ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI6207 good P KOG1397 Inorganic ion transport and metabolism Ca2+/H+ antiporter VCX1 and related proteins
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI6207 2163234 2164847 538
(538 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
SPCC1795.02c [P] KOG1397 Ca2+/H+ antiporter VCX1 and related pro... 129 1e-29
YDL128w [P] KOG1397 Ca2+/H+ antiporter VCX1 and related proteins 121 2e-27
At2g38170 [P] KOG1397 Ca2+/H+ antiporter VCX1 and related proteins 101 3e-21
At3g51860 [P] KOG1397 Ca2+/H+ antiporter VCX1 and related proteins 100 6e-21
At3g13320 [P] KOG1397 Ca2+/H+ antiporter VCX1 and related proteins 98 4e-20
At1g55730 [P] KOG1397 Ca2+/H+ antiporter VCX1 and related proteins 94 7e-19
At1g55720 [P] KOG1397 Ca2+/H+ antiporter VCX1 and related proteins 86 1e-16
At5g01490 [P] KOG1397 Ca2+/H+ antiporter VCX1 and related proteins 84 5e-16
>SPCC1795.02c [P] KOG1397 Ca2+/H+ antiporter VCX1 and related proteins
Length = 412
Score = 129 bits (323), Expect = 1e-29
Identities = 105/395 (26%), Positives = 180/395 (44%), Gaps = 39/395 (9%)
Query: 146 VLGTFLQVLRCCFDTRSIVFLPLFPVGIVFYKREEDIPAV-ICXXXXXXXXXXXXSNTTE 204
V+G VL + +VF+P +G++ E + +V I S TE
Sbjct: 49 VVGMTKSVLMSSYFNLMLVFVP---IGLIAGWFEWNAKSVFILNMLAIIPLASLLSFATE 105
Query: 205 YASEHFGPVFGSLLNATFGNLVELIISGTTVSKDDAILTITSLIGSVISNNLLVIGSCFF 264
S GP G+LLNA+FGN +ELI+ + + + + +SL+GS++SN LLV G C
Sbjct: 106 QLSIISGPTLGALLNASFGNAIELIVGVLALKRGELRIVQSSLLGSILSNLLLVFGMCLV 165
Query: 265 FGGLDGVIMHSKAAIANHLFF---LSSVTMALSLTSVIAVIDSGKSLRFKMDTSQIGAAL 321
G+ I +A + LS+ T+ + T ++ D+ S + S+ A +
Sbjct: 166 TTGIRREITTFNITVAQTMIAMLALSTATILIPATFHYSLPDNANSENALLHVSRGTAVI 225
Query: 322 IFLCYIIYLIVSTNQEFKAXXXXXXXXXXXXXXDIETLPELDSDSDFEFSVGXXXXXXXX 381
+ + Y++ L+ + ET P + +G
Sbjct: 226 VLIVYVLLLVFQLKTH-------KHVCHDPSEVEEETEPRI---------LGLRSSIAM- 268
Query: 382 XXXXXXVCFITLAIATAGLGPTANFFVESLKELTDDKPISKVFIGLIILPLAGSLPEHIS 441
LAI T + A++ V S+ +L ++ ISK F+GL+ILP+ G+ EH++
Sbjct: 269 -----------LAIVTVFVSLCADYLVGSIDQLVEEVNISKTFVGLVILPVVGNAAEHVT 317
Query: 442 SLISAHHGQMDLAVSLAIGSSNQVLGMVLPIVQLISSRYPRTGFTLYFEPYVAGYLFSST 501
+++ ++ GQMDLA+ +AIGSS Q+ + P + ++ + TLYFE LF S
Sbjct: 318 AIVVSYRGQMDLALGVAIGSSIQIALFLAPFLVIVGWIISQP-LTLYFESLETVILFVSV 376
Query: 502 FVCCFTLIHGLLFSVNILHGTLLLATFGFIIYLTF 536
F+ + + G + + L G LLA + ++ F
