ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactVI6292 good E KOG1549 Amino acid transport and metabolism Cysteine desulfurase NFS1
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactVI6292 2191499 2192842 448
(448 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
SPBC660.12c [E] KOG1549 Cysteine desulfurase NFS1 181 2e-45
At5g26595 [E] KOG1549 Cysteine desulfurase NFS1 111 2e-24
At3g62130 [E] KOG1549 Cysteine desulfurase NFS1 102 9e-22
ECU11g1770 [E] KOG1549 Cysteine desulfurase NFS1 53 8e-07
YCL017c [E] KOG1549 Cysteine desulfurase NFS1 50 7e-06
>SPBC660.12c [E] KOG1549 Cysteine desulfurase NFS1
Length = 392
Score = 181 bits (459), Expect = 2e-45
Identities = 122/422 (28%), Positives = 207/422 (48%), Gaps = 46/422 (10%)
Query: 6 FGHEFKAKYFTYLDSEVTLLNHGSFGTTPTMVLDAAKKSLEEHEKYP---CRDYFLTFIE 62
+GHE K + LD + +N+GS GT V + + L+E + P C Y ++E
Sbjct: 7 YGHEMKKHFM--LDPDYVNVNNGSCGTESLAVYNKHVQLLKEAQSKPDFMCNAYMPMYME 64
Query: 63 EYKRQLQLVAKYLDLEYKNCAFVTNATTGVNTVLRSIPFNFSKDKILFHSTTYGACANTV 122
+ + VAK + + N F +AT G++TVL + P+ D+IL + Y C
Sbjct: 65 ATRNE---VAKLIGADSSNIVFCNSATDGISTVLLTFPWE-QNDEILMLNVAYPTCTYAA 120
Query: 123 KFLHDYFGLQYDVIDINYPCGNDVIVQDFEKRLQTKEYKVCLFDMITSQPGATLPYKELI 182
F + L+ DVID+ D+ +++ E+R + + + D+++S P P+++++
Sbjct: 121 DFAKNQHNLRLDVIDVGVEIDEDLFLKEVEQRFLQSKPRAFICDILSSMPVILFPWEKVV 180
Query: 183 QLCRKYGTWSLVDGAHAAGQVDFAFVNELKPDFLTTNLHKWLSCPKSVALLYVDPKHHVM 242
+LC+KY S++DGAHA G + N + PDFL TN HKWL+ P + +LYV K+H +
Sbjct: 181 KLCKKYNIVSIIDGAHAIGHIPMNLAN-VDPDFLFTNAHKWLNSPAACTVLYVSAKNHNL 239
Query: 243 IQTIPISHNYTAPACQYVEGDDGHNSNILVNKFAFIGTVSYSSYFAVEEALKFRKDICGG 302
I+ +P+S+ Y + + D L N+F + AV EA+KFRK I GG
Sbjct: 240 IEALPLSYGYGLREKESIAVD------TLTNRFVNSFKQDLPKFIAVGEAIKFRKSI-GG 292
Query: 303 EEKIREYQWDLQEAAIPKILEVFGQGSVLLENSTNSLRSP---GLFCIELPIKSECQHLI 359
EEKI++Y E A+ G + E T+ ++ P + +E+P+++ I
Sbjct: 293 EEKIQQY---CHEIALK------GAEIISKELGTSFIKPPYPVAMVNVEVPLRN-----I 338
Query: 360 EKMSTDRIYFLNFKSKCDKIMLAEYKCYAPFQINNSHLFIRFSVQVFNEPSDYEKGATII 419
+ T ++++ +Y + F + R S V+ E SD+ A +I
Sbjct: 339 PSIETQKVFW------------PKYNTFLRFMEFKGKFYTRLSGAVYLEESDFYYIAKVI 386
Query: 420 KD 421
KD
Sbjct: 387 KD 388
>At5g26595 [E] KOG1549 Cysteine desulfurase NFS1
Length = 475
Score = 111 bits (277), Expect = 2e-24
Identities = 106/438 (24%), Positives = 188/438 (42%), Gaps = 58/438 (13%)
Query: 15 FTYLDSEVTLLNHGSFGTTPTMVLDAAKKSLEEHEKYPCRDYFLTFIEEYKRQLQLVAKY 74
F++ D + +N+GSFG P+ +L + + P R YF + ++ +
Sbjct: 64 FSHHDPDFARINNGSFGCCPSSILALQRDWQLRFLRQPDRFYFDELKPKISDSRSVIKRL 123
