY++G+T+S+ FFT++WRPDS+Y+KI +NR +GLHTL LLDI+VKE + E++
Sbjct: 121 AVGICGLQLYHYGETVSIPFFTETWRPDSFYEKIKKNRSLGLHTLCLLDIRVKEPTFESL 180
Query: 177 AR-GRLIYEPPRYMSIAQCCEQLLEIEETRGTEAYTPDTPCVAISRLGSASQTFKAGTIK 235
R G+ YEPPRYMS+ EQLLE+E+ G Y DT CV +RLGS QT AGT+K
Sbjct: 181 CRGGKKQYEPPRYMSVNTAIEQLLEVEQKHGDSVYGEDTQCVGFARLGSEDQTIVAGTMK 240
Query: 236 ELAEYDSGEPLHSLVILGRQTHELEIEYLLEF 267
+L D G PLH LVI+G +TH +E E +LEF
Sbjct: 241 QLESVDFGAPLHCLVIVG-ETHPVE-EEMLEF 270
>CE02109 [J] KOG3123 Diphthine synthase
Length = 274
Score = 296 bits (758), Expect = 2e-80
Identities = 153/272 (56%), Positives = 198/272 (72%), Gaps = 6/272 (2%)
Query: 1 MLYFIGLGLSYETDITVRGLNAIKQCSRVYLEHYTSILMTA-SLEELEEFYGKKVTLADR 59
+ Y IGLGL DITV+GLN +K C+RV+LE YTSIL LE+FYG+++ ADR
Sbjct: 2 VFYLIGLGLGDVEDITVKGLNIVKNCARVHLEAYTSILCYGLDKTNLEKFYGREIIEADR 61
Query: 60 ELVESGAEELLRDADKEDVAFLVVGDVFGATTHTDLVLRAKQRNIPVEVIHNASVMNAVG 119
+VE ++ +L ADKEDVA LVVGD FGATTH DLVLRAKQ+NIPV+VIHNAS+MNAVG
Sbjct: 62 TVVEQESDAILNGADKEDVALLVVGDPFGATTHADLVLRAKQQNIPVKVIHNASIMNAVG 121
Query: 120 SCGLQLYNFGQTISMVFFTDSWRPDSWYDKIWENRKIGLHTLVLLDIKVKEQSIENMARG 179
CGLQLYNFG+T+S+V +TD W+P+S+YDKI NRK G+HTL LLDIK KEQ++ENM RG
Sbjct: 122 CCGLQLYNFGETVSIVMWTDEWQPESYYDKIALNRKRGMHTLCLLDIKTKEQTVENMMRG 181
Query: 180 RLIYEPPRYMSIAQCCEQLLEIEETRGTE----AYTPDTPCVAISRLGSASQTFKAGTIK 235
R I+EP RY ++ QLL I E R + AY +T V ++R+G +Q ++K
Sbjct: 182 RKIFEPARYQKCSEAARQLLTIYERRKAKGEECAYDENTMVVGLARVGWDNQKIVYASMK 241
Query: 236 ELAEYDSGEPLHSLVILGRQTHELEIEYLLEF 267
+++E + GEPLHSL+I G +TH LE++ L F
Sbjct: 242 DMSEMEMGEPLHSLIIPG-ETHPLEVDMLETF 272
>7300882 [J] KOG3123 Diphthine synthase
Length = 558
Score = 293 bits (750), Expect = 2e-79
Identities = 147/271 (54%), Positives = 195/271 (71%), Gaps = 5/271 (1%)
Query: 1 MLYFIGLGLSYETDITVRGLNAIKQCSRVYLEHYTSILMTASLEELEEFYGKKVTLADRE 60
M Y IGLGL DITV+GL +KQCSRVYLE YTSIL SLE+++EFYG+ + LADR+
Sbjct: 278 MFYLIGLGLGDLKDITVKGLEIVKQCSRVYLEMYTSIL-GCSLEDMQEFYGRPLLLADRD 336
Query: 61 LVESGAEELLRDADKEDVAFLVVGDVFGATTHTDLVLRAKQRNIPVEVIHNASVMNAVGS 120
LVE GA+E+L A + DVA LVVGD FGATTHTD +LRAK++NIP +VIHNAS+MNAVG
Sbjct: 337 LVEQGADEILAGAGESDVALLVVGDPFGATTHTDFILRAKEKNIPYKVIHNASIMNAVGC 396