Sbjct: 377 FLVNYLIQDG---ATHWLEGVQLLALYAIVVLAFF 408
>YDL128w [P] KOG1397 Ca2+/H+ antiporter VCX1 and related proteins
Length = 411
Score = 121 bits (304), Expect = 2e-27
Identities = 97/343 (28%), Positives = 156/343 (45%), Gaps = 31/343 (9%)
Query: 200 SNTTEYASEHFGPVFGSLLNATFGNLVELIISGTTVSKDDAILTITSLIGSVISNNLLVI 259
+N TE ++ G G LLNATFGN VELI+S + K + S++GS++SN LLV+
Sbjct: 79 ANATEELADKAGNTIGGLLNATFGNAVELIVSIIALKKGQVRIVQASMLGSLLSNLLLVL 138
Query: 260 GSCFFFGGLDGVIMHSKAAIANHLFFLSSVTMALSL--TSVIAVIDSGKSLRF----KMD 313
G CF FGG + V A + L ++ A L + A + GK F ++
Sbjct: 139 GLCFIFGGYNRVQQTFNQTAAQTMSSLLAIACASLLIPAAFRATLPHGKEDHFIDGKILE 198
Query: 314 TSQIGAALIFLCYIIYLIVSTNQEFKAXXXXXXXXXXXXXXDIETLPELDSDSDFEFSVG 373
S+ + +I + Y+++L F+ D E + + + SV
Sbjct: 199 LSRGTSIVILIVYVLFLY------FQLGSHHALFEQQEEETD-EVMSTISRNPHHSLSVK 251
Query: 374 XXXXXXXXXXXXXXVCFITLAIATAGLGPTANFFVESLKELTDDKPISKVFIGLIILPLA 433
+ L T + A+F V ++ + + +SK FIGLI++P+
Sbjct: 252 SS--------------LVILLGTTVIISFCADFLVGTIDNVVESTGLSKTFIGLIVIPIV 297
Query: 434 GSLPEHISSLISAHHGQMDLAVSLAIGSSNQVLGMVLPIVQLISSRYPRTGFTLYFEPYV 493
G+ EH++S++ A +MDLA+ +AIGSS QV V P + L+ TL F +
Sbjct: 298 GNAAEHVTSVLVAMKDKMDLALGVAIGSSLQVALFVTPFMVLVGWMID-VPMTLNFSTFE 356
Query: 494 AGYLFSSTFVCCFTLIHGLLFSVNILHGTLLLATFGFIIYLTF 536
LF + F+ + ++ G N L G + LA + I F
Sbjct: 357 TATLFIAVFLSNYLILDG---ESNWLEGVMSLAMYILIAMAFF 396
>At2g38170 [P] KOG1397 Ca2+/H+ antiporter VCX1 and related proteins
Length = 463
Score = 101 bits (251), Expect = 3e-21
Identities = 91/346 (26%), Positives = 158/346 (45%), Gaps = 44/346 (12%)
Query: 203 TEYASEHFGPVFGSLLNATFGNLVELIISGTTVSKDDAILTITSLIGSVISNNLLVIGSC 262
TE + + GP G LLNAT GN ELII+ ++ + + SL+GS++SN LLV+G+
Sbjct: 117 TEQLAFYTGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTS 176
Query: 263 FFFGGLDGVIMHS----KAAIANHLFFLSSVTMALSLTSVIAVIDSGKSLRFKMDTSQIG 318
F GG+ + K A N L L L ++ + +G++ + Q+
Sbjct: 177 LFCGGIANIRREQRFDRKQADVNFFLLLLGFLCHL-LPLLVGYLKNGEASAAVLSDMQLS 235
Query: 319 AA--------LIFLCYIIYLIVSTNQEFKAXXXXXXXXXXXXXXDIETLPELDSDSDFEF 370
+ + ++ Y+++ + + Q F A E E D D + E
Sbjct: 236 ISRGFSIVMLISYIAYLVFQLWTHRQLFDAQ---------------EQEDEYDDDVEQET 280
Query: 371 SVGXXXXXXXXXXXXXXVCFITLAIATAGLGPTANFFVESLKELTDDKPISKVFIGLIIL 430
+V + +TL IA + + V +++E +D +S FI +I+L
Sbjct: 281 AV------ISFWSGFAWLVGMTLVIAL-----LSEYVVATIEEASDKWNLSVSFISIILL 329