Query: 75 LDLEYKN-CAFVTNATTGVNTVLRSIPFNFSK------DKILFHSTTYGACANTVKFLHD 127
++ E+ + + V NATT VL+ + F + D ++ YG+ +V+
Sbjct: 124 INAEHDDEVSIVDNATTAAAIVLQQTAWAFREGRFDKGDAVVMLHYAYGSVKKSVEAYVT 183
Query: 128 YFGLQYDVIDINYPC-GNDVIVQDFEKRLQT-----KEYKVCLFDMITSQPGATLPYKEL 181
G + + +P D I+ F L++ + ++ L D +TS P +P KEL
Sbjct: 184 RSGGHVTEVQLPFPVISADEIIDRFRIGLESGKANGRRVRLALIDHVTSMPSVVIPIKEL 243
Query: 182 IQLCRKYGTWSL-VDGAHAAGQVDFAFVNELKPDFLTTNLHKWLSCPKSVALLY------ 234
+++CR+ G + VD AH G VD + E+ DF T+NLHKW P SVA LY
Sbjct: 244 VKICRREGVDQVFVDAAHGIGCVDVD-MKEIGADFYTSNLHKWFFAPPSVAFLYCRKSSN 302
Query: 235 --VDPKHHVMIQTIPISHNYTAPACQYVEGDDGHNSNILVNKFAFIGTVSYSSYFAVEEA 292
V HH + +S+ Y N L + +++GT YS+ V
Sbjct: 303 GGVADLHHPV-----VSNEY---------------GNGLAVESSWVGTRDYSAQLVVPSI 342
Query: 293 LKFRKDICGGEEKIREYQWDLQEAAIPKILEVFGQGSVLLENSTNSLRSPGLFCIELPIK 352
L+F GG + I++ + V G +L+++ L P C + +
Sbjct: 343 LEFVNRFEGGIDGIKKRNHE----------SVVEMGQMLVKSWGTQLGCPPEMCASMIMV 392
Query: 353 SECQHLIEKMSTDRIYFLNFKSKCDKIMLAEY-KCYAPFQINNSHLFIRFSVQVFNEPSD 411
L +D + F + +I + Y + +I+ ++R S QV+N+P D
Sbjct: 393 GLPVCLGVSSESDVLKLRTFLREKFRIEIPIYFRPPGDGEIDPITGYVRISFQVYNKPED 452
Query: 412 YEKGATIIKDIIYGVLEE 429
Y + ++D I G++ +
Sbjct: 453 YHR----LRDAINGLVRD 466
>At3g62130 [E] KOG1549 Cysteine desulfurase NFS1
Length = 454
Score = 102 bits (255), Expect = 9e-22
Identities = 111/439 (25%), Positives = 183/439 (41%), Gaps = 63/439 (14%)
Query: 15 FTYLDSEVTLLNHGSFGTTPTMVLDAAKKSLEEHEKYPCRDYFLTFIEEYKRQLQLVAKY 74
F + + V +N+GSFG P VL+A ++ + + P YF +++
Sbjct: 37 FAHHQTGVARINNGSFGCCPGSVLEAQREWQLRYLRQPDEFYFNGLRRGLLASRTVISDL 96
Query: 75 LDLE-YKNCAFVTNATTGVNTVLRSIPFNFSKDK-------ILFHSTTYGACANTVKFLH 126
++ + + V NATT VL+ + FS+ K ++FH + ++
Sbjct: 97 INADDVDEVSLVDNATTAAAIVLQKVGRCFSEGKYKKEDTVVMFHCAFQSVKKSIQAYVS 156
Query: 127 DYFGLQYDVIDINYPCG-NDVIVQDFEKRLQT-----KEYKVCLFDMITSQPGATLPYKE 180
G +V + +P N+ I+ F + L+ + ++ + D ITS P +P +E
Sbjct: 157 RVGGSTVEV-RLPFPVNSNEEIISKFREGLEKGRANGRTVRLAIIDHITSMPCVLMPVRE 215
Query: 181 LIQLCRKYGTWSL-VDGAHAAGQVDFAFVNELKPDFLTTNLHKWLSCPKSVALLYVDPK- 238
L+++CR+ G + VD AHA G V V E+ D+ +NLHKW CP S+A Y +
Sbjct: 216 LVKICREEGVEQVFVDAAHAIGSVKVD-VKEIGADYYVSNLHKWFFCPPSIAFFYCKKRG 274
Query: 239 -----HHVMIQTIPISHNYTAPACQYVEGDDGHNSNILVNKFAFIGTVSYSSYFAVEEAL 293
HH + +SH + N L + A+IGT YSS V +
Sbjct: 275 SESDVHHPV-----VSHEF---------------GNGLPIESAWIGTRDYSSQLVVPSVM 314
Query: 294 KFRKDICGGEEKIREYQWDLQEAAIPKILEVFGQGSVLLENSTNSLRSP-----GLFCIE 348
+F GG E I D E G +L + +L SP G+ I