Query: 121 CGLQLYNFGQTISMVFFTDSWRPDSWYDKIWENRKIGLHTLVLLDIKVKEQSIENMARGR 180
CGLQLY FG+T+S+ ++ ++W+PDS+YDKI NR +HTL LLDIKVKE + E++ R R
Sbjct: 397 CGLQLYKFGETVSIPYWDETWKPDSFYDKIKLNRLHNMHTLCLLDIKVKEPTPESLMRKR 456
Query: 181 LIYEPPRYMSIAQCCEQLLEIEETRGT----EAYTPDTPCVAISRLGSASQTFKAGTIKE 236
Y PPR+M++A+ QLL I E + + + CV ++R+G SQ GT+ E
Sbjct: 457 KEYMPPRFMTVAEAAHQLLSIVEKKDSLEKNTVLNEQSLCVGLARVGQESQQIAVGTLLE 516
Query: 237 LAEYDSGEPLHSLVILGRQTHELEIEYLLEF 267
+ D G PLHSL+I ++ H LE+E+L ++
Sbjct: 517 MRSTDLGGPLHSLIIPAKEMHPLEVEFLQQY 547
>ECU11g1430 [J] KOG3123 Diphthine synthase
Length = 262
Score = 198 bits (503), Expect = 9e-51
Identities = 106/251 (42%), Positives = 155/251 (61%), Gaps = 17/251 (6%)
Query: 1 MLYFIGLGLSYETDITVRGLNAIKQCSRVYLEHYTSILMTASLEELEEFYGKKVTLADRE 60
MLY +G GL DIT+RGL A+K + VYLE YTSI SL+ E+ GKKV LADR
Sbjct: 12 MLYLVGFGLHSYKDITLRGLEAVKGSATVYLESYTSI-HGESLDAFEKLIGKKVHLADRA 70
Query: 61 LVESGAEELLRDADKEDVAFLVVGDVFGATTHTDLVLRAKQRNIPVEVIHNASVMNAVGS 120
++E ++++ ++ +E+V+ LVVG ATTH+D+++RAK++ + VEVIHNAS++N +G
Sbjct: 71 MMEQ-TDKIVDESCRENVSLLVVGTPLFATTHSDIMIRAKEKGVDVEVIHNASIINVLGC 129
Query: 121 CGLQLYNFGQTISMVFFTDSWRPDSWYDKIWENRKIGLHTLVLLDIKVKEQSIENMARGR 180
CGL Y+FG+ +S+ +FT+ W+P S+YD I N + LHTL LLDI+ E
Sbjct: 130 CGLYSYSFGRVVSIPYFTERWKPTSFYDNIVRNHQSNLHTLCLLDIRTDED--------- 180
Query: 181 LIYEPPRYMSIAQCCEQLLEIEETRGTEAYTPDTPCVAISRLGSASQTFKAGTIKELAEY 240
R+MS+ + +Q+LE G+ DT A+ R GS S+ G I +L
Sbjct: 181 ------RFMSVNEAVDQILEAAAITGSPLINEDTRIFAVCRFGSPSEEIVYGKIGDLKLR 234
Query: 241 DSGEPLHSLVI 251
G+PLHSL++
Sbjct: 235 SFGDPLHSLIV 245
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.137 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,332,154
Number of Sequences: 60738
Number of extensions: 755757
Number of successful extensions: 2037
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2017
Number of HSP's gapped (non-prelim): 7
length of query: 298
length of database: 30,389,216
effective HSP length: 106
effective length of query: 192
effective length of database: 23,950,988
effective search space: 4598589696
effective search space used: 4598589696
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)