Query: 431 PLAGSLPEHISSLISAHHGQMDLAVSLAIGSSNQVLGMVLPIVQLISSRYPRTGFTLYFE 490
P+ G+ EH ++I A ++D+++ +A+GS+ Q+ V+P+ +I + L F
Sbjct: 330 PIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQIGLFVVPLT-IIVAWILGINMDLNFG 388
Query: 491 PYVAGYLFSSTFVCCFTLIHGLLFSVNILHGTLLLATFGFIIYLTF 536
P G L S + FTL G S + + G +LL + FII + F
Sbjct: 389 PLETGCLAVSIIITAFTLQDG---SSHYMKGLVLLLCY-FIIAICF 430
>At3g51860 [P] KOG1397 Ca2+/H+ antiporter VCX1 and related proteins
Length = 448
Score = 100 bits (249), Expect = 6e-21
Identities = 77/286 (26%), Positives = 136/286 (46%), Gaps = 35/286 (12%)
Query: 203 TEYASEHFGPVFGSLLNATFGNLVELIISGTTVSKDDAILTITSLIGSVISNNLLVIGSC 262
TE + + GP G LLNAT GN ELII+ ++ + + SL+GS++SN LLV+G+
Sbjct: 106 TEQLAFYTGPTVGGLLNATCGNATELIIAILALANNKVAVVKYSLLGSILSNLLLVLGTS 165
Query: 263 FFFGGLDGVIMHS----KAAIANHLFFLSSVTMAL--SLTSVIAVIDSGKSLRFKMD--- 313
FFGG+ + K A N L + L L A + S+ KM
Sbjct: 166 LFFGGIANIRREQRFDRKQADVNFFLLLMGLLCHLLPLLLKYAATGEVSTSMINKMSLTL 225
Query: 314 --TSQIGAALIFLCYIIYLIVSTNQEFKAXXXXXXXXXXXXXXDIETLPELDSDSDFEFS 371
TS I + ++ Y+I+ + + Q F+A +E P + S F +
Sbjct: 226 SRTSSIVMLIAYIAYLIFQLWTHRQLFEA--QQDDDDAYDDEVSVEETPVIGFWSGFAWL 283
Query: 372 VGXXXXXXXXXXXXXXVCFITLAIATAGLGPTANFFVESLKELTDDKPISKVFIGLIILP 431
VG +T+ IA + + V+++++ +D +S FI +I+LP
Sbjct: 284 VG-----------------MTIVIAL-----LSEYVVDTIEDASDSWGLSVSFISIILLP 321
Query: 432 LAGSLPEHISSLISAHHGQMDLAVSLAIGSSNQVLGMVLPIVQLIS 477
+ G+ EH ++I A ++D+++ +A+GS+ Q+ V+P+ +++
Sbjct: 322 IVGNAAEHAGAIIFAFKNKLDISLGVALGSATQISLFVVPLSVIVA 367
>At3g13320 [P] KOG1397 Ca2+/H+ antiporter VCX1 and related proteins
Length = 439
Score = 97.8 bits (242), Expect = 4e-20
Identities = 90/335 (26%), Positives = 150/335 (43%), Gaps = 39/335 (11%)
Query: 203 TEYASEHFGPVFGSLLNATFGNLVELIISGTTVSKDDAILTITSLIGSVISNNLLVIGSC 262
TE + + GP G LLNATFGN+ ELIIS + + +L+GS++SN LLV+G
Sbjct: 119 TEQLACYTGPTVGGLLNATFGNVTELIISIFALKNGMIRVVQLTLLGSILSNMLLVLGCA 178
Query: 263 FFFGGL-----DGVIMHSKAAIANHLFFLSSVTMALSLTSVI----AVIDSGKSLRFKMD 313
FF GGL D V A + + L ++ M + +V+ + + +G S ++
Sbjct: 179 FFCGGLVFYQKDQVFDKGIATVNSGLLLMA--VMGILFPAVLHYTHSEVHAGSS---ELA 233
Query: 314 TSQIGAALIFLCYIIYLIVSTNQEFKAXXXXXXXXXXXXXXDIETLPELDSDSDFEFSVG 373
S+ + ++ + Y YL + K+ + ET E D D E S
Sbjct: 234 LSRFSSCIMLIAYAAYLFF----QLKSQSNSYSPLDEESNQNEETSAE---DEDPEISKW 286
Query: 374 XXXXXXXXXXXXXXVCFITLAIATAGLGPTANFFVESLKELTDDKPISKVFIGLIILPLA 433