Sbjct: 315 EFVNRFEGGMEGIMMKNHD----------EAVRMGLMLADAWGTNLGSPPEMCVGMVMIG 364
Query: 349 LPIKSECQHLIEKMSTDRIYF-LNFKSKCDKIMLAEYKCYAPFQINNSHL---FIRFSVQ 404
LP K C E R Y +++ + L + +S L ++R S Q
Sbjct: 365 LPSKL-CVGSDEDAIKLRSYLRVHYSVEVPVFYLGLRDGEEGVKDKDSGLITAYVRISHQ 423
Query: 405 VFNEPSDYEKGATIIKDII 423
V+N+ DYE+ I +++
Sbjct: 424 VYNKTEDYERLRDAITELV 442
>ECU11g1770 [E] KOG1549 Cysteine desulfurase NFS1
Length = 432
Score = 53.1 bits (126), Expect = 8e-07
Identities = 47/184 (25%), Positives = 82/184 (44%), Gaps = 16/184 (8%)
Query: 71 VAKYLDLEYKNCAFVTNATTGVNTVLRSIP-FNFSKDK---ILFHSTTYGACANTVKFLH 126
VA + + K F + AT N L+ + F + K I+ T + +T +
Sbjct: 85 VASLIGCDPKEIIFTSGATESNNLALKGVSGFKLKEGKAAHIITLQTEHKCILDTCR--- 141
Query: 127 DYFGLQYDVIDINY-PCGNDVIVQDFEKRLQTKEYKVCL-FDMITSQPGATLPYKELIQL 184
L+ + +++ Y P GND +V + + KE V + + S+ G P KE+ L
Sbjct: 142 ---NLEENGVEVTYLPVGNDGVVDIDDVKKSIKENTVLVSIGAVNSEIGTVQPLKEIGML 198
Query: 185 CRKYGTWSLVDGAHAAGQVDFAFVNELKPDFLTTNLHKWLSCPKSVALLYVDPKHHVMIQ 244
C++ G D A G++ VNE+ D L+ HK + PK + LYV + ++
Sbjct: 199 CKERGVLFHTDAAQGVGKIQID-VNEMNIDLLSMCAHK-IYGPKGIGALYV--RRRPRVR 254
Query: 245 TIPI 248
+P+
Sbjct: 255 MVPL 258
>YCL017c [E] KOG1549 Cysteine desulfurase NFS1
Length = 497
Score = 50.1 bits (118), Expect = 7e-06
Identities = 44/179 (24%), Positives = 80/179 (44%), Gaps = 10/179 (5%)
Query: 71 VAKYLDLEYKNCAFVTNATTGVNTVLRSIPFNFSKDKILFHST-TYGACANTVKFLHDYF 129
VAK ++ + K F + AT N VL+ +P + K K +T T C L
Sbjct: 151 VAKMINADPKEIIFTSGATESNNMVLKGVPRFYKKTKKHIITTRTEHKCV-----LEAAR 205
Query: 130 GLQYDVIDINYPCGNDVIVQDFEKRLQTKEYKVCLFDM--ITSQPGATLPYKELIQLCRK 187
+ + ++ + +D + D ++ CL + + ++ G P KE+ +CRK
Sbjct: 206 AMMKEGFEVTFLNVDDQGLIDLKELEDAIRPDTCLVSVMAVNNEIGVIQPIKEIGAICRK 265
Query: 188 YGTWSLVDGAHAAGQVDFAFVNELKPDFLTTNLHKWLSCPKSVALLYVDPKHHVMIQTI 246
+ D A A G++ VNE+ D L+ + HK + PK + +YV + V ++ +
Sbjct: 266 NKIYFHTDAAQAYGKIHID-VNEMNIDLLSISSHK-IYGPKGIGAIYVRRRPRVRLEPL 322
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.138 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,091,821
Number of Sequences: 60738
Number of extensions: 1217915
Number of successful extensions: 2677
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 2663
Number of HSP's gapped (non-prelim): 6
length of query: 448
length of database: 30,389,216
effective HSP length: 110
effective length of query: 338
effective length of database: 23,708,036
effective search space: 8013316168
effective search space used: 8013316168
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)