I L+I TA + + + V++++ + I FI I+LP+
Sbjct: 287 EA--------------IIWLSILTAWVSLLSGYLVDAIEGASVSWNIPIAFISTILLPIV 332
Query: 434 GSLPEHISSLISAHHGQMDLAVSLAIGSSNQVLGMVLPIVQLISSRYPRTGFTLYFEPYV 493
G+ EH +++ A ++DL++ +AIGSS Q+ +P +I + L F+ +
Sbjct: 333 GNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQISMFAVPFCVVIGWMMGQQ-MDLNFQLFE 391
Query: 494 AGYLFSSTFVCCFTLIHGLLFSVNILHGTLLLATF 528
LF + V F L G S N G +L+ +
Sbjct: 392 TAMLFITVIVVAFFLQEG---SSNYFKGLMLILCY 423
>At1g55730 [P] KOG1397 Ca2+/H+ antiporter VCX1 and related proteins
Length = 424
Score = 93.6 bits (231), Expect = 7e-19
Identities = 88/335 (26%), Positives = 149/335 (44%), Gaps = 39/335 (11%)
Query: 203 TEYASEHFGPVFGSLLNATFGNLVELIISGTTVSKDDAILTITSLIGSVISNNLLVIGSC 262
TE + + G G LLNATFGN+ ELIIS + + +L+GS++SN LLV+G
Sbjct: 104 TEQLACYTGSTVGGLLNATFGNVTELIISIFALKSGMIRVVQLTLLGSILSNMLLVLGCA 163
Query: 263 FFFGGL-----DGVIMHSKAAIANHLFFLSSVTMALSLTSVI----AVIDSGKSLRFKMD 313
FF GGL + V A + + L ++ M L +V+ + + +G S ++
Sbjct: 164 FFCGGLVFSQKEQVFDKGNAVVNSGLLLMA--VMGLLFPAVLHYTHSEVHAGSS---ELA 218
Query: 314 TSQIGAALIFLCYIIYLIVSTNQEFKAXXXXXXXXXXXXXXDIETLPELDSDSDFEFSVG 373
S+ + ++ + Y YL + K+ + ET D D D E S
Sbjct: 219 LSRFSSCIMLVAYAAYLFF----QLKSQPSSYTPLTEETNQNEETS---DDDEDPEISKW 271
Query: 374 XXXXXXXXXXXXXXVCFITLAIATAGLGPTANFFVESLKELTDDKPISKVFIGLIILPLA 433
I L+I TA + + + V++++ + I FI +I+LP+
Sbjct: 272 EA--------------IIWLSILTAWVSLLSGYLVDAIEGASVSWKIPISFISVILLPIV 317
Query: 434 GSLPEHISSLISAHHGQMDLAVSLAIGSSNQVLGMVLPIVQLISSRYPRTGFTLYFEPYV 493
G+ EH +++ A ++DL++ +AIGSS Q+ +P +I L F+ +
Sbjct: 318 GNAAEHAGAIMFAMKDKLDLSLGVAIGSSIQISMFAVPFCVVIGWMM-GAQMDLNFQLFE 376
Query: 494 AGYLFSSTFVCCFTLIHGLLFSVNILHGTLLLATF 528
LF + V F L G + N G +L+ +
Sbjct: 377 TATLFITVIVVAFFLQEG---TSNYFKGLMLILCY 408
>At1g55720 [P] KOG1397 Ca2+/H+ antiporter VCX1 and related proteins
Length = 401
Score = 85.9 bits (211), Expect = 1e-16
Identities = 73/281 (25%), Positives = 129/281 (44%), Gaps = 34/281 (12%)
Query: 203 TEYASEHFGPVFGSLLNATFGNLVELIISGTTVSKDDAILTITSLIGSVISNNLLVIGSC 262
TE S + G G LLNATFGN++ELIIS + + +L+GS++SN LLV+G
Sbjct: 73 TEQLSCYTGATVGGLLNATFGNVIELIISIIALKNGMIRVVQLTLLGSILSNILLVLGCA 132
Query: 263 FFFGGL-----DGVIMHSKAAIANHLFFLSSVTMALS--LTSVIAVIDSGKSLRFKMDTS 315
FF GGL D V A +++ + ++ + + L + + +G S ++ S
Sbjct: 133 FFCGGLVFPGKDQVFDKRNAVVSSGMLLMAVMGLLFPTFLHYTHSEVHAGSS---ELALS 189
Query: 316 QIGAALIFLCYIIYLIVSTNQEFKAXXXXXXXXXXXXXXDIETLPELDSDSDFEFSVGXX 375
+ + ++ + Y YL + D + PE+ S +E
Sbjct: 190 RFISCIMLVAYAAYLFFQLKSQPSFYTEKTNQNEETSNDDED--PEI---SKWE------ 238
Query: 376 XXXXXXXXXXXXVCFITLAIATAGLGPTANFFVESLKELTDDKPISKVFIGLIILPLAGS 435
I L+I TA + + + V++++ + I FI +I+LP+ G+
Sbjct: 239 -------------AIIWLSIFTAWVSLLSGYLVDAIEGTSVSWKIPISFISVILLPIVGN 285
Query: 436 LPEHISSLISAHHGQMDLAVSLAIGSSNQVLGMVLPIVQLI 476
EH +++ A ++DL++ +AIGSS Q+ +P +I
Sbjct: 286 AAEHAGAIMFAMKDKLDLSLGVAIGSSIQISMFAVPFCVVI 326
>At5g01490 [P] KOG1397 Ca2+/H+ antiporter VCX1 and related proteins
Length = 442
Score = 84.0 bits (206), Expect = 5e-16
Identities = 72/325 (22%), Positives = 139/325 (42%), Gaps = 29/325 (8%)
Query: 160 TRSIVFLPLFPVGIVFYKREEDIPAVICXXXXXXX-XXXXXSNTTEYASEHFGPVFGSLL 218
T+ + P P+ +V ++ + V S TE + H GP L+
Sbjct: 74 TKLFILFPAVPLAVVAHRYDCPRAWVFALSLLGLTPLAERISFLTEQIAFHTGPT--GLM 131
Query: 219 NATFGNLVELIISGTTVSKDDAILTITSLIGSVISNNLLVIGSCFFFGGLDGVIMHSK-- 276
NAT GN E+II+ V + + SL+GS++SN L V+G+ F GG+ + H
Sbjct: 132 NATCGNATEMIIAILAVGQRKMRIVKLSLLGSILSNLLFVLGTSLFLGGISNLRKHQSFD 191
Query: 277 -AAIANHLFFLSSVTMALSLT---SVIAVIDSGKSLRFKMDTSQIGAALIFLCYIIYLIV 332
+ + L +L+ + L + ++ A G + S + +L ++I+ +
Sbjct: 192 PGDMNSMLLYLALLCQTLPMIMRFTMEAEEYDGSDVVVLSRASSFVMLIAYLAFLIFHLF 251
Query: 333 STNQEFKAXXXXXXXXXXXXXXDIETLPELDSDSDFEFSVGXXXXXXXXXXXXXXVCFIT 392
S++ LP+ + D + S
Sbjct: 252 SSH----------------LSPPPPPLPQREDVHDDDVS----DKEEEGAVIGMWSAIFW 291
Query: 393 LAIATAGLGPTANFFVESLKELTDDKPISKVFIGLIILPLAGSLPEHISSLISAHHGQMD 452
L I T + +++ V ++++ D +S FIG+I+LP+ G+ EH ++I A ++D
Sbjct: 292 LIIMTLLVALLSDYLVSTIQDAADSWGLSVGFIGIILLPIVGNAAEHAGAVIFAFRNKLD 351
Query: 453 LAVSLAIGSSNQVLGMVLPIVQLIS 477
+ + +A+GS+ Q+ V+P+ L++
Sbjct: 352 ITLGIALGSATQIALFVVPVTVLVA 376
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.324 0.139 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,994,737
Number of Sequences: 60738
Number of extensions: 1085861
Number of successful extensions: 3281
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 3265
Number of HSP's gapped (non-prelim): 13
length of query: 538
length of database: 30,389,216
effective HSP length: 111
effective length of query: 427
effective length of database: 23,647,298
effective search space: 10097396246
effective search space used: 10097396246
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)